Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation

Autores
Fantinelli, Juliana Catalina; Cuéllar Álvarez, Liceth N.; González Arbeláez, Luisa Fernanda; Ciocci Pardo, Alejandro; Galeano García, Paula; Schinella, Guillermo Raúl; Mosca, Susana María
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Our aim was to examine the effects of aqueous extracts of fermented (CFE) and non-fermented (CNFE) ¨copoazú¨ (fruit of Theobroma grandiflorum) seeds against ischemia-reperfusion injury. Isolated rat hearts were submitted to 30 min of global ischemia (GI) and 60 min of reperfusion (R). Other hearts received CFE or CNFE in absence or in presence of L-NAME (a nitric oxide synthase inhibitor). Infarct size (IS) and postischemic myocardial function (PMF) were measured. Lipid peroxidation, reduced glutathione (GSH) and the expression of phosphorylated forms of eNOS, Akt, GSK-3b and PKCe were assessed. The response of isolated mitochondria to Ca2+ (MR) was also determined. CFE but not CNFE decreased IS, increased PMF and the expression of P-eNOS, P-Akt, P-GSK-3b and P-PKCe, partially preserved GSH and improved MR. These effects were lost in presence of L-NAME. These data demonstrate that the acute treatment with CFE protects the heart against ischemiareperfusion damage through NOS-dependent mechanisms.
Facultad de Ciencias Médicas (FCM)
Materia
Ciencias Médicas
Copoazú
Fermentation
Ischemia-reperfusion
NOS
Mitochondria
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/80930
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/80930
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activationFantinelli, Juliana CatalinaCuéllar Álvarez, Liceth N.González Arbeláez, Luisa FernandaCiocci Pardo, AlejandroGaleano García, PaulaSchinella, Guillermo RaúlMosca, Susana MaríaCiencias MédicasCopoazúFermentationIschemia-reperfusionNOSMitochondriaOur aim was to examine the effects of aqueous extracts of fermented (CFE) and non-fermented (CNFE) ¨copoazú¨ (fruit of Theobroma grandiflorum) seeds against ischemia-reperfusion injury. Isolated rat hearts were submitted to 30 min of global ischemia (GI) and 60 min of reperfusion (R). Other hearts received CFE or CNFE in absence or in presence of L-NAME (a nitric oxide synthase inhibitor). Infarct size (IS) and postischemic myocardial function (PMF) were measured. Lipid peroxidation, reduced glutathione (GSH) and the expression of phosphorylated forms of eNOS, Akt, GSK-3b and PKCe were assessed. The response of isolated mitochondria to Ca2+ (MR) was also determined. CFE but not CNFE decreased IS, increased PMF and the expression of P-eNOS, P-Akt, P-GSK-3b and P-PKCe, partially preserved GSH and improved MR. These effects were lost in presence of L-NAME. These data demonstrate that the acute treatment with CFE protects the heart against ischemiareperfusion damage through NOS-dependent mechanisms.Facultad de Ciencias Médicas (FCM)2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf470-477http://sedici.unlp.edu.ar/handle/10915/80930enginfo:eu-repo/semantics/altIdentifier/issn/1756-4646info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jff.2017.05.010info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:14:57Zoai:sedici.unlp.edu.ar:10915/80930Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:14:57.865SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
spellingShingle Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
Fantinelli, Juliana Catalina
Ciencias Médicas
Copoazú
Fermentation
Ischemia-reperfusion
NOS
Mitochondria
title_short Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title_full Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title_fullStr Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title_full_unstemmed Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title_sort Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
dc.creator.none.fl_str_mv Fantinelli, Juliana Catalina
Cuéllar Álvarez, Liceth N.
González Arbeláez, Luisa Fernanda
Ciocci Pardo, Alejandro
Galeano García, Paula
Schinella, Guillermo Raúl
Mosca, Susana María
author Fantinelli, Juliana Catalina
author_facet Fantinelli, Juliana Catalina
Cuéllar Álvarez, Liceth N.
González Arbeláez, Luisa Fernanda
Ciocci Pardo, Alejandro
Galeano García, Paula
Schinella, Guillermo Raúl
Mosca, Susana María
author_role author
author2 Cuéllar Álvarez, Liceth N.
González Arbeláez, Luisa Fernanda
Ciocci Pardo, Alejandro
Galeano García, Paula
Schinella, Guillermo Raúl
Mosca, Susana María
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
Copoazú
Fermentation
Ischemia-reperfusion
NOS
Mitochondria
topic Ciencias Médicas
Copoazú
Fermentation
Ischemia-reperfusion
NOS
Mitochondria
dc.description.none.fl_txt_mv Our aim was to examine the effects of aqueous extracts of fermented (CFE) and non-fermented (CNFE) ¨copoazú¨ (fruit of Theobroma grandiflorum) seeds against ischemia-reperfusion injury. Isolated rat hearts were submitted to 30 min of global ischemia (GI) and 60 min of reperfusion (R). Other hearts received CFE or CNFE in absence or in presence of L-NAME (a nitric oxide synthase inhibitor). Infarct size (IS) and postischemic myocardial function (PMF) were measured. Lipid peroxidation, reduced glutathione (GSH) and the expression of phosphorylated forms of eNOS, Akt, GSK-3b and PKCe were assessed. The response of isolated mitochondria to Ca2+ (MR) was also determined. CFE but not CNFE decreased IS, increased PMF and the expression of P-eNOS, P-Akt, P-GSK-3b and P-PKCe, partially preserved GSH and improved MR. These effects were lost in presence of L-NAME. These data demonstrate that the acute treatment with CFE protects the heart against ischemiareperfusion damage through NOS-dependent mechanisms.
Facultad de Ciencias Médicas (FCM)
description Our aim was to examine the effects of aqueous extracts of fermented (CFE) and non-fermented (CNFE) ¨copoazú¨ (fruit of Theobroma grandiflorum) seeds against ischemia-reperfusion injury. Isolated rat hearts were submitted to 30 min of global ischemia (GI) and 60 min of reperfusion (R). Other hearts received CFE or CNFE in absence or in presence of L-NAME (a nitric oxide synthase inhibitor). Infarct size (IS) and postischemic myocardial function (PMF) were measured. Lipid peroxidation, reduced glutathione (GSH) and the expression of phosphorylated forms of eNOS, Akt, GSK-3b and PKCe were assessed. The response of isolated mitochondria to Ca2+ (MR) was also determined. CFE but not CNFE decreased IS, increased PMF and the expression of P-eNOS, P-Akt, P-GSK-3b and P-PKCe, partially preserved GSH and improved MR. These effects were lost in presence of L-NAME. These data demonstrate that the acute treatment with CFE protects the heart against ischemiareperfusion damage through NOS-dependent mechanisms.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/80930
url http://sedici.unlp.edu.ar/handle/10915/80930
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1756-4646
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jff.2017.05.010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
470-477
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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