Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia
- Autores
- Del Papa, María Florencia; Pistorio, Mariano; Draghi, Walter Omar; Lozano, Mauricio Javier; Giusti, María de los Ángeles; Medina, C.; Van Dillewijn, P.; Martínez-Abarca, F.; Flores, B.M.; Ruiz-Sainz, J.E.; Megías, M.; Pühler, A.; Niehaus, K.; Toro, N.; Lagares, Antonio
- Año de publicación
- 2007
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Nodulation of Medicago sativa (alfalfa) is known to be restricted to Sinorhizobium meliloti and a few other rhizobia that include the poorly characterized isolates related to Rhizobium sp. strain Or191. Distinctive features of the symbiosis between alfalfa and S. meliloti are the marked specificity from the plant to the bacteria and the strict requirement for the presence of sulfated lipochitooligosaccharides (Nod factors [NFs]) at its reducing end. Here, we present evidence of the presence of a functional nodH-encoded NF sulfotransferase in the Or191-like rhizobia. The nodH gene, present in single copy, maps to a high molecular weight megaplasmid. As in S. meliloti, a nodF homolog was identified immediately upstream of nodH that was transcribed in the opposite direction (local synteny). This novel nodH ortholog was cloned and shown to restore both NF sulfation and the Nif +Fix+ phenotypes when introduced into an S. meliloti nodH mutant. Unexpectedly, however, nodH disruption in the Or191-like bacteria did not abolish their ability to nodulate alfalfa, resulting instead in a severely delayed nodulation. In agreement with evidence from other authors, the nodH sequence analysis strongly supports the idea that the Or191-like rhizobia most likely represent a genetic mosaic resulting from the horizontal transfer of symbiotic genes from a sinorhizobial megaplasmid to a not yet clearly identified ancestor.
Instituto de Biotecnologia y Biologia Molecular - Materia
-
Bioquímica
Sinorhizobium meliloti
Medicago sativa
nodH gene - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/83078
Ver los metadatos del registro completo
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Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobiaDel Papa, María FlorenciaPistorio, MarianoDraghi, Walter OmarLozano, Mauricio JavierGiusti, María de los ÁngelesMedina, C.Van Dillewijn, P.Martínez-Abarca, F.Flores, B.M.Ruiz-Sainz, J.E.Megías, M.Pühler, A.Niehaus, K.Toro, N.Lagares, AntonioBioquímicaSinorhizobium melilotiMedicago sativanodH geneNodulation of Medicago sativa (alfalfa) is known to be restricted to Sinorhizobium meliloti and a few other rhizobia that include the poorly characterized isolates related to Rhizobium sp. strain Or191. Distinctive features of the symbiosis between alfalfa and S. meliloti are the marked specificity from the plant to the bacteria and the strict requirement for the presence of sulfated lipochitooligosaccharides (Nod factors [NFs]) at its reducing end. Here, we present evidence of the presence of a functional nodH-encoded NF sulfotransferase in the Or191-like rhizobia. The nodH gene, present in single copy, maps to a high molecular weight megaplasmid. As in S. meliloti, a nodF homolog was identified immediately upstream of nodH that was transcribed in the opposite direction (local synteny). This novel nodH ortholog was cloned and shown to restore both NF sulfation and the Nif +Fix+ phenotypes when introduced into an S. meliloti nodH mutant. Unexpectedly, however, nodH disruption in the Or191-like bacteria did not abolish their ability to nodulate alfalfa, resulting instead in a severely delayed nodulation. In agreement with evidence from other authors, the nodH sequence analysis strongly supports the idea that the Or191-like rhizobia most likely represent a genetic mosaic resulting from the horizontal transfer of symbiotic genes from a sinorhizobial megaplasmid to a not yet clearly identified ancestor.Instituto de Biotecnologia y Biologia Molecular2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf138-145http://sedici.unlp.edu.ar/handle/10915/83078enginfo:eu-repo/semantics/altIdentifier/issn/0894-0282info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-20-2-0138info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:07:38Zoai:sedici.unlp.edu.ar:10915/83078Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:07:38.488SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia |
title |
Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia |
spellingShingle |
Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia Del Papa, María Florencia Bioquímica Sinorhizobium meliloti Medicago sativa nodH gene |
title_short |
Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia |
title_full |
Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia |
title_fullStr |
Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia |
title_full_unstemmed |
Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia |
title_sort |
Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia |
dc.creator.none.fl_str_mv |
Del Papa, María Florencia Pistorio, Mariano Draghi, Walter Omar Lozano, Mauricio Javier Giusti, María de los Ángeles Medina, C. Van Dillewijn, P. Martínez-Abarca, F. Flores, B.M. Ruiz-Sainz, J.E. Megías, M. Pühler, A. Niehaus, K. Toro, N. Lagares, Antonio |
author |
Del Papa, María Florencia |
author_facet |
Del Papa, María Florencia Pistorio, Mariano Draghi, Walter Omar Lozano, Mauricio Javier Giusti, María de los Ángeles Medina, C. Van Dillewijn, P. Martínez-Abarca, F. Flores, B.M. Ruiz-Sainz, J.E. Megías, M. Pühler, A. Niehaus, K. Toro, N. Lagares, Antonio |
author_role |
author |
author2 |
Pistorio, Mariano Draghi, Walter Omar Lozano, Mauricio Javier Giusti, María de los Ángeles Medina, C. Van Dillewijn, P. Martínez-Abarca, F. Flores, B.M. Ruiz-Sainz, J.E. Megías, M. Pühler, A. Niehaus, K. Toro, N. Lagares, Antonio |
author2_role |
author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Bioquímica Sinorhizobium meliloti Medicago sativa nodH gene |
topic |
Bioquímica Sinorhizobium meliloti Medicago sativa nodH gene |
dc.description.none.fl_txt_mv |
Nodulation of Medicago sativa (alfalfa) is known to be restricted to Sinorhizobium meliloti and a few other rhizobia that include the poorly characterized isolates related to Rhizobium sp. strain Or191. Distinctive features of the symbiosis between alfalfa and S. meliloti are the marked specificity from the plant to the bacteria and the strict requirement for the presence of sulfated lipochitooligosaccharides (Nod factors [NFs]) at its reducing end. Here, we present evidence of the presence of a functional nodH-encoded NF sulfotransferase in the Or191-like rhizobia. The nodH gene, present in single copy, maps to a high molecular weight megaplasmid. As in S. meliloti, a nodF homolog was identified immediately upstream of nodH that was transcribed in the opposite direction (local synteny). This novel nodH ortholog was cloned and shown to restore both NF sulfation and the Nif +Fix+ phenotypes when introduced into an S. meliloti nodH mutant. Unexpectedly, however, nodH disruption in the Or191-like bacteria did not abolish their ability to nodulate alfalfa, resulting instead in a severely delayed nodulation. In agreement with evidence from other authors, the nodH sequence analysis strongly supports the idea that the Or191-like rhizobia most likely represent a genetic mosaic resulting from the horizontal transfer of symbiotic genes from a sinorhizobial megaplasmid to a not yet clearly identified ancestor. Instituto de Biotecnologia y Biologia Molecular |
description |
Nodulation of Medicago sativa (alfalfa) is known to be restricted to Sinorhizobium meliloti and a few other rhizobia that include the poorly characterized isolates related to Rhizobium sp. strain Or191. Distinctive features of the symbiosis between alfalfa and S. meliloti are the marked specificity from the plant to the bacteria and the strict requirement for the presence of sulfated lipochitooligosaccharides (Nod factors [NFs]) at its reducing end. Here, we present evidence of the presence of a functional nodH-encoded NF sulfotransferase in the Or191-like rhizobia. The nodH gene, present in single copy, maps to a high molecular weight megaplasmid. As in S. meliloti, a nodF homolog was identified immediately upstream of nodH that was transcribed in the opposite direction (local synteny). This novel nodH ortholog was cloned and shown to restore both NF sulfation and the Nif +Fix+ phenotypes when introduced into an S. meliloti nodH mutant. Unexpectedly, however, nodH disruption in the Or191-like bacteria did not abolish their ability to nodulate alfalfa, resulting instead in a severely delayed nodulation. In agreement with evidence from other authors, the nodH sequence analysis strongly supports the idea that the Or191-like rhizobia most likely represent a genetic mosaic resulting from the horizontal transfer of symbiotic genes from a sinorhizobial megaplasmid to a not yet clearly identified ancestor. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 |
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format |
article |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/83078 |
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http://sedici.unlp.edu.ar/handle/10915/83078 |
dc.language.none.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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