Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene

Autores
Dewey, Ricardo Alfredo; Semorile, Liliana Carmen; Crisci, Jorge Víctor; Grau, Oscar
Año de publicación
1996
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and similarity were made. A partial sequence of the N gene was able to classify our local isolates within three Tospovirus species previously described (Tomato spotted wilt virus, TSWV; tomato chlorotic spot virus, TCSV and groundnut ringspot virus, GRSV). With the sequence data currently available, we performed a cladistic phylogenetic analysis which gave a possible genealogy among members of the Tospovirus genus.
Instituto de Biotecnología y Biología Molecular
Facultad de Ciencias Naturales y Museo
Materia
Bioquímica
Tospovirus
N gene
RT-PCR
Cladistics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/131981
Acceder
id SEDICI_66a787c48a95864fbce61d571e3a443f
oai_identifier_str oai:sedici.unlp.edu.ar:10915/131981
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N geneDewey, Ricardo AlfredoSemorile, Liliana CarmenCrisci, Jorge VíctorGrau, OscarBioquímicaTospovirusN geneRT-PCRCladisticsThe genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and similarity were made. A partial sequence of the N gene was able to classify our local isolates within three Tospovirus species previously described (Tomato spotted wilt virus, TSWV; tomato chlorotic spot virus, TCSV and groundnut ringspot virus, GRSV). With the sequence data currently available, we performed a cladistic phylogenetic analysis which gave a possible genealogy among members of the Tospovirus genus.Instituto de Biotecnología y Biología MolecularFacultad de Ciencias Naturales y Museo1996info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf255-262http://sedici.unlp.edu.ar/handle/10915/131981enginfo:eu-repo/semantics/altIdentifier/issn/0920-8569info:eu-repo/semantics/altIdentifier/issn/1572-994Xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/bf00366986info:eu-repo/semantics/altIdentifier/pmid/9035370info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-10T12:35:10Zoai:sedici.unlp.edu.ar:10915/131981Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-10 12:35:11.069SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene
title Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene
spellingShingle Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene
Dewey, Ricardo Alfredo
Bioquímica
Tospovirus
N gene
RT-PCR
Cladistics
title_short Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene
title_full Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene
title_fullStr Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene
title_full_unstemmed Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene
title_sort Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene
dc.creator.none.fl_str_mv Dewey, Ricardo Alfredo
Semorile, Liliana Carmen
Crisci, Jorge Víctor
Grau, Oscar
author Dewey, Ricardo Alfredo
author_facet Dewey, Ricardo Alfredo
Semorile, Liliana Carmen
Crisci, Jorge Víctor
Grau, Oscar
author_role author
author2 Semorile, Liliana Carmen
Crisci, Jorge Víctor
Grau, Oscar
author2_role author
author
author
dc.subject.none.fl_str_mv Bioquímica
Tospovirus
N gene
RT-PCR
Cladistics
topic Bioquímica
Tospovirus
N gene
RT-PCR
Cladistics
dc.description.none.fl_txt_mv The genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and similarity were made. A partial sequence of the N gene was able to classify our local isolates within three Tospovirus species previously described (Tomato spotted wilt virus, TSWV; tomato chlorotic spot virus, TCSV and groundnut ringspot virus, GRSV). With the sequence data currently available, we performed a cladistic phylogenetic analysis which gave a possible genealogy among members of the Tospovirus genus.
Instituto de Biotecnología y Biología Molecular
Facultad de Ciencias Naturales y Museo
description The genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and similarity were made. A partial sequence of the N gene was able to classify our local isolates within three Tospovirus species previously described (Tomato spotted wilt virus, TSWV; tomato chlorotic spot virus, TCSV and groundnut ringspot virus, GRSV). With the sequence data currently available, we performed a cladistic phylogenetic analysis which gave a possible genealogy among members of the Tospovirus genus.
publishDate 1996
dc.date.none.fl_str_mv 1996
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/131981
url http://sedici.unlp.edu.ar/handle/10915/131981
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0920-8569
info:eu-repo/semantics/altIdentifier/issn/1572-994X
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00366986
info:eu-repo/semantics/altIdentifier/pmid/9035370
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
255-262
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1842904491357110272
score 12.993085

Publicaciones similares