Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)

Autores: Tardivo Kubis, Jonás Adrián; Rodríguez, Francisco; Rossignoli, Araceli E.; Riobó, Pilar; Sar, Eugenia Alicia; Sunesen, Inés
Año de publicación: 2023
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: In the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1?D3 regions). Established strains belonged to Alexandrium catenella, Protoceratium reticulatum and Pseudo-nitzschia multiseries. The toxinological profile of the target strains were determined by UHPLC-FLD equipment for paralytic shellfish toxins (PSTs) and LC-MS/MS for lipophilic (LSTs) and amnesic toxins (ASTs). The toxin profile varied in the four strains of A. catenella, the predominant compounds were gonyautoxins (GTXs) GTX2,3 and GTX1,4 for strains LPCc001 and LPCc004, and N-sulfocarbamoyl toxins (Cs) C1,2 and GTX1,4 for strains LPCc002 and LPCc008. The obtained cellular toxicity values were moderate-to-high (12.38?46.40 pg saxitoxin equiv. cell−1). The toxin profile of P. reticulatum was dominated by yessotoxins (YTXs) (up to 94.40 pg cell−1) accompanied by homo-yessotoxin (Homo-YTX) traces. In P. multiseries, the toxin profiles were dominated by domoic acid (DA) (1.62 pg cell−1 and 1.09 pg cell−1) and secondarily by Isomer A (Iso-A), Epi-domoic acid (Epi-DA), Isomer-E (Iso-E) and Isomer-D (Iso-D). This study provides detailed information about representative HAB species in the area, useful for resource management, risk evaluation and related research on toxic dinoflagellates and diatoms.
Fil: Tardivo Kubis, Jonás Adrián. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina
Fil: Rodríguez, Francisco. Instituto de Oceanografía. Centro Oceanográfico de Vigo; España
Fil: Rossignoli, Araceli E.. Centro de Investigaciones Mariñas; España
Fil: Riobó, Pilar. Consejo Superior de Investigaciones Cientificas. Instituto de Investigaciones Marinas.; España
Fil: Sar, Eugenia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina
Fil: Sunesen, Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina
Materia: ALEXANDRIUM CATENELLA
MOLECULAR ANALYSIS
MORPHOLOGY
PROTOCERATIUM RETICULATUM
PSEUDO-NITZSCHIA MULTISERIES
TOXINOLOGICAL PROFILES
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/221964

Acceder

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network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)Tardivo Kubis, Jonás AdriánRodríguez, FranciscoRossignoli, Araceli E.Riobó, PilarSar, Eugenia AliciaSunesen, InésALEXANDRIUM CATENELLAMOLECULAR ANALYSISMORPHOLOGYPROTOCERATIUM RETICULATUMPSEUDO-NITZSCHIA MULTISERIESTOXINOLOGICAL PROFILEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1?D3 regions). Established strains belonged to Alexandrium catenella, Protoceratium reticulatum and Pseudo-nitzschia multiseries. The toxinological profile of the target strains were determined by UHPLC-FLD equipment for paralytic shellfish toxins (PSTs) and LC-MS/MS for lipophilic (LSTs) and amnesic toxins (ASTs). The toxin profile varied in the four strains of A. catenella, the predominant compounds were gonyautoxins (GTXs) GTX2,3 and GTX1,4 for strains LPCc001 and LPCc004, and N-sulfocarbamoyl toxins (Cs) C1,2 and GTX1,4 for strains LPCc002 and LPCc008. The obtained cellular toxicity values were moderate-to-high (12.38?46.40 pg saxitoxin equiv. cell−1). The toxin profile of P. reticulatum was dominated by yessotoxins (YTXs) (up to 94.40 pg cell−1) accompanied by homo-yessotoxin (Homo-YTX) traces. In P. multiseries, the toxin profiles were dominated by domoic acid (DA) (1.62 pg cell−1 and 1.09 pg cell−1) and secondarily by Isomer A (Iso-A), Epi-domoic acid (Epi-DA), Isomer-E (Iso-E) and Isomer-D (Iso-D). This study provides detailed information about representative HAB species in the area, useful for resource management, risk evaluation and related research on toxic dinoflagellates and diatoms.Fil: Tardivo Kubis, Jonás Adrián. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; ArgentinaFil: Rodríguez, Francisco. Instituto de Oceanografía. Centro Oceanográfico de Vigo; EspañaFil: Rossignoli, Araceli E.. Centro de Investigaciones Mariñas; EspañaFil: Riobó, Pilar. Consejo Superior de Investigaciones Cientificas. Instituto de Investigaciones Marinas.; EspañaFil: Sar, Eugenia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; ArgentinaFil: Sunesen, Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; ArgentinaMultidisciplinary Digital Publishing Institute2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/221964Tardivo Kubis, Jonás Adrián; Rodríguez, Francisco; Rossignoli, Araceli E.; Riobó, Pilar; Sar, Eugenia Alicia; et al.; Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina); Multidisciplinary Digital Publishing Institute; Phycology; 3; 1; 2-2023; 79-1052673-9410CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-9410/3/1/6info:eu-repo/semantics/altIdentifier/doi/10.3390/phycology3010006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:52Zoai:ri.conicet.gov.ar:11336/221964instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:52.825CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title	Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
spellingShingle	Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina) Tardivo Kubis, Jonás Adrián ALEXANDRIUM CATENELLA MOLECULAR ANALYSIS MORPHOLOGY PROTOCERATIUM RETICULATUM PSEUDO-NITZSCHIA MULTISERIES TOXINOLOGICAL PROFILES
title_short	Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title_full	Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title_fullStr	Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title_full_unstemmed	Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title_sort	Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
dc.creator.none.fl_str_mv	Tardivo Kubis, Jonás Adrián Rodríguez, Francisco Rossignoli, Araceli E. Riobó, Pilar Sar, Eugenia Alicia Sunesen, Inés
author	Tardivo Kubis, Jonás Adrián
author_facet	Tardivo Kubis, Jonás Adrián Rodríguez, Francisco Rossignoli, Araceli E. Riobó, Pilar Sar, Eugenia Alicia Sunesen, Inés
author_role	author
author2	Rodríguez, Francisco Rossignoli, Araceli E. Riobó, Pilar Sar, Eugenia Alicia Sunesen, Inés
author2_role	author author author author author
dc.subject.none.fl_str_mv	ALEXANDRIUM CATENELLA MOLECULAR ANALYSIS MORPHOLOGY PROTOCERATIUM RETICULATUM PSEUDO-NITZSCHIA MULTISERIES TOXINOLOGICAL PROFILES
topic	ALEXANDRIUM CATENELLA MOLECULAR ANALYSIS MORPHOLOGY PROTOCERATIUM RETICULATUM PSEUDO-NITZSCHIA MULTISERIES TOXINOLOGICAL PROFILES
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	In the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1?D3 regions). Established strains belonged to Alexandrium catenella, Protoceratium reticulatum and Pseudo-nitzschia multiseries. The toxinological profile of the target strains were determined by UHPLC-FLD equipment for paralytic shellfish toxins (PSTs) and LC-MS/MS for lipophilic (LSTs) and amnesic toxins (ASTs). The toxin profile varied in the four strains of A. catenella, the predominant compounds were gonyautoxins (GTXs) GTX2,3 and GTX1,4 for strains LPCc001 and LPCc004, and N-sulfocarbamoyl toxins (Cs) C1,2 and GTX1,4 for strains LPCc002 and LPCc008. The obtained cellular toxicity values were moderate-to-high (12.38?46.40 pg saxitoxin equiv. cell−1). The toxin profile of P. reticulatum was dominated by yessotoxins (YTXs) (up to 94.40 pg cell−1) accompanied by homo-yessotoxin (Homo-YTX) traces. In P. multiseries, the toxin profiles were dominated by domoic acid (DA) (1.62 pg cell−1 and 1.09 pg cell−1) and secondarily by Isomer A (Iso-A), Epi-domoic acid (Epi-DA), Isomer-E (Iso-E) and Isomer-D (Iso-D). This study provides detailed information about representative HAB species in the area, useful for resource management, risk evaluation and related research on toxic dinoflagellates and diatoms. Fil: Tardivo Kubis, Jonás Adrián. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina Fil: Rodríguez, Francisco. Instituto de Oceanografía. Centro Oceanográfico de Vigo; España Fil: Rossignoli, Araceli E.. Centro de Investigaciones Mariñas; España Fil: Riobó, Pilar. Consejo Superior de Investigaciones Cientificas. Instituto de Investigaciones Marinas.; España Fil: Sar, Eugenia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina Fil: Sunesen, Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina
description	In the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1?D3 regions). Established strains belonged to Alexandrium catenella, Protoceratium reticulatum and Pseudo-nitzschia multiseries. The toxinological profile of the target strains were determined by UHPLC-FLD equipment for paralytic shellfish toxins (PSTs) and LC-MS/MS for lipophilic (LSTs) and amnesic toxins (ASTs). The toxin profile varied in the four strains of A. catenella, the predominant compounds were gonyautoxins (GTXs) GTX2,3 and GTX1,4 for strains LPCc001 and LPCc004, and N-sulfocarbamoyl toxins (Cs) C1,2 and GTX1,4 for strains LPCc002 and LPCc008. The obtained cellular toxicity values were moderate-to-high (12.38?46.40 pg saxitoxin equiv. cell−1). The toxin profile of P. reticulatum was dominated by yessotoxins (YTXs) (up to 94.40 pg cell−1) accompanied by homo-yessotoxin (Homo-YTX) traces. In P. multiseries, the toxin profiles were dominated by domoic acid (DA) (1.62 pg cell−1 and 1.09 pg cell−1) and secondarily by Isomer A (Iso-A), Epi-domoic acid (Epi-DA), Isomer-E (Iso-E) and Isomer-D (Iso-D). This study provides detailed information about representative HAB species in the area, useful for resource management, risk evaluation and related research on toxic dinoflagellates and diatoms.
publishDate	2023
dc.date.none.fl_str_mv	2023-02
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/221964 Tardivo Kubis, Jonás Adrián; Rodríguez, Francisco; Rossignoli, Araceli E.; Riobó, Pilar; Sar, Eugenia Alicia; et al.; Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina); Multidisciplinary Digital Publishing Institute; Phycology; 3; 1; 2-2023; 79-105 2673-9410 CONICET Digital CONICET
url	http://hdl.handle.net/11336/221964
identifier_str_mv	Tardivo Kubis, Jonás Adrián; Rodríguez, Francisco; Rossignoli, Araceli E.; Riobó, Pilar; Sar, Eugenia Alicia; et al.; Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina); Multidisciplinary Digital Publishing Institute; Phycology; 3; 1; 2-2023; 79-105 2673-9410 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-9410/3/1/6 info:eu-repo/semantics/altIdentifier/doi/10.3390/phycology3010006
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv	Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
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instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)

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