Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean

Autores
Althabegoiti, María Julia; Covelli, Julieta Mariana; Pérez Giménez, Julieta; Quelas, Juan Ignacio; Mongiardini, Elías Javier; López, María Florencia; López García, Silvina Laura; Lodeiro, Aníbal Roberto
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bradyrhizobium japonicum has two types of flagella. One has thin filaments consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4 (FliC1-4). To investigate the roles of each flagellum in competition for nodulation, we obtained mutants deleted in fliCI-II and/or fliC1-4 in the genomic backgrounds of two derivatives from the reference strain USDA 110: the streptomycin-resistant derivative LP 3004 and its more motile derivative LP 3008. All mutations diminished swimming motility. When each mutant was co-inoculated with the parental strain on soybean plants cultivated in vermiculite either at field capacity or flooded, their competitiveness differed according to the flagellin altered. ΔfliCI-II mutants were more competitive, occupying 64-80% of the nodules, while ΔfliC1-4 mutants occupied 45-49% of the nodules. Occupation by the nonmotile double mutant decreased from 55% to 11% as the water content of the vermiculite increased from 85% to 95% field capacity to flooding. These results indicate that the influence of motility on competitiveness depended on the water status of the rooting substrate.
Instituto de Biotecnologia y Biologia Molecular
Materia
Biología
Flagellin
Soil
Swimming
Symbiosis
Water content
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/83919
Acceder
id SEDICI_589c32b650fd3142cc5d0aa0c9167ae9
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybeanAlthabegoiti, María JuliaCovelli, Julieta MarianaPérez Giménez, JulietaQuelas, Juan IgnacioMongiardini, Elías JavierLópez, María FlorenciaLópez García, Silvina LauraLodeiro, Aníbal RobertoBiologíaFlagellinSoilSwimmingSymbiosisWater contentBradyrhizobium japonicum has two types of flagella. One has thin filaments consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4 (FliC1-4). To investigate the roles of each flagellum in competition for nodulation, we obtained mutants deleted in fliCI-II and/or fliC1-4 in the genomic backgrounds of two derivatives from the reference strain USDA 110: the streptomycin-resistant derivative LP 3004 and its more motile derivative LP 3008. All mutations diminished swimming motility. When each mutant was co-inoculated with the parental strain on soybean plants cultivated in vermiculite either at field capacity or flooded, their competitiveness differed according to the flagellin altered. ΔfliCI-II mutants were more competitive, occupying 64-80% of the nodules, while ΔfliC1-4 mutants occupied 45-49% of the nodules. Occupation by the nonmotile double mutant decreased from 55% to 11% as the water content of the vermiculite increased from 85% to 95% field capacity to flooding. These results indicate that the influence of motility on competitiveness depended on the water status of the rooting substrate.Instituto de Biotecnologia y Biologia Molecular2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf133-139http://sedici.unlp.edu.ar/handle/10915/83919enginfo:eu-repo/semantics/altIdentifier/issn/0378-1097info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2011.02280.xinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:08:02Zoai:sedici.unlp.edu.ar:10915/83919Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:08:02.788SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
title Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
spellingShingle Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
Althabegoiti, María Julia
Biología
Flagellin
Soil
Swimming
Symbiosis
Water content
title_short Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
title_full Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
title_fullStr Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
title_full_unstemmed Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
title_sort Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
dc.creator.none.fl_str_mv Althabegoiti, María Julia
Covelli, Julieta Mariana
Pérez Giménez, Julieta
Quelas, Juan Ignacio
Mongiardini, Elías Javier
López, María Florencia
López García, Silvina Laura
Lodeiro, Aníbal Roberto
author Althabegoiti, María Julia
author_facet Althabegoiti, María Julia
Covelli, Julieta Mariana
Pérez Giménez, Julieta
Quelas, Juan Ignacio
Mongiardini, Elías Javier
López, María Florencia
López García, Silvina Laura
Lodeiro, Aníbal Roberto
author_role author
author2 Covelli, Julieta Mariana
Pérez Giménez, Julieta
Quelas, Juan Ignacio
Mongiardini, Elías Javier
López, María Florencia
López García, Silvina Laura
Lodeiro, Aníbal Roberto
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Flagellin
Soil
Swimming
Symbiosis
Water content
topic Biología
Flagellin
Soil
Swimming
Symbiosis
Water content
dc.description.none.fl_txt_mv Bradyrhizobium japonicum has two types of flagella. One has thin filaments consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4 (FliC1-4). To investigate the roles of each flagellum in competition for nodulation, we obtained mutants deleted in fliCI-II and/or fliC1-4 in the genomic backgrounds of two derivatives from the reference strain USDA 110: the streptomycin-resistant derivative LP 3004 and its more motile derivative LP 3008. All mutations diminished swimming motility. When each mutant was co-inoculated with the parental strain on soybean plants cultivated in vermiculite either at field capacity or flooded, their competitiveness differed according to the flagellin altered. ΔfliCI-II mutants were more competitive, occupying 64-80% of the nodules, while ΔfliC1-4 mutants occupied 45-49% of the nodules. Occupation by the nonmotile double mutant decreased from 55% to 11% as the water content of the vermiculite increased from 85% to 95% field capacity to flooding. These results indicate that the influence of motility on competitiveness depended on the water status of the rooting substrate.
Instituto de Biotecnologia y Biologia Molecular
description Bradyrhizobium japonicum has two types of flagella. One has thin filaments consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4 (FliC1-4). To investigate the roles of each flagellum in competition for nodulation, we obtained mutants deleted in fliCI-II and/or fliC1-4 in the genomic backgrounds of two derivatives from the reference strain USDA 110: the streptomycin-resistant derivative LP 3004 and its more motile derivative LP 3008. All mutations diminished swimming motility. When each mutant was co-inoculated with the parental strain on soybean plants cultivated in vermiculite either at field capacity or flooded, their competitiveness differed according to the flagellin altered. ΔfliCI-II mutants were more competitive, occupying 64-80% of the nodules, while ΔfliC1-4 mutants occupied 45-49% of the nodules. Occupation by the nonmotile double mutant decreased from 55% to 11% as the water content of the vermiculite increased from 85% to 95% field capacity to flooding. These results indicate that the influence of motility on competitiveness depended on the water status of the rooting substrate.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/83919
url http://sedici.unlp.edu.ar/handle/10915/83919
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0378-1097
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2011.02280.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
133-139
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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