Development of a Competitive Enzyme Immunoassay Technique for the Detection of Soy Traces in Meat Products

Autores
Cellerino, Karina; Rodríguez, Viviana Gladys; Docena, Guillermo Horacio; López, Laura Beatriz
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to develop a competitive enzyme immunoassay technique, to detect the presence of traces of soy in meat products. Specific rabbit polyclonal antiserum against soy protein was used as primary antibody. The optimal antigen concentration to be immobilized on the plate and the concentration of primary antibody to be used in competition was determined. The calibration curve was fitted using increasing concentrations of an extract of soy product. The soy product was extracted with Tris-HCl buffer 0.0625M with 3% sodium dodecylsulfate and 2% mercaptoethanol. The working range used in the enzyme immunoassay to detect soy was 9-280ppm SP with adequate linearity (R2: 0.9880). All validation parameters studied were appropriate. Commercial samples of meat products were analyzed with this enzyme immunoassays and a commercial ELISA kit. Significant differences were observed in the quantitative results obtained with both methods; nevertheless the developed enzyme immunoassay could be used as screening method.
Facultad de Ciencias Exactas
Instituto de Estudios Inmunológicos y Fisiopatológicos
Materia
Ciencias Exactas
Química
ELISA
Allergens
Soy detection
Meat products
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/99255
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/99255
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Development of a Competitive Enzyme Immunoassay Technique for the Detection of Soy Traces in Meat ProductsCellerino, KarinaRodríguez, Viviana GladysDocena, Guillermo HoracioLópez, Laura BeatrizCiencias ExactasQuímicaELISAAllergensSoy detectionMeat productsThe aim of this work was to develop a competitive enzyme immunoassay technique, to detect the presence of traces of soy in meat products. Specific rabbit polyclonal antiserum against soy protein was used as primary antibody. The optimal antigen concentration to be immobilized on the plate and the concentration of primary antibody to be used in competition was determined. The calibration curve was fitted using increasing concentrations of an extract of soy product. The soy product was extracted with Tris-HCl buffer 0.0625M with 3% sodium dodecylsulfate and 2% mercaptoethanol. The working range used in the enzyme immunoassay to detect soy was 9-280ppm SP with adequate linearity (R<sup>2</sup>: 0.9880). All validation parameters studied were appropriate. Commercial samples of meat products were analyzed with this enzyme immunoassays and a commercial ELISA kit. Significant differences were observed in the quantitative results obtained with both methods; nevertheless the developed enzyme immunoassay could be used as screening method.Facultad de Ciencias ExactasInstituto de Estudios Inmunológicos y Fisiopatológicos2017-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf57-62http://sedici.unlp.edu.ar/handle/10915/99255enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/57787info:eu-repo/semantics/altIdentifier/url/http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=154&doi=10.11648/j.jfns.20170502.16info:eu-repo/semantics/altIdentifier/issn/2330-7293info:eu-repo/semantics/altIdentifier/doi/10.11648/j.jfns.20170502.16info:eu-repo/semantics/altIdentifier/hdl/11336/57787info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:12:09Zoai:sedici.unlp.edu.ar:10915/99255Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:12:09.63SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Development of a Competitive Enzyme Immunoassay Technique for the Detection of Soy Traces in Meat Products
title Development of a Competitive Enzyme Immunoassay Technique for the Detection of Soy Traces in Meat Products
spellingShingle Development of a Competitive Enzyme Immunoassay Technique for the Detection of Soy Traces in Meat Products
Cellerino, Karina
Ciencias Exactas
Química
ELISA
Allergens
Soy detection
Meat products
title_short Development of a Competitive Enzyme Immunoassay Technique for the Detection of Soy Traces in Meat Products
title_full Development of a Competitive Enzyme Immunoassay Technique for the Detection of Soy Traces in Meat Products
title_fullStr Development of a Competitive Enzyme Immunoassay Technique for the Detection of Soy Traces in Meat Products
title_full_unstemmed Development of a Competitive Enzyme Immunoassay Technique for the Detection of Soy Traces in Meat Products
title_sort Development of a Competitive Enzyme Immunoassay Technique for the Detection of Soy Traces in Meat Products
dc.creator.none.fl_str_mv Cellerino, Karina
Rodríguez, Viviana Gladys
Docena, Guillermo Horacio
López, Laura Beatriz
author Cellerino, Karina
author_facet Cellerino, Karina
Rodríguez, Viviana Gladys
Docena, Guillermo Horacio
López, Laura Beatriz
author_role author
author2 Rodríguez, Viviana Gladys
Docena, Guillermo Horacio
López, Laura Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Química
ELISA
Allergens
Soy detection
Meat products
topic Ciencias Exactas
Química
ELISA
Allergens
Soy detection
Meat products
dc.description.none.fl_txt_mv The aim of this work was to develop a competitive enzyme immunoassay technique, to detect the presence of traces of soy in meat products. Specific rabbit polyclonal antiserum against soy protein was used as primary antibody. The optimal antigen concentration to be immobilized on the plate and the concentration of primary antibody to be used in competition was determined. The calibration curve was fitted using increasing concentrations of an extract of soy product. The soy product was extracted with Tris-HCl buffer 0.0625M with 3% sodium dodecylsulfate and 2% mercaptoethanol. The working range used in the enzyme immunoassay to detect soy was 9-280ppm SP with adequate linearity (R<sup>2</sup>: 0.9880). All validation parameters studied were appropriate. Commercial samples of meat products were analyzed with this enzyme immunoassays and a commercial ELISA kit. Significant differences were observed in the quantitative results obtained with both methods; nevertheless the developed enzyme immunoassay could be used as screening method.
Facultad de Ciencias Exactas
Instituto de Estudios Inmunológicos y Fisiopatológicos
description The aim of this work was to develop a competitive enzyme immunoassay technique, to detect the presence of traces of soy in meat products. Specific rabbit polyclonal antiserum against soy protein was used as primary antibody. The optimal antigen concentration to be immobilized on the plate and the concentration of primary antibody to be used in competition was determined. The calibration curve was fitted using increasing concentrations of an extract of soy product. The soy product was extracted with Tris-HCl buffer 0.0625M with 3% sodium dodecylsulfate and 2% mercaptoethanol. The working range used in the enzyme immunoassay to detect soy was 9-280ppm SP with adequate linearity (R<sup>2</sup>: 0.9880). All validation parameters studied were appropriate. Commercial samples of meat products were analyzed with this enzyme immunoassays and a commercial ELISA kit. Significant differences were observed in the quantitative results obtained with both methods; nevertheless the developed enzyme immunoassay could be used as screening method.
publishDate 2017
dc.date.none.fl_str_mv 2017-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/99255
url http://sedici.unlp.edu.ar/handle/10915/99255
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/57787
info:eu-repo/semantics/altIdentifier/url/http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=154&doi=10.11648/j.jfns.20170502.16
info:eu-repo/semantics/altIdentifier/issn/2330-7293
info:eu-repo/semantics/altIdentifier/doi/10.11648/j.jfns.20170502.16
info:eu-repo/semantics/altIdentifier/hdl/11336/57787
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
57-62
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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