Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine

Autores
Aguilera Sammaritano, Juan Alberto; Caballero, Juan Jose; Deymié Terzi, María Celina; Rosa, Melisa; Vazquez, Fabio; Pappano, Delia Beatriz; Lechner, Bernardo Ernesto; González Teuber, Marcia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Entomopathogenic fungi (EPF) are the natural enemies of insect pests. Nevertheless, research on the use of EPF for simultaneous prevention of pest and disease agents on the same crop is limited. In this study, we explored the potential dual effects of three strains of the EPF Metarhizium anisopliae on the control of detrimental agents of Vitis vinifera L., including different developmental stages (larvae, pupae, and adult) of the insect pest Lobesia botrana and the phytopathogenic fungus Eutypella microtheca. Methods: Laboratory pathogenicity trials were performed to examine the effects of the three M. anisopliae strains on the mortality rate of L. botrana. In addition, field trials were conducted to assess the biocontrol potential of one selected M. anisopliae strain on the larval stage of L. botrana. Moreover, inhibitory effects of the three EPF strains on E. microtheca growth were examined in vitro. Results: All the M. anisopliae strains were highly effective, killing all stages of L. botrana as well as inhibiting the growth of E. microtheca. The in vitro mortality of larvae treated with the strains was over 75%, whereas that of treated pupae and adults was over 85%. The three EPF strains showed similar efficacy against larvae and adult stages; nevertheless, pupal mortality was observed to be strain dependent. Mortality of L. botrana larvae ranged from 64 to 91% at field conditions. Inhibition of E. microtheca growth reached 50% in comparison to the control. Conclusions: Our study showed that M. anisopliae strains were highly effective in ensuring control of two different detrimental agents of V. vinifera L., providing new evidence to support the dual effects of entomopathogenic fungi.
Fil: Aguilera Sammaritano, Juan Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de la Santísima Concepción; Chile
Fil: Caballero, Juan Jose. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Deymié Terzi, María Celina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rosa, Melisa. Universidad Nacional de San Juan; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Pappano, Delia Beatriz. Universidad Nacional de San Juan; Argentina
Fil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: González Teuber, Marcia. Universidad Católica de la Santísima Concepción; Chile
Materia
ENTOMOPATHOGENIC FUNGI
EUROPEAN GRAPEVINE MOTH
EUTYPELLA MICROTHECA
GRAPEVINE
LIGNINOLYTIC FUNGUS
LOBESIA BOTRANA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/153379
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevineAguilera Sammaritano, Juan AlbertoCaballero, Juan JoseDeymié Terzi, María CelinaRosa, MelisaVazquez, FabioPappano, Delia BeatrizLechner, Bernardo ErnestoGonzález Teuber, MarciaENTOMOPATHOGENIC FUNGIEUROPEAN GRAPEVINE MOTHEUTYPELLA MICROTHECAGRAPEVINELIGNINOLYTIC FUNGUSLOBESIA BOTRANAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Entomopathogenic fungi (EPF) are the natural enemies of insect pests. Nevertheless, research on the use of EPF for simultaneous prevention of pest and disease agents on the same crop is limited. In this study, we explored the potential dual effects of three strains of the EPF Metarhizium anisopliae on the control of detrimental agents of Vitis vinifera L., including different developmental stages (larvae, pupae, and adult) of the insect pest Lobesia botrana and the phytopathogenic fungus Eutypella microtheca. Methods: Laboratory pathogenicity trials were performed to examine the effects of the three M. anisopliae strains on the mortality rate of L. botrana. In addition, field trials were conducted to assess the biocontrol potential of one selected M. anisopliae strain on the larval stage of L. botrana. Moreover, inhibitory effects of the three EPF strains on E. microtheca growth were examined in vitro. Results: All the M. anisopliae strains were highly effective, killing all stages of L. botrana as well as inhibiting the growth of E. microtheca. The in vitro mortality of larvae treated with the strains was over 75%, whereas that of treated pupae and adults was over 85%. The three EPF strains showed similar efficacy against larvae and adult stages; nevertheless, pupal mortality was observed to be strain dependent. Mortality of L. botrana larvae ranged from 64 to 91% at field conditions. Inhibition of E. microtheca growth reached 50% in comparison to the control. Conclusions: Our study showed that M. anisopliae strains were highly effective in ensuring control of two different detrimental agents of V. vinifera L., providing new evidence to support the dual effects of entomopathogenic fungi.Fil: Aguilera Sammaritano, Juan Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de la Santísima Concepción; ChileFil: Caballero, Juan Jose. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Deymié Terzi, María Celina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rosa, Melisa. Universidad Nacional de San Juan; ArgentinaFil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Pappano, Delia Beatriz. Universidad Nacional de San Juan; ArgentinaFil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: González Teuber, Marcia. Universidad Católica de la Santísima Concepción; ChileSociedad de Biología de Chile2021-12-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153379Aguilera Sammaritano, Juan Alberto; Caballero, Juan Jose; Deymié Terzi, María Celina; Rosa, Melisa; Vazquez, Fabio; et al.; Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine; Sociedad de Biología de Chile; Biological Research; 54; 1; 13-12-2021; 1-100716-97600717-6287CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biolres.biomedcentral.com/articles/10.1186/s40659-021-00367-xinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s40659-021-00367-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:40Zoai:ri.conicet.gov.ar:11336/153379instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:40.649CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine
title Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine
spellingShingle Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine
Aguilera Sammaritano, Juan Alberto
ENTOMOPATHOGENIC FUNGI
EUROPEAN GRAPEVINE MOTH
EUTYPELLA MICROTHECA
GRAPEVINE
LIGNINOLYTIC FUNGUS
LOBESIA BOTRANA
title_short Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine
title_full Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine
title_fullStr Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine
title_full_unstemmed Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine
title_sort Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine
dc.creator.none.fl_str_mv Aguilera Sammaritano, Juan Alberto
Caballero, Juan Jose
Deymié Terzi, María Celina
Rosa, Melisa
Vazquez, Fabio
Pappano, Delia Beatriz
Lechner, Bernardo Ernesto
González Teuber, Marcia
author Aguilera Sammaritano, Juan Alberto
author_facet Aguilera Sammaritano, Juan Alberto
Caballero, Juan Jose
Deymié Terzi, María Celina
Rosa, Melisa
Vazquez, Fabio
Pappano, Delia Beatriz
Lechner, Bernardo Ernesto
González Teuber, Marcia
author_role author
author2 Caballero, Juan Jose
Deymié Terzi, María Celina
Rosa, Melisa
Vazquez, Fabio
Pappano, Delia Beatriz
Lechner, Bernardo Ernesto
González Teuber, Marcia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ENTOMOPATHOGENIC FUNGI
EUROPEAN GRAPEVINE MOTH
EUTYPELLA MICROTHECA
GRAPEVINE
LIGNINOLYTIC FUNGUS
LOBESIA BOTRANA
topic ENTOMOPATHOGENIC FUNGI
EUROPEAN GRAPEVINE MOTH
EUTYPELLA MICROTHECA
GRAPEVINE
LIGNINOLYTIC FUNGUS
LOBESIA BOTRANA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Entomopathogenic fungi (EPF) are the natural enemies of insect pests. Nevertheless, research on the use of EPF for simultaneous prevention of pest and disease agents on the same crop is limited. In this study, we explored the potential dual effects of three strains of the EPF Metarhizium anisopliae on the control of detrimental agents of Vitis vinifera L., including different developmental stages (larvae, pupae, and adult) of the insect pest Lobesia botrana and the phytopathogenic fungus Eutypella microtheca. Methods: Laboratory pathogenicity trials were performed to examine the effects of the three M. anisopliae strains on the mortality rate of L. botrana. In addition, field trials were conducted to assess the biocontrol potential of one selected M. anisopliae strain on the larval stage of L. botrana. Moreover, inhibitory effects of the three EPF strains on E. microtheca growth were examined in vitro. Results: All the M. anisopliae strains were highly effective, killing all stages of L. botrana as well as inhibiting the growth of E. microtheca. The in vitro mortality of larvae treated with the strains was over 75%, whereas that of treated pupae and adults was over 85%. The three EPF strains showed similar efficacy against larvae and adult stages; nevertheless, pupal mortality was observed to be strain dependent. Mortality of L. botrana larvae ranged from 64 to 91% at field conditions. Inhibition of E. microtheca growth reached 50% in comparison to the control. Conclusions: Our study showed that M. anisopliae strains were highly effective in ensuring control of two different detrimental agents of V. vinifera L., providing new evidence to support the dual effects of entomopathogenic fungi.
Fil: Aguilera Sammaritano, Juan Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de la Santísima Concepción; Chile
Fil: Caballero, Juan Jose. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Deymié Terzi, María Celina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rosa, Melisa. Universidad Nacional de San Juan; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Pappano, Delia Beatriz. Universidad Nacional de San Juan; Argentina
Fil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: González Teuber, Marcia. Universidad Católica de la Santísima Concepción; Chile
description Background: Entomopathogenic fungi (EPF) are the natural enemies of insect pests. Nevertheless, research on the use of EPF for simultaneous prevention of pest and disease agents on the same crop is limited. In this study, we explored the potential dual effects of three strains of the EPF Metarhizium anisopliae on the control of detrimental agents of Vitis vinifera L., including different developmental stages (larvae, pupae, and adult) of the insect pest Lobesia botrana and the phytopathogenic fungus Eutypella microtheca. Methods: Laboratory pathogenicity trials were performed to examine the effects of the three M. anisopliae strains on the mortality rate of L. botrana. In addition, field trials were conducted to assess the biocontrol potential of one selected M. anisopliae strain on the larval stage of L. botrana. Moreover, inhibitory effects of the three EPF strains on E. microtheca growth were examined in vitro. Results: All the M. anisopliae strains were highly effective, killing all stages of L. botrana as well as inhibiting the growth of E. microtheca. The in vitro mortality of larvae treated with the strains was over 75%, whereas that of treated pupae and adults was over 85%. The three EPF strains showed similar efficacy against larvae and adult stages; nevertheless, pupal mortality was observed to be strain dependent. Mortality of L. botrana larvae ranged from 64 to 91% at field conditions. Inhibition of E. microtheca growth reached 50% in comparison to the control. Conclusions: Our study showed that M. anisopliae strains were highly effective in ensuring control of two different detrimental agents of V. vinifera L., providing new evidence to support the dual effects of entomopathogenic fungi.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/153379
Aguilera Sammaritano, Juan Alberto; Caballero, Juan Jose; Deymié Terzi, María Celina; Rosa, Melisa; Vazquez, Fabio; et al.; Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine; Sociedad de Biología de Chile; Biological Research; 54; 1; 13-12-2021; 1-10
0716-9760
0717-6287
CONICET Digital
CONICET
url http://hdl.handle.net/11336/153379
identifier_str_mv Aguilera Sammaritano, Juan Alberto; Caballero, Juan Jose; Deymié Terzi, María Celina; Rosa, Melisa; Vazquez, Fabio; et al.; Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine; Sociedad de Biología de Chile; Biological Research; 54; 1; 13-12-2021; 1-10
0716-9760
0717-6287
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biolres.biomedcentral.com/articles/10.1186/s40659-021-00367-x
info:eu-repo/semantics/altIdentifier/doi/10.1186/s40659-021-00367-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad de Biología de Chile
publisher.none.fl_str_mv Sociedad de Biología de Chile
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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