Screening of Fusarium graminearum isolates for enzymes extracellular and deoxynivalenol production

Autores
Ortega, Leonel Maximilano; Alconada Magliano, Teresa Maria; Astoreca, Andrea Luciana; Kikot, Gisele Eleonora
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fusarium graminearum, the main etiological agent of Fusarium Head Bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present report, several F. graminearum isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic and lipase extracellular enzymatic activities production, as well as by the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation with the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research, would allow us to apply a selection criterion among isolates for further research.
Fil: Ortega, Leonel Maximilano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Alconada Magliano, Teresa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Astoreca, Andrea Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Kikot, Gisele Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Materia
ENZYMES EXTRACELLULAR
DEOXYNIVALENOL
FUSARIUM GRAMINEARUM ISOLATES
SCREENING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4307
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Screening of Fusarium graminearum isolates for enzymes extracellular and deoxynivalenol productionOrtega, Leonel MaximilanoAlconada Magliano, Teresa MariaAstoreca, Andrea LucianaKikot, Gisele EleonoraENZYMES EXTRACELLULARDEOXYNIVALENOLFUSARIUM GRAMINEARUM ISOLATESSCREENINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fusarium graminearum, the main etiological agent of Fusarium Head Bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present report, several F. graminearum isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic and lipase extracellular enzymatic activities production, as well as by the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation with the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research, would allow us to apply a selection criterion among isolates for further research.Fil: Ortega, Leonel Maximilano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Alconada Magliano, Teresa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Astoreca, Andrea Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Kikot, Gisele Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaHindawi Publishing Corporation2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4307Ortega, Leonel Maximilano; Alconada Magliano, Teresa Maria; Astoreca, Andrea Luciana; Kikot, Gisele Eleonora; Screening of Fusarium graminearum isolates for enzymes extracellular and deoxynivalenol production; Hindawi Publishing Corporation; Journal of Mycology; 2013; 10-2013; 358140-3581402314-6168enginfo:eu-repo/semantics/altIdentifier/url/http://www.hindawi.com/journals/jmy/2013/358140/info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/358140info:eu-repo/semantics/altIdentifier/issn/2314-6168info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:02Zoai:ri.conicet.gov.ar:11336/4307instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:02.377CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Screening of Fusarium graminearum isolates for enzymes extracellular and deoxynivalenol production
title Screening of Fusarium graminearum isolates for enzymes extracellular and deoxynivalenol production
spellingShingle Screening of Fusarium graminearum isolates for enzymes extracellular and deoxynivalenol production
Ortega, Leonel Maximilano
ENZYMES EXTRACELLULAR
DEOXYNIVALENOL
FUSARIUM GRAMINEARUM ISOLATES
SCREENING
title_short Screening of Fusarium graminearum isolates for enzymes extracellular and deoxynivalenol production
title_full Screening of Fusarium graminearum isolates for enzymes extracellular and deoxynivalenol production
title_fullStr Screening of Fusarium graminearum isolates for enzymes extracellular and deoxynivalenol production
title_full_unstemmed Screening of Fusarium graminearum isolates for enzymes extracellular and deoxynivalenol production
title_sort Screening of Fusarium graminearum isolates for enzymes extracellular and deoxynivalenol production
dc.creator.none.fl_str_mv Ortega, Leonel Maximilano
Alconada Magliano, Teresa Maria
Astoreca, Andrea Luciana
Kikot, Gisele Eleonora
author Ortega, Leonel Maximilano
author_facet Ortega, Leonel Maximilano
Alconada Magliano, Teresa Maria
Astoreca, Andrea Luciana
Kikot, Gisele Eleonora
author_role author
author2 Alconada Magliano, Teresa Maria
Astoreca, Andrea Luciana
Kikot, Gisele Eleonora
author2_role author
author
author
dc.subject.none.fl_str_mv ENZYMES EXTRACELLULAR
DEOXYNIVALENOL
FUSARIUM GRAMINEARUM ISOLATES
SCREENING
topic ENZYMES EXTRACELLULAR
DEOXYNIVALENOL
FUSARIUM GRAMINEARUM ISOLATES
SCREENING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fusarium graminearum, the main etiological agent of Fusarium Head Bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present report, several F. graminearum isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic and lipase extracellular enzymatic activities production, as well as by the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation with the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research, would allow us to apply a selection criterion among isolates for further research.
Fil: Ortega, Leonel Maximilano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Alconada Magliano, Teresa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Astoreca, Andrea Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Kikot, Gisele Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
description Fusarium graminearum, the main etiological agent of Fusarium Head Bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present report, several F. graminearum isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic and lipase extracellular enzymatic activities production, as well as by the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation with the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research, would allow us to apply a selection criterion among isolates for further research.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4307
Ortega, Leonel Maximilano; Alconada Magliano, Teresa Maria; Astoreca, Andrea Luciana; Kikot, Gisele Eleonora; Screening of Fusarium graminearum isolates for enzymes extracellular and deoxynivalenol production; Hindawi Publishing Corporation; Journal of Mycology; 2013; 10-2013; 358140-358140
2314-6168
url http://hdl.handle.net/11336/4307
identifier_str_mv Ortega, Leonel Maximilano; Alconada Magliano, Teresa Maria; Astoreca, Andrea Luciana; Kikot, Gisele Eleonora; Screening of Fusarium graminearum isolates for enzymes extracellular and deoxynivalenol production; Hindawi Publishing Corporation; Journal of Mycology; 2013; 10-2013; 358140-358140
2314-6168
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.hindawi.com/journals/jmy/2013/358140/
info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/358140
info:eu-repo/semantics/altIdentifier/issn/2314-6168
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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