Swarming motility in Bradyrhizobium japonicum

Autores
Covelli, Julieta Mariana; Althabegoiti, Maria Julia; López, María Florencia; Lodeiro, Anibal
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Flagellar-driven bacterial motility is an important trait for colonization of natural environments. Bradyrhizobium japonicum is a soil speciesthat possesses two different flagellar systems: one subpolar and the other lateral, each with a filament formed by a different set of flagellins.While synthesis of subpolar flagellins is constitutive, translation of lateral flagellins was detected in rhizobia grown with L-arabinose, but notwith D-mannitol as sole carbon source, independently of whether bacteria were in liquid or semisolid medium. We characterized swarming of B.japonicum in semisolid medium and found that this motility was faster with L-arabinose than with D-mannitol. By using mutants with deletions ineach flagellin set, we evaluated the contribution of each flagellum system to swarming in semisolid culture media, and in soil. Mutants devoid ofeither of the flagella were affected in swarming in culture media, with this impairment being stronger for mutants without lateral flagella. Insterile soil at 100% or 80% field capacity, flagellar-driven motility of mutants able to swim but impaired in swarming was similar to wild type,indicating that swimming was the predominant movement here.
Fil: Covelli, Julieta Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Althabegoiti, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: López, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Lodeiro, Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
Bradyrhizobium
Swimming
Swarming
Flagellum
Soil
Rhizosphere
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/271185
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/271185
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Swarming motility in Bradyrhizobium japonicumCovelli, Julieta MarianaAlthabegoiti, Maria JuliaLópez, María FlorenciaLodeiro, AnibalBradyrhizobiumSwimmingSwarmingFlagellumSoilRhizospherehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Flagellar-driven bacterial motility is an important trait for colonization of natural environments. Bradyrhizobium japonicum is a soil speciesthat possesses two different flagellar systems: one subpolar and the other lateral, each with a filament formed by a different set of flagellins.While synthesis of subpolar flagellins is constitutive, translation of lateral flagellins was detected in rhizobia grown with L-arabinose, but notwith D-mannitol as sole carbon source, independently of whether bacteria were in liquid or semisolid medium. We characterized swarming of B.japonicum in semisolid medium and found that this motility was faster with L-arabinose than with D-mannitol. By using mutants with deletions ineach flagellin set, we evaluated the contribution of each flagellum system to swarming in semisolid culture media, and in soil. Mutants devoid ofeither of the flagella were affected in swarming in culture media, with this impairment being stronger for mutants without lateral flagella. Insterile soil at 100% or 80% field capacity, flagellar-driven motility of mutants able to swim but impaired in swarming was similar to wild type,indicating that swimming was the predominant movement here.Fil: Covelli, Julieta Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Althabegoiti, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: López, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Lodeiro, Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaElsevier Science2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271185Covelli, Julieta Mariana; Althabegoiti, Maria Julia; López, María Florencia; Lodeiro, Anibal; Swarming motility in Bradyrhizobium japonicum; Elsevier Science; Research In Microbiology; 164; 2; 2-2013; 136-1440923-2508CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0923250812001593info:eu-repo/semantics/altIdentifier/doi/10.1016/j.resmic.2012.10.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:49Zoai:ri.conicet.gov.ar:11336/271185instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:50.16CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Swarming motility in Bradyrhizobium japonicum
title Swarming motility in Bradyrhizobium japonicum
spellingShingle Swarming motility in Bradyrhizobium japonicum
Covelli, Julieta Mariana
Bradyrhizobium
Swimming
Swarming
Flagellum
Soil
Rhizosphere
title_short Swarming motility in Bradyrhizobium japonicum
title_full Swarming motility in Bradyrhizobium japonicum
title_fullStr Swarming motility in Bradyrhizobium japonicum
title_full_unstemmed Swarming motility in Bradyrhizobium japonicum
title_sort Swarming motility in Bradyrhizobium japonicum
dc.creator.none.fl_str_mv Covelli, Julieta Mariana
Althabegoiti, Maria Julia
López, María Florencia
Lodeiro, Anibal
author Covelli, Julieta Mariana
author_facet Covelli, Julieta Mariana
Althabegoiti, Maria Julia
López, María Florencia
Lodeiro, Anibal
author_role author
author2 Althabegoiti, Maria Julia
López, María Florencia
Lodeiro, Anibal
author2_role author
author
author
dc.subject.none.fl_str_mv Bradyrhizobium
Swimming
Swarming
Flagellum
Soil
Rhizosphere
topic Bradyrhizobium
Swimming
Swarming
Flagellum
Soil
Rhizosphere
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Flagellar-driven bacterial motility is an important trait for colonization of natural environments. Bradyrhizobium japonicum is a soil speciesthat possesses two different flagellar systems: one subpolar and the other lateral, each with a filament formed by a different set of flagellins.While synthesis of subpolar flagellins is constitutive, translation of lateral flagellins was detected in rhizobia grown with L-arabinose, but notwith D-mannitol as sole carbon source, independently of whether bacteria were in liquid or semisolid medium. We characterized swarming of B.japonicum in semisolid medium and found that this motility was faster with L-arabinose than with D-mannitol. By using mutants with deletions ineach flagellin set, we evaluated the contribution of each flagellum system to swarming in semisolid culture media, and in soil. Mutants devoid ofeither of the flagella were affected in swarming in culture media, with this impairment being stronger for mutants without lateral flagella. Insterile soil at 100% or 80% field capacity, flagellar-driven motility of mutants able to swim but impaired in swarming was similar to wild type,indicating that swimming was the predominant movement here.
Fil: Covelli, Julieta Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Althabegoiti, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: López, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Lodeiro, Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description Flagellar-driven bacterial motility is an important trait for colonization of natural environments. Bradyrhizobium japonicum is a soil speciesthat possesses two different flagellar systems: one subpolar and the other lateral, each with a filament formed by a different set of flagellins.While synthesis of subpolar flagellins is constitutive, translation of lateral flagellins was detected in rhizobia grown with L-arabinose, but notwith D-mannitol as sole carbon source, independently of whether bacteria were in liquid or semisolid medium. We characterized swarming of B.japonicum in semisolid medium and found that this motility was faster with L-arabinose than with D-mannitol. By using mutants with deletions ineach flagellin set, we evaluated the contribution of each flagellum system to swarming in semisolid culture media, and in soil. Mutants devoid ofeither of the flagella were affected in swarming in culture media, with this impairment being stronger for mutants without lateral flagella. Insterile soil at 100% or 80% field capacity, flagellar-driven motility of mutants able to swim but impaired in swarming was similar to wild type,indicating that swimming was the predominant movement here.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/271185
Covelli, Julieta Mariana; Althabegoiti, Maria Julia; López, María Florencia; Lodeiro, Anibal; Swarming motility in Bradyrhizobium japonicum; Elsevier Science; Research In Microbiology; 164; 2; 2-2013; 136-144
0923-2508
CONICET Digital
CONICET
url http://hdl.handle.net/11336/271185
identifier_str_mv Covelli, Julieta Mariana; Althabegoiti, Maria Julia; López, María Florencia; Lodeiro, Anibal; Swarming motility in Bradyrhizobium japonicum; Elsevier Science; Research In Microbiology; 164; 2; 2-2013; 136-144
0923-2508
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0923250812001593
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.resmic.2012.10.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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