Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor

Autores
Berra Romani, Roberto; Raqeeb, Abdul; Laforenza, Umberto; Scaffino, Manuela Federica; Moccia, Francesco; Avelino-Cruz, Josè Everardo; Oldani, Amanda; Coltrini, Daniela; Milesi, Verónica; Taglietti, Vanni; Tanzi, Franco
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The mechanism whereby extracellular Ca2+ exerts the endothelium-dependent control of vascular tone is still unclear. In this study, we assessed whether cardiac microvascular endothelial cells (CMEC) express a functional extracellular Ca2+-sensing receptor (CaSR) using a variety of techniques. CaSR mRNA was detected using RT-PCR, and CaSR protein was identified by immunocytochemical analysis. In order to assess the functionality of the receptor, CMEC were loaded with the Ca2+-sensitive fluorochrome, Fura-2/AM. A number of CaSR agonists, such as spermine, Gd 3+, La3+ and neomycin, elicited a heterogeneous intracellular Ca2+ signal, which was abolished by disruption of inositol 1,4,5-trisphosphate (InsP3) signaling and by depletion of intracellular stores with cyclopiazonic acid. The inhibition of the Na +/Ca2+ exchanger upon substitution of extracellular Na+ unmasked the Ca2+ signal triggered by an increase in extracellular Ca2+ levels. Finally, aromatic amino acids, which function as allosteric activators of CaSR, potentiated the Ca2+ response to the CaSR agonist La3+. These data provide evidence that CMEC express CaSR, which is able to respond to physiological agonists by mobilizing Ca2+ from intracellular InsP3-sensitive stores.
Facultad de Ciencias Exactas
Materia
Química
Ca2+-sensing receptor
Cardiac function
Cardiac microvascular endothelial cells
Extracellular Ca2+
Fura-2/AM
Inositol 1,4,5-trisphosphate
Na+/Ca2+ exchanger
Phospholipase C
Vascular tone
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/83531

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/83531
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptorBerra Romani, RobertoRaqeeb, AbdulLaforenza, UmbertoScaffino, Manuela FedericaMoccia, FrancescoAvelino-Cruz, Josè EverardoOldani, AmandaColtrini, DanielaMilesi, VerónicaTaglietti, VanniTanzi, FrancoQuímicaCa2+-sensing receptorCardiac functionCardiac microvascular endothelial cellsExtracellular Ca2+Fura-2/AMInositol 1,4,5-trisphosphateNa+/Ca2+ exchangerPhospholipase CVascular toneThe mechanism whereby extracellular Ca2+ exerts the endothelium-dependent control of vascular tone is still unclear. In this study, we assessed whether cardiac microvascular endothelial cells (CMEC) express a functional extracellular Ca2+-sensing receptor (CaSR) using a variety of techniques. CaSR mRNA was detected using RT-PCR, and CaSR protein was identified by immunocytochemical analysis. In order to assess the functionality of the receptor, CMEC were loaded with the Ca2+-sensitive fluorochrome, Fura-2/AM. A number of CaSR agonists, such as spermine, Gd 3+, La3+ and neomycin, elicited a heterogeneous intracellular Ca2+ signal, which was abolished by disruption of inositol 1,4,5-trisphosphate (InsP3) signaling and by depletion of intracellular stores with cyclopiazonic acid. The inhibition of the Na +/Ca2+ exchanger upon substitution of extracellular Na+ unmasked the Ca2+ signal triggered by an increase in extracellular Ca2+ levels. Finally, aromatic amino acids, which function as allosteric activators of CaSR, potentiated the Ca2+ response to the CaSR agonist La3+. These data provide evidence that CMEC express CaSR, which is able to respond to physiological agonists by mobilizing Ca2+ from intracellular InsP3-sensitive stores.Facultad de Ciencias Exactas2008info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf73-82http://sedici.unlp.edu.ar/handle/10915/83531enginfo:eu-repo/semantics/altIdentifier/issn/1018-1172info:eu-repo/semantics/altIdentifier/doi/10.1159/000140677info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:15:40Zoai:sedici.unlp.edu.ar:10915/83531Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:15:41.234SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
title Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
spellingShingle Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
Berra Romani, Roberto
Química
Ca2+-sensing receptor
Cardiac function
Cardiac microvascular endothelial cells
Extracellular Ca2+
Fura-2/AM
Inositol 1,4,5-trisphosphate
Na+/Ca2+ exchanger
Phospholipase C
Vascular tone
title_short Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
title_full Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
title_fullStr Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
title_full_unstemmed Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
title_sort Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
dc.creator.none.fl_str_mv Berra Romani, Roberto
Raqeeb, Abdul
Laforenza, Umberto
Scaffino, Manuela Federica
Moccia, Francesco
Avelino-Cruz, Josè Everardo
Oldani, Amanda
Coltrini, Daniela
Milesi, Verónica
Taglietti, Vanni
Tanzi, Franco
author Berra Romani, Roberto
author_facet Berra Romani, Roberto
Raqeeb, Abdul
Laforenza, Umberto
Scaffino, Manuela Federica
Moccia, Francesco
Avelino-Cruz, Josè Everardo
Oldani, Amanda
Coltrini, Daniela
Milesi, Verónica
Taglietti, Vanni
Tanzi, Franco
author_role author
author2 Raqeeb, Abdul
Laforenza, Umberto
Scaffino, Manuela Federica
Moccia, Francesco
Avelino-Cruz, Josè Everardo
Oldani, Amanda
Coltrini, Daniela
Milesi, Verónica
Taglietti, Vanni
Tanzi, Franco
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Química
Ca2+-sensing receptor
Cardiac function
Cardiac microvascular endothelial cells
Extracellular Ca2+
Fura-2/AM
Inositol 1,4,5-trisphosphate
Na+/Ca2+ exchanger
Phospholipase C
Vascular tone
topic Química
Ca2+-sensing receptor
Cardiac function
Cardiac microvascular endothelial cells
Extracellular Ca2+
Fura-2/AM
Inositol 1,4,5-trisphosphate
Na+/Ca2+ exchanger
Phospholipase C
Vascular tone
dc.description.none.fl_txt_mv The mechanism whereby extracellular Ca2+ exerts the endothelium-dependent control of vascular tone is still unclear. In this study, we assessed whether cardiac microvascular endothelial cells (CMEC) express a functional extracellular Ca2+-sensing receptor (CaSR) using a variety of techniques. CaSR mRNA was detected using RT-PCR, and CaSR protein was identified by immunocytochemical analysis. In order to assess the functionality of the receptor, CMEC were loaded with the Ca2+-sensitive fluorochrome, Fura-2/AM. A number of CaSR agonists, such as spermine, Gd 3+, La3+ and neomycin, elicited a heterogeneous intracellular Ca2+ signal, which was abolished by disruption of inositol 1,4,5-trisphosphate (InsP3) signaling and by depletion of intracellular stores with cyclopiazonic acid. The inhibition of the Na +/Ca2+ exchanger upon substitution of extracellular Na+ unmasked the Ca2+ signal triggered by an increase in extracellular Ca2+ levels. Finally, aromatic amino acids, which function as allosteric activators of CaSR, potentiated the Ca2+ response to the CaSR agonist La3+. These data provide evidence that CMEC express CaSR, which is able to respond to physiological agonists by mobilizing Ca2+ from intracellular InsP3-sensitive stores.
Facultad de Ciencias Exactas
description The mechanism whereby extracellular Ca2+ exerts the endothelium-dependent control of vascular tone is still unclear. In this study, we assessed whether cardiac microvascular endothelial cells (CMEC) express a functional extracellular Ca2+-sensing receptor (CaSR) using a variety of techniques. CaSR mRNA was detected using RT-PCR, and CaSR protein was identified by immunocytochemical analysis. In order to assess the functionality of the receptor, CMEC were loaded with the Ca2+-sensitive fluorochrome, Fura-2/AM. A number of CaSR agonists, such as spermine, Gd 3+, La3+ and neomycin, elicited a heterogeneous intracellular Ca2+ signal, which was abolished by disruption of inositol 1,4,5-trisphosphate (InsP3) signaling and by depletion of intracellular stores with cyclopiazonic acid. The inhibition of the Na +/Ca2+ exchanger upon substitution of extracellular Na+ unmasked the Ca2+ signal triggered by an increase in extracellular Ca2+ levels. Finally, aromatic amino acids, which function as allosteric activators of CaSR, potentiated the Ca2+ response to the CaSR agonist La3+. These data provide evidence that CMEC express CaSR, which is able to respond to physiological agonists by mobilizing Ca2+ from intracellular InsP3-sensitive stores.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/83531
url http://sedici.unlp.edu.ar/handle/10915/83531
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1018-1172
info:eu-repo/semantics/altIdentifier/doi/10.1159/000140677
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
73-82
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instname:Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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