Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production
- Autores
- Ortega, Leonel Maximiliano; Alconada Magliano, Teresa María; Astoreca, Andrea Luciana; Kikot, Gisele Eleonora
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fusarium graminearum, the main etiological agent of Fusarium Head Bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present report, several F. graminearum isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic and lipase extracellular enzymatic activities production, as well as by the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation with the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research, would allow us to apply a selection criterion among isolates for further research.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales - Materia
-
Ciencias Exactas
Biología
Química
enzymes extracellular
deoxynivalenol
Fusarium graminearum isolates
screening - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/3.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/104505
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Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol ProductionOrtega, Leonel MaximilianoAlconada Magliano, Teresa MaríaAstoreca, Andrea LucianaKikot, Gisele EleonoraCiencias ExactasBiologíaQuímicaenzymes extracellulardeoxynivalenolFusarium graminearum isolatesscreening<i>Fusarium graminearum</i>, the main etiological agent of Fusarium Head Bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present report, several <i>F. graminearum</i> isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic and lipase extracellular enzymatic activities production, as well as by the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation with the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research, would allow us to apply a selection criterion among isolates for further research.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriales2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/104505enginfo:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/4307info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/jmy/2013/358140/info:eu-repo/semantics/altIdentifier/issn/2314-6168info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/358140info:eu-repo/semantics/altIdentifier/hdl/11336/4307info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/Creative Commons Attribution 3.0 Unported (CC BY 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:54:55Zoai:sedici.unlp.edu.ar:10915/104505Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:54:56.199SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production |
title |
Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production |
spellingShingle |
Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production Ortega, Leonel Maximiliano Ciencias Exactas Biología Química enzymes extracellular deoxynivalenol Fusarium graminearum isolates screening |
title_short |
Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production |
title_full |
Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production |
title_fullStr |
Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production |
title_full_unstemmed |
Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production |
title_sort |
Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production |
dc.creator.none.fl_str_mv |
Ortega, Leonel Maximiliano Alconada Magliano, Teresa María Astoreca, Andrea Luciana Kikot, Gisele Eleonora |
author |
Ortega, Leonel Maximiliano |
author_facet |
Ortega, Leonel Maximiliano Alconada Magliano, Teresa María Astoreca, Andrea Luciana Kikot, Gisele Eleonora |
author_role |
author |
author2 |
Alconada Magliano, Teresa María Astoreca, Andrea Luciana Kikot, Gisele Eleonora |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Biología Química enzymes extracellular deoxynivalenol Fusarium graminearum isolates screening |
topic |
Ciencias Exactas Biología Química enzymes extracellular deoxynivalenol Fusarium graminearum isolates screening |
dc.description.none.fl_txt_mv |
<i>Fusarium graminearum</i>, the main etiological agent of Fusarium Head Bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present report, several <i>F. graminearum</i> isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic and lipase extracellular enzymatic activities production, as well as by the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation with the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research, would allow us to apply a selection criterion among isolates for further research. Facultad de Ciencias Exactas Centro de Investigación y Desarrollo en Fermentaciones Industriales |
description |
<i>Fusarium graminearum</i>, the main etiological agent of Fusarium Head Bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present report, several <i>F. graminearum</i> isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic and lipase extracellular enzymatic activities production, as well as by the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation with the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research, would allow us to apply a selection criterion among isolates for further research. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/104505 |
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http://sedici.unlp.edu.ar/handle/10915/104505 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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openAccess |
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http://creativecommons.org/licenses/by/3.0/ Creative Commons Attribution 3.0 Unported (CC BY 3.0) |
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