Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia

Autores
Del Papa, María Florencia; Pistorio, Mariano; Draghi, Walter Omar; Lozano, Mauricio Javier; Giusti, María de los Ángeles; Medina, C.; Van Dillewijn, P.; Martínez-Abarca, F.; Flores, B.M.; Ruiz-Sainz, J.E.; Megías, M.; Pühler, A.; Niehaus, K.; Toro, N.; Lagares, Antonio
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nodulation of Medicago sativa (alfalfa) is known to be restricted to Sinorhizobium meliloti and a few other rhizobia that include the poorly characterized isolates related to Rhizobium sp. strain Or191. Distinctive features of the symbiosis between alfalfa and S. meliloti are the marked specificity from the plant to the bacteria and the strict requirement for the presence of sulfated lipochitooligosaccharides (Nod factors [NFs]) at its reducing end. Here, we present evidence of the presence of a functional nodH-encoded NF sulfotransferase in the Or191-like rhizobia. The nodH gene, present in single copy, maps to a high molecular weight megaplasmid. As in S. meliloti, a nodF homolog was identified immediately upstream of nodH that was transcribed in the opposite direction (local synteny). This novel nodH ortholog was cloned and shown to restore both NF sulfation and the Nif +Fix+ phenotypes when introduced into an S. meliloti nodH mutant. Unexpectedly, however, nodH disruption in the Or191-like bacteria did not abolish their ability to nodulate alfalfa, resulting instead in a severely delayed nodulation. In agreement with evidence from other authors, the nodH sequence analysis strongly supports the idea that the Or191-like rhizobia most likely represent a genetic mosaic resulting from the horizontal transfer of symbiotic genes from a sinorhizobial megaplasmid to a not yet clearly identified ancestor.
Instituto de Biotecnologia y Biologia Molecular
Materia
Bioquímica
Sinorhizobium meliloti
Medicago sativa
nodH gene
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/83078

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spelling Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobiaDel Papa, María FlorenciaPistorio, MarianoDraghi, Walter OmarLozano, Mauricio JavierGiusti, María de los ÁngelesMedina, C.Van Dillewijn, P.Martínez-Abarca, F.Flores, B.M.Ruiz-Sainz, J.E.Megías, M.Pühler, A.Niehaus, K.Toro, N.Lagares, AntonioBioquímicaSinorhizobium melilotiMedicago sativanodH geneNodulation of Medicago sativa (alfalfa) is known to be restricted to Sinorhizobium meliloti and a few other rhizobia that include the poorly characterized isolates related to Rhizobium sp. strain Or191. Distinctive features of the symbiosis between alfalfa and S. meliloti are the marked specificity from the plant to the bacteria and the strict requirement for the presence of sulfated lipochitooligosaccharides (Nod factors [NFs]) at its reducing end. Here, we present evidence of the presence of a functional nodH-encoded NF sulfotransferase in the Or191-like rhizobia. The nodH gene, present in single copy, maps to a high molecular weight megaplasmid. As in S. meliloti, a nodF homolog was identified immediately upstream of nodH that was transcribed in the opposite direction (local synteny). This novel nodH ortholog was cloned and shown to restore both NF sulfation and the Nif +Fix+ phenotypes when introduced into an S. meliloti nodH mutant. Unexpectedly, however, nodH disruption in the Or191-like bacteria did not abolish their ability to nodulate alfalfa, resulting instead in a severely delayed nodulation. In agreement with evidence from other authors, the nodH sequence analysis strongly supports the idea that the Or191-like rhizobia most likely represent a genetic mosaic resulting from the horizontal transfer of symbiotic genes from a sinorhizobial megaplasmid to a not yet clearly identified ancestor.Instituto de Biotecnologia y Biologia Molecular2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf138-145http://sedici.unlp.edu.ar/handle/10915/83078enginfo:eu-repo/semantics/altIdentifier/issn/0894-0282info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-20-2-0138info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:07:38Zoai:sedici.unlp.edu.ar:10915/83078Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:07:38.488SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia
title Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia
spellingShingle Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia
Del Papa, María Florencia
Bioquímica
Sinorhizobium meliloti
Medicago sativa
nodH gene
title_short Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia
title_full Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia
title_fullStr Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia
title_full_unstemmed Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia
title_sort Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia
dc.creator.none.fl_str_mv Del Papa, María Florencia
Pistorio, Mariano
Draghi, Walter Omar
Lozano, Mauricio Javier
Giusti, María de los Ángeles
Medina, C.
Van Dillewijn, P.
Martínez-Abarca, F.
Flores, B.M.
Ruiz-Sainz, J.E.
Megías, M.
Pühler, A.
Niehaus, K.
Toro, N.
Lagares, Antonio
author Del Papa, María Florencia
author_facet Del Papa, María Florencia
Pistorio, Mariano
Draghi, Walter Omar
Lozano, Mauricio Javier
Giusti, María de los Ángeles
Medina, C.
Van Dillewijn, P.
Martínez-Abarca, F.
Flores, B.M.
Ruiz-Sainz, J.E.
Megías, M.
Pühler, A.
Niehaus, K.
Toro, N.
Lagares, Antonio
author_role author
author2 Pistorio, Mariano
Draghi, Walter Omar
Lozano, Mauricio Javier
Giusti, María de los Ángeles
Medina, C.
Van Dillewijn, P.
Martínez-Abarca, F.
Flores, B.M.
Ruiz-Sainz, J.E.
Megías, M.
Pühler, A.
Niehaus, K.
Toro, N.
Lagares, Antonio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
Sinorhizobium meliloti
Medicago sativa
nodH gene
topic Bioquímica
Sinorhizobium meliloti
Medicago sativa
nodH gene
dc.description.none.fl_txt_mv Nodulation of Medicago sativa (alfalfa) is known to be restricted to Sinorhizobium meliloti and a few other rhizobia that include the poorly characterized isolates related to Rhizobium sp. strain Or191. Distinctive features of the symbiosis between alfalfa and S. meliloti are the marked specificity from the plant to the bacteria and the strict requirement for the presence of sulfated lipochitooligosaccharides (Nod factors [NFs]) at its reducing end. Here, we present evidence of the presence of a functional nodH-encoded NF sulfotransferase in the Or191-like rhizobia. The nodH gene, present in single copy, maps to a high molecular weight megaplasmid. As in S. meliloti, a nodF homolog was identified immediately upstream of nodH that was transcribed in the opposite direction (local synteny). This novel nodH ortholog was cloned and shown to restore both NF sulfation and the Nif +Fix+ phenotypes when introduced into an S. meliloti nodH mutant. Unexpectedly, however, nodH disruption in the Or191-like bacteria did not abolish their ability to nodulate alfalfa, resulting instead in a severely delayed nodulation. In agreement with evidence from other authors, the nodH sequence analysis strongly supports the idea that the Or191-like rhizobia most likely represent a genetic mosaic resulting from the horizontal transfer of symbiotic genes from a sinorhizobial megaplasmid to a not yet clearly identified ancestor.
Instituto de Biotecnologia y Biologia Molecular
description Nodulation of Medicago sativa (alfalfa) is known to be restricted to Sinorhizobium meliloti and a few other rhizobia that include the poorly characterized isolates related to Rhizobium sp. strain Or191. Distinctive features of the symbiosis between alfalfa and S. meliloti are the marked specificity from the plant to the bacteria and the strict requirement for the presence of sulfated lipochitooligosaccharides (Nod factors [NFs]) at its reducing end. Here, we present evidence of the presence of a functional nodH-encoded NF sulfotransferase in the Or191-like rhizobia. The nodH gene, present in single copy, maps to a high molecular weight megaplasmid. As in S. meliloti, a nodF homolog was identified immediately upstream of nodH that was transcribed in the opposite direction (local synteny). This novel nodH ortholog was cloned and shown to restore both NF sulfation and the Nif +Fix+ phenotypes when introduced into an S. meliloti nodH mutant. Unexpectedly, however, nodH disruption in the Or191-like bacteria did not abolish their ability to nodulate alfalfa, resulting instead in a severely delayed nodulation. In agreement with evidence from other authors, the nodH sequence analysis strongly supports the idea that the Or191-like rhizobia most likely represent a genetic mosaic resulting from the horizontal transfer of symbiotic genes from a sinorhizobial megaplasmid to a not yet clearly identified ancestor.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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format article
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info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-20-2-0138
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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