Colonization of sorghum and wheat by seed inoculation with <i>Gluconacetobacter diazotrophicus</i>

Autores
Luna, María Flavia; Galar, María Lina; Aprea, Julieta; Molinari, María Laura; Boiardi, José Luis
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Colonization of sorghum and wheat after seed inoculation with Gluconacetobacter diazotrophicus strains PAL 5 and UAP 5541/pRGS561 (containing the marker gene gusA) was studied by colony counting and microscopic observation of plant tissues. Inoculum levels as low as 10² CFU per seed were enough for root colonization and further spreading in aerial tissues. Rhizoplane colonization was around 7 log CFU g⁻¹ (fresh weight). G. diazotrophicus was found inside sorghum and wheat roots with populations higher than 5 log CFU g⁻¹ (fresh weight). Stem colonization remained stable for 30 days post inoculation with endophyte concentrations from 4 to 5 log CFU g⁻¹ (fresh weight) (in both plants). Population in leaves decreased continuously being undetectable after 17 days post inoculation.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
Química
Endophytes
Gluconacetobacter diazotrophicus
Inoculation
Sorghum
Wheat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/144416

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network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Colonization of sorghum and wheat by seed inoculation with <i>Gluconacetobacter diazotrophicus</i>Luna, María FlaviaGalar, María LinaAprea, JulietaMolinari, María LauraBoiardi, José LuisCiencias ExactasQuímicaEndophytesGluconacetobacter diazotrophicusInoculationSorghumWheatColonization of sorghum and wheat after seed inoculation with <i>Gluconacetobacter diazotrophicus</i> strains PAL 5 and UAP 5541/pRGS561 (containing the marker gene gusA) was studied by colony counting and microscopic observation of plant tissues. Inoculum levels as low as 10² CFU per seed were enough for root colonization and further spreading in aerial tissues. Rhizoplane colonization was around 7 log CFU g⁻¹ (fresh weight). <i>G. diazotrophicus</i> was found inside sorghum and wheat roots with populations higher than 5 log CFU g⁻¹ (fresh weight). Stem colonization remained stable for 30 days post inoculation with endophyte concentrations from 4 to 5 log CFU g⁻¹ (fresh weight) (in both plants). Population in leaves decreased continuously being undetectable after 17 days post inoculation.Centro de Investigación y Desarrollo en Fermentaciones Industriales2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1071-1076http://sedici.unlp.edu.ar/handle/10915/144416enginfo:eu-repo/semantics/altIdentifier/issn/1573-6776info:eu-repo/semantics/altIdentifier/issn/0141-5492info:eu-repo/semantics/altIdentifier/doi/10.1007/s10529-010-0256-2info:eu-repo/semantics/altIdentifier/pmid/20361236info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:27Zoai:sedici.unlp.edu.ar:10915/144416Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:28.127SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Colonization of sorghum and wheat by seed inoculation with <i>Gluconacetobacter diazotrophicus</i>
title Colonization of sorghum and wheat by seed inoculation with <i>Gluconacetobacter diazotrophicus</i>
spellingShingle Colonization of sorghum and wheat by seed inoculation with <i>Gluconacetobacter diazotrophicus</i>
Luna, María Flavia
Ciencias Exactas
Química
Endophytes
Gluconacetobacter diazotrophicus
Inoculation
Sorghum
Wheat
title_short Colonization of sorghum and wheat by seed inoculation with <i>Gluconacetobacter diazotrophicus</i>
title_full Colonization of sorghum and wheat by seed inoculation with <i>Gluconacetobacter diazotrophicus</i>
title_fullStr Colonization of sorghum and wheat by seed inoculation with <i>Gluconacetobacter diazotrophicus</i>
title_full_unstemmed Colonization of sorghum and wheat by seed inoculation with <i>Gluconacetobacter diazotrophicus</i>
title_sort Colonization of sorghum and wheat by seed inoculation with <i>Gluconacetobacter diazotrophicus</i>
dc.creator.none.fl_str_mv Luna, María Flavia
Galar, María Lina
Aprea, Julieta
Molinari, María Laura
Boiardi, José Luis
author Luna, María Flavia
author_facet Luna, María Flavia
Galar, María Lina
Aprea, Julieta
Molinari, María Laura
Boiardi, José Luis
author_role author
author2 Galar, María Lina
Aprea, Julieta
Molinari, María Laura
Boiardi, José Luis
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Química
Endophytes
Gluconacetobacter diazotrophicus
Inoculation
Sorghum
Wheat
topic Ciencias Exactas
Química
Endophytes
Gluconacetobacter diazotrophicus
Inoculation
Sorghum
Wheat
dc.description.none.fl_txt_mv Colonization of sorghum and wheat after seed inoculation with <i>Gluconacetobacter diazotrophicus</i> strains PAL 5 and UAP 5541/pRGS561 (containing the marker gene gusA) was studied by colony counting and microscopic observation of plant tissues. Inoculum levels as low as 10² CFU per seed were enough for root colonization and further spreading in aerial tissues. Rhizoplane colonization was around 7 log CFU g⁻¹ (fresh weight). <i>G. diazotrophicus</i> was found inside sorghum and wheat roots with populations higher than 5 log CFU g⁻¹ (fresh weight). Stem colonization remained stable for 30 days post inoculation with endophyte concentrations from 4 to 5 log CFU g⁻¹ (fresh weight) (in both plants). Population in leaves decreased continuously being undetectable after 17 days post inoculation.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description Colonization of sorghum and wheat after seed inoculation with <i>Gluconacetobacter diazotrophicus</i> strains PAL 5 and UAP 5541/pRGS561 (containing the marker gene gusA) was studied by colony counting and microscopic observation of plant tissues. Inoculum levels as low as 10² CFU per seed were enough for root colonization and further spreading in aerial tissues. Rhizoplane colonization was around 7 log CFU g⁻¹ (fresh weight). <i>G. diazotrophicus</i> was found inside sorghum and wheat roots with populations higher than 5 log CFU g⁻¹ (fresh weight). Stem colonization remained stable for 30 days post inoculation with endophyte concentrations from 4 to 5 log CFU g⁻¹ (fresh weight) (in both plants). Population in leaves decreased continuously being undetectable after 17 days post inoculation.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/144416
url http://sedici.unlp.edu.ar/handle/10915/144416
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1573-6776
info:eu-repo/semantics/altIdentifier/issn/0141-5492
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10529-010-0256-2
info:eu-repo/semantics/altIdentifier/pmid/20361236
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
1071-1076
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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