Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
- Autores
- Berra Romani, Roberto; Raqeeb, Abdul; Laforenza, Umberto; Scaffino, Manuela Federica; Moccia, Francesco; Avelino-Cruz, Josè Everardo; Oldani, Amanda; Coltrini, Daniela; Milesi, Verónica; Taglietti, Vanni; Tanzi, Franco
- Año de publicación
- 2008
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The mechanism whereby extracellular Ca2+ exerts the endothelium-dependent control of vascular tone is still unclear. In this study, we assessed whether cardiac microvascular endothelial cells (CMEC) express a functional extracellular Ca2+-sensing receptor (CaSR) using a variety of techniques. CaSR mRNA was detected using RT-PCR, and CaSR protein was identified by immunocytochemical analysis. In order to assess the functionality of the receptor, CMEC were loaded with the Ca2+-sensitive fluorochrome, Fura-2/AM. A number of CaSR agonists, such as spermine, Gd 3+, La3+ and neomycin, elicited a heterogeneous intracellular Ca2+ signal, which was abolished by disruption of inositol 1,4,5-trisphosphate (InsP3) signaling and by depletion of intracellular stores with cyclopiazonic acid. The inhibition of the Na +/Ca2+ exchanger upon substitution of extracellular Na+ unmasked the Ca2+ signal triggered by an increase in extracellular Ca2+ levels. Finally, aromatic amino acids, which function as allosteric activators of CaSR, potentiated the Ca2+ response to the CaSR agonist La3+. These data provide evidence that CMEC express CaSR, which is able to respond to physiological agonists by mobilizing Ca2+ from intracellular InsP3-sensitive stores.
Facultad de Ciencias Exactas - Materia
-
Química
Ca2+-sensing receptor
Cardiac function
Cardiac microvascular endothelial cells
Extracellular Ca2+
Fura-2/AM
Inositol 1,4,5-trisphosphate
Na+/Ca2+ exchanger
Phospholipase C
Vascular tone - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/83531
Ver los metadatos del registro completo
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Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptorBerra Romani, RobertoRaqeeb, AbdulLaforenza, UmbertoScaffino, Manuela FedericaMoccia, FrancescoAvelino-Cruz, Josè EverardoOldani, AmandaColtrini, DanielaMilesi, VerónicaTaglietti, VanniTanzi, FrancoQuímicaCa2+-sensing receptorCardiac functionCardiac microvascular endothelial cellsExtracellular Ca2+Fura-2/AMInositol 1,4,5-trisphosphateNa+/Ca2+ exchangerPhospholipase CVascular toneThe mechanism whereby extracellular Ca2+ exerts the endothelium-dependent control of vascular tone is still unclear. In this study, we assessed whether cardiac microvascular endothelial cells (CMEC) express a functional extracellular Ca2+-sensing receptor (CaSR) using a variety of techniques. CaSR mRNA was detected using RT-PCR, and CaSR protein was identified by immunocytochemical analysis. In order to assess the functionality of the receptor, CMEC were loaded with the Ca2+-sensitive fluorochrome, Fura-2/AM. A number of CaSR agonists, such as spermine, Gd 3+, La3+ and neomycin, elicited a heterogeneous intracellular Ca2+ signal, which was abolished by disruption of inositol 1,4,5-trisphosphate (InsP3) signaling and by depletion of intracellular stores with cyclopiazonic acid. The inhibition of the Na +/Ca2+ exchanger upon substitution of extracellular Na+ unmasked the Ca2+ signal triggered by an increase in extracellular Ca2+ levels. Finally, aromatic amino acids, which function as allosteric activators of CaSR, potentiated the Ca2+ response to the CaSR agonist La3+. These data provide evidence that CMEC express CaSR, which is able to respond to physiological agonists by mobilizing Ca2+ from intracellular InsP3-sensitive stores.Facultad de Ciencias Exactas2008info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf73-82http://sedici.unlp.edu.ar/handle/10915/83531enginfo:eu-repo/semantics/altIdentifier/issn/1018-1172info:eu-repo/semantics/altIdentifier/doi/10.1159/000140677info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:15:40Zoai:sedici.unlp.edu.ar:10915/83531Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:15:41.234SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor |
title |
Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor |
spellingShingle |
Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor Berra Romani, Roberto Química Ca2+-sensing receptor Cardiac function Cardiac microvascular endothelial cells Extracellular Ca2+ Fura-2/AM Inositol 1,4,5-trisphosphate Na+/Ca2+ exchanger Phospholipase C Vascular tone |
title_short |
Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor |
title_full |
Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor |
title_fullStr |
Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor |
title_full_unstemmed |
Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor |
title_sort |
Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor |
dc.creator.none.fl_str_mv |
Berra Romani, Roberto Raqeeb, Abdul Laforenza, Umberto Scaffino, Manuela Federica Moccia, Francesco Avelino-Cruz, Josè Everardo Oldani, Amanda Coltrini, Daniela Milesi, Verónica Taglietti, Vanni Tanzi, Franco |
author |
Berra Romani, Roberto |
author_facet |
Berra Romani, Roberto Raqeeb, Abdul Laforenza, Umberto Scaffino, Manuela Federica Moccia, Francesco Avelino-Cruz, Josè Everardo Oldani, Amanda Coltrini, Daniela Milesi, Verónica Taglietti, Vanni Tanzi, Franco |
author_role |
author |
author2 |
Raqeeb, Abdul Laforenza, Umberto Scaffino, Manuela Federica Moccia, Francesco Avelino-Cruz, Josè Everardo Oldani, Amanda Coltrini, Daniela Milesi, Verónica Taglietti, Vanni Tanzi, Franco |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Química Ca2+-sensing receptor Cardiac function Cardiac microvascular endothelial cells Extracellular Ca2+ Fura-2/AM Inositol 1,4,5-trisphosphate Na+/Ca2+ exchanger Phospholipase C Vascular tone |
topic |
Química Ca2+-sensing receptor Cardiac function Cardiac microvascular endothelial cells Extracellular Ca2+ Fura-2/AM Inositol 1,4,5-trisphosphate Na+/Ca2+ exchanger Phospholipase C Vascular tone |
dc.description.none.fl_txt_mv |
The mechanism whereby extracellular Ca2+ exerts the endothelium-dependent control of vascular tone is still unclear. In this study, we assessed whether cardiac microvascular endothelial cells (CMEC) express a functional extracellular Ca2+-sensing receptor (CaSR) using a variety of techniques. CaSR mRNA was detected using RT-PCR, and CaSR protein was identified by immunocytochemical analysis. In order to assess the functionality of the receptor, CMEC were loaded with the Ca2+-sensitive fluorochrome, Fura-2/AM. A number of CaSR agonists, such as spermine, Gd 3+, La3+ and neomycin, elicited a heterogeneous intracellular Ca2+ signal, which was abolished by disruption of inositol 1,4,5-trisphosphate (InsP3) signaling and by depletion of intracellular stores with cyclopiazonic acid. The inhibition of the Na +/Ca2+ exchanger upon substitution of extracellular Na+ unmasked the Ca2+ signal triggered by an increase in extracellular Ca2+ levels. Finally, aromatic amino acids, which function as allosteric activators of CaSR, potentiated the Ca2+ response to the CaSR agonist La3+. These data provide evidence that CMEC express CaSR, which is able to respond to physiological agonists by mobilizing Ca2+ from intracellular InsP3-sensitive stores. Facultad de Ciencias Exactas |
description |
The mechanism whereby extracellular Ca2+ exerts the endothelium-dependent control of vascular tone is still unclear. In this study, we assessed whether cardiac microvascular endothelial cells (CMEC) express a functional extracellular Ca2+-sensing receptor (CaSR) using a variety of techniques. CaSR mRNA was detected using RT-PCR, and CaSR protein was identified by immunocytochemical analysis. In order to assess the functionality of the receptor, CMEC were loaded with the Ca2+-sensitive fluorochrome, Fura-2/AM. A number of CaSR agonists, such as spermine, Gd 3+, La3+ and neomycin, elicited a heterogeneous intracellular Ca2+ signal, which was abolished by disruption of inositol 1,4,5-trisphosphate (InsP3) signaling and by depletion of intracellular stores with cyclopiazonic acid. The inhibition of the Na +/Ca2+ exchanger upon substitution of extracellular Na+ unmasked the Ca2+ signal triggered by an increase in extracellular Ca2+ levels. Finally, aromatic amino acids, which function as allosteric activators of CaSR, potentiated the Ca2+ response to the CaSR agonist La3+. These data provide evidence that CMEC express CaSR, which is able to respond to physiological agonists by mobilizing Ca2+ from intracellular InsP3-sensitive stores. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 |
dc.type.none.fl_str_mv |
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article |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/83531 |
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http://sedici.unlp.edu.ar/handle/10915/83531 |
dc.language.none.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/altIdentifier/issn/1018-1172 info:eu-repo/semantics/altIdentifier/doi/10.1159/000140677 |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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