Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of Argentina

Autores
Akselman Cardella, Rut; Krock, Bernd; Alpermann, Tilman J.; Tillmann, Urban; Borel, Claudia Marcela; Almandoz, Gaston Osvaldo; Ferrario, Martha Elba
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Protoceratium reticulatum is a dinoflagellate with a life cycle that includes a motile planktonic stage and a resting cyst stage of benthic habitat, both with a wide geographical distribution, including southern South America. P. reticulatum produces yessotoxins (YTXs) which are accumulated in shellfish, show potent cytotoxicity and pose a danger to human health via consumption of contaminated seafood. YTXs have been reported from coastal shellfish of many localities, but until now it was unknown if they were present at the austral Southwestern Atlantic and also if local populations of P. reticulatum have the ability to produce these toxins. In this study we report the presence of YTXs in plankton samples and its production in culture by two P. reticulatum strains isolated from the San Jorge Gulf (SJG). In addition, we describe the geographical distribution and seasonal abundance of this species based on data collected over the past two decades. The YTX cell quotas calculated from net hauls (~10 pg cell?1) are in the same range as the toxin cell quotas observed in these two isolates. The phylogenetic analysis of sequences of the hypervariable region of the large subunit (LSU) 28S rDNA showed that the two clonal strains from the SJG were part of a monophyletic clade that subdivides P. reticulatum into two well supported, divergent sub-clades. The sequences of the two strains of P. reticulatum from the SJG fell in the same clade as the majority of sequences of P. reticulatum, which belong to a geographically wide distributed evolutionary clade. P. reticulatum was occasionally observed from about 35°S in Uruguayan shelf waters up to 53°S in the Patagonian shelf and north of Tierra del Fuego, and it was present from coastal areas up to the shelf break zone. We recorded P. reticulatum in plankton samples from spring to autumn, throughout the warmer period of the year, and invariably in low abundance (maximum: 560 cells L?1). Viable cysts of the species in surface sediments also showed a wide geographical distribution, which, together with high total abundances and high relative numerical contribution to planktonic dinoflagellate assemblages near frontal areas, emphasize the necessity to pay attention to the dynamics of this species in areas of potential risk of harmful algal bloom development.
Fil: Akselman Cardella, Rut. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Krock, Bernd. Alfred Wegener Institute for Polar and Marine Research; Alemania
Fil: Alpermann, Tilman J.. Senckenberg Research Institute and Natural History Museum; Alemania
Fil: Tillmann, Urban. Alfred Wegener Institute for Polar and Marine Research; Alemania
Fil: Borel, Claudia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Geológico del Sur. Universidad Nacional del Sur. Departamento de Geología. Instituto Geológico del Sur; Argentina
Fil: Almandoz, Gaston Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
Fil: Ferrario, Martha Elba. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
Materia
Protoceratium Reticulatum
Dinoflagellates
Southwestern Atlantic
Phylogeny
Yessotoxins
Cysts
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47636

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network_name_str CONICET Digital (CONICET)
spelling Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of ArgentinaAkselman Cardella, RutKrock, BerndAlpermann, Tilman J.Tillmann, UrbanBorel, Claudia MarcelaAlmandoz, Gaston OsvaldoFerrario, Martha ElbaProtoceratium ReticulatumDinoflagellatesSouthwestern AtlanticPhylogenyYessotoxinsCystshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Protoceratium reticulatum is a dinoflagellate with a life cycle that includes a motile planktonic stage and a resting cyst stage of benthic habitat, both with a wide geographical distribution, including southern South America. P. reticulatum produces yessotoxins (YTXs) which are accumulated in shellfish, show potent cytotoxicity and pose a danger to human health via consumption of contaminated seafood. YTXs have been reported from coastal shellfish of many localities, but until now it was unknown if they were present at the austral Southwestern Atlantic and also if local populations of P. reticulatum have the ability to produce these toxins. In this study we report the presence of YTXs in plankton samples and its production in culture by two P. reticulatum strains isolated from the San Jorge Gulf (SJG). In addition, we describe the geographical distribution and seasonal abundance of this species based on data collected over the past two decades. The YTX cell quotas calculated from net hauls (~10 pg cell?1) are in the same range as the toxin cell quotas observed in these two isolates. The phylogenetic analysis of sequences of the hypervariable region of the large subunit (LSU) 28S rDNA showed that the two clonal strains from the SJG were part of a monophyletic clade that subdivides P. reticulatum into two well supported, divergent sub-clades. The sequences of the two strains of P. reticulatum from the SJG fell in the same clade as the majority of sequences of P. reticulatum, which belong to a geographically wide distributed evolutionary clade. P. reticulatum was occasionally observed from about 35°S in Uruguayan shelf waters up to 53°S in the Patagonian shelf and north of Tierra del Fuego, and it was present from coastal areas up to the shelf break zone. We recorded P. reticulatum in plankton samples from spring to autumn, throughout the warmer period of the year, and invariably in low abundance (maximum: 560 cells L?1). Viable cysts of the species in surface sediments also showed a wide geographical distribution, which, together with high total abundances and high relative numerical contribution to planktonic dinoflagellate assemblages near frontal areas, emphasize the necessity to pay attention to the dynamics of this species in areas of potential risk of harmful algal bloom development.Fil: Akselman Cardella, Rut. Instituto Nacional de Investigaciones y Desarrollo Pesquero; ArgentinaFil: Krock, Bernd. Alfred Wegener Institute for Polar and Marine Research; AlemaniaFil: Alpermann, Tilman J.. Senckenberg Research Institute and Natural History Museum; AlemaniaFil: Tillmann, Urban. Alfred Wegener Institute for Polar and Marine Research; AlemaniaFil: Borel, Claudia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Geológico del Sur. Universidad Nacional del Sur. Departamento de Geología. Instituto Geológico del Sur; ArgentinaFil: Almandoz, Gaston Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Ferrario, Martha Elba. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaElsevier Science2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47636Akselman Cardella, Rut; Krock, Bernd; Alpermann, Tilman J.; Tillmann, Urban; Borel, Claudia Marcela; et al.; Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of Argentina; Elsevier Science; Harmful Algae; 45; 5-2015; 40-521568-9883CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.hal.2015.03.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1568988315000554info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:31Zoai:ri.conicet.gov.ar:11336/47636instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:31.615CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of Argentina
title Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of Argentina
spellingShingle Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of Argentina
Akselman Cardella, Rut
Protoceratium Reticulatum
Dinoflagellates
Southwestern Atlantic
Phylogeny
Yessotoxins
Cysts
title_short Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of Argentina
title_full Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of Argentina
title_fullStr Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of Argentina
title_full_unstemmed Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of Argentina
title_sort Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of Argentina
dc.creator.none.fl_str_mv Akselman Cardella, Rut
Krock, Bernd
Alpermann, Tilman J.
Tillmann, Urban
Borel, Claudia Marcela
Almandoz, Gaston Osvaldo
Ferrario, Martha Elba
author Akselman Cardella, Rut
author_facet Akselman Cardella, Rut
Krock, Bernd
Alpermann, Tilman J.
Tillmann, Urban
Borel, Claudia Marcela
Almandoz, Gaston Osvaldo
Ferrario, Martha Elba
author_role author
author2 Krock, Bernd
Alpermann, Tilman J.
Tillmann, Urban
Borel, Claudia Marcela
Almandoz, Gaston Osvaldo
Ferrario, Martha Elba
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Protoceratium Reticulatum
Dinoflagellates
Southwestern Atlantic
Phylogeny
Yessotoxins
Cysts
topic Protoceratium Reticulatum
Dinoflagellates
Southwestern Atlantic
Phylogeny
Yessotoxins
Cysts
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Protoceratium reticulatum is a dinoflagellate with a life cycle that includes a motile planktonic stage and a resting cyst stage of benthic habitat, both with a wide geographical distribution, including southern South America. P. reticulatum produces yessotoxins (YTXs) which are accumulated in shellfish, show potent cytotoxicity and pose a danger to human health via consumption of contaminated seafood. YTXs have been reported from coastal shellfish of many localities, but until now it was unknown if they were present at the austral Southwestern Atlantic and also if local populations of P. reticulatum have the ability to produce these toxins. In this study we report the presence of YTXs in plankton samples and its production in culture by two P. reticulatum strains isolated from the San Jorge Gulf (SJG). In addition, we describe the geographical distribution and seasonal abundance of this species based on data collected over the past two decades. The YTX cell quotas calculated from net hauls (~10 pg cell?1) are in the same range as the toxin cell quotas observed in these two isolates. The phylogenetic analysis of sequences of the hypervariable region of the large subunit (LSU) 28S rDNA showed that the two clonal strains from the SJG were part of a monophyletic clade that subdivides P. reticulatum into two well supported, divergent sub-clades. The sequences of the two strains of P. reticulatum from the SJG fell in the same clade as the majority of sequences of P. reticulatum, which belong to a geographically wide distributed evolutionary clade. P. reticulatum was occasionally observed from about 35°S in Uruguayan shelf waters up to 53°S in the Patagonian shelf and north of Tierra del Fuego, and it was present from coastal areas up to the shelf break zone. We recorded P. reticulatum in plankton samples from spring to autumn, throughout the warmer period of the year, and invariably in low abundance (maximum: 560 cells L?1). Viable cysts of the species in surface sediments also showed a wide geographical distribution, which, together with high total abundances and high relative numerical contribution to planktonic dinoflagellate assemblages near frontal areas, emphasize the necessity to pay attention to the dynamics of this species in areas of potential risk of harmful algal bloom development.
Fil: Akselman Cardella, Rut. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Krock, Bernd. Alfred Wegener Institute for Polar and Marine Research; Alemania
Fil: Alpermann, Tilman J.. Senckenberg Research Institute and Natural History Museum; Alemania
Fil: Tillmann, Urban. Alfred Wegener Institute for Polar and Marine Research; Alemania
Fil: Borel, Claudia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Geológico del Sur. Universidad Nacional del Sur. Departamento de Geología. Instituto Geológico del Sur; Argentina
Fil: Almandoz, Gaston Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
Fil: Ferrario, Martha Elba. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
description Protoceratium reticulatum is a dinoflagellate with a life cycle that includes a motile planktonic stage and a resting cyst stage of benthic habitat, both with a wide geographical distribution, including southern South America. P. reticulatum produces yessotoxins (YTXs) which are accumulated in shellfish, show potent cytotoxicity and pose a danger to human health via consumption of contaminated seafood. YTXs have been reported from coastal shellfish of many localities, but until now it was unknown if they were present at the austral Southwestern Atlantic and also if local populations of P. reticulatum have the ability to produce these toxins. In this study we report the presence of YTXs in plankton samples and its production in culture by two P. reticulatum strains isolated from the San Jorge Gulf (SJG). In addition, we describe the geographical distribution and seasonal abundance of this species based on data collected over the past two decades. The YTX cell quotas calculated from net hauls (~10 pg cell?1) are in the same range as the toxin cell quotas observed in these two isolates. The phylogenetic analysis of sequences of the hypervariable region of the large subunit (LSU) 28S rDNA showed that the two clonal strains from the SJG were part of a monophyletic clade that subdivides P. reticulatum into two well supported, divergent sub-clades. The sequences of the two strains of P. reticulatum from the SJG fell in the same clade as the majority of sequences of P. reticulatum, which belong to a geographically wide distributed evolutionary clade. P. reticulatum was occasionally observed from about 35°S in Uruguayan shelf waters up to 53°S in the Patagonian shelf and north of Tierra del Fuego, and it was present from coastal areas up to the shelf break zone. We recorded P. reticulatum in plankton samples from spring to autumn, throughout the warmer period of the year, and invariably in low abundance (maximum: 560 cells L?1). Viable cysts of the species in surface sediments also showed a wide geographical distribution, which, together with high total abundances and high relative numerical contribution to planktonic dinoflagellate assemblages near frontal areas, emphasize the necessity to pay attention to the dynamics of this species in areas of potential risk of harmful algal bloom development.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47636
Akselman Cardella, Rut; Krock, Bernd; Alpermann, Tilman J.; Tillmann, Urban; Borel, Claudia Marcela; et al.; Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of Argentina; Elsevier Science; Harmful Algae; 45; 5-2015; 40-52
1568-9883
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47636
identifier_str_mv Akselman Cardella, Rut; Krock, Bernd; Alpermann, Tilman J.; Tillmann, Urban; Borel, Claudia Marcela; et al.; Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of Argentina; Elsevier Science; Harmful Algae; 45; 5-2015; 40-52
1568-9883
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.hal.2015.03.001
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1568988315000554
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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application/pdf
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dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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