Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production

Autores
Ortega, Leonel Maximiliano; Alconada Magliano, Teresa María; Astoreca, Andrea Luciana; Kikot, Gisele Eleonora
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fusarium graminearum, the main etiological agent of Fusarium Head Bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present report, several F. graminearum isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic and lipase extracellular enzymatic activities production, as well as by the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation with the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research, would allow us to apply a selection criterion among isolates for further research.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
Biología
Química
enzymes extracellular
deoxynivalenol
Fusarium graminearum isolates
screening
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/3.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/104505

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spelling Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol ProductionOrtega, Leonel MaximilianoAlconada Magliano, Teresa MaríaAstoreca, Andrea LucianaKikot, Gisele EleonoraCiencias ExactasBiologíaQuímicaenzymes extracellulardeoxynivalenolFusarium graminearum isolatesscreening<i>Fusarium graminearum</i>, the main etiological agent of Fusarium Head Bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present report, several <i>F. graminearum</i> isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic and lipase extracellular enzymatic activities production, as well as by the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation with the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research, would allow us to apply a selection criterion among isolates for further research.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriales2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/104505enginfo:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/4307info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/jmy/2013/358140/info:eu-repo/semantics/altIdentifier/issn/2314-6168info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/358140info:eu-repo/semantics/altIdentifier/hdl/11336/4307info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/Creative Commons Attribution 3.0 Unported (CC BY 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:54:55Zoai:sedici.unlp.edu.ar:10915/104505Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:54:56.199SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production
title Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production
spellingShingle Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production
Ortega, Leonel Maximiliano
Ciencias Exactas
Biología
Química
enzymes extracellular
deoxynivalenol
Fusarium graminearum isolates
screening
title_short Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production
title_full Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production
title_fullStr Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production
title_full_unstemmed Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production
title_sort Screening of <i>Fusarium graminearum</i> Isolates for Enzymes Extracellular and Deoxynivalenol Production
dc.creator.none.fl_str_mv Ortega, Leonel Maximiliano
Alconada Magliano, Teresa María
Astoreca, Andrea Luciana
Kikot, Gisele Eleonora
author Ortega, Leonel Maximiliano
author_facet Ortega, Leonel Maximiliano
Alconada Magliano, Teresa María
Astoreca, Andrea Luciana
Kikot, Gisele Eleonora
author_role author
author2 Alconada Magliano, Teresa María
Astoreca, Andrea Luciana
Kikot, Gisele Eleonora
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
Química
enzymes extracellular
deoxynivalenol
Fusarium graminearum isolates
screening
topic Ciencias Exactas
Biología
Química
enzymes extracellular
deoxynivalenol
Fusarium graminearum isolates
screening
dc.description.none.fl_txt_mv <i>Fusarium graminearum</i>, the main etiological agent of Fusarium Head Bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present report, several <i>F. graminearum</i> isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic and lipase extracellular enzymatic activities production, as well as by the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation with the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research, would allow us to apply a selection criterion among isolates for further research.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description <i>Fusarium graminearum</i>, the main etiological agent of Fusarium Head Bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present report, several <i>F. graminearum</i> isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic and lipase extracellular enzymatic activities production, as well as by the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation with the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research, would allow us to apply a selection criterion among isolates for further research.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/104505
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dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/jmy/2013/358140/
info:eu-repo/semantics/altIdentifier/issn/2314-6168
info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/358140
info:eu-repo/semantics/altIdentifier/hdl/11336/4307
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/
Creative Commons Attribution 3.0 Unported (CC BY 3.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/
Creative Commons Attribution 3.0 Unported (CC BY 3.0)
dc.format.none.fl_str_mv application/pdf
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institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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