Intermediate filament proteins expression and carbohydrate moieties in trophoblast and decidual cells of mature cat placenta

Autores
Fernández, Patricia Elena; Diessler, Mónica Elizabeth; Pachamé, Andrea Viviana; Ortega, Hugo Hector; Gimeno, Eduardo Juan; Portiansky, Enrique Leo; Barbeito, Claudio Gustavo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to characterize cytoskeletal intermediate filament proteins and glycoconjugates of syncytiotrophoblast, cytotrophoblast and decidual cells of feline endotheliochorial placenta. Samples from 12 normal pregnant female cats, after 45 ± 5 days of gestation, were obtained removing the uterine horns by hysterectomy. Sections were processed for routine observation and for immunohistochemistry using anticytokeratin, antivimentin and antidesmin antibodies. In addition, lectin histochemistry was performed using a panel of several biotinylated lectins to characterize glycosides expression profile. Cytotrophoblast and syncytiotrophoblast showed immunoreactivity only with acidic and basic cytokeratins. Decidual cells were only positive to vimentin, consistent with their origin from endometrial fibroblasts. Trophoblast expressed a broad population of glycans, highly exposing terminal N-acetyl glucosamine residues and non-sialylated galactose and N-acetyl galactosamine oligomers. Oligosaccharides bound by Phaseolus vulgaris erythroagglutinin were the only highly branched N-linked residues evidenced in cats, and they were restricted to the syncytium. Unlike results reported on humans, mice and rats on lectin affinity of decidual cells, sialid acids and complex N-linked oligosaccharides were not demonstrated in cats. Glycosylation of proteins determines many of their final properties, thus becoming essential for the embryo-maternal dialogue during implantation and placentation. Changes in glycosylation pattern have been related to pathological pregnancies in other species. Hence, the knowledge about glycosylation profile of the normal cat placenta may lead to a better understanding of both normal and pathological reproductive events.
Fil: Fernández, Patricia Elena. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina
Fil: Diessler, Mónica Elizabeth. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pachamé, Andrea Viviana. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina
Fil: Portiansky, Enrique Leo. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Patologia; Argentina
Materia
Feline
Endotheliochorial Placentation
Cytotrophoblast Cells
Syncytiotrophoblast
Decidualization
Glycoconjugates
Cytokeratins
Vimentin
Desmin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20290
Acceder
id CONICETDig_8925eb8364f3d95928ef3cfbd72bbdd1
oai_identifier_str oai:ri.conicet.gov.ar:11336/20290
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Intermediate filament proteins expression and carbohydrate moieties in trophoblast and decidual cells of mature cat placentaFernández, Patricia ElenaDiessler, Mónica ElizabethPachamé, Andrea VivianaOrtega, Hugo HectorGimeno, Eduardo JuanPortiansky, Enrique LeoBarbeito, Claudio GustavoFelineEndotheliochorial PlacentationCytotrophoblast CellsSyncytiotrophoblastDecidualizationGlycoconjugatesCytokeratinsVimentinDesminhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to characterize cytoskeletal intermediate filament proteins and glycoconjugates of syncytiotrophoblast, cytotrophoblast and decidual cells of feline endotheliochorial placenta. Samples from 12 normal pregnant female cats, after 45 ± 5 days of gestation, were obtained removing the uterine horns by hysterectomy. Sections were processed for routine observation and for immunohistochemistry using anticytokeratin, antivimentin and antidesmin antibodies. In addition, lectin histochemistry was performed using a panel of several biotinylated lectins to characterize glycosides expression profile. Cytotrophoblast and syncytiotrophoblast showed immunoreactivity only with acidic and basic cytokeratins. Decidual cells were only positive to vimentin, consistent with their origin from endometrial fibroblasts. Trophoblast expressed a broad population of glycans, highly exposing terminal N-acetyl glucosamine residues and non-sialylated galactose and N-acetyl galactosamine oligomers. Oligosaccharides bound by Phaseolus vulgaris erythroagglutinin were the only highly branched N-linked residues evidenced in cats, and they were restricted to the syncytium. Unlike results reported on humans, mice and rats on lectin affinity of decidual cells, sialid acids and complex N-linked oligosaccharides were not demonstrated in cats. Glycosylation of proteins determines many of their final properties, thus becoming essential for the embryo-maternal dialogue during implantation and placentation. Changes in glycosylation pattern have been related to pathological pregnancies in other species. Hence, the knowledge about glycosylation profile of the normal cat placenta may lead to a better understanding of both normal and pathological reproductive events.Fil: Fernández, Patricia Elena. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; ArgentinaFil: Diessler, Mónica Elizabeth. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pachamé, Andrea Viviana. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; ArgentinaFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; ArgentinaFil: Portiansky, Enrique Leo. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Patologia; ArgentinaWiley2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20290Fernández, Patricia Elena; Diessler, Mónica Elizabeth; Pachamé, Andrea Viviana; Ortega, Hugo Hector; Gimeno, Eduardo Juan; et al.; Intermediate filament proteins expression and carbohydrate moieties in trophoblast and decidual cells of mature cat placenta; Wiley; Reproduction in Domestic Animals; 49; 2; 4-2014; 263-2690936-6768CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/rda.12265info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/rda.12265/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:47Zoai:ri.conicet.gov.ar:11336/20290instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:47.346CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intermediate filament proteins expression and carbohydrate moieties in trophoblast and decidual cells of mature cat placenta
title Intermediate filament proteins expression and carbohydrate moieties in trophoblast and decidual cells of mature cat placenta
spellingShingle Intermediate filament proteins expression and carbohydrate moieties in trophoblast and decidual cells of mature cat placenta
Fernández, Patricia Elena
Feline
Endotheliochorial Placentation
Cytotrophoblast Cells
Syncytiotrophoblast
Decidualization
Glycoconjugates
Cytokeratins
Vimentin
Desmin
title_short Intermediate filament proteins expression and carbohydrate moieties in trophoblast and decidual cells of mature cat placenta
title_full Intermediate filament proteins expression and carbohydrate moieties in trophoblast and decidual cells of mature cat placenta
title_fullStr Intermediate filament proteins expression and carbohydrate moieties in trophoblast and decidual cells of mature cat placenta
title_full_unstemmed Intermediate filament proteins expression and carbohydrate moieties in trophoblast and decidual cells of mature cat placenta
title_sort Intermediate filament proteins expression and carbohydrate moieties in trophoblast and decidual cells of mature cat placenta
dc.creator.none.fl_str_mv Fernández, Patricia Elena
Diessler, Mónica Elizabeth
Pachamé, Andrea Viviana
Ortega, Hugo Hector
Gimeno, Eduardo Juan
Portiansky, Enrique Leo
Barbeito, Claudio Gustavo
author Fernández, Patricia Elena
author_facet Fernández, Patricia Elena
Diessler, Mónica Elizabeth
Pachamé, Andrea Viviana
Ortega, Hugo Hector
Gimeno, Eduardo Juan
Portiansky, Enrique Leo
Barbeito, Claudio Gustavo
author_role author
author2 Diessler, Mónica Elizabeth
Pachamé, Andrea Viviana
Ortega, Hugo Hector
Gimeno, Eduardo Juan
Portiansky, Enrique Leo
Barbeito, Claudio Gustavo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Feline
Endotheliochorial Placentation
Cytotrophoblast Cells
Syncytiotrophoblast
Decidualization
Glycoconjugates
Cytokeratins
Vimentin
Desmin
topic Feline
Endotheliochorial Placentation
Cytotrophoblast Cells
Syncytiotrophoblast
Decidualization
Glycoconjugates
Cytokeratins
Vimentin
Desmin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this study was to characterize cytoskeletal intermediate filament proteins and glycoconjugates of syncytiotrophoblast, cytotrophoblast and decidual cells of feline endotheliochorial placenta. Samples from 12 normal pregnant female cats, after 45 ± 5 days of gestation, were obtained removing the uterine horns by hysterectomy. Sections were processed for routine observation and for immunohistochemistry using anticytokeratin, antivimentin and antidesmin antibodies. In addition, lectin histochemistry was performed using a panel of several biotinylated lectins to characterize glycosides expression profile. Cytotrophoblast and syncytiotrophoblast showed immunoreactivity only with acidic and basic cytokeratins. Decidual cells were only positive to vimentin, consistent with their origin from endometrial fibroblasts. Trophoblast expressed a broad population of glycans, highly exposing terminal N-acetyl glucosamine residues and non-sialylated galactose and N-acetyl galactosamine oligomers. Oligosaccharides bound by Phaseolus vulgaris erythroagglutinin were the only highly branched N-linked residues evidenced in cats, and they were restricted to the syncytium. Unlike results reported on humans, mice and rats on lectin affinity of decidual cells, sialid acids and complex N-linked oligosaccharides were not demonstrated in cats. Glycosylation of proteins determines many of their final properties, thus becoming essential for the embryo-maternal dialogue during implantation and placentation. Changes in glycosylation pattern have been related to pathological pregnancies in other species. Hence, the knowledge about glycosylation profile of the normal cat placenta may lead to a better understanding of both normal and pathological reproductive events.
Fil: Fernández, Patricia Elena. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina
Fil: Diessler, Mónica Elizabeth. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pachamé, Andrea Viviana. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina
Fil: Portiansky, Enrique Leo. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Patologia; Argentina
description The aim of this study was to characterize cytoskeletal intermediate filament proteins and glycoconjugates of syncytiotrophoblast, cytotrophoblast and decidual cells of feline endotheliochorial placenta. Samples from 12 normal pregnant female cats, after 45 ± 5 days of gestation, were obtained removing the uterine horns by hysterectomy. Sections were processed for routine observation and for immunohistochemistry using anticytokeratin, antivimentin and antidesmin antibodies. In addition, lectin histochemistry was performed using a panel of several biotinylated lectins to characterize glycosides expression profile. Cytotrophoblast and syncytiotrophoblast showed immunoreactivity only with acidic and basic cytokeratins. Decidual cells were only positive to vimentin, consistent with their origin from endometrial fibroblasts. Trophoblast expressed a broad population of glycans, highly exposing terminal N-acetyl glucosamine residues and non-sialylated galactose and N-acetyl galactosamine oligomers. Oligosaccharides bound by Phaseolus vulgaris erythroagglutinin were the only highly branched N-linked residues evidenced in cats, and they were restricted to the syncytium. Unlike results reported on humans, mice and rats on lectin affinity of decidual cells, sialid acids and complex N-linked oligosaccharides were not demonstrated in cats. Glycosylation of proteins determines many of their final properties, thus becoming essential for the embryo-maternal dialogue during implantation and placentation. Changes in glycosylation pattern have been related to pathological pregnancies in other species. Hence, the knowledge about glycosylation profile of the normal cat placenta may lead to a better understanding of both normal and pathological reproductive events.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20290
Fernández, Patricia Elena; Diessler, Mónica Elizabeth; Pachamé, Andrea Viviana; Ortega, Hugo Hector; Gimeno, Eduardo Juan; et al.; Intermediate filament proteins expression and carbohydrate moieties in trophoblast and decidual cells of mature cat placenta; Wiley; Reproduction in Domestic Animals; 49; 2; 4-2014; 263-269
0936-6768
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20290
identifier_str_mv Fernández, Patricia Elena; Diessler, Mónica Elizabeth; Pachamé, Andrea Viviana; Ortega, Hugo Hector; Gimeno, Eduardo Juan; et al.; Intermediate filament proteins expression and carbohydrate moieties in trophoblast and decidual cells of mature cat placenta; Wiley; Reproduction in Domestic Animals; 49; 2; 4-2014; 263-269
0936-6768
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/rda.12265
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/rda.12265/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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