Assessment of bacterial inoculant formulated with <i>Paraburkholderia tropica</i> to enhance wheat productivity

Autores
Bernabeu, Pamela Romina; García, Sabrina Soledad; López, Ana Clara; Vio, Santiago Adolfo; Carrasco, Natalia; Boiardi, José Luis; Luna, María Flavia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Paraburkholderia tropica is an endophytic nitrogen-fixing bacterium isolated from the rhizosphere, rhizoplane, and internal tissues of sugarcane and corn plants in different geographical regions. Other plant-growth-promoting abilities, such as phosphate solubilization and antifungal activity, have also been reported for this bacterium. With an aim at investigating the potential use of P. tropica as an inoculant for improving the performance of wheat crop, in this work we evaluated an experimental inoculant formulated with P. tropica MTo-293 with respect to root colonization, the practical aspects of its application, and the effects under field conditions when applied to wheat seeds. Bacterial colonization was monitored by culture dependent techniques and the wheat yield determined by quantifying the total grain production in two different seasons. Rhizoplane and endophytic colonization in wheat roots was achieved efficiently (on average, 8 and 4 log colony-forming units/g fresh weight, respectively) even at relatively low concentrations of viable bacteria in the inoculum under controlled conditions. P. tropica was compatible with a widely used fungicide, maintained viability for 48 h once applied to seeds, and was also able to colonize wheat roots efficiently. Furthermore, we were able to formulate an inoculant that maintained bacterial viability for relatively long time periods. Preliminary field assays were realized, and even though the average yields values for the inoculated treatments remained above the uninoculated ones, no significant effects of inoculation were detected with or without fertilization. The correct physiologic behavior of P. tropica suggests the necessity to continue with field experiments under different conditions.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
Biología
Endophytes
Paraburkholderia
Plant growth promotion
Seed treatment
Wheat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/144589

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network_name_str SEDICI (UNLP)
spelling Assessment of bacterial inoculant formulated with <i>Paraburkholderia tropica</i> to enhance wheat productivityBernabeu, Pamela RominaGarcía, Sabrina SoledadLópez, Ana ClaraVio, Santiago AdolfoCarrasco, NataliaBoiardi, José LuisLuna, María FlaviaCiencias ExactasBiologíaEndophytesParaburkholderiaPlant growth promotionSeed treatmentWheat<i>Paraburkholderia tropica</i> is an endophytic nitrogen-fixing bacterium isolated from the rhizosphere, rhizoplane, and internal tissues of sugarcane and corn plants in different geographical regions. Other plant-growth-promoting abilities, such as phosphate solubilization and antifungal activity, have also been reported for this bacterium. With an aim at investigating the potential use of <i>P. tropica</i> as an inoculant for improving the performance of wheat crop, in this work we evaluated an experimental inoculant formulated with <i>P. tropica</i> MTo-293 with respect to root colonization, the practical aspects of its application, and the effects under field conditions when applied to wheat seeds. Bacterial colonization was monitored by culture dependent techniques and the wheat yield determined by quantifying the total grain production in two different seasons. Rhizoplane and endophytic colonization in wheat roots was achieved efficiently (on average, 8 and 4 log colony-forming units/g fresh weight, respectively) even at relatively low concentrations of viable bacteria in the inoculum under controlled conditions. <i>P. tropica</i> was compatible with a widely used fungicide, maintained viability for 48 h once applied to seeds, and was also able to colonize wheat roots efficiently. Furthermore, we were able to formulate an inoculant that maintained bacterial viability for relatively long time periods. Preliminary field assays were realized, and even though the average yields values for the inoculated treatments remained above the uninoculated ones, no significant effects of inoculation were detected with or without fertilization. The correct physiologic behavior of <i>P. tropica</i> suggests the necessity to continue with field experiments under different conditions.Centro de Investigación y Desarrollo en Fermentaciones Industriales2018-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/144589enginfo:eu-repo/semantics/altIdentifier/issn/1573-0972info:eu-repo/semantics/altIdentifier/issn/0959-3993info:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-018-2461-4info:eu-repo/semantics/altIdentifier/pmid/29802598info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:22Zoai:sedici.unlp.edu.ar:10915/144589Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:23.055SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Assessment of bacterial inoculant formulated with <i>Paraburkholderia tropica</i> to enhance wheat productivity
title Assessment of bacterial inoculant formulated with <i>Paraburkholderia tropica</i> to enhance wheat productivity
spellingShingle Assessment of bacterial inoculant formulated with <i>Paraburkholderia tropica</i> to enhance wheat productivity
Bernabeu, Pamela Romina
Ciencias Exactas
Biología
Endophytes
Paraburkholderia
Plant growth promotion
Seed treatment
Wheat
title_short Assessment of bacterial inoculant formulated with <i>Paraburkholderia tropica</i> to enhance wheat productivity
title_full Assessment of bacterial inoculant formulated with <i>Paraburkholderia tropica</i> to enhance wheat productivity
title_fullStr Assessment of bacterial inoculant formulated with <i>Paraburkholderia tropica</i> to enhance wheat productivity
title_full_unstemmed Assessment of bacterial inoculant formulated with <i>Paraburkholderia tropica</i> to enhance wheat productivity
title_sort Assessment of bacterial inoculant formulated with <i>Paraburkholderia tropica</i> to enhance wheat productivity
dc.creator.none.fl_str_mv Bernabeu, Pamela Romina
García, Sabrina Soledad
López, Ana Clara
Vio, Santiago Adolfo
Carrasco, Natalia
Boiardi, José Luis
Luna, María Flavia
author Bernabeu, Pamela Romina
author_facet Bernabeu, Pamela Romina
García, Sabrina Soledad
López, Ana Clara
Vio, Santiago Adolfo
Carrasco, Natalia
Boiardi, José Luis
Luna, María Flavia
author_role author
author2 García, Sabrina Soledad
López, Ana Clara
Vio, Santiago Adolfo
Carrasco, Natalia
Boiardi, José Luis
Luna, María Flavia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
Endophytes
Paraburkholderia
Plant growth promotion
Seed treatment
Wheat
topic Ciencias Exactas
Biología
Endophytes
Paraburkholderia
Plant growth promotion
Seed treatment
Wheat
dc.description.none.fl_txt_mv <i>Paraburkholderia tropica</i> is an endophytic nitrogen-fixing bacterium isolated from the rhizosphere, rhizoplane, and internal tissues of sugarcane and corn plants in different geographical regions. Other plant-growth-promoting abilities, such as phosphate solubilization and antifungal activity, have also been reported for this bacterium. With an aim at investigating the potential use of <i>P. tropica</i> as an inoculant for improving the performance of wheat crop, in this work we evaluated an experimental inoculant formulated with <i>P. tropica</i> MTo-293 with respect to root colonization, the practical aspects of its application, and the effects under field conditions when applied to wheat seeds. Bacterial colonization was monitored by culture dependent techniques and the wheat yield determined by quantifying the total grain production in two different seasons. Rhizoplane and endophytic colonization in wheat roots was achieved efficiently (on average, 8 and 4 log colony-forming units/g fresh weight, respectively) even at relatively low concentrations of viable bacteria in the inoculum under controlled conditions. <i>P. tropica</i> was compatible with a widely used fungicide, maintained viability for 48 h once applied to seeds, and was also able to colonize wheat roots efficiently. Furthermore, we were able to formulate an inoculant that maintained bacterial viability for relatively long time periods. Preliminary field assays were realized, and even though the average yields values for the inoculated treatments remained above the uninoculated ones, no significant effects of inoculation were detected with or without fertilization. The correct physiologic behavior of <i>P. tropica</i> suggests the necessity to continue with field experiments under different conditions.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description <i>Paraburkholderia tropica</i> is an endophytic nitrogen-fixing bacterium isolated from the rhizosphere, rhizoplane, and internal tissues of sugarcane and corn plants in different geographical regions. Other plant-growth-promoting abilities, such as phosphate solubilization and antifungal activity, have also been reported for this bacterium. With an aim at investigating the potential use of <i>P. tropica</i> as an inoculant for improving the performance of wheat crop, in this work we evaluated an experimental inoculant formulated with <i>P. tropica</i> MTo-293 with respect to root colonization, the practical aspects of its application, and the effects under field conditions when applied to wheat seeds. Bacterial colonization was monitored by culture dependent techniques and the wheat yield determined by quantifying the total grain production in two different seasons. Rhizoplane and endophytic colonization in wheat roots was achieved efficiently (on average, 8 and 4 log colony-forming units/g fresh weight, respectively) even at relatively low concentrations of viable bacteria in the inoculum under controlled conditions. <i>P. tropica</i> was compatible with a widely used fungicide, maintained viability for 48 h once applied to seeds, and was also able to colonize wheat roots efficiently. Furthermore, we were able to formulate an inoculant that maintained bacterial viability for relatively long time periods. Preliminary field assays were realized, and even though the average yields values for the inoculated treatments remained above the uninoculated ones, no significant effects of inoculation were detected with or without fertilization. The correct physiologic behavior of <i>P. tropica</i> suggests the necessity to continue with field experiments under different conditions.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/144589
url http://sedici.unlp.edu.ar/handle/10915/144589
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1573-0972
info:eu-repo/semantics/altIdentifier/issn/0959-3993
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-018-2461-4
info:eu-repo/semantics/altIdentifier/pmid/29802598
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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