Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn

Autores
Buthet, Lara Romina; Maciel, María Eugenia; Quintans, Leandro; Rodríguez De Castro, Carmen; Costantini, Martin Hernán; Fanelli, Silvia Laura; Castro, Jose Alberto; Castro, Gerardo Daniel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
After alcohol exposure through a standard Lieber and De Carli diet for 28 days, a severe atrophy in the rat uteirne horn was observed, accompanied by significant alterations in its epithelial cells. Microsomal pathway of acetaldehyde production was slightly increased. Hydroxyl radicals were detected in the cytosolic fraction, and this was attributed to participation of xanthine oxidoreductase. They were also observed in the microsomal fraction in the presence of NADPH generating system. No generation of 1-hydroxyethyl was evidenced. The t-butylhydroperoxide-induced chemiluminescence analysis of uterine horn homogenates revealed a significant increase in the chemiluminiscence emission due to ethanol exposure. In the animals repeatedly exposed to alcohol, sulfhydryl content from uterine horn proteins was decreased, but no significant changes were observed in the protein carbonyl content from the same samples. Minor but significant decreasing changes were observed in the GSH content accompanied by a tendency to decrease in the GSH/GSSG ratio. A highly significant finding was the diminished activity content of glutathione peroxidase. Results suggest that acetaldehyde accumulation plus the oxidative stress may play an additional effect to the alcohol-promoted hormonal changes in the uterus reported by others after chronic exposure to alcohol.
Instituto de Limnología "Dr. Raúl A. Ringuelet"
Materia
Biología
Uterus
Ethanol
Acetaldehyde
Oxidative stress
Reproductive toxicity
Alcohol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/104493
Acceder
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network_name_str SEDICI (UNLP)
spelling Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine hornButhet, Lara RominaMaciel, María EugeniaQuintans, LeandroRodríguez De Castro, CarmenCostantini, Martin HernánFanelli, Silvia LauraCastro, Jose AlbertoCastro, Gerardo DanielBiologíaUterusEthanolAcetaldehydeOxidative stressReproductive toxicityAlcoholAfter alcohol exposure through a standard Lieber and De Carli diet for 28 days, a severe atrophy in the rat uteirne horn was observed, accompanied by significant alterations in its epithelial cells. Microsomal pathway of acetaldehyde production was slightly increased. Hydroxyl radicals were detected in the cytosolic fraction, and this was attributed to participation of xanthine oxidoreductase. They were also observed in the microsomal fraction in the presence of NADPH generating system. No generation of 1-hydroxyethyl was evidenced. The t-butylhydroperoxide-induced chemiluminescence analysis of uterine horn homogenates revealed a significant increase in the chemiluminiscence emission due to ethanol exposure. In the animals repeatedly exposed to alcohol, sulfhydryl content from uterine horn proteins was decreased, but no significant changes were observed in the protein carbonyl content from the same samples. Minor but significant decreasing changes were observed in the GSH content accompanied by a tendency to decrease in the GSH/GSSG ratio. A highly significant finding was the diminished activity content of glutathione peroxidase. Results suggest that acetaldehyde accumulation plus the oxidative stress may play an additional effect to the alcohol-promoted hormonal changes in the uterus reported by others after chronic exposure to alcohol.Instituto de Limnología "Dr. Raúl A. Ringuelet"2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/104493enginfo:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/76405info:eu-repo/semantics/altIdentifier/issn/1687-8205info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/161496info:eu-repo/semantics/altIdentifier/hdl/11336/76405info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:14:39Zoai:sedici.unlp.edu.ar:10915/104493Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:14:39.307SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
title Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
spellingShingle Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
Buthet, Lara Romina
Biología
Uterus
Ethanol
Acetaldehyde
Oxidative stress
Reproductive toxicity
Alcohol
title_short Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
title_full Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
title_fullStr Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
title_full_unstemmed Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
title_sort Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
dc.creator.none.fl_str_mv Buthet, Lara Romina
Maciel, María Eugenia
Quintans, Leandro
Rodríguez De Castro, Carmen
Costantini, Martin Hernán
Fanelli, Silvia Laura
Castro, Jose Alberto
Castro, Gerardo Daniel
author Buthet, Lara Romina
author_facet Buthet, Lara Romina
Maciel, María Eugenia
Quintans, Leandro
Rodríguez De Castro, Carmen
Costantini, Martin Hernán
Fanelli, Silvia Laura
Castro, Jose Alberto
Castro, Gerardo Daniel
author_role author
author2 Maciel, María Eugenia
Quintans, Leandro
Rodríguez De Castro, Carmen
Costantini, Martin Hernán
Fanelli, Silvia Laura
Castro, Jose Alberto
Castro, Gerardo Daniel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Uterus
Ethanol
Acetaldehyde
Oxidative stress
Reproductive toxicity
Alcohol
topic Biología
Uterus
Ethanol
Acetaldehyde
Oxidative stress
Reproductive toxicity
Alcohol
dc.description.none.fl_txt_mv After alcohol exposure through a standard Lieber and De Carli diet for 28 days, a severe atrophy in the rat uteirne horn was observed, accompanied by significant alterations in its epithelial cells. Microsomal pathway of acetaldehyde production was slightly increased. Hydroxyl radicals were detected in the cytosolic fraction, and this was attributed to participation of xanthine oxidoreductase. They were also observed in the microsomal fraction in the presence of NADPH generating system. No generation of 1-hydroxyethyl was evidenced. The t-butylhydroperoxide-induced chemiluminescence analysis of uterine horn homogenates revealed a significant increase in the chemiluminiscence emission due to ethanol exposure. In the animals repeatedly exposed to alcohol, sulfhydryl content from uterine horn proteins was decreased, but no significant changes were observed in the protein carbonyl content from the same samples. Minor but significant decreasing changes were observed in the GSH content accompanied by a tendency to decrease in the GSH/GSSG ratio. A highly significant finding was the diminished activity content of glutathione peroxidase. Results suggest that acetaldehyde accumulation plus the oxidative stress may play an additional effect to the alcohol-promoted hormonal changes in the uterus reported by others after chronic exposure to alcohol.
Instituto de Limnología "Dr. Raúl A. Ringuelet"
description After alcohol exposure through a standard Lieber and De Carli diet for 28 days, a severe atrophy in the rat uteirne horn was observed, accompanied by significant alterations in its epithelial cells. Microsomal pathway of acetaldehyde production was slightly increased. Hydroxyl radicals were detected in the cytosolic fraction, and this was attributed to participation of xanthine oxidoreductase. They were also observed in the microsomal fraction in the presence of NADPH generating system. No generation of 1-hydroxyethyl was evidenced. The t-butylhydroperoxide-induced chemiluminescence analysis of uterine horn homogenates revealed a significant increase in the chemiluminiscence emission due to ethanol exposure. In the animals repeatedly exposed to alcohol, sulfhydryl content from uterine horn proteins was decreased, but no significant changes were observed in the protein carbonyl content from the same samples. Minor but significant decreasing changes were observed in the GSH content accompanied by a tendency to decrease in the GSH/GSSG ratio. A highly significant finding was the diminished activity content of glutathione peroxidase. Results suggest that acetaldehyde accumulation plus the oxidative stress may play an additional effect to the alcohol-promoted hormonal changes in the uterus reported by others after chronic exposure to alcohol.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/104493
url http://sedici.unlp.edu.ar/handle/10915/104493
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/76405
info:eu-repo/semantics/altIdentifier/issn/1687-8205
info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/161496
info:eu-repo/semantics/altIdentifier/hdl/11336/76405
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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