Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>

Autores
Saparrat, Mario Carlos Nazareno; Guillén, Francisco
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The extracellular ligninolytic enzyme system of Pleurotus laciniatocrenatus, grown under different culture conditions, was characterized and the ability of this strain to degrade different components of Eucalyptus globulus wood was determined. In shaken liquid cultures grown on a C-limited medium supplemented with yeast extract (0.1 %) and peptone (0.5 %), the fungus produced extracellular aryl-alcohol oxidase (Aao), laccase (Lac), manganese-dependent peroxidase (MnP) and manganese-independent peroxidase (MiP) activities, their maximum levels being, respectively, about 600, 50, 1360, and 920 pkat/mL. The supplementation of 1 mmol/L vanillic acid and 150 µmol/L CuSO4 produced an increase of Lac activity levels up to 4-fold and 68.3-fold, respectively. No significant differences were found in the levels of the other ligninolytic enzyme activities when compared to the basal medium. Solid-state fermentation cultures on E. globulus wood chips revealed Lac and MiP activities. These cultures showed degradative activity on lignin and lipophilic wood extractives.
Facultad de Ciencias Naturales y Museo
Instituto de Botánica "Dr. Carlos Spegazzini"
Materia
Ciencias Naturales
Vanillic Acid
Ligninolytic Enzyme
Versatile Peroxidase
Ligninolytic Fungus
Pleurotus Species
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/130851
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/130851
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>Saparrat, Mario Carlos NazarenoGuillén, FranciscoCiencias NaturalesVanillic AcidLigninolytic EnzymeVersatile PeroxidaseLigninolytic FungusPleurotus SpeciesThe extracellular ligninolytic enzyme system of <i>Pleurotus laciniatocrenatus</i>, grown under different culture conditions, was characterized and the ability of this strain to degrade different components of <i>Eucalyptus globulus</i> wood was determined. In shaken liquid cultures grown on a C-limited medium supplemented with yeast extract (0.1 %) and peptone (0.5 %), the fungus produced extracellular aryl-alcohol oxidase (Aao), laccase (Lac), manganese-dependent peroxidase (MnP) and manganese-independent peroxidase (MiP) activities, their maximum levels being, respectively, about 600, 50, 1360, and 920 pkat/mL. The supplementation of 1 mmol/L vanillic acid and 150 µmol/L CuSO4 produced an increase of Lac activity levels up to 4-fold and 68.3-fold, respectively. No significant differences were found in the levels of the other ligninolytic enzyme activities when compared to the basal medium. Solid-state fermentation cultures on <i>E. globulus</i> wood chips revealed Lac and MiP activities. These cultures showed degradative activity on lignin and lipophilic wood extractives.Facultad de Ciencias Naturales y MuseoInstituto de Botánica "Dr. Carlos Spegazzini"2005-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf155-160http://sedici.unlp.edu.ar/handle/10915/130851enginfo:eu-repo/semantics/altIdentifier/issn/0015-5632info:eu-repo/semantics/altIdentifier/issn/1874-9356info:eu-repo/semantics/altIdentifier/issn/0366-662Xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/bf02931465info:eu-repo/semantics/altIdentifier/pmid/16110921info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:37Zoai:sedici.unlp.edu.ar:10915/130851Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:38.097SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>
title Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>
spellingShingle Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>
Saparrat, Mario Carlos Nazareno
Ciencias Naturales
Vanillic Acid
Ligninolytic Enzyme
Versatile Peroxidase
Ligninolytic Fungus
Pleurotus Species
title_short Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>
title_full Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>
title_fullStr Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>
title_full_unstemmed Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>
title_sort Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>
dc.creator.none.fl_str_mv Saparrat, Mario Carlos Nazareno
Guillén, Francisco
author Saparrat, Mario Carlos Nazareno
author_facet Saparrat, Mario Carlos Nazareno
Guillén, Francisco
author_role author
author2 Guillén, Francisco
author2_role author
dc.subject.none.fl_str_mv Ciencias Naturales
Vanillic Acid
Ligninolytic Enzyme
Versatile Peroxidase
Ligninolytic Fungus
Pleurotus Species
topic Ciencias Naturales
Vanillic Acid
Ligninolytic Enzyme
Versatile Peroxidase
Ligninolytic Fungus
Pleurotus Species
dc.description.none.fl_txt_mv The extracellular ligninolytic enzyme system of <i>Pleurotus laciniatocrenatus</i>, grown under different culture conditions, was characterized and the ability of this strain to degrade different components of <i>Eucalyptus globulus</i> wood was determined. In shaken liquid cultures grown on a C-limited medium supplemented with yeast extract (0.1 %) and peptone (0.5 %), the fungus produced extracellular aryl-alcohol oxidase (Aao), laccase (Lac), manganese-dependent peroxidase (MnP) and manganese-independent peroxidase (MiP) activities, their maximum levels being, respectively, about 600, 50, 1360, and 920 pkat/mL. The supplementation of 1 mmol/L vanillic acid and 150 µmol/L CuSO4 produced an increase of Lac activity levels up to 4-fold and 68.3-fold, respectively. No significant differences were found in the levels of the other ligninolytic enzyme activities when compared to the basal medium. Solid-state fermentation cultures on <i>E. globulus</i> wood chips revealed Lac and MiP activities. These cultures showed degradative activity on lignin and lipophilic wood extractives.
Facultad de Ciencias Naturales y Museo
Instituto de Botánica "Dr. Carlos Spegazzini"
description The extracellular ligninolytic enzyme system of <i>Pleurotus laciniatocrenatus</i>, grown under different culture conditions, was characterized and the ability of this strain to degrade different components of <i>Eucalyptus globulus</i> wood was determined. In shaken liquid cultures grown on a C-limited medium supplemented with yeast extract (0.1 %) and peptone (0.5 %), the fungus produced extracellular aryl-alcohol oxidase (Aao), laccase (Lac), manganese-dependent peroxidase (MnP) and manganese-independent peroxidase (MiP) activities, their maximum levels being, respectively, about 600, 50, 1360, and 920 pkat/mL. The supplementation of 1 mmol/L vanillic acid and 150 µmol/L CuSO4 produced an increase of Lac activity levels up to 4-fold and 68.3-fold, respectively. No significant differences were found in the levels of the other ligninolytic enzyme activities when compared to the basal medium. Solid-state fermentation cultures on <i>E. globulus</i> wood chips revealed Lac and MiP activities. These cultures showed degradative activity on lignin and lipophilic wood extractives.
publishDate 2005
dc.date.none.fl_str_mv 2005-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/130851
url http://sedici.unlp.edu.ar/handle/10915/130851
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0015-5632
info:eu-repo/semantics/altIdentifier/issn/1874-9356
info:eu-repo/semantics/altIdentifier/issn/0366-662X
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf02931465
info:eu-repo/semantics/altIdentifier/pmid/16110921
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
155-160
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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