In Vitro Activity of Neem (<i>Azadirachta indica</i>) Oil on Growth and Ochratoxin A Production by <i>Aspergillus carbonarius</i> Isolates

Autores
Rodrigues, Mariana Paiva; Astoreca, Andrea Luciana; Oliveira, Águida Aparecida de; Salvato, Lauranne Alves; Lago Biscoto, Gabriela; Moura Keller, Luiz Antonio; Rocha Rosa, Carlos Alberto da; Cavaglieri, Lilia Renée; Azevedo, Maria Isabel de; Moura Keller, Kelly
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aspergillus carbonarius is a saprobic filamentous fungus, food spoiling fungus and a producer of ochratoxin A (OTA) mycotoxin. In this study, the in vitro antifungal activity of neem oil (0.12% p/p of azadirachtin) was evaluated against the growth of six strains of A. carbonarius and the production of OTA. Four different concentrations of neem oil were tested in addition to three incubation times. Only the concentration of 0.3% of neem oil inhibited more than 95% of the strain’s growth (97.6% ± 0.5%), while the use of 0.5% and 1.0% of neem oil showed lower antifungal activity, 40.2% ± 3.1 and 64.7% ± 1.1, respectively. There was a complete inhibition of OTA production with 0.1% and 0.3% neem oil in the four strains isolated in the laboratory from grapes. The present study shows that neem essential oil can be further evaluated as an auxiliary method for the reduction of mycelial growth and OTA production.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
mycotoxins
essential oils
ecophysiology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/106711
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/106711
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling In Vitro Activity of Neem (<i>Azadirachta indica</i>) Oil on Growth and Ochratoxin A Production by <i>Aspergillus carbonarius</i> IsolatesRodrigues, Mariana PaivaAstoreca, Andrea LucianaOliveira, Águida Aparecida deSalvato, Lauranne AlvesLago Biscoto, GabrielaMoura Keller, Luiz AntonioRocha Rosa, Carlos Alberto daCavaglieri, Lilia RenéeAzevedo, Maria Isabel deMoura Keller, KellyCiencias Exactasmycotoxinsessential oilsecophysiology<i>Aspergillus carbonarius</i> is a saprobic filamentous fungus, food spoiling fungus and a producer of ochratoxin A (OTA) mycotoxin. In this study, the in vitro antifungal activity of neem oil (0.12% p/p of azadirachtin) was evaluated against the growth of six strains of <i>A. carbonarius</i> and the production of OTA. Four different concentrations of neem oil were tested in addition to three incubation times. Only the concentration of 0.3% of neem oil inhibited more than 95% of the strain’s growth (97.6% ± 0.5%), while the use of 0.5% and 1.0% of neem oil showed lower antifungal activity, 40.2% ± 3.1 and 64.7% ± 1.1, respectively. There was a complete inhibition of OTA production with 0.1% and 0.3% neem oil in the four strains isolated in the laboratory from grapes. The present study shows that neem essential oil can be further evaluated as an auxiliary method for the reduction of mycelial growth and OTA production.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriales2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/106711enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6833479&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6651/11/10/579info:eu-repo/semantics/altIdentifier/issn/2072-6651info:eu-repo/semantics/altIdentifier/pmid/31590398info:eu-repo/semantics/altIdentifier/doi/10.3390/toxins11100579info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:23:52Zoai:sedici.unlp.edu.ar:10915/106711Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:23:53.052SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv In Vitro Activity of Neem (<i>Azadirachta indica</i>) Oil on Growth and Ochratoxin A Production by <i>Aspergillus carbonarius</i> Isolates
title In Vitro Activity of Neem (<i>Azadirachta indica</i>) Oil on Growth and Ochratoxin A Production by <i>Aspergillus carbonarius</i> Isolates
spellingShingle In Vitro Activity of Neem (<i>Azadirachta indica</i>) Oil on Growth and Ochratoxin A Production by <i>Aspergillus carbonarius</i> Isolates
Rodrigues, Mariana Paiva
Ciencias Exactas
mycotoxins
essential oils
ecophysiology
title_short In Vitro Activity of Neem (<i>Azadirachta indica</i>) Oil on Growth and Ochratoxin A Production by <i>Aspergillus carbonarius</i> Isolates
title_full In Vitro Activity of Neem (<i>Azadirachta indica</i>) Oil on Growth and Ochratoxin A Production by <i>Aspergillus carbonarius</i> Isolates
title_fullStr In Vitro Activity of Neem (<i>Azadirachta indica</i>) Oil on Growth and Ochratoxin A Production by <i>Aspergillus carbonarius</i> Isolates
title_full_unstemmed In Vitro Activity of Neem (<i>Azadirachta indica</i>) Oil on Growth and Ochratoxin A Production by <i>Aspergillus carbonarius</i> Isolates
title_sort In Vitro Activity of Neem (<i>Azadirachta indica</i>) Oil on Growth and Ochratoxin A Production by <i>Aspergillus carbonarius</i> Isolates
dc.creator.none.fl_str_mv Rodrigues, Mariana Paiva
Astoreca, Andrea Luciana
Oliveira, Águida Aparecida de
Salvato, Lauranne Alves
Lago Biscoto, Gabriela
Moura Keller, Luiz Antonio
Rocha Rosa, Carlos Alberto da
Cavaglieri, Lilia Renée
Azevedo, Maria Isabel de
Moura Keller, Kelly
author Rodrigues, Mariana Paiva
author_facet Rodrigues, Mariana Paiva
Astoreca, Andrea Luciana
Oliveira, Águida Aparecida de
Salvato, Lauranne Alves
Lago Biscoto, Gabriela
Moura Keller, Luiz Antonio
Rocha Rosa, Carlos Alberto da
Cavaglieri, Lilia Renée
Azevedo, Maria Isabel de
Moura Keller, Kelly
author_role author
author2 Astoreca, Andrea Luciana
Oliveira, Águida Aparecida de
Salvato, Lauranne Alves
Lago Biscoto, Gabriela
Moura Keller, Luiz Antonio
Rocha Rosa, Carlos Alberto da
Cavaglieri, Lilia Renée
Azevedo, Maria Isabel de
Moura Keller, Kelly
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
mycotoxins
essential oils
ecophysiology
topic Ciencias Exactas
mycotoxins
essential oils
ecophysiology
dc.description.none.fl_txt_mv <i>Aspergillus carbonarius</i> is a saprobic filamentous fungus, food spoiling fungus and a producer of ochratoxin A (OTA) mycotoxin. In this study, the in vitro antifungal activity of neem oil (0.12% p/p of azadirachtin) was evaluated against the growth of six strains of <i>A. carbonarius</i> and the production of OTA. Four different concentrations of neem oil were tested in addition to three incubation times. Only the concentration of 0.3% of neem oil inhibited more than 95% of the strain’s growth (97.6% ± 0.5%), while the use of 0.5% and 1.0% of neem oil showed lower antifungal activity, 40.2% ± 3.1 and 64.7% ± 1.1, respectively. There was a complete inhibition of OTA production with 0.1% and 0.3% neem oil in the four strains isolated in the laboratory from grapes. The present study shows that neem essential oil can be further evaluated as an auxiliary method for the reduction of mycelial growth and OTA production.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description <i>Aspergillus carbonarius</i> is a saprobic filamentous fungus, food spoiling fungus and a producer of ochratoxin A (OTA) mycotoxin. In this study, the in vitro antifungal activity of neem oil (0.12% p/p of azadirachtin) was evaluated against the growth of six strains of <i>A. carbonarius</i> and the production of OTA. Four different concentrations of neem oil were tested in addition to three incubation times. Only the concentration of 0.3% of neem oil inhibited more than 95% of the strain’s growth (97.6% ± 0.5%), while the use of 0.5% and 1.0% of neem oil showed lower antifungal activity, 40.2% ± 3.1 and 64.7% ± 1.1, respectively. There was a complete inhibition of OTA production with 0.1% and 0.3% neem oil in the four strains isolated in the laboratory from grapes. The present study shows that neem essential oil can be further evaluated as an auxiliary method for the reduction of mycelial growth and OTA production.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/106711
url http://sedici.unlp.edu.ar/handle/10915/106711
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6833479&blobtype=pdf
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6651/11/10/579
info:eu-repo/semantics/altIdentifier/issn/2072-6651
info:eu-repo/semantics/altIdentifier/pmid/31590398
info:eu-repo/semantics/altIdentifier/doi/10.3390/toxins11100579
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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