Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells

Autores
Minnaard, Jessica; Lievin Le Moal, Vanessa; Coconnier, Marie Helene; Servin, Alain L.; Perez, Pablo Fernando
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present study the role of direct procaryote-eucaryote interactions in the virulence of Bacillus cereus has been investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with B. cereus in exponential phase of growth, in order to minimize the concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect develops. Interestingly, three hours-old cultures of some strains completely detached the cultured cells after 3 h post-infection, whereas no such effect was found with 16 h-old cultures. Infection of enterocyte-like cells with B. cereus leads to disruption of the F-actin network and necrosis. Even though, the effect of secreted factors cannot be ruled out, the direct eucaryote-procaryote interaction seems to be necessary. In addition, we observed that some B. cereus strains were able to internalize into Caco-2 cells. Our findings add a new insight on the mechanisms of virulence of B. cereus in the context of intestinal infection.
Fil: Minnaard, Jessica. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Lievin Le Moal, Vanessa. Inserm; Francia
Fil: Coconnier, Marie Helene. Inserm; Francia
Fil: Servin, Alain L.. Inserm; Francia
Fil: Perez, Pablo Fernando. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Materia
Bacillus cereus
Human Intestinal
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/158334
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/158334
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 CellsMinnaard, JessicaLievin Le Moal, VanessaCoconnier, Marie HeleneServin, Alain L.Perez, Pablo FernandoBacillus cereusHuman Intestinalhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2In the present study the role of direct procaryote-eucaryote interactions in the virulence of Bacillus cereus has been investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with B. cereus in exponential phase of growth, in order to minimize the concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect develops. Interestingly, three hours-old cultures of some strains completely detached the cultured cells after 3 h post-infection, whereas no such effect was found with 16 h-old cultures. Infection of enterocyte-like cells with B. cereus leads to disruption of the F-actin network and necrosis. Even though, the effect of secreted factors cannot be ruled out, the direct eucaryote-procaryote interaction seems to be necessary. In addition, we observed that some B. cereus strains were able to internalize into Caco-2 cells. Our findings add a new insight on the mechanisms of virulence of B. cereus in the context of intestinal infection.Fil: Minnaard, Jessica. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Lievin Le Moal, Vanessa. Inserm; FranciaFil: Coconnier, Marie Helene. Inserm; FranciaFil: Servin, Alain L.. Inserm; FranciaFil: Perez, Pablo Fernando. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaAmerican Society for Microbiology2004-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158334Minnaard, Jessica; Lievin Le Moal, Vanessa; Coconnier, Marie Helene; Servin, Alain L.; Perez, Pablo Fernando; Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells; American Society for Microbiology; Infection and Immunity; 72; 6; 6-2004; 3106-31120019-95671098-5522CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/IAI.72.6.3106-3112.2004info:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.72.6.3106-3112.2004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:55Zoai:ri.conicet.gov.ar:11336/158334instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:56.003CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells
title Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells
spellingShingle Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells
Minnaard, Jessica
Bacillus cereus
Human Intestinal
title_short Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells
title_full Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells
title_fullStr Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells
title_full_unstemmed Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells
title_sort Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells
dc.creator.none.fl_str_mv Minnaard, Jessica
Lievin Le Moal, Vanessa
Coconnier, Marie Helene
Servin, Alain L.
Perez, Pablo Fernando
author Minnaard, Jessica
author_facet Minnaard, Jessica
Lievin Le Moal, Vanessa
Coconnier, Marie Helene
Servin, Alain L.
Perez, Pablo Fernando
author_role author
author2 Lievin Le Moal, Vanessa
Coconnier, Marie Helene
Servin, Alain L.
Perez, Pablo Fernando
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bacillus cereus
Human Intestinal
topic Bacillus cereus
Human Intestinal
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In the present study the role of direct procaryote-eucaryote interactions in the virulence of Bacillus cereus has been investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with B. cereus in exponential phase of growth, in order to minimize the concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect develops. Interestingly, three hours-old cultures of some strains completely detached the cultured cells after 3 h post-infection, whereas no such effect was found with 16 h-old cultures. Infection of enterocyte-like cells with B. cereus leads to disruption of the F-actin network and necrosis. Even though, the effect of secreted factors cannot be ruled out, the direct eucaryote-procaryote interaction seems to be necessary. In addition, we observed that some B. cereus strains were able to internalize into Caco-2 cells. Our findings add a new insight on the mechanisms of virulence of B. cereus in the context of intestinal infection.
Fil: Minnaard, Jessica. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Lievin Le Moal, Vanessa. Inserm; Francia
Fil: Coconnier, Marie Helene. Inserm; Francia
Fil: Servin, Alain L.. Inserm; Francia
Fil: Perez, Pablo Fernando. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
description In the present study the role of direct procaryote-eucaryote interactions in the virulence of Bacillus cereus has been investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with B. cereus in exponential phase of growth, in order to minimize the concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect develops. Interestingly, three hours-old cultures of some strains completely detached the cultured cells after 3 h post-infection, whereas no such effect was found with 16 h-old cultures. Infection of enterocyte-like cells with B. cereus leads to disruption of the F-actin network and necrosis. Even though, the effect of secreted factors cannot be ruled out, the direct eucaryote-procaryote interaction seems to be necessary. In addition, we observed that some B. cereus strains were able to internalize into Caco-2 cells. Our findings add a new insight on the mechanisms of virulence of B. cereus in the context of intestinal infection.
publishDate 2004
dc.date.none.fl_str_mv 2004-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/158334
Minnaard, Jessica; Lievin Le Moal, Vanessa; Coconnier, Marie Helene; Servin, Alain L.; Perez, Pablo Fernando; Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells; American Society for Microbiology; Infection and Immunity; 72; 6; 6-2004; 3106-3112
0019-9567
1098-5522
CONICET Digital
CONICET
url http://hdl.handle.net/11336/158334
identifier_str_mv Minnaard, Jessica; Lievin Le Moal, Vanessa; Coconnier, Marie Helene; Servin, Alain L.; Perez, Pablo Fernando; Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells; American Society for Microbiology; Infection and Immunity; 72; 6; 6-2004; 3106-3112
0019-9567
1098-5522
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/IAI.72.6.3106-3112.2004
info:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.72.6.3106-3112.2004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269826808020992
score 13.13397

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