Characterization of flil proteins in bradyrhizobium diazoefficiens: Lateral FLIL supports swimming motility, and subpolar FLIL modulates the lateral flagellar system

Autores
Mengucci, Florencia; Dardis, Carolina; Mongiardini, Elias Javier; Althabegoiti, Maria Julia; Partridge, Jonathan D.; Kojima, Seiji; Homma, Michio; Quelas, Juan Ignacio; Lodeiro, Anibal
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bradyrhizobium diazoefficiens is a soil alphaproteobacterium that possesses two evolutionarily distinct flagellar systems, a constitutive subpolar flagellum and inducible lateral flagella that, depending on the carbon source, may be expressed simultaneously in liquid medium and used interactively for swimming. In each system, more than 30 genes encode the flagellar proteins, most of which are well characterized. Among the exceptions is FliL, which has been scarcely studied in alphaproteobacteria and whose function in other bacterial classes is somewhat controversial. Because each B. diazoefficiens flagellar system contains its own fliL paralog, we obtained the respective deletions ΔfliLS (subpolar) and ΔfliLL (lateral) to study their functions in swimming. We determined that FliLL was essential for lateral flagellum-driven motility. FliLS was dispensable for swimming in either liquid or semisolid medium; however, it was found to play a crucial role in upregulation of the lateral flagellum regulon under conditions of increased viscosity/flagellar load. Therefore, although FliLS seems to be not essential for swimming, it may participate in a mechanosensor complex that controls lateral flagellum induction.
Fil: Mengucci, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Dardis, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Mongiardini, Elias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Althabegoiti, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Partridge, Jonathan D.. University of Texas at Austin; Estados Unidos
Fil: Kojima, Seiji. Nagoya University; Japón
Fil: Homma, Michio. Nagoya University; Japón
Fil: Quelas, Juan Ignacio. YPF - Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Lodeiro, Anibal. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
BRADYRHIZOBIUM
FLAGELLA
FLIL
SWIMMING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/159599
Acceder
id CONICETDig_1d2265d17f0621a3162f3791414ca22c
oai_identifier_str oai:ri.conicet.gov.ar:11336/159599
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of flil proteins in bradyrhizobium diazoefficiens: Lateral FLIL supports swimming motility, and subpolar FLIL modulates the lateral flagellar systemMengucci, FlorenciaDardis, CarolinaMongiardini, Elias JavierAlthabegoiti, Maria JuliaPartridge, Jonathan D.Kojima, SeijiHomma, MichioQuelas, Juan IgnacioLodeiro, AnibalBRADYRHIZOBIUMFLAGELLAFLILSWIMMINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bradyrhizobium diazoefficiens is a soil alphaproteobacterium that possesses two evolutionarily distinct flagellar systems, a constitutive subpolar flagellum and inducible lateral flagella that, depending on the carbon source, may be expressed simultaneously in liquid medium and used interactively for swimming. In each system, more than 30 genes encode the flagellar proteins, most of which are well characterized. Among the exceptions is FliL, which has been scarcely studied in alphaproteobacteria and whose function in other bacterial classes is somewhat controversial. Because each B. diazoefficiens flagellar system contains its own fliL paralog, we obtained the respective deletions ΔfliLS (subpolar) and ΔfliLL (lateral) to study their functions in swimming. We determined that FliLL was essential for lateral flagellum-driven motility. FliLS was dispensable for swimming in either liquid or semisolid medium; however, it was found to play a crucial role in upregulation of the lateral flagellum regulon under conditions of increased viscosity/flagellar load. Therefore, although FliLS seems to be not essential for swimming, it may participate in a mechanosensor complex that controls lateral flagellum induction.Fil: Mengucci, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Dardis, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Mongiardini, Elias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Althabegoiti, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Partridge, Jonathan D.. University of Texas at Austin; Estados UnidosFil: Kojima, Seiji. Nagoya University; JapónFil: Homma, Michio. Nagoya University; JapónFil: Quelas, Juan Ignacio. YPF - Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Lodeiro, Anibal. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaAmerican Society for Microbiology2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/159599Mengucci, Florencia; Dardis, Carolina; Mongiardini, Elias Javier; Althabegoiti, Maria Julia; Partridge, Jonathan D.; et al.; Characterization of flil proteins in bradyrhizobium diazoefficiens: Lateral FLIL supports swimming motility, and subpolar FLIL modulates the lateral flagellar system; American Society for Microbiology; Journal of Bacteriology; 202; 5; 12-2019; 1-150021-91931098-5530CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/JB.00708-19info:eu-repo/semantics/altIdentifier/doi/10.1128/JB.00708-19info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:21Zoai:ri.conicet.gov.ar:11336/159599instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:21.866CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of flil proteins in bradyrhizobium diazoefficiens: Lateral FLIL supports swimming motility, and subpolar FLIL modulates the lateral flagellar system
title Characterization of flil proteins in bradyrhizobium diazoefficiens: Lateral FLIL supports swimming motility, and subpolar FLIL modulates the lateral flagellar system
spellingShingle Characterization of flil proteins in bradyrhizobium diazoefficiens: Lateral FLIL supports swimming motility, and subpolar FLIL modulates the lateral flagellar system
Mengucci, Florencia
BRADYRHIZOBIUM
FLAGELLA
FLIL
SWIMMING
title_short Characterization of flil proteins in bradyrhizobium diazoefficiens: Lateral FLIL supports swimming motility, and subpolar FLIL modulates the lateral flagellar system
title_full Characterization of flil proteins in bradyrhizobium diazoefficiens: Lateral FLIL supports swimming motility, and subpolar FLIL modulates the lateral flagellar system
title_fullStr Characterization of flil proteins in bradyrhizobium diazoefficiens: Lateral FLIL supports swimming motility, and subpolar FLIL modulates the lateral flagellar system
title_full_unstemmed Characterization of flil proteins in bradyrhizobium diazoefficiens: Lateral FLIL supports swimming motility, and subpolar FLIL modulates the lateral flagellar system
title_sort Characterization of flil proteins in bradyrhizobium diazoefficiens: Lateral FLIL supports swimming motility, and subpolar FLIL modulates the lateral flagellar system
dc.creator.none.fl_str_mv Mengucci, Florencia
Dardis, Carolina
Mongiardini, Elias Javier
Althabegoiti, Maria Julia
Partridge, Jonathan D.
Kojima, Seiji
Homma, Michio
Quelas, Juan Ignacio
Lodeiro, Anibal
author Mengucci, Florencia
author_facet Mengucci, Florencia
Dardis, Carolina
Mongiardini, Elias Javier
Althabegoiti, Maria Julia
Partridge, Jonathan D.
Kojima, Seiji
Homma, Michio
Quelas, Juan Ignacio
Lodeiro, Anibal
author_role author
author2 Dardis, Carolina
Mongiardini, Elias Javier
Althabegoiti, Maria Julia
Partridge, Jonathan D.
Kojima, Seiji
Homma, Michio
Quelas, Juan Ignacio
Lodeiro, Anibal
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BRADYRHIZOBIUM
FLAGELLA
FLIL
SWIMMING
topic BRADYRHIZOBIUM
FLAGELLA
FLIL
SWIMMING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bradyrhizobium diazoefficiens is a soil alphaproteobacterium that possesses two evolutionarily distinct flagellar systems, a constitutive subpolar flagellum and inducible lateral flagella that, depending on the carbon source, may be expressed simultaneously in liquid medium and used interactively for swimming. In each system, more than 30 genes encode the flagellar proteins, most of which are well characterized. Among the exceptions is FliL, which has been scarcely studied in alphaproteobacteria and whose function in other bacterial classes is somewhat controversial. Because each B. diazoefficiens flagellar system contains its own fliL paralog, we obtained the respective deletions ΔfliLS (subpolar) and ΔfliLL (lateral) to study their functions in swimming. We determined that FliLL was essential for lateral flagellum-driven motility. FliLS was dispensable for swimming in either liquid or semisolid medium; however, it was found to play a crucial role in upregulation of the lateral flagellum regulon under conditions of increased viscosity/flagellar load. Therefore, although FliLS seems to be not essential for swimming, it may participate in a mechanosensor complex that controls lateral flagellum induction.
Fil: Mengucci, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Dardis, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Mongiardini, Elias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Althabegoiti, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Partridge, Jonathan D.. University of Texas at Austin; Estados Unidos
Fil: Kojima, Seiji. Nagoya University; Japón
Fil: Homma, Michio. Nagoya University; Japón
Fil: Quelas, Juan Ignacio. YPF - Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Lodeiro, Anibal. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description Bradyrhizobium diazoefficiens is a soil alphaproteobacterium that possesses two evolutionarily distinct flagellar systems, a constitutive subpolar flagellum and inducible lateral flagella that, depending on the carbon source, may be expressed simultaneously in liquid medium and used interactively for swimming. In each system, more than 30 genes encode the flagellar proteins, most of which are well characterized. Among the exceptions is FliL, which has been scarcely studied in alphaproteobacteria and whose function in other bacterial classes is somewhat controversial. Because each B. diazoefficiens flagellar system contains its own fliL paralog, we obtained the respective deletions ΔfliLS (subpolar) and ΔfliLL (lateral) to study their functions in swimming. We determined that FliLL was essential for lateral flagellum-driven motility. FliLS was dispensable for swimming in either liquid or semisolid medium; however, it was found to play a crucial role in upregulation of the lateral flagellum regulon under conditions of increased viscosity/flagellar load. Therefore, although FliLS seems to be not essential for swimming, it may participate in a mechanosensor complex that controls lateral flagellum induction.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/159599
Mengucci, Florencia; Dardis, Carolina; Mongiardini, Elias Javier; Althabegoiti, Maria Julia; Partridge, Jonathan D.; et al.; Characterization of flil proteins in bradyrhizobium diazoefficiens: Lateral FLIL supports swimming motility, and subpolar FLIL modulates the lateral flagellar system; American Society for Microbiology; Journal of Bacteriology; 202; 5; 12-2019; 1-15
0021-9193
1098-5530
CONICET Digital
CONICET
url http://hdl.handle.net/11336/159599
identifier_str_mv Mengucci, Florencia; Dardis, Carolina; Mongiardini, Elias Javier; Althabegoiti, Maria Julia; Partridge, Jonathan D.; et al.; Characterization of flil proteins in bradyrhizobium diazoefficiens: Lateral FLIL supports swimming motility, and subpolar FLIL modulates the lateral flagellar system; American Society for Microbiology; Journal of Bacteriology; 202; 5; 12-2019; 1-15
0021-9193
1098-5530
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/JB.00708-19
info:eu-repo/semantics/altIdentifier/doi/10.1128/JB.00708-19
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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