Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an ag...

Autores: García, Sabrina Soledad; Bernabeu, Pamela Romina; Vio, Santiago Adolfo; Cattelan, Natalia; Garcia, Julia Elena; Puente, Mariana Laura; María Lina, Galar; Prieto, Claudia Inés; Luna, María Flavia
Año de publicación: 2019
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Aims Characterization of barley-tissue–colonization efficiency (Hordeum vulgare L.) by Paraburkholderia tropica MTo-293 after seed inoculation was studied in two plant-growth systems: (1) in flasks with semisolid agar-containing sterile medium, as a suitable environment to study plant-bacteria interaction and also optimize molecular-biology techniques, and (2) in a potsubstrate system as an approach to understand their potential behavior as bioinput. Methods Culture-dependent techniques were implemented to quantify surface and endophytic bacterial populations in plant tissues. Culture-independent techniques were employed to detect and localize the inoculated bacteria in tissue samples along with evaluating the biofilm-forming capability by epifluorescent, confocallaser- scanning, and scanning-electron microscopy. Plant-growth parameters were measured to evaluate the effects of the inoculated acbteria on the development of the barley plants. Results Paraburkholderia tropica grew as a biofilm on both abiotic and biotic surfaces and efficiently colonized barley roots and stems in plants grown in flasks in presence of other microorganisms. The bacteria was localized on root surfaces, hairs, and central-cylinder areas. Paraburkholderia tropica also colonized the roots and stems in plants grown in the pot-substrate system. Although no endophytic root colonization occurred, the presence of the inoculated bacteria improved the aerial weight. A nested PCR detected P. tropica in the tissue samples. Conclusions Paraburkholderia tropica MTo-293 was characterized as an efficient biofilm-forming and barley- tissue–colonizing bacterium despite the presence of other microorganisms, but root endophytic colonization resulted dependent on the plant-growth system. Molecular-biology techniques were optimized, and also, its presence was correlated with plant-growth–promoting activity.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Garcia, Sabrina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina
Fil: Bernabeu, Pamela Romina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina
Fil: Vio, Santiago Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina
Fil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina
Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: María Lina, Galar. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina
Fil: Prieto, Claudia Inés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina
Fil: Luna, María Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI). Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-PBA)La Plata; Argentina
Fuente: Plant Soil (26 June 2019)
Materia: Barley
Cebada
Biofilm
Bioinput
Colonization
Plant-growth–promoting bacteria
Paraburkholderia
Nivel de accesibilidad: acceso restringido
Condiciones de uso
Repositorio
Institución: Instituto Nacional de Tecnología Agropecuaria
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spelling	Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinputGarcía, Sabrina SoledadBernabeu, Pamela RominaVio, Santiago AdolfoCattelan, NataliaGarcia, Julia ElenaPuente, Mariana LauraMaría Lina, GalarPrieto, Claudia InésLuna, María FlaviaBarleyCebadaBiofilmBioinputColonizationPlant-growth–promoting bacteriaParaburkholderiaAims Characterization of barley-tissue–colonization efficiency (Hordeum vulgare L.) by Paraburkholderia tropica MTo-293 after seed inoculation was studied in two plant-growth systems: (1) in flasks with semisolid agar-containing sterile medium, as a suitable environment to study plant-bacteria interaction and also optimize molecular-biology techniques, and (2) in a potsubstrate system as an approach to understand their potential behavior as bioinput. Methods Culture-dependent techniques were implemented to quantify surface and endophytic bacterial populations in plant tissues. Culture-independent techniques were employed to detect and localize the inoculated bacteria in tissue samples along with evaluating the biofilm-forming capability by epifluorescent, confocallaser- scanning, and scanning-electron microscopy. Plant-growth parameters were measured to evaluate the effects of the inoculated acbteria on the development of the barley plants. Results Paraburkholderia tropica grew as a biofilm on both abiotic and biotic surfaces and efficiently colonized barley roots and stems in plants grown in flasks in presence of other microorganisms. The bacteria was localized on root surfaces, hairs, and central-cylinder areas. Paraburkholderia tropica also colonized the roots and stems in plants grown in the pot-substrate system. Although no endophytic root colonization occurred, the presence of the inoculated bacteria improved the aerial weight. A nested PCR detected P. tropica in the tissue samples. Conclusions Paraburkholderia tropica MTo-293 was characterized as an efficient biofilm-forming and barley- tissue–colonizing bacterium despite the presence of other microorganisms, but root endophytic colonization resulted dependent on the plant-growth system. Molecular-biology techniques were optimized, and also, its presence was correlated with plant-growth–promoting activity.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Garcia, Sabrina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); ArgentinaFil: Bernabeu, Pamela Romina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); ArgentinaFil: Vio, Santiago Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); ArgentinaFil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); ArgentinaFil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: María Lina, Galar. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); ArgentinaFil: Prieto, Claudia Inés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); ArgentinaFil: Luna, María Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI). Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-PBA)La Plata; ArgentinaSpringer International Publishing2019-12-12T18:32:55Z2019-12-12T18:32:55Z2019-06-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007%2Fs11104-019-04174-yhttp://hdl.handle.net/20.500.12123/65020032-079X1573-5036https://doi.org/10.1007/s11104-019-04174-yPlant Soil (26 June 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:51Zoai:localhost:20.500.12123/6502instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:51.447INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv	Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput
title	Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput
spellingShingle	Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput García, Sabrina Soledad Barley Cebada Biofilm Bioinput Colonization Plant-growth–promoting bacteria Paraburkholderia
title_short	Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput
title_full	Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput
title_fullStr	Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput
title_full_unstemmed	Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput
title_sort	Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput
dc.creator.none.fl_str_mv	García, Sabrina Soledad Bernabeu, Pamela Romina Vio, Santiago Adolfo Cattelan, Natalia Garcia, Julia Elena Puente, Mariana Laura María Lina, Galar Prieto, Claudia Inés Luna, María Flavia
author	García, Sabrina Soledad
author_facet	García, Sabrina Soledad Bernabeu, Pamela Romina Vio, Santiago Adolfo Cattelan, Natalia Garcia, Julia Elena Puente, Mariana Laura María Lina, Galar Prieto, Claudia Inés Luna, María Flavia
author_role	author
author2	Bernabeu, Pamela Romina Vio, Santiago Adolfo Cattelan, Natalia Garcia, Julia Elena Puente, Mariana Laura María Lina, Galar Prieto, Claudia Inés Luna, María Flavia
author2_role	author author author author author author author author
dc.subject.none.fl_str_mv	Barley Cebada Biofilm Bioinput Colonization Plant-growth–promoting bacteria Paraburkholderia
topic	Barley Cebada Biofilm Bioinput Colonization Plant-growth–promoting bacteria Paraburkholderia
dc.description.none.fl_txt_mv	Aims Characterization of barley-tissue–colonization efficiency (Hordeum vulgare L.) by Paraburkholderia tropica MTo-293 after seed inoculation was studied in two plant-growth systems: (1) in flasks with semisolid agar-containing sterile medium, as a suitable environment to study plant-bacteria interaction and also optimize molecular-biology techniques, and (2) in a potsubstrate system as an approach to understand their potential behavior as bioinput. Methods Culture-dependent techniques were implemented to quantify surface and endophytic bacterial populations in plant tissues. Culture-independent techniques were employed to detect and localize the inoculated bacteria in tissue samples along with evaluating the biofilm-forming capability by epifluorescent, confocallaser- scanning, and scanning-electron microscopy. Plant-growth parameters were measured to evaluate the effects of the inoculated acbteria on the development of the barley plants. Results Paraburkholderia tropica grew as a biofilm on both abiotic and biotic surfaces and efficiently colonized barley roots and stems in plants grown in flasks in presence of other microorganisms. The bacteria was localized on root surfaces, hairs, and central-cylinder areas. Paraburkholderia tropica also colonized the roots and stems in plants grown in the pot-substrate system. Although no endophytic root colonization occurred, the presence of the inoculated bacteria improved the aerial weight. A nested PCR detected P. tropica in the tissue samples. Conclusions Paraburkholderia tropica MTo-293 was characterized as an efficient biofilm-forming and barley- tissue–colonizing bacterium despite the presence of other microorganisms, but root endophytic colonization resulted dependent on the plant-growth system. Molecular-biology techniques were optimized, and also, its presence was correlated with plant-growth–promoting activity. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Garcia, Sabrina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina Fil: Bernabeu, Pamela Romina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina Fil: Vio, Santiago Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina Fil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: María Lina, Galar. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina Fil: Prieto, Claudia Inés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina Fil: Luna, María Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI). Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-PBA)La Plata; Argentina
description	Aims Characterization of barley-tissue–colonization efficiency (Hordeum vulgare L.) by Paraburkholderia tropica MTo-293 after seed inoculation was studied in two plant-growth systems: (1) in flasks with semisolid agar-containing sterile medium, as a suitable environment to study plant-bacteria interaction and also optimize molecular-biology techniques, and (2) in a potsubstrate system as an approach to understand their potential behavior as bioinput. Methods Culture-dependent techniques were implemented to quantify surface and endophytic bacterial populations in plant tissues. Culture-independent techniques were employed to detect and localize the inoculated bacteria in tissue samples along with evaluating the biofilm-forming capability by epifluorescent, confocallaser- scanning, and scanning-electron microscopy. Plant-growth parameters were measured to evaluate the effects of the inoculated acbteria on the development of the barley plants. Results Paraburkholderia tropica grew as a biofilm on both abiotic and biotic surfaces and efficiently colonized barley roots and stems in plants grown in flasks in presence of other microorganisms. The bacteria was localized on root surfaces, hairs, and central-cylinder areas. Paraburkholderia tropica also colonized the roots and stems in plants grown in the pot-substrate system. Although no endophytic root colonization occurred, the presence of the inoculated bacteria improved the aerial weight. A nested PCR detected P. tropica in the tissue samples. Conclusions Paraburkholderia tropica MTo-293 was characterized as an efficient biofilm-forming and barley- tissue–colonizing bacterium despite the presence of other microorganisms, but root endophytic colonization resulted dependent on the plant-growth system. Molecular-biology techniques were optimized, and also, its presence was correlated with plant-growth–promoting activity.
publishDate	2019
dc.date.none.fl_str_mv	2019-12-12T18:32:55Z 2019-12-12T18:32:55Z 2019-06-26
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://link.springer.com/article/10.1007%2Fs11104-019-04174-y http://hdl.handle.net/20.500.12123/6502 0032-079X 1573-5036 https://doi.org/10.1007/s11104-019-04174-y
url	https://link.springer.com/article/10.1007%2Fs11104-019-04174-y http://hdl.handle.net/20.500.12123/6502 https://doi.org/10.1007/s11104-019-04174-y
identifier_str_mv	0032-079X 1573-5036
dc.language.none.fl_str_mv	eng
language	eng
dc.rights.none.fl_str_mv	info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv	restrictedAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Springer International Publishing
publisher.none.fl_str_mv	Springer International Publishing
dc.source.none.fl_str_mv	Plant Soil (26 June 2019) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str	INTA Digital (INTA)
collection	INTA Digital (INTA)
instname_str	Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv	INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv	tripaldi.nicolas@inta.gob.ar
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Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an ag...

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