<i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany

Autores
Moré, Gastón Andrés; Pantchev, A.; Skuballa, J.; Langenmayer, M. C.; Maksimov, Pavlo; Conraths, Franz Josef; Venturini, María Cecilia; Schares, Gereon
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovines are intermediate hosts of Sarcocystis cruzi, Sarcocystis hirsuta, and Sarcocystis hominis, which use canids, felids, or primates as definitive hosts, respectively. Cattle represent also intermediate hosts of Sarcocystis sinensis, but the definitive hosts of this parasite are not yet known. Sarcocystosis in cattle is frequently asymptomatic. The infection is characterized by the presence of thin-walled (S. cruzi) or thick-walled muscle cysts or sarcocysts (S. hominis, S. sinensis, and S. hirsuta). Recent reports suggest high prevalence of the zoonotic S. hominis in beef in Europe. We therefore aimed at differentiating Sarcocystis spp. in beef offered to consumers in Germany using molecular and microscopical methods, focusing on those species producing thick-walled sarcocysts. A total of 257 beef samples were obtained from different butcheries and supermarkets in Germany and processed by conventional and multiplex real-time PCR. In addition, 130 of these samples were processed by light microscopy and in 24.6 % thick-walled cysts were detected. Transmission electron microscopical analysis of six of these samples revealed an ultrastructural cyst wall pattern compatible with S. sinensis in five samples and with S. hominis in one sample. PCR-amplified 18S ribosomal DNA (rDNA) fragments of 28 individual thick-walled cysts were sequenced, and sequence identities of ≥98 % with S. sinensis (n = 22), S. hominis (n = 5) and S. hirsuta (n = 1) were observed. Moreover, nine Sarcocystis sp. 18S rDNA full length gene sequences were obtained, five of S. sinensis, three of S. hominis, and one of S. hirsuta. Out of all samples (n = 257), 174 (67.7 %) tested positive by conventional PCR and 179 (69.6 %) by multiplex real-time PCR for Sarcocystis spp. Regarding individual species, 134 (52 %), 95 (37 %), 17 (6.6 %), and 16 (6.2 %) were positive for S. cruzi, S. sinensis, S. hirsuta, and S. hominis, respectively. In conclusion, S. sinensis is the most prevalent thick-walled Sarcocystis species in beef offered for consumption in Germany. Further studies are needed to identify the final host of S. sinensis as well as the potential role of this protozoan as a differential diagnosis to the zoonotic species S. hominis.
Facultad de Ciencias Veterinarias
Laboratorio de Inmunoparasitología
Materia
Veterinaria
Sarcocystis spp.
Multiplex real-time PCR
Sequencing
Electron microscopy
Cattle
Beef
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/136422

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/136422
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network_name_str SEDICI (UNLP)
spelling <i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in GermanyMoré, Gastón AndrésPantchev, A.Skuballa, J.Langenmayer, M. C.Maksimov, PavloConraths, Franz JosefVenturini, María CeciliaSchares, GereonVeterinariaSarcocystis spp.Multiplex real-time PCRSequencingElectron microscopyCattleBeefBovines are intermediate hosts of <i>Sarcocystis cruzi</i>, <i>Sarcocystis hirsuta</i>, and <i>Sarcocystis hominis</i>, which use canids, felids, or primates as definitive hosts, respectively. Cattle represent also intermediate hosts of <i>Sarcocystis sinensis</i>, but the definitive hosts of this parasite are not yet known. Sarcocystosis in cattle is frequently asymptomatic. The infection is characterized by the presence of thin-walled (<i>S. cruzi</i>) or thick-walled muscle cysts or sarcocysts (<i>S. hominis</i>, <i>S. sinensis</i>, and <i>S. hirsuta</i>). Recent reports suggest high prevalence of the zoonotic <i>S. hominis</i> in beef in Europe. We therefore aimed at differentiating Sarcocystis spp. in beef offered to consumers in Germany using molecular and microscopical methods, focusing on those species producing thick-walled sarcocysts. A total of 257 beef samples were obtained from different butcheries and supermarkets in Germany and processed by conventional and multiplex real-time PCR. In addition, 130 of these samples were processed by light microscopy and in 24.6 % thick-walled cysts were detected. Transmission electron microscopical analysis of six of these samples revealed an ultrastructural cyst wall pattern compatible with <i>S. sinensis</i> in five samples and with <i>S. hominis</i> in one sample. PCR-amplified 18S ribosomal DNA (rDNA) fragments of 28 individual thick-walled cysts were sequenced, and sequence identities of ≥98 % with <i>S. sinensis</i> (n = 22), <i>S. hominis</i> (n = 5) and <i>S. hirsuta</i> (n = 1) were observed. Moreover, nine Sarcocystis sp. 18S rDNA full length gene sequences were obtained, five of <i>S. sinensis</i>, three of <i>S. hominis</i>, and one of <i>S. hirsuta</i>. Out of all samples (n = 257), 174 (67.7 %) tested positive by conventional PCR and 179 (69.6 %) by multiplex real-time PCR for Sarcocystis spp. Regarding individual species, 134 (52 %), 95 (37 %), 17 (6.6 %), and 16 (6.2 %) were positive for <i>S. cruzi</i>, <i>S. sinensis</i>, <i>S. hirsuta</i>, and <i>S. hominis</i>, respectively. In conclusion, <i>S. sinensis</i> is the most prevalent thick-walled Sarcocystis species in beef offered for consumption in Germany. Further studies are needed to identify the final host of <i>S. sinensis</i> as well as the potential role of this protozoan as a differential diagnosis to the zoonotic species <i>S. hominis</i>.Facultad de Ciencias VeterinariasLaboratorio de Inmunoparasitología2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf2223-2230http://sedici.unlp.edu.ar/handle/10915/136422enginfo:eu-repo/semantics/altIdentifier/issn/1432-1955info:eu-repo/semantics/altIdentifier/issn/0932-0113info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-014-3877-xinfo:eu-repo/semantics/altIdentifier/pmid/24700022info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:58Zoai:sedici.unlp.edu.ar:10915/136422Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:58.41SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv <i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany
title <i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany
spellingShingle <i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany
Moré, Gastón Andrés
Veterinaria
Sarcocystis spp.
Multiplex real-time PCR
Sequencing
Electron microscopy
Cattle
Beef
title_short <i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany
title_full <i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany
title_fullStr <i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany
title_full_unstemmed <i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany
title_sort <i>Sarcocystis sinensis</i> is the most prevalent thick-walled <i>Sarcocystis</i> species in beef on sale for consumers in Germany
dc.creator.none.fl_str_mv Moré, Gastón Andrés
Pantchev, A.
Skuballa, J.
Langenmayer, M. C.
Maksimov, Pavlo
Conraths, Franz Josef
Venturini, María Cecilia
Schares, Gereon
author Moré, Gastón Andrés
author_facet Moré, Gastón Andrés
Pantchev, A.
Skuballa, J.
Langenmayer, M. C.
Maksimov, Pavlo
Conraths, Franz Josef
Venturini, María Cecilia
Schares, Gereon
author_role author
author2 Pantchev, A.
Skuballa, J.
Langenmayer, M. C.
Maksimov, Pavlo
Conraths, Franz Josef
Venturini, María Cecilia
Schares, Gereon
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Veterinaria
Sarcocystis spp.
Multiplex real-time PCR
Sequencing
Electron microscopy
Cattle
Beef
topic Veterinaria
Sarcocystis spp.
Multiplex real-time PCR
Sequencing
Electron microscopy
Cattle
Beef
dc.description.none.fl_txt_mv Bovines are intermediate hosts of <i>Sarcocystis cruzi</i>, <i>Sarcocystis hirsuta</i>, and <i>Sarcocystis hominis</i>, which use canids, felids, or primates as definitive hosts, respectively. Cattle represent also intermediate hosts of <i>Sarcocystis sinensis</i>, but the definitive hosts of this parasite are not yet known. Sarcocystosis in cattle is frequently asymptomatic. The infection is characterized by the presence of thin-walled (<i>S. cruzi</i>) or thick-walled muscle cysts or sarcocysts (<i>S. hominis</i>, <i>S. sinensis</i>, and <i>S. hirsuta</i>). Recent reports suggest high prevalence of the zoonotic <i>S. hominis</i> in beef in Europe. We therefore aimed at differentiating Sarcocystis spp. in beef offered to consumers in Germany using molecular and microscopical methods, focusing on those species producing thick-walled sarcocysts. A total of 257 beef samples were obtained from different butcheries and supermarkets in Germany and processed by conventional and multiplex real-time PCR. In addition, 130 of these samples were processed by light microscopy and in 24.6 % thick-walled cysts were detected. Transmission electron microscopical analysis of six of these samples revealed an ultrastructural cyst wall pattern compatible with <i>S. sinensis</i> in five samples and with <i>S. hominis</i> in one sample. PCR-amplified 18S ribosomal DNA (rDNA) fragments of 28 individual thick-walled cysts were sequenced, and sequence identities of ≥98 % with <i>S. sinensis</i> (n = 22), <i>S. hominis</i> (n = 5) and <i>S. hirsuta</i> (n = 1) were observed. Moreover, nine Sarcocystis sp. 18S rDNA full length gene sequences were obtained, five of <i>S. sinensis</i>, three of <i>S. hominis</i>, and one of <i>S. hirsuta</i>. Out of all samples (n = 257), 174 (67.7 %) tested positive by conventional PCR and 179 (69.6 %) by multiplex real-time PCR for Sarcocystis spp. Regarding individual species, 134 (52 %), 95 (37 %), 17 (6.6 %), and 16 (6.2 %) were positive for <i>S. cruzi</i>, <i>S. sinensis</i>, <i>S. hirsuta</i>, and <i>S. hominis</i>, respectively. In conclusion, <i>S. sinensis</i> is the most prevalent thick-walled Sarcocystis species in beef offered for consumption in Germany. Further studies are needed to identify the final host of <i>S. sinensis</i> as well as the potential role of this protozoan as a differential diagnosis to the zoonotic species <i>S. hominis</i>.
Facultad de Ciencias Veterinarias
Laboratorio de Inmunoparasitología
description Bovines are intermediate hosts of <i>Sarcocystis cruzi</i>, <i>Sarcocystis hirsuta</i>, and <i>Sarcocystis hominis</i>, which use canids, felids, or primates as definitive hosts, respectively. Cattle represent also intermediate hosts of <i>Sarcocystis sinensis</i>, but the definitive hosts of this parasite are not yet known. Sarcocystosis in cattle is frequently asymptomatic. The infection is characterized by the presence of thin-walled (<i>S. cruzi</i>) or thick-walled muscle cysts or sarcocysts (<i>S. hominis</i>, <i>S. sinensis</i>, and <i>S. hirsuta</i>). Recent reports suggest high prevalence of the zoonotic <i>S. hominis</i> in beef in Europe. We therefore aimed at differentiating Sarcocystis spp. in beef offered to consumers in Germany using molecular and microscopical methods, focusing on those species producing thick-walled sarcocysts. A total of 257 beef samples were obtained from different butcheries and supermarkets in Germany and processed by conventional and multiplex real-time PCR. In addition, 130 of these samples were processed by light microscopy and in 24.6 % thick-walled cysts were detected. Transmission electron microscopical analysis of six of these samples revealed an ultrastructural cyst wall pattern compatible with <i>S. sinensis</i> in five samples and with <i>S. hominis</i> in one sample. PCR-amplified 18S ribosomal DNA (rDNA) fragments of 28 individual thick-walled cysts were sequenced, and sequence identities of ≥98 % with <i>S. sinensis</i> (n = 22), <i>S. hominis</i> (n = 5) and <i>S. hirsuta</i> (n = 1) were observed. Moreover, nine Sarcocystis sp. 18S rDNA full length gene sequences were obtained, five of <i>S. sinensis</i>, three of <i>S. hominis</i>, and one of <i>S. hirsuta</i>. Out of all samples (n = 257), 174 (67.7 %) tested positive by conventional PCR and 179 (69.6 %) by multiplex real-time PCR for Sarcocystis spp. Regarding individual species, 134 (52 %), 95 (37 %), 17 (6.6 %), and 16 (6.2 %) were positive for <i>S. cruzi</i>, <i>S. sinensis</i>, <i>S. hirsuta</i>, and <i>S. hominis</i>, respectively. In conclusion, <i>S. sinensis</i> is the most prevalent thick-walled Sarcocystis species in beef offered for consumption in Germany. Further studies are needed to identify the final host of <i>S. sinensis</i> as well as the potential role of this protozoan as a differential diagnosis to the zoonotic species <i>S. hominis</i>.
publishDate 2014
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