Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany.

Autores
Moré, Gastón Andrés; Pantchev, Nikola; Herrmann, Daland C.; Globokar Vrhovec, Majda; Öfner, Sabine; Conraths, Franz J.; Schares, Gereon
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sarcocystis spp. represent apicomplexan parasites. They usually have a heteroxenous life cycle. Around 200 species have been described, affecting a wide range of animals worldwide, including reptiles. In recent years, large numbers of reptiles have been imported into Europe as pets and, as a consequence, animal welfare and species protection issues emerged. A sample of pooled feces from four confiscated green pythons (Morelia viridis) containing Sarcocystis spp. sporocysts was
investigated. These snakes were imported for the pet trade and declared as being captive-bred. Full length 18S rRNA genes were amplified, cloned into plasmids and sequenced. Two different Sarcocystis spp. sequences were identified and registered as Sarcocystis sp. from M. viridis in GenBank. Both showed a 95?97% sequence identity with the 18S rRNA gene of Sarcocystis singaporensis. Phylogenetic analysis positioned these sequences together with other Sarcocystis spp. from snakesand rodents as definitive and intermediate hosts (IH), respectively. Sequence data and also the results of clinical and parasitological examinations suggest that the snakeswere definitive hosts for Sarcocystis spp. that circulate in wild IH. Thus, it seems unlikely that the infected snakes had been legally bred. Our research shows that information on the infection of snakes with Sarcocystis spp. may be used to assess compliance with regulations on the trade with wildlife species.
Fil: Moré, Gastón Andrés. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Pantchev, Nikola. IDEXX Vet Med Lab; Alemania
Fil: Herrmann, Daland C.. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania
Fil: Globokar Vrhovec, Majda. IDEXX Vet Med Lab; Alemania
Fil: Öfner, Sabine. Reptile Rescue Centre Munich; Alemania
Fil: Conraths, Franz J.. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania
Fil: Schares, Gereon. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania
Materia
Sarcocystis Spp.
18s Rrna Gene
Sequencing
Reptiles
Species Protection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31150
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany.Moré, Gastón AndrésPantchev, NikolaHerrmann, Daland C.Globokar Vrhovec, MajdaÖfner, SabineConraths, Franz J.Schares, GereonSarcocystis Spp.18s Rrna GeneSequencingReptilesSpecies Protectionhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Sarcocystis spp. represent apicomplexan parasites. They usually have a heteroxenous life cycle. Around 200 species have been described, affecting a wide range of animals worldwide, including reptiles. In recent years, large numbers of reptiles have been imported into Europe as pets and, as a consequence, animal welfare and species protection issues emerged. A sample of pooled feces from four confiscated green pythons (Morelia viridis) containing Sarcocystis spp. sporocysts was<br />investigated. These snakes were imported for the pet trade and declared as being captive-bred. Full length 18S rRNA genes were amplified, cloned into plasmids and sequenced. Two different Sarcocystis spp. sequences were identified and registered as Sarcocystis sp. from M. viridis in GenBank. Both showed a 95?97% sequence identity with the 18S rRNA gene of Sarcocystis singaporensis. Phylogenetic analysis positioned these sequences together with other Sarcocystis spp. from snakesand rodents as definitive and intermediate hosts (IH), respectively. Sequence data and also the results of clinical and parasitological examinations suggest that the snakeswere definitive hosts for Sarcocystis spp. that circulate in wild IH. Thus, it seems unlikely that the infected snakes had been legally bred. Our research shows that information on the infection of snakes with Sarcocystis spp. may be used to assess compliance with regulations on the trade with wildlife species.Fil: Moré, Gastón Andrés. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Pantchev, Nikola. IDEXX Vet Med Lab; AlemaniaFil: Herrmann, Daland C.. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; AlemaniaFil: Globokar Vrhovec, Majda. IDEXX Vet Med Lab; AlemaniaFil: Öfner, Sabine. Reptile Rescue Centre Munich; AlemaniaFil: Conraths, Franz J.. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; AlemaniaFil: Schares, Gereon. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; AlemaniaCambridge University Press2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31150Schares, Gereon; Conraths, Franz J.; Öfner, Sabine; Globokar Vrhovec, Majda; Herrmann, Daland C.; Pantchev, Nikola; et al.; Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany.; Cambridge University Press; Parasitology; 141; 5; 4-2014; 646-6510031-1820CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/molecular-identification-of-sarcocystis-spp-helped-to-define-the-origin-of-green-pythons-morelia-viridis-confiscated-in-germany/FD578CCDB01CA8342DC19B5ABD5E7529info:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182013001960info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:29Zoai:ri.conicet.gov.ar:11336/31150instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:30.178CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany.
title Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany.
spellingShingle Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany.
Moré, Gastón Andrés
Sarcocystis Spp.
18s Rrna Gene
Sequencing
Reptiles
Species Protection
title_short Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany.
title_full Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany.
title_fullStr Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany.
title_full_unstemmed Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany.
title_sort Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany.
dc.creator.none.fl_str_mv Moré, Gastón Andrés
Pantchev, Nikola
Herrmann, Daland C.
Globokar Vrhovec, Majda
Öfner, Sabine
Conraths, Franz J.
Schares, Gereon
author Moré, Gastón Andrés
author_facet Moré, Gastón Andrés
Pantchev, Nikola
Herrmann, Daland C.
Globokar Vrhovec, Majda
Öfner, Sabine
Conraths, Franz J.
Schares, Gereon
author_role author
author2 Pantchev, Nikola
Herrmann, Daland C.
Globokar Vrhovec, Majda
Öfner, Sabine
Conraths, Franz J.
Schares, Gereon
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Sarcocystis Spp.
18s Rrna Gene
Sequencing
Reptiles
Species Protection
topic Sarcocystis Spp.
18s Rrna Gene
Sequencing
Reptiles
Species Protection
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Sarcocystis spp. represent apicomplexan parasites. They usually have a heteroxenous life cycle. Around 200 species have been described, affecting a wide range of animals worldwide, including reptiles. In recent years, large numbers of reptiles have been imported into Europe as pets and, as a consequence, animal welfare and species protection issues emerged. A sample of pooled feces from four confiscated green pythons (Morelia viridis) containing Sarcocystis spp. sporocysts was<br />investigated. These snakes were imported for the pet trade and declared as being captive-bred. Full length 18S rRNA genes were amplified, cloned into plasmids and sequenced. Two different Sarcocystis spp. sequences were identified and registered as Sarcocystis sp. from M. viridis in GenBank. Both showed a 95?97% sequence identity with the 18S rRNA gene of Sarcocystis singaporensis. Phylogenetic analysis positioned these sequences together with other Sarcocystis spp. from snakesand rodents as definitive and intermediate hosts (IH), respectively. Sequence data and also the results of clinical and parasitological examinations suggest that the snakeswere definitive hosts for Sarcocystis spp. that circulate in wild IH. Thus, it seems unlikely that the infected snakes had been legally bred. Our research shows that information on the infection of snakes with Sarcocystis spp. may be used to assess compliance with regulations on the trade with wildlife species.
Fil: Moré, Gastón Andrés. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Pantchev, Nikola. IDEXX Vet Med Lab; Alemania
Fil: Herrmann, Daland C.. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania
Fil: Globokar Vrhovec, Majda. IDEXX Vet Med Lab; Alemania
Fil: Öfner, Sabine. Reptile Rescue Centre Munich; Alemania
Fil: Conraths, Franz J.. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania
Fil: Schares, Gereon. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania
description Sarcocystis spp. represent apicomplexan parasites. They usually have a heteroxenous life cycle. Around 200 species have been described, affecting a wide range of animals worldwide, including reptiles. In recent years, large numbers of reptiles have been imported into Europe as pets and, as a consequence, animal welfare and species protection issues emerged. A sample of pooled feces from four confiscated green pythons (Morelia viridis) containing Sarcocystis spp. sporocysts was<br />investigated. These snakes were imported for the pet trade and declared as being captive-bred. Full length 18S rRNA genes were amplified, cloned into plasmids and sequenced. Two different Sarcocystis spp. sequences were identified and registered as Sarcocystis sp. from M. viridis in GenBank. Both showed a 95?97% sequence identity with the 18S rRNA gene of Sarcocystis singaporensis. Phylogenetic analysis positioned these sequences together with other Sarcocystis spp. from snakesand rodents as definitive and intermediate hosts (IH), respectively. Sequence data and also the results of clinical and parasitological examinations suggest that the snakeswere definitive hosts for Sarcocystis spp. that circulate in wild IH. Thus, it seems unlikely that the infected snakes had been legally bred. Our research shows that information on the infection of snakes with Sarcocystis spp. may be used to assess compliance with regulations on the trade with wildlife species.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31150
Schares, Gereon; Conraths, Franz J.; Öfner, Sabine; Globokar Vrhovec, Majda; Herrmann, Daland C.; Pantchev, Nikola; et al.; Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany.; Cambridge University Press; Parasitology; 141; 5; 4-2014; 646-651
0031-1820
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31150
identifier_str_mv Schares, Gereon; Conraths, Franz J.; Öfner, Sabine; Globokar Vrhovec, Majda; Herrmann, Daland C.; Pantchev, Nikola; et al.; Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany.; Cambridge University Press; Parasitology; 141; 5; 4-2014; 646-651
0031-1820
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/molecular-identification-of-sarcocystis-spp-helped-to-define-the-origin-of-green-pythons-morelia-viridis-confiscated-in-germany/FD578CCDB01CA8342DC19B5ABD5E7529
info:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182013001960
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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