In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolates

Autores
Rodrigues, Mariana Paiva; Astoreca, Andrea Luciana; De Oliveira, Águida Aparecida; Salvato, Lauranne Alves; Biscoto, Gabriela Lago; Keller, Luiz Antonio Moura; Rosa, Carlos Alberto da Rocha; Cavaglieri, Lilia Reneé; De Azevedo, Maria Isabel; Keller, Kelly Moura
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aspergillus carbonarius is a saprobic filamentous fungus, food spoiling fungus and a producer of ochratoxin A (OTA) mycotoxin. In this study, the in vitro antifungal activity of neem oil (0.12% p/p of azadirachtin) was evaluated against the growth of six strains of A. carbonarius and the production of OTA. Four different concentrations of neem oil were tested in addition to three incubation times. Only the concentration of 0.3% of neem oil inhibited more than 95% of the strain’s growth (97.6% ± 0.5%), while the use of 0.5% and 1.0% of neem oil showed lower antifungal activity, 40.2% ± 3.1 and 64.7% ± 1.1, respectively. There was a complete inhibition of OTA production with 0.1% and 0.3% neem oil in the four strains isolated in the laboratory from grapes. The present study shows that neem essential oil can be further evaluated as an auxiliary method for the reduction of mycelial growth and OTA production.
Fil: Rodrigues, Mariana Paiva. Universidade Federal de Minas Gerais; Brasil
Fil: Astoreca, Andrea Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: De Oliveira, Águida Aparecida. Universidad Federal Rural de Rio de Janeiro; Brasil
Fil: Salvato, Lauranne Alves. Universidade Federal de Minas Gerais; Brasil
Fil: Biscoto, Gabriela Lago. Universidade Federal de Minas Gerais; Brasil
Fil: Keller, Luiz Antonio Moura. Universidade Federal Fluminense; Brasil
Fil: Rosa, Carlos Alberto da Rocha. Universidade Federal Do Rio de Janeiro. Instituto de Biología; Brasil
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales. Departamento de Quimica. Area Fisicoquimica Organica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: De Azevedo, Maria Isabel. Universidade Federal de Minas Gerais; Brasil
Fil: Keller, Kelly Moura. Universidade Federal de Minas Gerais; Brasil
Materia
ECOPHYSIOLOGY
ESSENTIAL OILS
MYCOTOXINS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/122752
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/122752
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolatesRodrigues, Mariana PaivaAstoreca, Andrea LucianaDe Oliveira, Águida AparecidaSalvato, Lauranne AlvesBiscoto, Gabriela LagoKeller, Luiz Antonio MouraRosa, Carlos Alberto da RochaCavaglieri, Lilia ReneéDe Azevedo, Maria IsabelKeller, Kelly MouraECOPHYSIOLOGYESSENTIAL OILSMYCOTOXINShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aspergillus carbonarius is a saprobic filamentous fungus, food spoiling fungus and a producer of ochratoxin A (OTA) mycotoxin. In this study, the in vitro antifungal activity of neem oil (0.12% p/p of azadirachtin) was evaluated against the growth of six strains of A. carbonarius and the production of OTA. Four different concentrations of neem oil were tested in addition to three incubation times. Only the concentration of 0.3% of neem oil inhibited more than 95% of the strain’s growth (97.6% ± 0.5%), while the use of 0.5% and 1.0% of neem oil showed lower antifungal activity, 40.2% ± 3.1 and 64.7% ± 1.1, respectively. There was a complete inhibition of OTA production with 0.1% and 0.3% neem oil in the four strains isolated in the laboratory from grapes. The present study shows that neem essential oil can be further evaluated as an auxiliary method for the reduction of mycelial growth and OTA production.Fil: Rodrigues, Mariana Paiva. Universidade Federal de Minas Gerais; BrasilFil: Astoreca, Andrea Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: De Oliveira, Águida Aparecida. Universidad Federal Rural de Rio de Janeiro; BrasilFil: Salvato, Lauranne Alves. Universidade Federal de Minas Gerais; BrasilFil: Biscoto, Gabriela Lago. Universidade Federal de Minas Gerais; BrasilFil: Keller, Luiz Antonio Moura. Universidade Federal Fluminense; BrasilFil: Rosa, Carlos Alberto da Rocha. Universidade Federal Do Rio de Janeiro. Instituto de Biología; BrasilFil: Cavaglieri, Lilia Reneé. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales. Departamento de Quimica. Area Fisicoquimica Organica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: De Azevedo, Maria Isabel. Universidade Federal de Minas Gerais; BrasilFil: Keller, Kelly Moura. Universidade Federal de Minas Gerais; BrasilMultidisciplinary Digital Publishing Institute2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/122752Rodrigues, Mariana Paiva; Astoreca, Andrea Luciana; De Oliveira, Águida Aparecida; Salvato, Lauranne Alves; Biscoto, Gabriela Lago; et al.; In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolates; Multidisciplinary Digital Publishing Institute; Toxins; 11; 10; 10-2019; 1-122072-6651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/toxins11100579info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6651/11/10/579info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:35:13Zoai:ri.conicet.gov.ar:11336/122752instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:35:13.324CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolates
title In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolates
spellingShingle In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolates
Rodrigues, Mariana Paiva
ECOPHYSIOLOGY
ESSENTIAL OILS
MYCOTOXINS
title_short In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolates
title_full In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolates
title_fullStr In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolates
title_full_unstemmed In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolates
title_sort In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolates
dc.creator.none.fl_str_mv Rodrigues, Mariana Paiva
Astoreca, Andrea Luciana
De Oliveira, Águida Aparecida
Salvato, Lauranne Alves
Biscoto, Gabriela Lago
Keller, Luiz Antonio Moura
Rosa, Carlos Alberto da Rocha
Cavaglieri, Lilia Reneé
De Azevedo, Maria Isabel
Keller, Kelly Moura
author Rodrigues, Mariana Paiva
author_facet Rodrigues, Mariana Paiva
Astoreca, Andrea Luciana
De Oliveira, Águida Aparecida
Salvato, Lauranne Alves
Biscoto, Gabriela Lago
Keller, Luiz Antonio Moura
Rosa, Carlos Alberto da Rocha
Cavaglieri, Lilia Reneé
De Azevedo, Maria Isabel
Keller, Kelly Moura
author_role author
author2 Astoreca, Andrea Luciana
De Oliveira, Águida Aparecida
Salvato, Lauranne Alves
Biscoto, Gabriela Lago
Keller, Luiz Antonio Moura
Rosa, Carlos Alberto da Rocha
Cavaglieri, Lilia Reneé
De Azevedo, Maria Isabel
Keller, Kelly Moura
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ECOPHYSIOLOGY
ESSENTIAL OILS
MYCOTOXINS
topic ECOPHYSIOLOGY
ESSENTIAL OILS
MYCOTOXINS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aspergillus carbonarius is a saprobic filamentous fungus, food spoiling fungus and a producer of ochratoxin A (OTA) mycotoxin. In this study, the in vitro antifungal activity of neem oil (0.12% p/p of azadirachtin) was evaluated against the growth of six strains of A. carbonarius and the production of OTA. Four different concentrations of neem oil were tested in addition to three incubation times. Only the concentration of 0.3% of neem oil inhibited more than 95% of the strain’s growth (97.6% ± 0.5%), while the use of 0.5% and 1.0% of neem oil showed lower antifungal activity, 40.2% ± 3.1 and 64.7% ± 1.1, respectively. There was a complete inhibition of OTA production with 0.1% and 0.3% neem oil in the four strains isolated in the laboratory from grapes. The present study shows that neem essential oil can be further evaluated as an auxiliary method for the reduction of mycelial growth and OTA production.
Fil: Rodrigues, Mariana Paiva. Universidade Federal de Minas Gerais; Brasil
Fil: Astoreca, Andrea Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: De Oliveira, Águida Aparecida. Universidad Federal Rural de Rio de Janeiro; Brasil
Fil: Salvato, Lauranne Alves. Universidade Federal de Minas Gerais; Brasil
Fil: Biscoto, Gabriela Lago. Universidade Federal de Minas Gerais; Brasil
Fil: Keller, Luiz Antonio Moura. Universidade Federal Fluminense; Brasil
Fil: Rosa, Carlos Alberto da Rocha. Universidade Federal Do Rio de Janeiro. Instituto de Biología; Brasil
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales. Departamento de Quimica. Area Fisicoquimica Organica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: De Azevedo, Maria Isabel. Universidade Federal de Minas Gerais; Brasil
Fil: Keller, Kelly Moura. Universidade Federal de Minas Gerais; Brasil
description Aspergillus carbonarius is a saprobic filamentous fungus, food spoiling fungus and a producer of ochratoxin A (OTA) mycotoxin. In this study, the in vitro antifungal activity of neem oil (0.12% p/p of azadirachtin) was evaluated against the growth of six strains of A. carbonarius and the production of OTA. Four different concentrations of neem oil were tested in addition to three incubation times. Only the concentration of 0.3% of neem oil inhibited more than 95% of the strain’s growth (97.6% ± 0.5%), while the use of 0.5% and 1.0% of neem oil showed lower antifungal activity, 40.2% ± 3.1 and 64.7% ± 1.1, respectively. There was a complete inhibition of OTA production with 0.1% and 0.3% neem oil in the four strains isolated in the laboratory from grapes. The present study shows that neem essential oil can be further evaluated as an auxiliary method for the reduction of mycelial growth and OTA production.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/122752
Rodrigues, Mariana Paiva; Astoreca, Andrea Luciana; De Oliveira, Águida Aparecida; Salvato, Lauranne Alves; Biscoto, Gabriela Lago; et al.; In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolates; Multidisciplinary Digital Publishing Institute; Toxins; 11; 10; 10-2019; 1-12
2072-6651
CONICET Digital
CONICET
url http://hdl.handle.net/11336/122752
identifier_str_mv Rodrigues, Mariana Paiva; Astoreca, Andrea Luciana; De Oliveira, Águida Aparecida; Salvato, Lauranne Alves; Biscoto, Gabriela Lago; et al.; In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolates; Multidisciplinary Digital Publishing Institute; Toxins; 11; 10; 10-2019; 1-12
2072-6651
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/toxins11100579
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6651/11/10/579
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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