Effect of galacto-oligosaccharides: maltodextrin matrices on the recovery of Lactobacillus plantarum after spray-drying

Autores
Sosa, Natalia; Gerbino, Oscar Esteban; Golowczyc, Marina Alejandra; Schebor, Carolina; Gómez-Zavaglia, Andrea; Tymczyszyn, Emma Elizabeth
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work maltodextrins were added to commercial galacto-oligosaccharides (GOS) in a 1:1 ratio and their thermophysical characteristics were analyzed. GOS:MD solutions were then used as matrices during spray-drying of Lactobacillus plantarum CIDCA 83114. The obtained powders were equilibrated at different relative humidities (RH) and stored at 5 and 20°C for 12 weeks, or at 30°C for 6 weeks. The Tgs of GOS:MD matrices were about 20-30°C higher than those of GOS at RH within 11 and 52%. A linear relation between the spin-spin relaxation time (T2) and T-Tg parameter was observed for GOS:MD matrices equilibrated at 11, 22, 33, and 44% RH at 5, 20, and 30°C. Spray-drying of L. plantarum CIDCA 83114 in GOS:MD matrices allowed the recovery of 93% microorganisms. In contrast, only 64% microorganisms were recovered when no GOS were included in the dehydration medium. Survival of L. plantarum CIDCA 83114 during storage showed the best performance for bacteria stored at 5°C. In a further step, the slopes of the linear regressions provided information about the rate of microbial inactivation for each storage condition (k values). This information can be useful to calculate the shelf-life of spray-dried starters stored at different temperatures and RH. Using GOS:MD matrices as a dehydration medium enhanced the recovery of L. plantarum CIDCA 83114 after spray-drying. This strategy allowed for the first time the spray-drying stabilization of a potentially probiotic strain in the presence of GOS.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Materia
Biología
Galacto-oligosaccharides
Glass transition temperature
Lactic acid bacteria
Maltodextrin
Spray-drying
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/86384

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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Effect of galacto-oligosaccharides: maltodextrin matrices on the recovery of Lactobacillus plantarum after spray-dryingSosa, NataliaGerbino, Oscar EstebanGolowczyc, Marina AlejandraSchebor, CarolinaGómez-Zavaglia, AndreaTymczyszyn, Emma ElizabethBiologíaGalacto-oligosaccharidesGlass transition temperatureLactic acid bacteriaMaltodextrinSpray-dryingIn this work maltodextrins were added to commercial galacto-oligosaccharides (GOS) in a 1:1 ratio and their thermophysical characteristics were analyzed. GOS:MD solutions were then used as matrices during spray-drying of Lactobacillus plantarum CIDCA 83114. The obtained powders were equilibrated at different relative humidities (RH) and stored at 5 and 20°C for 12 weeks, or at 30°C for 6 weeks. The Tgs of GOS:MD matrices were about 20-30°C higher than those of GOS at RH within 11 and 52%. A linear relation between the spin-spin relaxation time (T2) and T-Tg parameter was observed for GOS:MD matrices equilibrated at 11, 22, 33, and 44% RH at 5, 20, and 30°C. Spray-drying of L. plantarum CIDCA 83114 in GOS:MD matrices allowed the recovery of 93% microorganisms. In contrast, only 64% microorganisms were recovered when no GOS were included in the dehydration medium. Survival of L. plantarum CIDCA 83114 during storage showed the best performance for bacteria stored at 5°C. In a further step, the slopes of the linear regressions provided information about the rate of microbial inactivation for each storage condition (k values). This information can be useful to calculate the shelf-life of spray-dried starters stored at different temperatures and RH. Using GOS:MD matrices as a dehydration medium enhanced the recovery of L. plantarum CIDCA 83114 after spray-drying. This strategy allowed for the first time the spray-drying stabilization of a potentially probiotic strain in the presence of GOS.Centro de Investigación y Desarrollo en Criotecnología de Alimentos2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/86384enginfo:eu-repo/semantics/altIdentifier/issn/1664-302Xinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2016.00584info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:08:45Zoai:sedici.unlp.edu.ar:10915/86384Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:08:45.851SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Effect of galacto-oligosaccharides: maltodextrin matrices on the recovery of Lactobacillus plantarum after spray-drying
title Effect of galacto-oligosaccharides: maltodextrin matrices on the recovery of Lactobacillus plantarum after spray-drying
spellingShingle Effect of galacto-oligosaccharides: maltodextrin matrices on the recovery of Lactobacillus plantarum after spray-drying
Sosa, Natalia
Biología
Galacto-oligosaccharides
Glass transition temperature
Lactic acid bacteria
Maltodextrin
Spray-drying
title_short Effect of galacto-oligosaccharides: maltodextrin matrices on the recovery of Lactobacillus plantarum after spray-drying
title_full Effect of galacto-oligosaccharides: maltodextrin matrices on the recovery of Lactobacillus plantarum after spray-drying
title_fullStr Effect of galacto-oligosaccharides: maltodextrin matrices on the recovery of Lactobacillus plantarum after spray-drying
title_full_unstemmed Effect of galacto-oligosaccharides: maltodextrin matrices on the recovery of Lactobacillus plantarum after spray-drying
title_sort Effect of galacto-oligosaccharides: maltodextrin matrices on the recovery of Lactobacillus plantarum after spray-drying
dc.creator.none.fl_str_mv Sosa, Natalia
Gerbino, Oscar Esteban
Golowczyc, Marina Alejandra
Schebor, Carolina
Gómez-Zavaglia, Andrea
Tymczyszyn, Emma Elizabeth
author Sosa, Natalia
author_facet Sosa, Natalia
Gerbino, Oscar Esteban
Golowczyc, Marina Alejandra
Schebor, Carolina
Gómez-Zavaglia, Andrea
Tymczyszyn, Emma Elizabeth
author_role author
author2 Gerbino, Oscar Esteban
Golowczyc, Marina Alejandra
Schebor, Carolina
Gómez-Zavaglia, Andrea
Tymczyszyn, Emma Elizabeth
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Galacto-oligosaccharides
Glass transition temperature
Lactic acid bacteria
Maltodextrin
Spray-drying
topic Biología
Galacto-oligosaccharides
Glass transition temperature
Lactic acid bacteria
Maltodextrin
Spray-drying
dc.description.none.fl_txt_mv In this work maltodextrins were added to commercial galacto-oligosaccharides (GOS) in a 1:1 ratio and their thermophysical characteristics were analyzed. GOS:MD solutions were then used as matrices during spray-drying of Lactobacillus plantarum CIDCA 83114. The obtained powders were equilibrated at different relative humidities (RH) and stored at 5 and 20°C for 12 weeks, or at 30°C for 6 weeks. The Tgs of GOS:MD matrices were about 20-30°C higher than those of GOS at RH within 11 and 52%. A linear relation between the spin-spin relaxation time (T2) and T-Tg parameter was observed for GOS:MD matrices equilibrated at 11, 22, 33, and 44% RH at 5, 20, and 30°C. Spray-drying of L. plantarum CIDCA 83114 in GOS:MD matrices allowed the recovery of 93% microorganisms. In contrast, only 64% microorganisms were recovered when no GOS were included in the dehydration medium. Survival of L. plantarum CIDCA 83114 during storage showed the best performance for bacteria stored at 5°C. In a further step, the slopes of the linear regressions provided information about the rate of microbial inactivation for each storage condition (k values). This information can be useful to calculate the shelf-life of spray-dried starters stored at different temperatures and RH. Using GOS:MD matrices as a dehydration medium enhanced the recovery of L. plantarum CIDCA 83114 after spray-drying. This strategy allowed for the first time the spray-drying stabilization of a potentially probiotic strain in the presence of GOS.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
description In this work maltodextrins were added to commercial galacto-oligosaccharides (GOS) in a 1:1 ratio and their thermophysical characteristics were analyzed. GOS:MD solutions were then used as matrices during spray-drying of Lactobacillus plantarum CIDCA 83114. The obtained powders were equilibrated at different relative humidities (RH) and stored at 5 and 20°C for 12 weeks, or at 30°C for 6 weeks. The Tgs of GOS:MD matrices were about 20-30°C higher than those of GOS at RH within 11 and 52%. A linear relation between the spin-spin relaxation time (T2) and T-Tg parameter was observed for GOS:MD matrices equilibrated at 11, 22, 33, and 44% RH at 5, 20, and 30°C. Spray-drying of L. plantarum CIDCA 83114 in GOS:MD matrices allowed the recovery of 93% microorganisms. In contrast, only 64% microorganisms were recovered when no GOS were included in the dehydration medium. Survival of L. plantarum CIDCA 83114 during storage showed the best performance for bacteria stored at 5°C. In a further step, the slopes of the linear regressions provided information about the rate of microbial inactivation for each storage condition (k values). This information can be useful to calculate the shelf-life of spray-dried starters stored at different temperatures and RH. Using GOS:MD matrices as a dehydration medium enhanced the recovery of L. plantarum CIDCA 83114 after spray-drying. This strategy allowed for the first time the spray-drying stabilization of a potentially probiotic strain in the presence of GOS.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/86384
url http://sedici.unlp.edu.ar/handle/10915/86384
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1664-302X
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2016.00584
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
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institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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