Optimization and in vivo toxicity evaluation of G4.5 PAMAM dendrimer-risperidone complexes

Autores
Prieto, María Jimena; Rio Zabala, Nahuel Eduardo del; Marotta, Cristian Hernán; Carreño Gutierrez, Hector; Arevalo Arevalo, Rosario; Chiaramoni, Nadia Silvia; Alonso, Silvia del Valle
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Risperidone is an approved antipsychotic drug belonging to the chemical class of benzisoxazole. This drug has low solubility in aqueous medium and poor bioavailability due to extensive first-pass metabolism and high protein binding (.90%). Since new strategies to improve efficient treatments are needed, we studied the efficiency of anionic G4.5 PAMAM dendrimers as nanocarriers for this therapeutic drug. To this end, we explored dendrimer-risperidone complexation dependence on
solvent concentration, pH and molar relationship. The best dendrimer-risperidone incorporation (46 risperidone molecules per dendrimer) was achieved with a mixture of chloroform:methanol 50:50 v/v solution pH 3. In addition, to explore the possible effects of this complex,in vivo studies were carried out in the zebrafish model. Changes in the development of dopaminergic neurons and motoneurons were studied using tyrosine hydroxylase and calretinin, respectively. Physiological
changes were studied through histological sections stained with hematoxylin-eosin to observe possible morphological brain changes. The most significant changes were observed when larvae were treated with free risperidone, and no changes were observed when larvae were treated with the complex.
Instituto Multidisciplinario de Biología Celular
Materia
Biología
G4.5 PAMAM
In vivo toxicity
Dendrimer-risperidone complexes
Zebrafish morphological changes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/102625
Acceder
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network_name_str SEDICI (UNLP)
spelling Optimization and in vivo toxicity evaluation of G4.5 PAMAM dendrimer-risperidone complexesPrieto, María JimenaRio Zabala, Nahuel Eduardo delMarotta, Cristian HernánCarreño Gutierrez, HectorArevalo Arevalo, RosarioChiaramoni, Nadia SilviaAlonso, Silvia del ValleBiologíaG4.5 PAMAMIn vivo toxicityDendrimer-risperidone complexesZebrafish morphological changesRisperidone is an approved antipsychotic drug belonging to the chemical class of benzisoxazole. This drug has low solubility in aqueous medium and poor bioavailability due to extensive first-pass metabolism and high protein binding (.90%). Since new strategies to improve efficient treatments are needed, we studied the efficiency of anionic G4.5 PAMAM dendrimers as nanocarriers for this therapeutic drug. To this end, we explored dendrimer-risperidone complexation dependence on<br />solvent concentration, pH and molar relationship. The best dendrimer-risperidone incorporation (46 risperidone molecules per dendrimer) was achieved with a mixture of chloroform:methanol 50:50 v/v solution pH 3. In addition, to explore the possible effects of this complex,in vivo studies were carried out in the zebrafish model. Changes in the development of dopaminergic neurons and motoneurons were studied using tyrosine hydroxylase and calretinin, respectively. Physiological<br />changes were studied through histological sections stained with hematoxylin-eosin to observe possible morphological brain changes. The most significant changes were observed when larvae were treated with free risperidone, and no changes were observed when larvae were treated with the complex.Instituto Multidisciplinario de Biología Celular2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/102625enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/31250info:eu-repo/semantics/altIdentifier/issn/1932-6203info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0090393info:eu-repo/semantics/altIdentifier/hdl/11336/31250info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:11:55Zoai:sedici.unlp.edu.ar:10915/102625Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:11:55.852SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Optimization and in vivo toxicity evaluation of G4.5 PAMAM dendrimer-risperidone complexes
title Optimization and in vivo toxicity evaluation of G4.5 PAMAM dendrimer-risperidone complexes
spellingShingle Optimization and in vivo toxicity evaluation of G4.5 PAMAM dendrimer-risperidone complexes
Prieto, María Jimena
Biología
G4.5 PAMAM
In vivo toxicity
Dendrimer-risperidone complexes
Zebrafish morphological changes
title_short Optimization and in vivo toxicity evaluation of G4.5 PAMAM dendrimer-risperidone complexes
title_full Optimization and in vivo toxicity evaluation of G4.5 PAMAM dendrimer-risperidone complexes
title_fullStr Optimization and in vivo toxicity evaluation of G4.5 PAMAM dendrimer-risperidone complexes
title_full_unstemmed Optimization and in vivo toxicity evaluation of G4.5 PAMAM dendrimer-risperidone complexes
title_sort Optimization and in vivo toxicity evaluation of G4.5 PAMAM dendrimer-risperidone complexes
dc.creator.none.fl_str_mv Prieto, María Jimena
Rio Zabala, Nahuel Eduardo del
Marotta, Cristian Hernán
Carreño Gutierrez, Hector
Arevalo Arevalo, Rosario
Chiaramoni, Nadia Silvia
Alonso, Silvia del Valle
author Prieto, María Jimena
author_facet Prieto, María Jimena
Rio Zabala, Nahuel Eduardo del
Marotta, Cristian Hernán
Carreño Gutierrez, Hector
Arevalo Arevalo, Rosario
Chiaramoni, Nadia Silvia
Alonso, Silvia del Valle
author_role author
author2 Rio Zabala, Nahuel Eduardo del
Marotta, Cristian Hernán
Carreño Gutierrez, Hector
Arevalo Arevalo, Rosario
Chiaramoni, Nadia Silvia
Alonso, Silvia del Valle
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
G4.5 PAMAM
In vivo toxicity
Dendrimer-risperidone complexes
Zebrafish morphological changes
topic Biología
G4.5 PAMAM
In vivo toxicity
Dendrimer-risperidone complexes
Zebrafish morphological changes
dc.description.none.fl_txt_mv Risperidone is an approved antipsychotic drug belonging to the chemical class of benzisoxazole. This drug has low solubility in aqueous medium and poor bioavailability due to extensive first-pass metabolism and high protein binding (.90%). Since new strategies to improve efficient treatments are needed, we studied the efficiency of anionic G4.5 PAMAM dendrimers as nanocarriers for this therapeutic drug. To this end, we explored dendrimer-risperidone complexation dependence on<br />solvent concentration, pH and molar relationship. The best dendrimer-risperidone incorporation (46 risperidone molecules per dendrimer) was achieved with a mixture of chloroform:methanol 50:50 v/v solution pH 3. In addition, to explore the possible effects of this complex,in vivo studies were carried out in the zebrafish model. Changes in the development of dopaminergic neurons and motoneurons were studied using tyrosine hydroxylase and calretinin, respectively. Physiological<br />changes were studied through histological sections stained with hematoxylin-eosin to observe possible morphological brain changes. The most significant changes were observed when larvae were treated with free risperidone, and no changes were observed when larvae were treated with the complex.
Instituto Multidisciplinario de Biología Celular
description Risperidone is an approved antipsychotic drug belonging to the chemical class of benzisoxazole. This drug has low solubility in aqueous medium and poor bioavailability due to extensive first-pass metabolism and high protein binding (.90%). Since new strategies to improve efficient treatments are needed, we studied the efficiency of anionic G4.5 PAMAM dendrimers as nanocarriers for this therapeutic drug. To this end, we explored dendrimer-risperidone complexation dependence on<br />solvent concentration, pH and molar relationship. The best dendrimer-risperidone incorporation (46 risperidone molecules per dendrimer) was achieved with a mixture of chloroform:methanol 50:50 v/v solution pH 3. In addition, to explore the possible effects of this complex,in vivo studies were carried out in the zebrafish model. Changes in the development of dopaminergic neurons and motoneurons were studied using tyrosine hydroxylase and calretinin, respectively. Physiological<br />changes were studied through histological sections stained with hematoxylin-eosin to observe possible morphological brain changes. The most significant changes were observed when larvae were treated with free risperidone, and no changes were observed when larvae were treated with the complex.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/102625
url http://sedici.unlp.edu.ar/handle/10915/102625
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/31250
info:eu-repo/semantics/altIdentifier/issn/1932-6203
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0090393
info:eu-repo/semantics/altIdentifier/hdl/11336/31250
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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