Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme production

Autores
Grassi, Emanuel Marcelo; Pildain, Maria Belen; Levin, Laura Noemi; Carmaran, Cecilia Cristina
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study the extracellular production of ligninolytic enzymes by xylariaceous and diatrypaceous species from Argentina was investigated. Among them, a new species (Eutypa microasca) was evaluated. Its morphological characteristics (e.g.: very small asci), along with the molecular analyses, support the hypothesis of the new taxon. Ligninolytic enzyme production by several saprophytic and endophytic strains belonging to the genera Xylaria, Eutypella, Eutypa and Peroneutypa was assessed on solid medium supplemented with different dyes (Poly R-478, Azure B and Malachite Green) and under submerged fermentation in copper amended malt extract/glucose medium. All the strains produced laccase in liquid cultures, and seven out of nine also produced Mn-peroxidase (highest titres detected 113 U/l). Only 5 of the strains assayed could not decolorize Malachite Green. This is the first report on laccase and Mn-peroxidase production by the members of the Diatrypaceae and contributes to the knowledge on ligninolytic enzyme production in the members of the Xylariaceae, scarcely studied up to date.
Fil: Grassi, Emanuel Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
Fil: Pildain, Maria Belen. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina
Fil: Levin, Laura Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
Fil: Carmaran, Cecilia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
Materia
Diatrypaceae
Eutypa Microasca
Ligninolytic Enzymes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17811
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme productionGrassi, Emanuel MarceloPildain, Maria BelenLevin, Laura NoemiCarmaran, Cecilia CristinaDiatrypaceaeEutypa MicroascaLigninolytic Enzymeshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this study the extracellular production of ligninolytic enzymes by xylariaceous and diatrypaceous species from Argentina was investigated. Among them, a new species (Eutypa microasca) was evaluated. Its morphological characteristics (e.g.: very small asci), along with the molecular analyses, support the hypothesis of the new taxon. Ligninolytic enzyme production by several saprophytic and endophytic strains belonging to the genera Xylaria, Eutypella, Eutypa and Peroneutypa was assessed on solid medium supplemented with different dyes (Poly R-478, Azure B and Malachite Green) and under submerged fermentation in copper amended malt extract/glucose medium. All the strains produced laccase in liquid cultures, and seven out of nine also produced Mn-peroxidase (highest titres detected 113 U/l). Only 5 of the strains assayed could not decolorize Malachite Green. This is the first report on laccase and Mn-peroxidase production by the members of the Diatrypaceae and contributes to the knowledge on ligninolytic enzyme production in the members of the Xylariaceae, scarcely studied up to date.Fil: Grassi, Emanuel Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; ArgentinaFil: Pildain, Maria Belen. Centro de Investigación y Extensión Forestal Andino Patagónico; ArgentinaFil: Levin, Laura Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; ArgentinaFil: Carmaran, Cecilia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; ArgentinaVerlag Ferdinand Berger Sohne Gesellschaft Mbh2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17811Grassi, Emanuel Marcelo; Pildain, Maria Belen; Levin, Laura Noemi; Carmaran, Cecilia Cristina; Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme production; Verlag Ferdinand Berger Sohne Gesellschaft Mbh; Sydowia; 66; 1; 6-2014; 99-1140082-0598enginfo:eu-repo/semantics/altIdentifier/url/http://www.sydowia.at/syd66-1/T9-Grasi.htmlinfo:eu-repo/semantics/altIdentifier/doi/10.12905/0380.sydowia66(1)2014-0099info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:02Zoai:ri.conicet.gov.ar:11336/17811instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:02.475CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme production
title Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme production
spellingShingle Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme production
Grassi, Emanuel Marcelo
Diatrypaceae
Eutypa Microasca
Ligninolytic Enzymes
title_short Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme production
title_full Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme production
title_fullStr Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme production
title_full_unstemmed Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme production
title_sort Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme production
dc.creator.none.fl_str_mv Grassi, Emanuel Marcelo
Pildain, Maria Belen
Levin, Laura Noemi
Carmaran, Cecilia Cristina
author Grassi, Emanuel Marcelo
author_facet Grassi, Emanuel Marcelo
Pildain, Maria Belen
Levin, Laura Noemi
Carmaran, Cecilia Cristina
author_role author
author2 Pildain, Maria Belen
Levin, Laura Noemi
Carmaran, Cecilia Cristina
author2_role author
author
author
dc.subject.none.fl_str_mv Diatrypaceae
Eutypa Microasca
Ligninolytic Enzymes
topic Diatrypaceae
Eutypa Microasca
Ligninolytic Enzymes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this study the extracellular production of ligninolytic enzymes by xylariaceous and diatrypaceous species from Argentina was investigated. Among them, a new species (Eutypa microasca) was evaluated. Its morphological characteristics (e.g.: very small asci), along with the molecular analyses, support the hypothesis of the new taxon. Ligninolytic enzyme production by several saprophytic and endophytic strains belonging to the genera Xylaria, Eutypella, Eutypa and Peroneutypa was assessed on solid medium supplemented with different dyes (Poly R-478, Azure B and Malachite Green) and under submerged fermentation in copper amended malt extract/glucose medium. All the strains produced laccase in liquid cultures, and seven out of nine also produced Mn-peroxidase (highest titres detected 113 U/l). Only 5 of the strains assayed could not decolorize Malachite Green. This is the first report on laccase and Mn-peroxidase production by the members of the Diatrypaceae and contributes to the knowledge on ligninolytic enzyme production in the members of the Xylariaceae, scarcely studied up to date.
Fil: Grassi, Emanuel Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
Fil: Pildain, Maria Belen. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina
Fil: Levin, Laura Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
Fil: Carmaran, Cecilia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
description In this study the extracellular production of ligninolytic enzymes by xylariaceous and diatrypaceous species from Argentina was investigated. Among them, a new species (Eutypa microasca) was evaluated. Its morphological characteristics (e.g.: very small asci), along with the molecular analyses, support the hypothesis of the new taxon. Ligninolytic enzyme production by several saprophytic and endophytic strains belonging to the genera Xylaria, Eutypella, Eutypa and Peroneutypa was assessed on solid medium supplemented with different dyes (Poly R-478, Azure B and Malachite Green) and under submerged fermentation in copper amended malt extract/glucose medium. All the strains produced laccase in liquid cultures, and seven out of nine also produced Mn-peroxidase (highest titres detected 113 U/l). Only 5 of the strains assayed could not decolorize Malachite Green. This is the first report on laccase and Mn-peroxidase production by the members of the Diatrypaceae and contributes to the knowledge on ligninolytic enzyme production in the members of the Xylariaceae, scarcely studied up to date.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17811
Grassi, Emanuel Marcelo; Pildain, Maria Belen; Levin, Laura Noemi; Carmaran, Cecilia Cristina; Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme production; Verlag Ferdinand Berger Sohne Gesellschaft Mbh; Sydowia; 66; 1; 6-2014; 99-114
0082-0598
url http://hdl.handle.net/11336/17811
identifier_str_mv Grassi, Emanuel Marcelo; Pildain, Maria Belen; Levin, Laura Noemi; Carmaran, Cecilia Cristina; Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme production; Verlag Ferdinand Berger Sohne Gesellschaft Mbh; Sydowia; 66; 1; 6-2014; 99-114
0082-0598
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sydowia.at/syd66-1/T9-Grasi.html
info:eu-repo/semantics/altIdentifier/doi/10.12905/0380.sydowia66(1)2014-0099
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Verlag Ferdinand Berger Sohne Gesellschaft Mbh
publisher.none.fl_str_mv Verlag Ferdinand Berger Sohne Gesellschaft Mbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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