Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate

Autores
Ghiringhelli, Pablo Daniel; Hours, Roque Alberto; Chesini, Mariana; Neila, Lorena Paola; Fratebianchi de la Parra, Dante; Rojas, Natalia Lorena; Contreras Esquivel, Juan Carlos; Cavalitto, Sebastian Fernando
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Inulinases have been extracted and characterized from inulin-storing tissues,however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as inulinase producers. These hydrolases are usually inducible and their exo-acting forms may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose and raffinose. Fungal inulinase extracts are often produced as stable mixture of highly active fructanhydrolases. From a practical prospective, the best known inulinases to date are those produced by species of Penicillium, Aspergillus and Kluyveromyces. Results: The production of extracellular inulinase by A. kawachii in liquid cultures, using either inulin or yacon derived materials as CES as well as inulinase inducers, is reported. In addition, a partial characterization of the enzyme activity is included. Conclusions: Yacon derived products, particularly yacon juice, added to the culture medium proved to be a good CES for fungal growth as well as an inducer of enzyme synthesis. Partial characterization of the enzyme revealed that it is quite stable in a wide range of pH and temperature. In addition, characterization of the reaction products revealed that this enzyme corresponds to an exo-type. These facts are promising considering its potential application in inulin hydrolysis for the production of high fructose syrups.
Fil: Ghiringhelli, Pablo Daniel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina
Fil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Chesini, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Neila, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Fratebianchi de la Parra, Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Rojas, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Contreras Esquivel, Juan Carlos. Universidad Autónoma de Coahuila, Facultad de Química; México
Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Materia
ACIDOPHILIC ENZYMES
BATCH CULTURES
INULIN DEGRADATION
SUBMERGED CULTURES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3385
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/3385
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network_name_str CONICET Digital (CONICET)
spelling Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrateGhiringhelli, Pablo DanielHours, Roque AlbertoChesini, MarianaNeila, Lorena PaolaFratebianchi de la Parra, DanteRojas, Natalia LorenaContreras Esquivel, Juan CarlosCavalitto, Sebastian FernandoACIDOPHILIC ENZYMESBATCH CULTURESINULIN DEGRADATIONSUBMERGED CULTUREShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Inulinases have been extracted and characterized from inulin-storing tissues,however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as inulinase producers. These hydrolases are usually inducible and their exo-acting forms may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose and raffinose. Fungal inulinase extracts are often produced as stable mixture of highly active fructanhydrolases. From a practical prospective, the best known inulinases to date are those produced by species of Penicillium, Aspergillus and Kluyveromyces. Results: The production of extracellular inulinase by A. kawachii in liquid cultures, using either inulin or yacon derived materials as CES as well as inulinase inducers, is reported. In addition, a partial characterization of the enzyme activity is included. Conclusions: Yacon derived products, particularly yacon juice, added to the culture medium proved to be a good CES for fungal growth as well as an inducer of enzyme synthesis. Partial characterization of the enzyme revealed that it is quite stable in a wide range of pH and temperature. In addition, characterization of the reaction products revealed that this enzyme corresponds to an exo-type. These facts are promising considering its potential application in inulin hydrolysis for the production of high fructose syrups.Fil: Ghiringhelli, Pablo Daniel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; ArgentinaFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Chesini, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Neila, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Fratebianchi de la Parra, Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Rojas, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Contreras Esquivel, Juan Carlos. Universidad Autónoma de Coahuila, Facultad de Química; MéxicoFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaPontificia Universidad Catolica de Valparaíso2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3385Ghiringhelli, Pablo Daniel; Hours, Roque Alberto; Chesini, Mariana; Neila, Lorena Paola; Fratebianchi de la Parra, Dante; et al.; Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate; Pontificia Universidad Catolica de Valparaíso; Electronic Journal Of Biotechnology; 16; 3; 5-2013; 1-130717-3458enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/4h9fbxinfo:eu-repo/semantics/altIdentifier/doi/10.2225/vol16-issue3-fulltext-13info:eu-repo/semantics/altIdentifier/url/www.ejbiotechnology.info/index.php/ejbiotechnology/rt/printerFriendly/v16n3-13/1613info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)https://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:58Zoai:ri.conicet.gov.ar:11336/3385instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:59.013CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
spellingShingle Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
Ghiringhelli, Pablo Daniel
ACIDOPHILIC ENZYMES
BATCH CULTURES
INULIN DEGRADATION
SUBMERGED CULTURES
title_short Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title_full Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title_fullStr Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title_full_unstemmed Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title_sort Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
dc.creator.none.fl_str_mv Ghiringhelli, Pablo Daniel
Hours, Roque Alberto
Chesini, Mariana
Neila, Lorena Paola
Fratebianchi de la Parra, Dante
Rojas, Natalia Lorena
Contreras Esquivel, Juan Carlos
Cavalitto, Sebastian Fernando
author Ghiringhelli, Pablo Daniel
author_facet Ghiringhelli, Pablo Daniel
Hours, Roque Alberto
Chesini, Mariana
Neila, Lorena Paola
Fratebianchi de la Parra, Dante
Rojas, Natalia Lorena
Contreras Esquivel, Juan Carlos
Cavalitto, Sebastian Fernando
author_role author
author2 Hours, Roque Alberto
Chesini, Mariana
Neila, Lorena Paola
Fratebianchi de la Parra, Dante
Rojas, Natalia Lorena
Contreras Esquivel, Juan Carlos
Cavalitto, Sebastian Fernando
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACIDOPHILIC ENZYMES
BATCH CULTURES
INULIN DEGRADATION
SUBMERGED CULTURES
topic ACIDOPHILIC ENZYMES
BATCH CULTURES
INULIN DEGRADATION
SUBMERGED CULTURES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Inulinases have been extracted and characterized from inulin-storing tissues,however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as inulinase producers. These hydrolases are usually inducible and their exo-acting forms may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose and raffinose. Fungal inulinase extracts are often produced as stable mixture of highly active fructanhydrolases. From a practical prospective, the best known inulinases to date are those produced by species of Penicillium, Aspergillus and Kluyveromyces. Results: The production of extracellular inulinase by A. kawachii in liquid cultures, using either inulin or yacon derived materials as CES as well as inulinase inducers, is reported. In addition, a partial characterization of the enzyme activity is included. Conclusions: Yacon derived products, particularly yacon juice, added to the culture medium proved to be a good CES for fungal growth as well as an inducer of enzyme synthesis. Partial characterization of the enzyme revealed that it is quite stable in a wide range of pH and temperature. In addition, characterization of the reaction products revealed that this enzyme corresponds to an exo-type. These facts are promising considering its potential application in inulin hydrolysis for the production of high fructose syrups.
Fil: Ghiringhelli, Pablo Daniel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina
Fil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Chesini, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Neila, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Fratebianchi de la Parra, Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Rojas, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Contreras Esquivel, Juan Carlos. Universidad Autónoma de Coahuila, Facultad de Química; México
Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
description Inulinases have been extracted and characterized from inulin-storing tissues,however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as inulinase producers. These hydrolases are usually inducible and their exo-acting forms may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose and raffinose. Fungal inulinase extracts are often produced as stable mixture of highly active fructanhydrolases. From a practical prospective, the best known inulinases to date are those produced by species of Penicillium, Aspergillus and Kluyveromyces. Results: The production of extracellular inulinase by A. kawachii in liquid cultures, using either inulin or yacon derived materials as CES as well as inulinase inducers, is reported. In addition, a partial characterization of the enzyme activity is included. Conclusions: Yacon derived products, particularly yacon juice, added to the culture medium proved to be a good CES for fungal growth as well as an inducer of enzyme synthesis. Partial characterization of the enzyme revealed that it is quite stable in a wide range of pH and temperature. In addition, characterization of the reaction products revealed that this enzyme corresponds to an exo-type. These facts are promising considering its potential application in inulin hydrolysis for the production of high fructose syrups.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3385
Ghiringhelli, Pablo Daniel; Hours, Roque Alberto; Chesini, Mariana; Neila, Lorena Paola; Fratebianchi de la Parra, Dante; et al.; Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate; Pontificia Universidad Catolica de Valparaíso; Electronic Journal Of Biotechnology; 16; 3; 5-2013; 1-13
0717-3458
url http://hdl.handle.net/11336/3385
identifier_str_mv Ghiringhelli, Pablo Daniel; Hours, Roque Alberto; Chesini, Mariana; Neila, Lorena Paola; Fratebianchi de la Parra, Dante; et al.; Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate; Pontificia Universidad Catolica de Valparaíso; Electronic Journal Of Biotechnology; 16; 3; 5-2013; 1-13
0717-3458
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/4h9fbx
info:eu-repo/semantics/altIdentifier/doi/10.2225/vol16-issue3-fulltext-13
info:eu-repo/semantics/altIdentifier/url/www.ejbiotechnology.info/index.php/ejbiotechnology/rt/printerFriendly/v16n3-13/1613
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pontificia Universidad Catolica de Valparaíso
publisher.none.fl_str_mv Pontificia Universidad Catolica de Valparaíso
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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