Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency

Autores
Gaillard, María Emilia; Bottero, Daniela; Zurita, María Eugenia; Carriquiriborde, Francisco; Aispuro, Pablo Martín; Bartel, Erika; Sabater Martínez, David; Bravo, María Sol; Castuma, Cecilia; Hozbor, Daniela Flavia
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Maternal safety through pertussis vaccination and subsequent maternal–fetal-antibody transfer are well documented, but information on infant protection from pertussis by such antibodies and by subsequent vaccinations is scarce. Since mice are used extensively for maternal-vaccination studies, we adopted that model to narrow those gaps in our understanding of maternal pertussis immunization. Accordingly, we vaccinated female mice with commercial acellular pertussis (aP) vaccine and measured offspring protection against Bordetella pertussis challenge and specific-antibody levels with or without revaccination. Maternal immunization protected the offspring against pertussis, with that immune protection transferred to the offspring lasting for several weeks, as evidenced by a reduction (4–5 logs, p < 0.001) in the colony-forming-units recovered from the lungs of 16-week-old offspring. Moreover, maternal-vaccination-acquired immunity from the first pregnancy still conferred protection to offspring up to the fourth pregnancy. Under the conditions of our experimental protocol, protection to offspring from the aP-induced immunity is transferred both transplacentally and through breastfeeding. Adoptive-transfer experiments demonstrated that transferred antibodies were more responsible for the protection detected in offspring than transferred whole spleen cells. In contrast to reported findings, the protection transferred was not lost after the vaccination of infant mice with the same or other vaccine preparations, and conversely, the immunity transferred from mothers did not interfere with the protection conferred by infant vaccination with the same or different vaccines. These results indicated that aP-vaccine immunization of pregnant female mice conferred protective immunity that is transferred both transplacentally and via offspring breastfeeding without compromising the protection boostered by subsequent infant vaccination. These results—though admittedly not necessarily immediately extrapolatable to humans—nevertheless enabled us to test hypotheses under controlled conditions through detailed sampling and data collection. These findings will hopefully refine hypotheses that can then be validated in subsequent human studies.
Facultad de Ciencias Exactas
Materia
Biología
Bordetella pertussis
pregnancy immunization
acellular vaccine
protection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/78156

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spelling Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination FrequencyGaillard, María EmiliaBottero, DanielaZurita, María EugeniaCarriquiriborde, FranciscoAispuro, Pablo MartínBartel, ErikaSabater Martínez, DavidBravo, María SolCastuma, CeciliaHozbor, Daniela FlaviaBiologíaBordetella pertussispregnancy immunizationacellular vaccineprotectionMaternal safety through pertussis vaccination and subsequent maternal–fetal-antibody transfer are well documented, but information on infant protection from pertussis by such antibodies and by subsequent vaccinations is scarce. Since mice are used extensively for maternal-vaccination studies, we adopted that model to narrow those gaps in our understanding of maternal pertussis immunization. Accordingly, we vaccinated female mice with commercial acellular pertussis (aP) vaccine and measured offspring protection against Bordetella pertussis challenge and specific-antibody levels with or without revaccination. Maternal immunization protected the offspring against pertussis, with that immune protection transferred to the offspring lasting for several weeks, as evidenced by a reduction (4–5 logs, p &lt; 0.001) in the colony-forming-units recovered from the lungs of 16-week-old offspring. Moreover, maternal-vaccination-acquired immunity from the first pregnancy still conferred protection to offspring up to the fourth pregnancy. Under the conditions of our experimental protocol, protection to offspring from the aP-induced immunity is transferred both transplacentally and through breastfeeding. Adoptive-transfer experiments demonstrated that transferred antibodies were more responsible for the protection detected in offspring than transferred whole spleen cells. In contrast to reported findings, the protection transferred was not lost after the vaccination of infant mice with the same or other vaccine preparations, and conversely, the immunity transferred from mothers did not interfere with the protection conferred by infant vaccination with the same or different vaccines. These results indicated that aP-vaccine immunization of pregnant female mice conferred protective immunity that is transferred both transplacentally and via offspring breastfeeding without compromising the protection boostered by subsequent infant vaccination. These results—though admittedly not necessarily immediately extrapolatable to humans—nevertheless enabled us to test hypotheses under controlled conditions through detailed sampling and data collection. These findings will hopefully refine hypotheses that can then be validated in subsequent human studies.Facultad de Ciencias Exactas2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/78156enginfo:eu-repo/semantics/altIdentifier/issn/1664-3224info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2017.01099info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:14:03Zoai:sedici.unlp.edu.ar:10915/78156Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:14:03.989SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency
title Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency
spellingShingle Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency
Gaillard, María Emilia
Biología
Bordetella pertussis
pregnancy immunization
acellular vaccine
protection
title_short Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency
title_full Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency
title_fullStr Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency
title_full_unstemmed Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency
title_sort Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency
dc.creator.none.fl_str_mv Gaillard, María Emilia
Bottero, Daniela
Zurita, María Eugenia
Carriquiriborde, Francisco
Aispuro, Pablo Martín
Bartel, Erika
Sabater Martínez, David
Bravo, María Sol
Castuma, Cecilia
Hozbor, Daniela Flavia
author Gaillard, María Emilia
author_facet Gaillard, María Emilia
Bottero, Daniela
Zurita, María Eugenia
Carriquiriborde, Francisco
Aispuro, Pablo Martín
Bartel, Erika
Sabater Martínez, David
Bravo, María Sol
Castuma, Cecilia
Hozbor, Daniela Flavia
author_role author
author2 Bottero, Daniela
Zurita, María Eugenia
Carriquiriborde, Francisco
Aispuro, Pablo Martín
Bartel, Erika
Sabater Martínez, David
Bravo, María Sol
Castuma, Cecilia
Hozbor, Daniela Flavia
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Bordetella pertussis
pregnancy immunization
acellular vaccine
protection
topic Biología
Bordetella pertussis
pregnancy immunization
acellular vaccine
protection
dc.description.none.fl_txt_mv Maternal safety through pertussis vaccination and subsequent maternal–fetal-antibody transfer are well documented, but information on infant protection from pertussis by such antibodies and by subsequent vaccinations is scarce. Since mice are used extensively for maternal-vaccination studies, we adopted that model to narrow those gaps in our understanding of maternal pertussis immunization. Accordingly, we vaccinated female mice with commercial acellular pertussis (aP) vaccine and measured offspring protection against Bordetella pertussis challenge and specific-antibody levels with or without revaccination. Maternal immunization protected the offspring against pertussis, with that immune protection transferred to the offspring lasting for several weeks, as evidenced by a reduction (4–5 logs, p &lt; 0.001) in the colony-forming-units recovered from the lungs of 16-week-old offspring. Moreover, maternal-vaccination-acquired immunity from the first pregnancy still conferred protection to offspring up to the fourth pregnancy. Under the conditions of our experimental protocol, protection to offspring from the aP-induced immunity is transferred both transplacentally and through breastfeeding. Adoptive-transfer experiments demonstrated that transferred antibodies were more responsible for the protection detected in offspring than transferred whole spleen cells. In contrast to reported findings, the protection transferred was not lost after the vaccination of infant mice with the same or other vaccine preparations, and conversely, the immunity transferred from mothers did not interfere with the protection conferred by infant vaccination with the same or different vaccines. These results indicated that aP-vaccine immunization of pregnant female mice conferred protective immunity that is transferred both transplacentally and via offspring breastfeeding without compromising the protection boostered by subsequent infant vaccination. These results—though admittedly not necessarily immediately extrapolatable to humans—nevertheless enabled us to test hypotheses under controlled conditions through detailed sampling and data collection. These findings will hopefully refine hypotheses that can then be validated in subsequent human studies.
Facultad de Ciencias Exactas
description Maternal safety through pertussis vaccination and subsequent maternal–fetal-antibody transfer are well documented, but information on infant protection from pertussis by such antibodies and by subsequent vaccinations is scarce. Since mice are used extensively for maternal-vaccination studies, we adopted that model to narrow those gaps in our understanding of maternal pertussis immunization. Accordingly, we vaccinated female mice with commercial acellular pertussis (aP) vaccine and measured offspring protection against Bordetella pertussis challenge and specific-antibody levels with or without revaccination. Maternal immunization protected the offspring against pertussis, with that immune protection transferred to the offspring lasting for several weeks, as evidenced by a reduction (4–5 logs, p &lt; 0.001) in the colony-forming-units recovered from the lungs of 16-week-old offspring. Moreover, maternal-vaccination-acquired immunity from the first pregnancy still conferred protection to offspring up to the fourth pregnancy. Under the conditions of our experimental protocol, protection to offspring from the aP-induced immunity is transferred both transplacentally and through breastfeeding. Adoptive-transfer experiments demonstrated that transferred antibodies were more responsible for the protection detected in offspring than transferred whole spleen cells. In contrast to reported findings, the protection transferred was not lost after the vaccination of infant mice with the same or other vaccine preparations, and conversely, the immunity transferred from mothers did not interfere with the protection conferred by infant vaccination with the same or different vaccines. These results indicated that aP-vaccine immunization of pregnant female mice conferred protective immunity that is transferred both transplacentally and via offspring breastfeeding without compromising the protection boostered by subsequent infant vaccination. These results—though admittedly not necessarily immediately extrapolatable to humans—nevertheless enabled us to test hypotheses under controlled conditions through detailed sampling and data collection. These findings will hopefully refine hypotheses that can then be validated in subsequent human studies.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
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info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2017.01099
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