<i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany
- Autores
- Moré, Gastón Andrés; Pantchev, A.; Skuballa, J.; Langenmayer, M. C.; Maksimov, Pavlo; Conraths, Franz Josef; Venturini, María Cecilia; Schares, Gereon
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Bovines are intermediate hosts of Sarcocystis cruzi, Sarcocystis hirsuta, and Sarcocystis hominis, which use canids, felids, or primates as definitive hosts, respectively. Cattle represent also intermediate hosts of Sarcocystis sinensis, but the definitive hosts of this parasite are not yet known. Sarcocystosis in cattle is frequently asymptomatic. The infection is characterized by the presence of thin-walled (S. cruzi) or thick-walled muscle cysts or sarcocysts (S. hominis, S. sinensis, and S. hirsuta). Recent reports suggest high prevalence of the zoonotic S. hominis in beef in Europe. We therefore aimed at differentiating Sarcocystis spp. in beef offered to consumers in Germany using molecular and microscopical methods, focusing on those species producing thick-walled sarcocysts. A total of 257 beef samples were obtained from different butcheries and supermarkets in Germany and processed by conventional and multiplex real-time PCR. In addition, 130 of these samples were processed by light microscopy and in 24.6 % thick-walled cysts were detected. Transmission electron microscopical analysis of six of these samples revealed an ultrastructural cyst wall pattern compatible with S. sinensis in five samples and with S. hominis in one sample. PCR-amplified 18S ribosomal DNA (rDNA) fragments of 28 individual thick-walled cysts were sequenced, and sequence identities of ≥98 % with S. sinensis (n = 22), S. hominis (n = 5) and S. hirsuta (n = 1) were observed. Moreover, nine Sarcocystis sp. 18S rDNA full length gene sequences were obtained, five of S. sinensis, three of S. hominis, and one of S. hirsuta. Out of all samples (n = 257), 174 (67.7 %) tested positive by conventional PCR and 179 (69.6 %) by multiplex real-time PCR for Sarcocystis spp. Regarding individual species, 134 (52 %), 95 (37 %), 17 (6.6 %), and 16 (6.2 %) were positive for S. cruzi, S. sinensis, S. hirsuta, and S. hominis, respectively. In conclusion, S. sinensis is the most prevalent thick-walled Sarcocystis species in beef offered for consumption in Germany. Further studies are needed to identify the final host of S. sinensis as well as the potential role of this protozoan as a differential diagnosis to the zoonotic species S. hominis.
Facultad de Ciencias Veterinarias
Laboratorio de Inmunoparasitología - Materia
-
Veterinaria
Sarcocystis spp.
Multiplex real-time PCR
Sequencing
Electron microscopy
Cattle
Beef - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/136422
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<i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in GermanyMoré, Gastón AndrésPantchev, A.Skuballa, J.Langenmayer, M. C.Maksimov, PavloConraths, Franz JosefVenturini, María CeciliaSchares, GereonVeterinariaSarcocystis spp.Multiplex real-time PCRSequencingElectron microscopyCattleBeefBovines are intermediate hosts of <i>Sarcocystis cruzi</i>, <i>Sarcocystis hirsuta</i>, and <i>Sarcocystis hominis</i>, which use canids, felids, or primates as definitive hosts, respectively. Cattle represent also intermediate hosts of <i>Sarcocystis sinensis</i>, but the definitive hosts of this parasite are not yet known. Sarcocystosis in cattle is frequently asymptomatic. The infection is characterized by the presence of thin-walled (<i>S. cruzi</i>) or thick-walled muscle cysts or sarcocysts (<i>S. hominis</i>, <i>S. sinensis</i>, and <i>S. hirsuta</i>). Recent reports suggest high prevalence of the zoonotic <i>S. hominis</i> in beef in Europe. We therefore aimed at differentiating Sarcocystis spp. in beef offered to consumers in Germany using molecular and microscopical methods, focusing on those species producing thick-walled sarcocysts. A total of 257 beef samples were obtained from different butcheries and supermarkets in Germany and processed by conventional and multiplex real-time PCR. In addition, 130 of these samples were processed by light microscopy and in 24.6 % thick-walled cysts were detected. Transmission electron microscopical analysis of six of these samples revealed an ultrastructural cyst wall pattern compatible with <i>S. sinensis</i> in five samples and with <i>S. hominis</i> in one sample. PCR-amplified 18S ribosomal DNA (rDNA) fragments of 28 individual thick-walled cysts were sequenced, and sequence identities of ≥98 % with <i>S. sinensis</i> (n = 22), <i>S. hominis</i> (n = 5) and <i>S. hirsuta</i> (n = 1) were observed. Moreover, nine Sarcocystis sp. 18S rDNA full length gene sequences were obtained, five of <i>S. sinensis</i>, three of <i>S. hominis</i>, and one of <i>S. hirsuta</i>. Out of all samples (n = 257), 174 (67.7 %) tested positive by conventional PCR and 179 (69.6 %) by multiplex real-time PCR for Sarcocystis spp. Regarding individual species, 134 (52 %), 95 (37 %), 17 (6.6 %), and 16 (6.2 %) were positive for <i>S. cruzi</i>, <i>S. sinensis</i>, <i>S. hirsuta</i>, and <i>S. hominis</i>, respectively. In conclusion, <i>S. sinensis</i> is the most prevalent thick-walled Sarcocystis species in beef offered for consumption in Germany. Further studies are needed to identify the final host of <i>S. sinensis</i> as well as the potential role of this protozoan as a differential diagnosis to the zoonotic species <i>S. hominis</i>.Facultad de Ciencias VeterinariasLaboratorio de Inmunoparasitología2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf2223-2230http://sedici.unlp.edu.ar/handle/10915/136422enginfo:eu-repo/semantics/altIdentifier/issn/1432-1955info:eu-repo/semantics/altIdentifier/issn/0932-0113info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-014-3877-xinfo:eu-repo/semantics/altIdentifier/pmid/24700022info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:58Zoai:sedici.unlp.edu.ar:10915/136422Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:58.41SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
<i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany |
title |
<i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany |
spellingShingle |
<i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany Moré, Gastón Andrés Veterinaria Sarcocystis spp. Multiplex real-time PCR Sequencing Electron microscopy Cattle Beef |
title_short |
<i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany |
title_full |
<i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany |
title_fullStr |
<i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany |
title_full_unstemmed |
<i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany |
title_sort |
<i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany |
dc.creator.none.fl_str_mv |
Moré, Gastón Andrés Pantchev, A. Skuballa, J. Langenmayer, M. C. Maksimov, Pavlo Conraths, Franz Josef Venturini, María Cecilia Schares, Gereon |
author |
Moré, Gastón Andrés |
author_facet |
Moré, Gastón Andrés Pantchev, A. Skuballa, J. Langenmayer, M. C. Maksimov, Pavlo Conraths, Franz Josef Venturini, María Cecilia Schares, Gereon |
author_role |
author |
author2 |
Pantchev, A. Skuballa, J. Langenmayer, M. C. Maksimov, Pavlo Conraths, Franz Josef Venturini, María Cecilia Schares, Gereon |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Veterinaria Sarcocystis spp. Multiplex real-time PCR Sequencing Electron microscopy Cattle Beef |
topic |
Veterinaria Sarcocystis spp. Multiplex real-time PCR Sequencing Electron microscopy Cattle Beef |
dc.description.none.fl_txt_mv |
Bovines are intermediate hosts of <i>Sarcocystis cruzi</i>, <i>Sarcocystis hirsuta</i>, and <i>Sarcocystis hominis</i>, which use canids, felids, or primates as definitive hosts, respectively. Cattle represent also intermediate hosts of <i>Sarcocystis sinensis</i>, but the definitive hosts of this parasite are not yet known. Sarcocystosis in cattle is frequently asymptomatic. The infection is characterized by the presence of thin-walled (<i>S. cruzi</i>) or thick-walled muscle cysts or sarcocysts (<i>S. hominis</i>, <i>S. sinensis</i>, and <i>S. hirsuta</i>). Recent reports suggest high prevalence of the zoonotic <i>S. hominis</i> in beef in Europe. We therefore aimed at differentiating Sarcocystis spp. in beef offered to consumers in Germany using molecular and microscopical methods, focusing on those species producing thick-walled sarcocysts. A total of 257 beef samples were obtained from different butcheries and supermarkets in Germany and processed by conventional and multiplex real-time PCR. In addition, 130 of these samples were processed by light microscopy and in 24.6 % thick-walled cysts were detected. Transmission electron microscopical analysis of six of these samples revealed an ultrastructural cyst wall pattern compatible with <i>S. sinensis</i> in five samples and with <i>S. hominis</i> in one sample. PCR-amplified 18S ribosomal DNA (rDNA) fragments of 28 individual thick-walled cysts were sequenced, and sequence identities of ≥98 % with <i>S. sinensis</i> (n = 22), <i>S. hominis</i> (n = 5) and <i>S. hirsuta</i> (n = 1) were observed. Moreover, nine Sarcocystis sp. 18S rDNA full length gene sequences were obtained, five of <i>S. sinensis</i>, three of <i>S. hominis</i>, and one of <i>S. hirsuta</i>. Out of all samples (n = 257), 174 (67.7 %) tested positive by conventional PCR and 179 (69.6 %) by multiplex real-time PCR for Sarcocystis spp. Regarding individual species, 134 (52 %), 95 (37 %), 17 (6.6 %), and 16 (6.2 %) were positive for <i>S. cruzi</i>, <i>S. sinensis</i>, <i>S. hirsuta</i>, and <i>S. hominis</i>, respectively. In conclusion, <i>S. sinensis</i> is the most prevalent thick-walled Sarcocystis species in beef offered for consumption in Germany. Further studies are needed to identify the final host of <i>S. sinensis</i> as well as the potential role of this protozoan as a differential diagnosis to the zoonotic species <i>S. hominis</i>. Facultad de Ciencias Veterinarias Laboratorio de Inmunoparasitología |
description |
Bovines are intermediate hosts of <i>Sarcocystis cruzi</i>, <i>Sarcocystis hirsuta</i>, and <i>Sarcocystis hominis</i>, which use canids, felids, or primates as definitive hosts, respectively. Cattle represent also intermediate hosts of <i>Sarcocystis sinensis</i>, but the definitive hosts of this parasite are not yet known. Sarcocystosis in cattle is frequently asymptomatic. The infection is characterized by the presence of thin-walled (<i>S. cruzi</i>) or thick-walled muscle cysts or sarcocysts (<i>S. hominis</i>, <i>S. sinensis</i>, and <i>S. hirsuta</i>). Recent reports suggest high prevalence of the zoonotic <i>S. hominis</i> in beef in Europe. We therefore aimed at differentiating Sarcocystis spp. in beef offered to consumers in Germany using molecular and microscopical methods, focusing on those species producing thick-walled sarcocysts. A total of 257 beef samples were obtained from different butcheries and supermarkets in Germany and processed by conventional and multiplex real-time PCR. In addition, 130 of these samples were processed by light microscopy and in 24.6 % thick-walled cysts were detected. Transmission electron microscopical analysis of six of these samples revealed an ultrastructural cyst wall pattern compatible with <i>S. sinensis</i> in five samples and with <i>S. hominis</i> in one sample. PCR-amplified 18S ribosomal DNA (rDNA) fragments of 28 individual thick-walled cysts were sequenced, and sequence identities of ≥98 % with <i>S. sinensis</i> (n = 22), <i>S. hominis</i> (n = 5) and <i>S. hirsuta</i> (n = 1) were observed. Moreover, nine Sarcocystis sp. 18S rDNA full length gene sequences were obtained, five of <i>S. sinensis</i>, three of <i>S. hominis</i>, and one of <i>S. hirsuta</i>. Out of all samples (n = 257), 174 (67.7 %) tested positive by conventional PCR and 179 (69.6 %) by multiplex real-time PCR for Sarcocystis spp. Regarding individual species, 134 (52 %), 95 (37 %), 17 (6.6 %), and 16 (6.2 %) were positive for <i>S. cruzi</i>, <i>S. sinensis</i>, <i>S. hirsuta</i>, and <i>S. hominis</i>, respectively. In conclusion, <i>S. sinensis</i> is the most prevalent thick-walled Sarcocystis species in beef offered for consumption in Germany. Further studies are needed to identify the final host of <i>S. sinensis</i> as well as the potential role of this protozoan as a differential diagnosis to the zoonotic species <i>S. hominis</i>. |
publishDate |
2014 |
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2014-06 |
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