Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate

Autores
Chesini, Mariana; Neila, Lorena Paola; Fratebianchi de la Parra, Dante; Rojas, Natalia Lorena; Contreras Esquivel, Juan Carlos; Cavalitto, Sebastián Fernando; Ghiringhelli, Pablo Daniel; Hours, Roque Alberto
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Inulinases have been extracted and characterized from inulin-storing tissues; however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as inulinase producers. These hydrolases are usually inducible and their exo-acting forms may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose and raffinose. Fungal inulinase extracts are often produced as stable mixture of highly active fructanhydrolases. From a practical prospective, the best known inulinases to date are those produced by species of Penicillium, Aspergillus and Kluyveromyces. Results: The production of extracellular inulinase by A. kawachii in liquid cultures, using either inulin or yacon derived materials as CES as well as inulinase inducers, is reported. In addition, a partial characterization of the enzyme activity is included. Conclusions: Yacon derived products, particularly yacon juice, added to the culture medium proved to be a good CES for fungal growth as well as an inducer of enzyme synthesis. Partial characterization of the enzyme revealed that it is quite stable in a wide range of pH and temperature. In addition, characterization of the reaction products revealed that this enzyme corresponds to an exo-type. These facts are promising considering its potential application in inulin hydrolysis for the production of high fructose syrups.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
Acidophilic enzymes
Batch cultures
Inulin degradation
Submerged cultures
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/85397

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spelling Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrateChesini, MarianaNeila, Lorena PaolaFratebianchi de la Parra, DanteRojas, Natalia LorenaContreras Esquivel, Juan CarlosCavalitto, Sebastián FernandoGhiringhelli, Pablo DanielHours, Roque AlbertoCiencias ExactasAcidophilic enzymesBatch culturesInulin degradationSubmerged culturesBackground: Inulinases have been extracted and characterized from inulin-storing tissues; however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as inulinase producers. These hydrolases are usually inducible and their exo-acting forms may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose and raffinose. Fungal inulinase extracts are often produced as stable mixture of highly active fructanhydrolases. From a practical prospective, the best known inulinases to date are those produced by species of Penicillium, Aspergillus and Kluyveromyces. Results: The production of extracellular inulinase by A. kawachii in liquid cultures, using either inulin or yacon derived materials as CES as well as inulinase inducers, is reported. In addition, a partial characterization of the enzyme activity is included. Conclusions: Yacon derived products, particularly yacon juice, added to the culture medium proved to be a good CES for fungal growth as well as an inducer of enzyme synthesis. Partial characterization of the enzyme revealed that it is quite stable in a wide range of pH and temperature. In addition, characterization of the reaction products revealed that this enzyme corresponds to an exo-type. These facts are promising considering its potential application in inulin hydrolysis for the production of high fructose syrups.Centro de Investigación y Desarrollo en Fermentaciones Industriales2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/85397enginfo:eu-repo/semantics/altIdentifier/issn/0717-3458info:eu-repo/semantics/altIdentifier/doi/10.2225/vol16-issue3-fulltext-13info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:30Zoai:sedici.unlp.edu.ar:10915/85397Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:31.01SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
spellingShingle Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
Chesini, Mariana
Ciencias Exactas
Acidophilic enzymes
Batch cultures
Inulin degradation
Submerged cultures
title_short Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title_full Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title_fullStr Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title_full_unstemmed Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title_sort Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
dc.creator.none.fl_str_mv Chesini, Mariana
Neila, Lorena Paola
Fratebianchi de la Parra, Dante
Rojas, Natalia Lorena
Contreras Esquivel, Juan Carlos
Cavalitto, Sebastián Fernando
Ghiringhelli, Pablo Daniel
Hours, Roque Alberto
author Chesini, Mariana
author_facet Chesini, Mariana
Neila, Lorena Paola
Fratebianchi de la Parra, Dante
Rojas, Natalia Lorena
Contreras Esquivel, Juan Carlos
Cavalitto, Sebastián Fernando
Ghiringhelli, Pablo Daniel
Hours, Roque Alberto
author_role author
author2 Neila, Lorena Paola
Fratebianchi de la Parra, Dante
Rojas, Natalia Lorena
Contreras Esquivel, Juan Carlos
Cavalitto, Sebastián Fernando
Ghiringhelli, Pablo Daniel
Hours, Roque Alberto
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Acidophilic enzymes
Batch cultures
Inulin degradation
Submerged cultures
topic Ciencias Exactas
Acidophilic enzymes
Batch cultures
Inulin degradation
Submerged cultures
dc.description.none.fl_txt_mv Background: Inulinases have been extracted and characterized from inulin-storing tissues; however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as inulinase producers. These hydrolases are usually inducible and their exo-acting forms may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose and raffinose. Fungal inulinase extracts are often produced as stable mixture of highly active fructanhydrolases. From a practical prospective, the best known inulinases to date are those produced by species of Penicillium, Aspergillus and Kluyveromyces. Results: The production of extracellular inulinase by A. kawachii in liquid cultures, using either inulin or yacon derived materials as CES as well as inulinase inducers, is reported. In addition, a partial characterization of the enzyme activity is included. Conclusions: Yacon derived products, particularly yacon juice, added to the culture medium proved to be a good CES for fungal growth as well as an inducer of enzyme synthesis. Partial characterization of the enzyme revealed that it is quite stable in a wide range of pH and temperature. In addition, characterization of the reaction products revealed that this enzyme corresponds to an exo-type. These facts are promising considering its potential application in inulin hydrolysis for the production of high fructose syrups.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description Background: Inulinases have been extracted and characterized from inulin-storing tissues; however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as inulinase producers. These hydrolases are usually inducible and their exo-acting forms may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose and raffinose. Fungal inulinase extracts are often produced as stable mixture of highly active fructanhydrolases. From a practical prospective, the best known inulinases to date are those produced by species of Penicillium, Aspergillus and Kluyveromyces. Results: The production of extracellular inulinase by A. kawachii in liquid cultures, using either inulin or yacon derived materials as CES as well as inulinase inducers, is reported. In addition, a partial characterization of the enzyme activity is included. Conclusions: Yacon derived products, particularly yacon juice, added to the culture medium proved to be a good CES for fungal growth as well as an inducer of enzyme synthesis. Partial characterization of the enzyme revealed that it is quite stable in a wide range of pH and temperature. In addition, characterization of the reaction products revealed that this enzyme corresponds to an exo-type. These facts are promising considering its potential application in inulin hydrolysis for the production of high fructose syrups.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/85397
url http://sedici.unlp.edu.ar/handle/10915/85397
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0717-3458
info:eu-repo/semantics/altIdentifier/doi/10.2225/vol16-issue3-fulltext-13
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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