Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits

Autores
Binaghi, Maria Julieta; Greco, Carola Beatriz; Martín, María Eugenia; Drago, Silvina Rosa; Ronayne de Ferrer, Patricia Ana; Lopez, Laura Beatriz
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Detection of milk traces was evaluated in cookies and extruded products model systems (MS) and in commercial products declaring milk presence through either the statement: “Contains…” or precautionary phrases. The aim of the study was to evaluate the performance of two different commercial ELISA kits for this purpose. Six cookies and extruded products model systems and two different batches of eight different types of commercial cookies and two types of extruded products were analyzed. The two different commercial ELISA kits used were R-Biopharm and Veratox-Neogen. In the cookies MS both kits had the same sensitivity while in the extruded products MS, the R- Biopharm kit had higher sensitivity than the Veratox-Neogen one. In the commercial samples only the snack declaring “Contains milk derivatives” showed detectable values of milk proteins. Even though all the remaining products included precautionary phrases on the label, none of them exceeded the quantification limit. The detection and quantification of the allergen will depend on the utilized kit and the treatment to which the ingredients have been submitted. Therefore, the performance of every kit should be studied for every allergen and every particular food matrix. Regarding commercial products, the analysis of more samples coming from different batches would be necessary in order to corroborate these results and verify the correct use of precautionary phrases.
Fil: Binaghi, Maria Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; Argentina
Fil: Greco, Carola Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; Argentina
Fil: Martín, María Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; Argentina
Fil: Drago, Silvina Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Ronayne de Ferrer, Patricia Ana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; Argentina
Fil: Lopez, Laura Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; Argentina
Materia
Allergens
Cookies
Extruded Products
Elisa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/70870
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/70870
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa KitsBinaghi, Maria JulietaGreco, Carola BeatrizMartín, María EugeniaDrago, Silvina RosaRonayne de Ferrer, Patricia AnaLopez, Laura BeatrizAllergensCookiesExtruded ProductsElisahttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Detection of milk traces was evaluated in cookies and extruded products model systems (MS) and in commercial products declaring milk presence through either the statement: “Contains…” or precautionary phrases. The aim of the study was to evaluate the performance of two different commercial ELISA kits for this purpose. Six cookies and extruded products model systems and two different batches of eight different types of commercial cookies and two types of extruded products were analyzed. The two different commercial ELISA kits used were R-Biopharm and Veratox-Neogen. In the cookies MS both kits had the same sensitivity while in the extruded products MS, the R- Biopharm kit had higher sensitivity than the Veratox-Neogen one. In the commercial samples only the snack declaring “Contains milk derivatives” showed detectable values of milk proteins. Even though all the remaining products included precautionary phrases on the label, none of them exceeded the quantification limit. The detection and quantification of the allergen will depend on the utilized kit and the treatment to which the ingredients have been submitted. Therefore, the performance of every kit should be studied for every allergen and every particular food matrix. Regarding commercial products, the analysis of more samples coming from different batches would be necessary in order to corroborate these results and verify the correct use of precautionary phrases.Fil: Binaghi, Maria Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; ArgentinaFil: Greco, Carola Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; ArgentinaFil: Martín, María Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; ArgentinaFil: Drago, Silvina Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Ronayne de Ferrer, Patricia Ana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; ArgentinaFil: Lopez, Laura Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; ArgentinaScience Publishing Group2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/70870Binaghi, Maria Julieta; Greco, Carola Beatriz; Martín, María Eugenia; Drago, Silvina Rosa; Ronayne de Ferrer, Patricia Ana; et al.; Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits; Science Publishing Group; Journal of Food and Nutrition Sciences; 4; 6; 11-2016; 175-1792330-72852330-7293CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.11648/j.jfns.20160406.16info:eu-repo/semantics/altIdentifier/url/http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=154&doi=10.11648/j.jfns.20160406.16info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:40Zoai:ri.conicet.gov.ar:11336/70870instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:40.657CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits
title Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits
spellingShingle Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits
Binaghi, Maria Julieta
Allergens
Cookies
Extruded Products
Elisa
title_short Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits
title_full Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits
title_fullStr Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits
title_full_unstemmed Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits
title_sort Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits
dc.creator.none.fl_str_mv Binaghi, Maria Julieta
Greco, Carola Beatriz
Martín, María Eugenia
Drago, Silvina Rosa
Ronayne de Ferrer, Patricia Ana
Lopez, Laura Beatriz
author Binaghi, Maria Julieta
author_facet Binaghi, Maria Julieta
Greco, Carola Beatriz
Martín, María Eugenia
Drago, Silvina Rosa
Ronayne de Ferrer, Patricia Ana
Lopez, Laura Beatriz
author_role author
author2 Greco, Carola Beatriz
Martín, María Eugenia
Drago, Silvina Rosa
Ronayne de Ferrer, Patricia Ana
Lopez, Laura Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Allergens
Cookies
Extruded Products
Elisa
topic Allergens
Cookies
Extruded Products
Elisa
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Detection of milk traces was evaluated in cookies and extruded products model systems (MS) and in commercial products declaring milk presence through either the statement: “Contains…” or precautionary phrases. The aim of the study was to evaluate the performance of two different commercial ELISA kits for this purpose. Six cookies and extruded products model systems and two different batches of eight different types of commercial cookies and two types of extruded products were analyzed. The two different commercial ELISA kits used were R-Biopharm and Veratox-Neogen. In the cookies MS both kits had the same sensitivity while in the extruded products MS, the R- Biopharm kit had higher sensitivity than the Veratox-Neogen one. In the commercial samples only the snack declaring “Contains milk derivatives” showed detectable values of milk proteins. Even though all the remaining products included precautionary phrases on the label, none of them exceeded the quantification limit. The detection and quantification of the allergen will depend on the utilized kit and the treatment to which the ingredients have been submitted. Therefore, the performance of every kit should be studied for every allergen and every particular food matrix. Regarding commercial products, the analysis of more samples coming from different batches would be necessary in order to corroborate these results and verify the correct use of precautionary phrases.
Fil: Binaghi, Maria Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; Argentina
Fil: Greco, Carola Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; Argentina
Fil: Martín, María Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; Argentina
Fil: Drago, Silvina Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Ronayne de Ferrer, Patricia Ana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; Argentina
Fil: Lopez, Laura Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad Nutrición Bromatología y Toxicología; Argentina
description Detection of milk traces was evaluated in cookies and extruded products model systems (MS) and in commercial products declaring milk presence through either the statement: “Contains…” or precautionary phrases. The aim of the study was to evaluate the performance of two different commercial ELISA kits for this purpose. Six cookies and extruded products model systems and two different batches of eight different types of commercial cookies and two types of extruded products were analyzed. The two different commercial ELISA kits used were R-Biopharm and Veratox-Neogen. In the cookies MS both kits had the same sensitivity while in the extruded products MS, the R- Biopharm kit had higher sensitivity than the Veratox-Neogen one. In the commercial samples only the snack declaring “Contains milk derivatives” showed detectable values of milk proteins. Even though all the remaining products included precautionary phrases on the label, none of them exceeded the quantification limit. The detection and quantification of the allergen will depend on the utilized kit and the treatment to which the ingredients have been submitted. Therefore, the performance of every kit should be studied for every allergen and every particular food matrix. Regarding commercial products, the analysis of more samples coming from different batches would be necessary in order to corroborate these results and verify the correct use of precautionary phrases.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/70870
Binaghi, Maria Julieta; Greco, Carola Beatriz; Martín, María Eugenia; Drago, Silvina Rosa; Ronayne de Ferrer, Patricia Ana; et al.; Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits; Science Publishing Group; Journal of Food and Nutrition Sciences; 4; 6; 11-2016; 175-179
2330-7285
2330-7293
CONICET Digital
CONICET
url http://hdl.handle.net/11336/70870
identifier_str_mv Binaghi, Maria Julieta; Greco, Carola Beatriz; Martín, María Eugenia; Drago, Silvina Rosa; Ronayne de Ferrer, Patricia Ana; et al.; Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits; Science Publishing Group; Journal of Food and Nutrition Sciences; 4; 6; 11-2016; 175-179
2330-7285
2330-7293
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.11648/j.jfns.20160406.16
info:eu-repo/semantics/altIdentifier/url/http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=154&doi=10.11648/j.jfns.20160406.16
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Science Publishing Group
publisher.none.fl_str_mv Science Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613457398202368
score 13.070432

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