Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH

Autores
Caetano Anollés, Gustavo; Lagares, Antonio; Favelukes, Gabriel
Año de publicación
1989
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adsorption of Rhizobium meliloti L5-30 in low numbers to alfalfa (Medicago sativa L.) roots was dependent on the presence of divalent cations, and required neutral pH. Adsorption was proportional to Ca and/or Mg concentrations up to 1.5 mM. Ca was not substituted by Sr, Ba or Mn. Adsorption was abolished and viability decreased at pH≤6. When lowering pH, higher Ca concentrations were required to attain similar adsorption levels, indicating a marked interactive effect between Ca and H ions. Pretreatment of the roots with Ca and low pH did not affect subsequent adsorption of the bacteria. However, Ca pretreatment ofR. meliloti sustained further adsorption at low Ca levels and low pH substantially affected their ability to adsorb. Low pH appears to affect the stability of binding causing desorption of the previously bound bacteria. The presence of saturating concentrations of heterologousR. leguminosarum bv.trifolii A118, did not prevent the expression of divalent cations and pH requirements, as well as their interaction. Our results suggest that rhizobial binding to the root surface already shows the Ca and pH dependence of alfalfa nodulation, which was generally associated to some event prior to rhizobial penetration of root hairs.
Facultad de Ciencias Exactas
Materia
Química
Bacterial adsorption
Calcium
Magnesium
Medicago sativa
pH
Rhizobium meliloti
Root surface
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/136321
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/136321
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pHCaetano Anollés, GustavoLagares, AntonioFavelukes, GabrielQuímicaBacterial adsorptionCalciumMagnesiumMedicago sativapHRhizobium melilotiRoot surfaceAdsorption of Rhizobium meliloti L5-30 in low numbers to alfalfa (Medicago sativa L.) roots was dependent on the presence of divalent cations, and required neutral pH. Adsorption was proportional to Ca and/or Mg concentrations up to 1.5 mM. Ca was not substituted by Sr, Ba or Mn. Adsorption was abolished and viability decreased at pH≤6. When lowering pH, higher Ca concentrations were required to attain similar adsorption levels, indicating a marked interactive effect between Ca and H ions. Pretreatment of the roots with Ca and low pH did not affect subsequent adsorption of the bacteria. However, Ca pretreatment ofR. meliloti sustained further adsorption at low Ca levels and low pH substantially affected their ability to adsorb. Low pH appears to affect the stability of binding causing desorption of the previously bound bacteria. The presence of saturating concentrations of heterologousR. leguminosarum bv.trifolii A118, did not prevent the expression of divalent cations and pH requirements, as well as their interaction. Our results suggest that rhizobial binding to the root surface already shows the Ca and pH dependence of alfalfa nodulation, which was generally associated to some event prior to rhizobial penetration of root hairs.Facultad de Ciencias Exactas1989info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf67-74http://sedici.unlp.edu.ar/handle/10915/136321enginfo:eu-repo/semantics/altIdentifier/issn/0032-079Xinfo:eu-repo/semantics/altIdentifier/issn/1573-5036info:eu-repo/semantics/altIdentifier/doi/10.1007/bf02206258info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:21Zoai:sedici.unlp.edu.ar:10915/136321Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:22.229SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH
title Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH
spellingShingle Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH
Caetano Anollés, Gustavo
Química
Bacterial adsorption
Calcium
Magnesium
Medicago sativa
pH
Rhizobium meliloti
Root surface
title_short Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH
title_full Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH
title_fullStr Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH
title_full_unstemmed Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH
title_sort Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH
dc.creator.none.fl_str_mv Caetano Anollés, Gustavo
Lagares, Antonio
Favelukes, Gabriel
author Caetano Anollés, Gustavo
author_facet Caetano Anollés, Gustavo
Lagares, Antonio
Favelukes, Gabriel
author_role author
author2 Lagares, Antonio
Favelukes, Gabriel
author2_role author
author
dc.subject.none.fl_str_mv Química
Bacterial adsorption
Calcium
Magnesium
Medicago sativa
pH
Rhizobium meliloti
Root surface
topic Química
Bacterial adsorption
Calcium
Magnesium
Medicago sativa
pH
Rhizobium meliloti
Root surface
dc.description.none.fl_txt_mv Adsorption of Rhizobium meliloti L5-30 in low numbers to alfalfa (Medicago sativa L.) roots was dependent on the presence of divalent cations, and required neutral pH. Adsorption was proportional to Ca and/or Mg concentrations up to 1.5 mM. Ca was not substituted by Sr, Ba or Mn. Adsorption was abolished and viability decreased at pH≤6. When lowering pH, higher Ca concentrations were required to attain similar adsorption levels, indicating a marked interactive effect between Ca and H ions. Pretreatment of the roots with Ca and low pH did not affect subsequent adsorption of the bacteria. However, Ca pretreatment ofR. meliloti sustained further adsorption at low Ca levels and low pH substantially affected their ability to adsorb. Low pH appears to affect the stability of binding causing desorption of the previously bound bacteria. The presence of saturating concentrations of heterologousR. leguminosarum bv.trifolii A118, did not prevent the expression of divalent cations and pH requirements, as well as their interaction. Our results suggest that rhizobial binding to the root surface already shows the Ca and pH dependence of alfalfa nodulation, which was generally associated to some event prior to rhizobial penetration of root hairs.
Facultad de Ciencias Exactas
description Adsorption of Rhizobium meliloti L5-30 in low numbers to alfalfa (Medicago sativa L.) roots was dependent on the presence of divalent cations, and required neutral pH. Adsorption was proportional to Ca and/or Mg concentrations up to 1.5 mM. Ca was not substituted by Sr, Ba or Mn. Adsorption was abolished and viability decreased at pH≤6. When lowering pH, higher Ca concentrations were required to attain similar adsorption levels, indicating a marked interactive effect between Ca and H ions. Pretreatment of the roots with Ca and low pH did not affect subsequent adsorption of the bacteria. However, Ca pretreatment ofR. meliloti sustained further adsorption at low Ca levels and low pH substantially affected their ability to adsorb. Low pH appears to affect the stability of binding causing desorption of the previously bound bacteria. The presence of saturating concentrations of heterologousR. leguminosarum bv.trifolii A118, did not prevent the expression of divalent cations and pH requirements, as well as their interaction. Our results suggest that rhizobial binding to the root surface already shows the Ca and pH dependence of alfalfa nodulation, which was generally associated to some event prior to rhizobial penetration of root hairs.
publishDate 1989
dc.date.none.fl_str_mv 1989
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/136321
url http://sedici.unlp.edu.ar/handle/10915/136321
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0032-079X
info:eu-repo/semantics/altIdentifier/issn/1573-5036
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf02206258
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
67-74
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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