Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)

Autores
Tardivo Kubis, Jonás Adrián; Rodríguez, Francisco; Rossignoli, Araceli E.; Riobó, Pilar; Sar, Eugenia Alicia; Sunesen, Inés
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1–D3 regions). Established strains belonged to Alexandrium catenella, Protoceratium reticulatum and Pseudo-nitzschia multiseries. The toxinological profile of the target strains were determined by UHPLC-FLD equipment for paralytic shellfish toxins (PSTs) and LC-MS/MS for lipophilic (LSTs) and amnesic toxins (ASTs). The toxin profile varied in the four strains of A. catenella, the predominant compounds were gonyautoxins (GTXs) GTX2,3 and GTX1,4 for strains LPCc001 and LPCc004, and N-sulfocarbamoyl toxins (Cs) C1,2 and GTX1,4 for strains LPCc002 and LPCc008. The obtained cellular toxicity values were moderate-to-high (12.38–46.40 pg saxitoxin equiv. cell−1). The toxin profile of P. reticulatum was dominated by yessotoxins (YTXs) (up to 94.40 pg cell−1) accompanied by homo-yessotoxin (Homo-YTX) traces. In P. multiseries, the toxin profiles were dominated by domoic acid (DA) (1.62 pg cell−1 and 1.09 pg cell−1) and secondarily by Isomer A (Iso-A), Epi-domoic acid (Epi-DA), Isomer-E (Iso-E) and Isomer-D (Iso-D). This study provides detailed information about representative HAB species in the area, useful for resource management, risk evaluation and related research on toxic dinoflagellates and diatoms.
Facultad de Ciencias Naturales y Museo
Materia
Ciencias Naturales
Biología
Alexandrium catenella
molecular analysis
morphology
Protoceratium reticulatum
Pseudo-nitzschia multiseries
toxinological profiles
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/160893
Acceder
id SEDICI_84e974a1743ec8565e1f562d6c6c8f66
oai_identifier_str oai:sedici.unlp.edu.ar:10915/160893
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)Tardivo Kubis, Jonás AdriánRodríguez, FranciscoRossignoli, Araceli E.Riobó, PilarSar, Eugenia AliciaSunesen, InésCiencias NaturalesBiologíaAlexandrium catenellamolecular analysismorphologyProtoceratium reticulatumPseudo-nitzschia multiseriestoxinological profilesIn the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1–D3 regions). Established strains belonged to Alexandrium catenella, Protoceratium reticulatum and Pseudo-nitzschia multiseries. The toxinological profile of the target strains were determined by UHPLC-FLD equipment for paralytic shellfish toxins (PSTs) and LC-MS/MS for lipophilic (LSTs) and amnesic toxins (ASTs). The toxin profile varied in the four strains of A. catenella, the predominant compounds were gonyautoxins (GTXs) GTX2,3 and GTX1,4 for strains LPCc001 and LPCc004, and N-sulfocarbamoyl toxins (Cs) C1,2 and GTX1,4 for strains LPCc002 and LPCc008. The obtained cellular toxicity values were moderate-to-high (12.38–46.40 pg saxitoxin equiv. cell−1). The toxin profile of P. reticulatum was dominated by yessotoxins (YTXs) (up to 94.40 pg cell−1) accompanied by homo-yessotoxin (Homo-YTX) traces. In P. multiseries, the toxin profiles were dominated by domoic acid (DA) (1.62 pg cell−1 and 1.09 pg cell−1) and secondarily by Isomer A (Iso-A), Epi-domoic acid (Epi-DA), Isomer-E (Iso-E) and Isomer-D (Iso-D). This study provides detailed information about representative HAB species in the area, useful for resource management, risk evaluation and related research on toxic dinoflagellates and diatoms.Facultad de Ciencias Naturales y Museo2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/160893enginfo:eu-repo/semantics/altIdentifier/issn/2673-9410info:eu-repo/semantics/altIdentifier/doi/10.3390/phycology3010006info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:42:10Zoai:sedici.unlp.edu.ar:10915/160893Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:42:11.218SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
spellingShingle Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
Tardivo Kubis, Jonás Adrián
Ciencias Naturales
Biología
Alexandrium catenella
molecular analysis
morphology
Protoceratium reticulatum
Pseudo-nitzschia multiseries
toxinological profiles
title_short Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title_full Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title_fullStr Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title_full_unstemmed Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title_sort Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
dc.creator.none.fl_str_mv Tardivo Kubis, Jonás Adrián
Rodríguez, Francisco
Rossignoli, Araceli E.
Riobó, Pilar
Sar, Eugenia Alicia
Sunesen, Inés
author Tardivo Kubis, Jonás Adrián
author_facet Tardivo Kubis, Jonás Adrián
Rodríguez, Francisco
Rossignoli, Araceli E.
Riobó, Pilar
Sar, Eugenia Alicia
Sunesen, Inés
author_role author
author2 Rodríguez, Francisco
Rossignoli, Araceli E.
Riobó, Pilar
Sar, Eugenia Alicia
Sunesen, Inés
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Biología
Alexandrium catenella
molecular analysis
morphology
Protoceratium reticulatum
Pseudo-nitzschia multiseries
toxinological profiles
topic Ciencias Naturales
Biología
Alexandrium catenella
molecular analysis
morphology
Protoceratium reticulatum
Pseudo-nitzschia multiseries
toxinological profiles
dc.description.none.fl_txt_mv In the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1–D3 regions). Established strains belonged to Alexandrium catenella, Protoceratium reticulatum and Pseudo-nitzschia multiseries. The toxinological profile of the target strains were determined by UHPLC-FLD equipment for paralytic shellfish toxins (PSTs) and LC-MS/MS for lipophilic (LSTs) and amnesic toxins (ASTs). The toxin profile varied in the four strains of A. catenella, the predominant compounds were gonyautoxins (GTXs) GTX2,3 and GTX1,4 for strains LPCc001 and LPCc004, and N-sulfocarbamoyl toxins (Cs) C1,2 and GTX1,4 for strains LPCc002 and LPCc008. The obtained cellular toxicity values were moderate-to-high (12.38–46.40 pg saxitoxin equiv. cell−1). The toxin profile of P. reticulatum was dominated by yessotoxins (YTXs) (up to 94.40 pg cell−1) accompanied by homo-yessotoxin (Homo-YTX) traces. In P. multiseries, the toxin profiles were dominated by domoic acid (DA) (1.62 pg cell−1 and 1.09 pg cell−1) and secondarily by Isomer A (Iso-A), Epi-domoic acid (Epi-DA), Isomer-E (Iso-E) and Isomer-D (Iso-D). This study provides detailed information about representative HAB species in the area, useful for resource management, risk evaluation and related research on toxic dinoflagellates and diatoms.
Facultad de Ciencias Naturales y Museo
description In the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1–D3 regions). Established strains belonged to Alexandrium catenella, Protoceratium reticulatum and Pseudo-nitzschia multiseries. The toxinological profile of the target strains were determined by UHPLC-FLD equipment for paralytic shellfish toxins (PSTs) and LC-MS/MS for lipophilic (LSTs) and amnesic toxins (ASTs). The toxin profile varied in the four strains of A. catenella, the predominant compounds were gonyautoxins (GTXs) GTX2,3 and GTX1,4 for strains LPCc001 and LPCc004, and N-sulfocarbamoyl toxins (Cs) C1,2 and GTX1,4 for strains LPCc002 and LPCc008. The obtained cellular toxicity values were moderate-to-high (12.38–46.40 pg saxitoxin equiv. cell−1). The toxin profile of P. reticulatum was dominated by yessotoxins (YTXs) (up to 94.40 pg cell−1) accompanied by homo-yessotoxin (Homo-YTX) traces. In P. multiseries, the toxin profiles were dominated by domoic acid (DA) (1.62 pg cell−1 and 1.09 pg cell−1) and secondarily by Isomer A (Iso-A), Epi-domoic acid (Epi-DA), Isomer-E (Iso-E) and Isomer-D (Iso-D). This study provides detailed information about representative HAB species in the area, useful for resource management, risk evaluation and related research on toxic dinoflagellates and diatoms.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/160893
url http://sedici.unlp.edu.ar/handle/10915/160893
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2673-9410
info:eu-repo/semantics/altIdentifier/doi/10.3390/phycology3010006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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