Genomic and Functional Characterization of a Novel Burkholderia sp. Strain AU4i from Pea Rhizosphere Conferring Plant Growth Promoting Activities

Autores
Usha, Devi; Khatri, Indu; Lalit, Kumar; Devender, Singh; Aditi, Gupta; Navinder, Kumar; Gárriz, Andrés; Subramanian, Srikrishna; Sharma, Deepak; Adesh, Saini K.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Members of Burkholderia genus are gaining importance for their application in crop improvement by acting as plant growth-promoting rhizobacteria (PGPR), however, our knowledge about mechanisms of their plant growth promotion is limited. Herein, we aimed to isolate Burkholderia sp. from pea rhizosphere that confers plant growth promoting traits both in vitro and in vivo using pea seedlings as model. We have isolated a novel Burkholderia strain AU4i (B-AU4i) from pea rhizosphere that strongly promotes root and shoot growth in plantae. B-AU4i confers phosphate solubilization, indole-3-acetic acid production, N2 fixation, ammonia production, siderophore production, HCN production, and inhibits growth of pathogenic fungi both in vitro and in vivo experiments where we employed pea-seedlings as model system. Sequencing of B-AU4i genome using the Illumina-HiSeq 1000 technology reveals that it contains genes for the above-mentioned biofertilizer and biocontrol activities. The findings demonstrate the potential use of B-AU4i as plant growth promoter, which could be due to the presence of relevant genes in its genome. The current study improves our knowledge regarding the genes present in Burkholderia spp. that are involved in its plant growth promotion activities. B-AU4i strain can be used for improving agriculture productivity owing to its strong biofertilizer and biocontrol activity.
Fil: Usha, Devi. Shoolini University; India
Fil: Khatri, Indu. Council of Scientific & Industrial Research. Institute of Microbial Technology; India
Fil: Lalit, Kumar. Shoolini University; India
Fil: Devender, Singh. Shoolini University; India
Fil: Aditi, Gupta. Shoolini University; India
Fil: Navinder, Kumar. Council of Scientific & Industrial Research. Institute of Microbial Technology; India
Fil: Gárriz, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Subramanian, Srikrishna. Council of Scientific & Industrial Research. Institute of Microbial Technology; India
Fil: Sharma, Deepak. Council of Scientific & Industrial Research. Institute of Microbial Technology; India
Fil: Adesh, Saini K.. Shoolini University; India
Materia
Endophytes
Burkholderia
Plant defense
Plant growth promotion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54023
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Genomic and Functional Characterization of a Novel Burkholderia sp. Strain AU4i from Pea Rhizosphere Conferring Plant Growth Promoting ActivitiesUsha, DeviKhatri, InduLalit, KumarDevender, SinghAditi, GuptaNavinder, KumarGárriz, AndrésSubramanian, SrikrishnaSharma, DeepakAdesh, Saini K.EndophytesBurkholderiaPlant defensePlant growth promotionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Members of Burkholderia genus are gaining importance for their application in crop improvement by acting as plant growth-promoting rhizobacteria (PGPR), however, our knowledge about mechanisms of their plant growth promotion is limited. Herein, we aimed to isolate Burkholderia sp. from pea rhizosphere that confers plant growth promoting traits both in vitro and in vivo using pea seedlings as model. We have isolated a novel Burkholderia strain AU4i (B-AU4i) from pea rhizosphere that strongly promotes root and shoot growth in plantae. B-AU4i confers phosphate solubilization, indole-3-acetic acid production, N2 fixation, ammonia production, siderophore production, HCN production, and inhibits growth of pathogenic fungi both in vitro and in vivo experiments where we employed pea-seedlings as model system. Sequencing of B-AU4i genome using the Illumina-HiSeq 1000 technology reveals that it contains genes for the above-mentioned biofertilizer and biocontrol activities. The findings demonstrate the potential use of B-AU4i as plant growth promoter, which could be due to the presence of relevant genes in its genome. The current study improves our knowledge regarding the genes present in Burkholderia spp. that are involved in its plant growth promotion activities. B-AU4i strain can be used for improving agriculture productivity owing to its strong biofertilizer and biocontrol activity.Fil: Usha, Devi. Shoolini University; IndiaFil: Khatri, Indu. Council of Scientific & Industrial Research. Institute of Microbial Technology; IndiaFil: Lalit, Kumar. Shoolini University; IndiaFil: Devender, Singh. Shoolini University; IndiaFil: Aditi, Gupta. Shoolini University; IndiaFil: Navinder, Kumar. Council of Scientific & Industrial Research. Institute of Microbial Technology; IndiaFil: Gárriz, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Subramanian, Srikrishna. Council of Scientific & Industrial Research. Institute of Microbial Technology; IndiaFil: Sharma, Deepak. Council of Scientific & Industrial Research. Institute of Microbial Technology; IndiaFil: Adesh, Saini K.. Shoolini University; IndiaOMICS International2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54023Usha, Devi; Khatri, Indu; Lalit, Kumar; Devender, Singh; Aditi, Gupta; et al.; Genomic and Functional Characterization of a Novel Burkholderia sp. Strain AU4i from Pea Rhizosphere Conferring Plant Growth Promoting Activities; OMICS International; Advancements in Genetic Engineering; 4; 3; 9-2015; 1-82169-0111CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4/2169-0111.1000129info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/genomic-and-functional-characterization-of-a-novel-burkholderia-sp-strainau4i-from-pea-rhizosphere-conferring-plant-growth-promoting-activities-2169-0111-1000129.php?aid=63316info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:23:15Zoai:ri.conicet.gov.ar:11336/54023instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:23:15.338CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genomic and Functional Characterization of a Novel Burkholderia sp. Strain AU4i from Pea Rhizosphere Conferring Plant Growth Promoting Activities
title Genomic and Functional Characterization of a Novel Burkholderia sp. Strain AU4i from Pea Rhizosphere Conferring Plant Growth Promoting Activities
spellingShingle Genomic and Functional Characterization of a Novel Burkholderia sp. Strain AU4i from Pea Rhizosphere Conferring Plant Growth Promoting Activities
Usha, Devi
Endophytes
Burkholderia
Plant defense
Plant growth promotion
title_short Genomic and Functional Characterization of a Novel Burkholderia sp. Strain AU4i from Pea Rhizosphere Conferring Plant Growth Promoting Activities
title_full Genomic and Functional Characterization of a Novel Burkholderia sp. Strain AU4i from Pea Rhizosphere Conferring Plant Growth Promoting Activities
title_fullStr Genomic and Functional Characterization of a Novel Burkholderia sp. Strain AU4i from Pea Rhizosphere Conferring Plant Growth Promoting Activities
title_full_unstemmed Genomic and Functional Characterization of a Novel Burkholderia sp. Strain AU4i from Pea Rhizosphere Conferring Plant Growth Promoting Activities
title_sort Genomic and Functional Characterization of a Novel Burkholderia sp. Strain AU4i from Pea Rhizosphere Conferring Plant Growth Promoting Activities
dc.creator.none.fl_str_mv Usha, Devi
Khatri, Indu
Lalit, Kumar
Devender, Singh
Aditi, Gupta
Navinder, Kumar
Gárriz, Andrés
Subramanian, Srikrishna
Sharma, Deepak
Adesh, Saini K.
author Usha, Devi
author_facet Usha, Devi
Khatri, Indu
Lalit, Kumar
Devender, Singh
Aditi, Gupta
Navinder, Kumar
Gárriz, Andrés
Subramanian, Srikrishna
Sharma, Deepak
Adesh, Saini K.
author_role author
author2 Khatri, Indu
Lalit, Kumar
Devender, Singh
Aditi, Gupta
Navinder, Kumar
Gárriz, Andrés
Subramanian, Srikrishna
Sharma, Deepak
Adesh, Saini K.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Endophytes
Burkholderia
Plant defense
Plant growth promotion
topic Endophytes
Burkholderia
Plant defense
Plant growth promotion
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Members of Burkholderia genus are gaining importance for their application in crop improvement by acting as plant growth-promoting rhizobacteria (PGPR), however, our knowledge about mechanisms of their plant growth promotion is limited. Herein, we aimed to isolate Burkholderia sp. from pea rhizosphere that confers plant growth promoting traits both in vitro and in vivo using pea seedlings as model. We have isolated a novel Burkholderia strain AU4i (B-AU4i) from pea rhizosphere that strongly promotes root and shoot growth in plantae. B-AU4i confers phosphate solubilization, indole-3-acetic acid production, N2 fixation, ammonia production, siderophore production, HCN production, and inhibits growth of pathogenic fungi both in vitro and in vivo experiments where we employed pea-seedlings as model system. Sequencing of B-AU4i genome using the Illumina-HiSeq 1000 technology reveals that it contains genes for the above-mentioned biofertilizer and biocontrol activities. The findings demonstrate the potential use of B-AU4i as plant growth promoter, which could be due to the presence of relevant genes in its genome. The current study improves our knowledge regarding the genes present in Burkholderia spp. that are involved in its plant growth promotion activities. B-AU4i strain can be used for improving agriculture productivity owing to its strong biofertilizer and biocontrol activity.
Fil: Usha, Devi. Shoolini University; India
Fil: Khatri, Indu. Council of Scientific & Industrial Research. Institute of Microbial Technology; India
Fil: Lalit, Kumar. Shoolini University; India
Fil: Devender, Singh. Shoolini University; India
Fil: Aditi, Gupta. Shoolini University; India
Fil: Navinder, Kumar. Council of Scientific & Industrial Research. Institute of Microbial Technology; India
Fil: Gárriz, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Subramanian, Srikrishna. Council of Scientific & Industrial Research. Institute of Microbial Technology; India
Fil: Sharma, Deepak. Council of Scientific & Industrial Research. Institute of Microbial Technology; India
Fil: Adesh, Saini K.. Shoolini University; India
description Members of Burkholderia genus are gaining importance for their application in crop improvement by acting as plant growth-promoting rhizobacteria (PGPR), however, our knowledge about mechanisms of their plant growth promotion is limited. Herein, we aimed to isolate Burkholderia sp. from pea rhizosphere that confers plant growth promoting traits both in vitro and in vivo using pea seedlings as model. We have isolated a novel Burkholderia strain AU4i (B-AU4i) from pea rhizosphere that strongly promotes root and shoot growth in plantae. B-AU4i confers phosphate solubilization, indole-3-acetic acid production, N2 fixation, ammonia production, siderophore production, HCN production, and inhibits growth of pathogenic fungi both in vitro and in vivo experiments where we employed pea-seedlings as model system. Sequencing of B-AU4i genome using the Illumina-HiSeq 1000 technology reveals that it contains genes for the above-mentioned biofertilizer and biocontrol activities. The findings demonstrate the potential use of B-AU4i as plant growth promoter, which could be due to the presence of relevant genes in its genome. The current study improves our knowledge regarding the genes present in Burkholderia spp. that are involved in its plant growth promotion activities. B-AU4i strain can be used for improving agriculture productivity owing to its strong biofertilizer and biocontrol activity.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54023
Usha, Devi; Khatri, Indu; Lalit, Kumar; Devender, Singh; Aditi, Gupta; et al.; Genomic and Functional Characterization of a Novel Burkholderia sp. Strain AU4i from Pea Rhizosphere Conferring Plant Growth Promoting Activities; OMICS International; Advancements in Genetic Engineering; 4; 3; 9-2015; 1-8
2169-0111
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54023
identifier_str_mv Usha, Devi; Khatri, Indu; Lalit, Kumar; Devender, Singh; Aditi, Gupta; et al.; Genomic and Functional Characterization of a Novel Burkholderia sp. Strain AU4i from Pea Rhizosphere Conferring Plant Growth Promoting Activities; OMICS International; Advancements in Genetic Engineering; 4; 3; 9-2015; 1-8
2169-0111
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4/2169-0111.1000129
info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/genomic-and-functional-characterization-of-a-novel-burkholderia-sp-strainau4i-from-pea-rhizosphere-conferring-plant-growth-promoting-activities-2169-0111-1000129.php?aid=63316
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv OMICS International
publisher.none.fl_str_mv OMICS International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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