Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina

Autores
Ortega, Leonel Maximiliano; Dinolfo, María Inés; Astoreca, Andrea Luciana; Alberione, Enrique Javier; Stenglein, Sebastián Alberto; Alconada Magliano, Teresa María
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects yield losses and quality of grains, reducing the wheat end-use, also causing mycotoxin contamination. In this study, the genetic variability and deoxynivalenol (DON) potential/production of F. graminearum sensu stricto (s.s.) isolates obtained from wheat samples of the 2009, 2010, and 2011 growing seasons from a single location in Argentina were evaluated. The genetic variability detected using inter-simple sequence repeat (ISSR) was analyzed in relation to the in vitro deoxynivalenol production, the main monitored and quantified mycotoxin according to the current regulations for the international marketing of cereals. Of the 68 F. graminearum s.s. isolates obtained in this study, 95 % showed a different banding pattern with ISSR molecular markers and a high variability was detected within the population. However, no clustering was found in relation with year or DON production. All isolates amplify for the DON-related gene and a high variability in DON production was observed among the isolates, with production values between non-producers and 1741 μg/g. The results suggest that the F. graminearum s.s. population varies significantly in both genetic structure and toxin production in a limited sampled area.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Química
Fusarium graminearum
Wheat
Genetic variability
Deoxynivalenol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/133323
Acceder
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network_name_str SEDICI (UNLP)
spelling Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in ArgentinaOrtega, Leonel MaximilianoDinolfo, María InésAstoreca, Andrea LucianaAlberione, Enrique JavierStenglein, Sebastián AlbertoAlconada Magliano, Teresa MaríaQuímicaFusarium graminearumWheatGenetic variabilityDeoxynivalenolFusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects yield losses and quality of grains, reducing the wheat end-use, also causing mycotoxin contamination. In this study, the genetic variability and deoxynivalenol (DON) potential/production of F. graminearum sensu stricto (s.s.) isolates obtained from wheat samples of the 2009, 2010, and 2011 growing seasons from a single location in Argentina were evaluated. The genetic variability detected using inter-simple sequence repeat (ISSR) was analyzed in relation to the in vitro deoxynivalenol production, the main monitored and quantified mycotoxin according to the current regulations for the international marketing of cereals. Of the 68 F. graminearum s.s. isolates obtained in this study, 95 % showed a different banding pattern with ISSR molecular markers and a high variability was detected within the population. However, no clustering was found in relation with year or DON production. All isolates amplify for the DON-related gene and a high variability in DON production was observed among the isolates, with production values between non-producers and 1741 μg/g. The results suggest that the F. graminearum s.s. population varies significantly in both genetic structure and toxin production in a limited sampled area.Centro de Investigación y Desarrollo en Fermentaciones Industriales2015-12-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-8http://sedici.unlp.edu.ar/handle/10915/133323enginfo:eu-repo/semantics/altIdentifier/issn/1617-416Xinfo:eu-repo/semantics/altIdentifier/issn/1861-8952info:eu-repo/semantics/altIdentifier/doi/10.1007/s11557-015-1147-7info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:03:49Zoai:sedici.unlp.edu.ar:10915/133323Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:03:49.447SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
title Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
spellingShingle Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
Ortega, Leonel Maximiliano
Química
Fusarium graminearum
Wheat
Genetic variability
Deoxynivalenol
title_short Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
title_full Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
title_fullStr Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
title_full_unstemmed Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
title_sort Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
dc.creator.none.fl_str_mv Ortega, Leonel Maximiliano
Dinolfo, María Inés
Astoreca, Andrea Luciana
Alberione, Enrique Javier
Stenglein, Sebastián Alberto
Alconada Magliano, Teresa María
author Ortega, Leonel Maximiliano
author_facet Ortega, Leonel Maximiliano
Dinolfo, María Inés
Astoreca, Andrea Luciana
Alberione, Enrique Javier
Stenglein, Sebastián Alberto
Alconada Magliano, Teresa María
author_role author
author2 Dinolfo, María Inés
Astoreca, Andrea Luciana
Alberione, Enrique Javier
Stenglein, Sebastián Alberto
Alconada Magliano, Teresa María
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Química
Fusarium graminearum
Wheat
Genetic variability
Deoxynivalenol
topic Química
Fusarium graminearum
Wheat
Genetic variability
Deoxynivalenol
dc.description.none.fl_txt_mv Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects yield losses and quality of grains, reducing the wheat end-use, also causing mycotoxin contamination. In this study, the genetic variability and deoxynivalenol (DON) potential/production of F. graminearum sensu stricto (s.s.) isolates obtained from wheat samples of the 2009, 2010, and 2011 growing seasons from a single location in Argentina were evaluated. The genetic variability detected using inter-simple sequence repeat (ISSR) was analyzed in relation to the in vitro deoxynivalenol production, the main monitored and quantified mycotoxin according to the current regulations for the international marketing of cereals. Of the 68 F. graminearum s.s. isolates obtained in this study, 95 % showed a different banding pattern with ISSR molecular markers and a high variability was detected within the population. However, no clustering was found in relation with year or DON production. All isolates amplify for the DON-related gene and a high variability in DON production was observed among the isolates, with production values between non-producers and 1741 μg/g. The results suggest that the F. graminearum s.s. population varies significantly in both genetic structure and toxin production in a limited sampled area.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects yield losses and quality of grains, reducing the wheat end-use, also causing mycotoxin contamination. In this study, the genetic variability and deoxynivalenol (DON) potential/production of F. graminearum sensu stricto (s.s.) isolates obtained from wheat samples of the 2009, 2010, and 2011 growing seasons from a single location in Argentina were evaluated. The genetic variability detected using inter-simple sequence repeat (ISSR) was analyzed in relation to the in vitro deoxynivalenol production, the main monitored and quantified mycotoxin according to the current regulations for the international marketing of cereals. Of the 68 F. graminearum s.s. isolates obtained in this study, 95 % showed a different banding pattern with ISSR molecular markers and a high variability was detected within the population. However, no clustering was found in relation with year or DON production. All isolates amplify for the DON-related gene and a high variability in DON production was observed among the isolates, with production values between non-producers and 1741 μg/g. The results suggest that the F. graminearum s.s. population varies significantly in both genetic structure and toxin production in a limited sampled area.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/133323
url http://sedici.unlp.edu.ar/handle/10915/133323
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1617-416X
info:eu-repo/semantics/altIdentifier/issn/1861-8952
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11557-015-1147-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
1-8
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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