In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea

Autores
Londero, Alejandra; Leotta, Gerardo Aníbal; Brusa, Victoria; Costa, Magdalena; Golijow, Carlos Daniel; Gutkind, Gabriel Osvaldo; Gonzalez, E.; Galli, Lucía
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, conventional culture methods for the isolation of diarrheal bacteria continue to be the most widely used form of diagnosis in many clinical laboratories. In this work we validated 11 in-house real-time polymerasechain reactions (PCRs) assays for the specific and rapid detection of Salmonella spp., Shigella spp., enteroinvasive E. coli, enteropathogenic E. coli, enterotoxigenic E. coli, Shiga toxin-producing E. coli, E. coli O157, Cronobacter sakazakii, Campylobacter jejuni, Campylobacter coli, Vibrio cholera and Clostridium difficile. The sensitivity of the assays was less than 100 CFU/ml for all the studied pathogens; selectivity and specificity were 100% in all cases and robustness was optimal. These PCR methods could be used to accurately detect the main bacterial causes of infant gastroenteritis.
Instituto de Genética Veterinaria
Materia
Bioquímica
Bacteria
Detection
Diarrheal
RT-PCR
Validation
Infant
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/100515
Acceder
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant DiarrheaLondero, AlejandraLeotta, Gerardo AníbalBrusa, VictoriaCosta, MagdalenaGolijow, Carlos DanielGutkind, Gabriel OsvaldoGonzalez, E.Galli, LucíaBioquímicaBacteriaDetectionDiarrhealRT-PCRValidationInfantIn Argentina, conventional culture methods for the isolation of diarrheal bacteria continue to be the most widely used form of diagnosis in many clinical laboratories. In this work we validated 11 in-house real-time polymerasechain reactions (PCRs) assays for the specific and rapid detection of Salmonella spp., Shigella spp., enteroinvasive E. coli, enteropathogenic E. coli, enterotoxigenic E. coli, Shiga toxin-producing E. coli, E. coli O157, Cronobacter sakazakii, Campylobacter jejuni, Campylobacter coli, Vibrio cholera and Clostridium difficile. The sensitivity of the assays was less than 100 CFU/ml for all the studied pathogens; selectivity and specificity were 100% in all cases and robustness was optimal. These PCR methods could be used to accurately detect the main bacterial causes of infant gastroenteritis.Instituto de Genética Veterinaria2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf268-271http://sedici.unlp.edu.ar/handle/10915/100515enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/48103info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/inhouse-validation-of-rapid-detection-pcrs-for-bacterial-pathogens-causing-infant-diarrhea-2161-0703-1000268-97066.htmlinfo:eu-repo/semantics/altIdentifier/url/https://www.hilarispublisher.com/open-access/inhouse-validation-of-rapid-detection-pcrs-for-bacterial-pathogens-causinginfant-diarrhea-2161-0703-1000268.pdfinfo:eu-repo/semantics/altIdentifier/issn/2161-0703info:eu-repo/semantics/altIdentifier/doi/10.4172/2161-0703.1000268info:eu-repo/semantics/altIdentifier/hdl/11336/48103info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:20:05Zoai:sedici.unlp.edu.ar:10915/100515Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:20:06.074SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea
title In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea
spellingShingle In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea
Londero, Alejandra
Bioquímica
Bacteria
Detection
Diarrheal
RT-PCR
Validation
Infant
title_short In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea
title_full In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea
title_fullStr In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea
title_full_unstemmed In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea
title_sort In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea
dc.creator.none.fl_str_mv Londero, Alejandra
Leotta, Gerardo Aníbal
Brusa, Victoria
Costa, Magdalena
Golijow, Carlos Daniel
Gutkind, Gabriel Osvaldo
Gonzalez, E.
Galli, Lucía
author Londero, Alejandra
author_facet Londero, Alejandra
Leotta, Gerardo Aníbal
Brusa, Victoria
Costa, Magdalena
Golijow, Carlos Daniel
Gutkind, Gabriel Osvaldo
Gonzalez, E.
Galli, Lucía
author_role author
author2 Leotta, Gerardo Aníbal
Brusa, Victoria
Costa, Magdalena
Golijow, Carlos Daniel
Gutkind, Gabriel Osvaldo
Gonzalez, E.
Galli, Lucía
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
Bacteria
Detection
Diarrheal
RT-PCR
Validation
Infant
topic Bioquímica
Bacteria
Detection
Diarrheal
RT-PCR
Validation
Infant
dc.description.none.fl_txt_mv In Argentina, conventional culture methods for the isolation of diarrheal bacteria continue to be the most widely used form of diagnosis in many clinical laboratories. In this work we validated 11 in-house real-time polymerasechain reactions (PCRs) assays for the specific and rapid detection of Salmonella spp., Shigella spp., enteroinvasive E. coli, enteropathogenic E. coli, enterotoxigenic E. coli, Shiga toxin-producing E. coli, E. coli O157, Cronobacter sakazakii, Campylobacter jejuni, Campylobacter coli, Vibrio cholera and Clostridium difficile. The sensitivity of the assays was less than 100 CFU/ml for all the studied pathogens; selectivity and specificity were 100% in all cases and robustness was optimal. These PCR methods could be used to accurately detect the main bacterial causes of infant gastroenteritis.
Instituto de Genética Veterinaria
description In Argentina, conventional culture methods for the isolation of diarrheal bacteria continue to be the most widely used form of diagnosis in many clinical laboratories. In this work we validated 11 in-house real-time polymerasechain reactions (PCRs) assays for the specific and rapid detection of Salmonella spp., Shigella spp., enteroinvasive E. coli, enteropathogenic E. coli, enterotoxigenic E. coli, Shiga toxin-producing E. coli, E. coli O157, Cronobacter sakazakii, Campylobacter jejuni, Campylobacter coli, Vibrio cholera and Clostridium difficile. The sensitivity of the assays was less than 100 CFU/ml for all the studied pathogens; selectivity and specificity were 100% in all cases and robustness was optimal. These PCR methods could be used to accurately detect the main bacterial causes of infant gastroenteritis.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/100515
url http://sedici.unlp.edu.ar/handle/10915/100515
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/48103
info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/inhouse-validation-of-rapid-detection-pcrs-for-bacterial-pathogens-causing-infant-diarrhea-2161-0703-1000268-97066.html
info:eu-repo/semantics/altIdentifier/url/https://www.hilarispublisher.com/open-access/inhouse-validation-of-rapid-detection-pcrs-for-bacterial-pathogens-causinginfant-diarrhea-2161-0703-1000268.pdf
info:eu-repo/semantics/altIdentifier/issn/2161-0703
info:eu-repo/semantics/altIdentifier/doi/10.4172/2161-0703.1000268
info:eu-repo/semantics/altIdentifier/hdl/11336/48103
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
268-271
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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