Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells

Autores
Minnaard, Jessica; Lievin Le Moal, Vanessa; Coconnier, Marie Helene; Servin, Alain L.; Pérez, Pablo Fernando
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present study, the role of direct procaryote-eucaryote interactions in the virulence of Bacillus cereus was investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with B. cereus in the exponential phase of growth, in order to minimize the concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect develops. Interestingly, addition of 3-h-old cultures of some strains resulted in complete detachment of the cultured cells after a 3-h infection whereas no such effect was found after a 3-h infection with 16-h-old cultures. Infection of enterocyte-like cells with B. cereus leads to disruption of the F-actin network and necrosis. Even though the effect of secreted factors cannot be ruled out, direct eucaryote-procaryote interaction seems to be necessary. In addition, we observed that some B. cereus strains were able to be internalized in Caco-2 cells. Our findings add a new insight into the mechanisms of virulence of B. cereus in the context of intestinal infection.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Materia
Ciencias Exactas
Bacillus cereus
intestinal infection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/84452
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/84452
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cellsMinnaard, JessicaLievin Le Moal, VanessaCoconnier, Marie HeleneServin, Alain L.Pérez, Pablo FernandoCiencias ExactasBacillus cereusintestinal infectionIn the present study, the role of direct procaryote-eucaryote interactions in the virulence of <i>Bacillus cereus</i> was investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with <i>B. cereus</i> in the exponential phase of growth, in order to minimize the concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect develops. Interestingly, addition of 3-h-old cultures of some strains resulted in complete detachment of the cultured cells after a 3-h infection whereas no such effect was found after a 3-h infection with 16-h-old cultures. Infection of enterocyte-like cells with <i>B. cereus</i> leads to disruption of the F-actin network and necrosis. Even though the effect of secreted factors cannot be ruled out, direct eucaryote-procaryote interaction seems to be necessary. In addition, we observed that some <i>B. cereus</i> strains were able to be internalized in Caco-2 cells. Our findings add a new insight into the mechanisms of virulence of <i>B. cereus</i> in the context of intestinal infection.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Criotecnología de Alimentos2004info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf3106-3112http://sedici.unlp.edu.ar/handle/10915/84452enginfo:eu-repo/semantics/altIdentifier/issn/0019-9567info:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.72.6.3106-3112.2004info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T16:57:06Zoai:sedici.unlp.edu.ar:10915/84452Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 16:57:06.883SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
spellingShingle Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
Minnaard, Jessica
Ciencias Exactas
Bacillus cereus
intestinal infection
title_short Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title_full Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title_fullStr Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title_full_unstemmed Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title_sort Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
dc.creator.none.fl_str_mv Minnaard, Jessica
Lievin Le Moal, Vanessa
Coconnier, Marie Helene
Servin, Alain L.
Pérez, Pablo Fernando
author Minnaard, Jessica
author_facet Minnaard, Jessica
Lievin Le Moal, Vanessa
Coconnier, Marie Helene
Servin, Alain L.
Pérez, Pablo Fernando
author_role author
author2 Lievin Le Moal, Vanessa
Coconnier, Marie Helene
Servin, Alain L.
Pérez, Pablo Fernando
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Bacillus cereus
intestinal infection
topic Ciencias Exactas
Bacillus cereus
intestinal infection
dc.description.none.fl_txt_mv In the present study, the role of direct procaryote-eucaryote interactions in the virulence of <i>Bacillus cereus</i> was investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with <i>B. cereus</i> in the exponential phase of growth, in order to minimize the concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect develops. Interestingly, addition of 3-h-old cultures of some strains resulted in complete detachment of the cultured cells after a 3-h infection whereas no such effect was found after a 3-h infection with 16-h-old cultures. Infection of enterocyte-like cells with <i>B. cereus</i> leads to disruption of the F-actin network and necrosis. Even though the effect of secreted factors cannot be ruled out, direct eucaryote-procaryote interaction seems to be necessary. In addition, we observed that some <i>B. cereus</i> strains were able to be internalized in Caco-2 cells. Our findings add a new insight into the mechanisms of virulence of <i>B. cereus</i> in the context of intestinal infection.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
description In the present study, the role of direct procaryote-eucaryote interactions in the virulence of <i>Bacillus cereus</i> was investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with <i>B. cereus</i> in the exponential phase of growth, in order to minimize the concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect develops. Interestingly, addition of 3-h-old cultures of some strains resulted in complete detachment of the cultured cells after a 3-h infection whereas no such effect was found after a 3-h infection with 16-h-old cultures. Infection of enterocyte-like cells with <i>B. cereus</i> leads to disruption of the F-actin network and necrosis. Even though the effect of secreted factors cannot be ruled out, direct eucaryote-procaryote interaction seems to be necessary. In addition, we observed that some <i>B. cereus</i> strains were able to be internalized in Caco-2 cells. Our findings add a new insight into the mechanisms of virulence of <i>B. cereus</i> in the context of intestinal infection.
publishDate 2004
dc.date.none.fl_str_mv 2004
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/84452
url http://sedici.unlp.edu.ar/handle/10915/84452
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0019-9567
info:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.72.6.3106-3112.2004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
3106-3112
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 12.982451

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