Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation

Autores
Fantinelli, Juliana Catalina; Cuéllar Álvarez, Liceth N.; González Arbeláez, Luisa Fernanda; Ciocci Pardo, Alejandro; Galeano García, Paula L.; Schinella, Guillermo Raúl; Mosca, Susana Maria
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Our aim was to examine the effects of aqueous extracts of fermented (CFE) and non-fermented (CNFE) ¨copoazú¨ (fruit of Theobroma grandiflorum) seeds against ischemia-reperfusion injury. Isolated rat hearts were submitted to 30 min of global ischemia (GI) and 60 min of reperfusion (R). Other hearts received CFE or CNFE in absence or in presence of L-NAME (a nitric oxide synthase inhibitor). Infarct size (IS) and post-ischemic myocardial function (PMF) were measured. Lipid peroxidation, reduced glutathione (GSH) and the expression of phosphorylated forms of eNOS, Akt, GSK-3β and PKCε were assessed. The response of isolated mitochondria to Ca2+ (MR) was also determined. CFE but not CNFE decreased IS, increased PMF and the expression of P-eNOS, P-Akt, P-GSK-3β and P-PKCε, partially preserved GSH and improved MR. These effects were lost in presence of L-NAME. These data demonstrate that the acute treatment with CFE protects the heart against ischemia-reperfusion damage through NOS-dependent mechanisms.
Fil: Fantinelli, Juliana Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Cuéllar Álvarez, Liceth N.. Universidad de la Amazonia; Colombia
Fil: González Arbeláez, Luisa Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Ciocci Pardo, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Galeano García, Paula L.. Universidad de la Amazonia; Colombia
Fil: Schinella, Guillermo Raúl. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Mosca, Susana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Materia
CopoazÚ
Fermentation
Ischemia-Reperfusion
Mitochondria
Nos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54386
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54386
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activationFantinelli, Juliana CatalinaCuéllar Álvarez, Liceth N.González Arbeláez, Luisa FernandaCiocci Pardo, AlejandroGaleano García, Paula L.Schinella, Guillermo RaúlMosca, Susana MariaCopoazÚFermentationIschemia-ReperfusionMitochondriaNoshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Our aim was to examine the effects of aqueous extracts of fermented (CFE) and non-fermented (CNFE) ¨copoazú¨ (fruit of Theobroma grandiflorum) seeds against ischemia-reperfusion injury. Isolated rat hearts were submitted to 30 min of global ischemia (GI) and 60 min of reperfusion (R). Other hearts received CFE or CNFE in absence or in presence of L-NAME (a nitric oxide synthase inhibitor). Infarct size (IS) and post-ischemic myocardial function (PMF) were measured. Lipid peroxidation, reduced glutathione (GSH) and the expression of phosphorylated forms of eNOS, Akt, GSK-3β and PKCε were assessed. The response of isolated mitochondria to Ca2+ (MR) was also determined. CFE but not CNFE decreased IS, increased PMF and the expression of P-eNOS, P-Akt, P-GSK-3β and P-PKCε, partially preserved GSH and improved MR. These effects were lost in presence of L-NAME. These data demonstrate that the acute treatment with CFE protects the heart against ischemia-reperfusion damage through NOS-dependent mechanisms.Fil: Fantinelli, Juliana Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; ArgentinaFil: Cuéllar Álvarez, Liceth N.. Universidad de la Amazonia; ColombiaFil: González Arbeláez, Luisa Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; ArgentinaFil: Ciocci Pardo, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; ArgentinaFil: Galeano García, Paula L.. Universidad de la Amazonia; ColombiaFil: Schinella, Guillermo Raúl. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Mosca, Susana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; ArgentinaElsevier Ltd2017-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54386Fantinelli, Juliana Catalina; Cuéllar Álvarez, Liceth N.; González Arbeláez, Luisa Fernanda; Ciocci Pardo, Alejandro; Galeano García, Paula L.; et al.; Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation; Elsevier Ltd; Journal of Functional Foods; 34; 7-2017; 470-4771756-4646CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jff.2017.05.010info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1756464617302694info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:03Zoai:ri.conicet.gov.ar:11336/54386instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:03.52CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
spellingShingle Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
Fantinelli, Juliana Catalina
CopoazÚ
Fermentation
Ischemia-Reperfusion
Mitochondria
Nos
title_short Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title_full Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title_fullStr Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title_full_unstemmed Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title_sort Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
dc.creator.none.fl_str_mv Fantinelli, Juliana Catalina
Cuéllar Álvarez, Liceth N.
González Arbeláez, Luisa Fernanda
Ciocci Pardo, Alejandro
Galeano García, Paula L.
Schinella, Guillermo Raúl
Mosca, Susana Maria
author Fantinelli, Juliana Catalina
author_facet Fantinelli, Juliana Catalina
Cuéllar Álvarez, Liceth N.
González Arbeláez, Luisa Fernanda
Ciocci Pardo, Alejandro
Galeano García, Paula L.
Schinella, Guillermo Raúl
Mosca, Susana Maria
author_role author
author2 Cuéllar Álvarez, Liceth N.
González Arbeláez, Luisa Fernanda
Ciocci Pardo, Alejandro
Galeano García, Paula L.
Schinella, Guillermo Raúl
Mosca, Susana Maria
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CopoazÚ
Fermentation
Ischemia-Reperfusion
Mitochondria
Nos
topic CopoazÚ
Fermentation
Ischemia-Reperfusion
Mitochondria
Nos
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Our aim was to examine the effects of aqueous extracts of fermented (CFE) and non-fermented (CNFE) ¨copoazú¨ (fruit of Theobroma grandiflorum) seeds against ischemia-reperfusion injury. Isolated rat hearts were submitted to 30 min of global ischemia (GI) and 60 min of reperfusion (R). Other hearts received CFE or CNFE in absence or in presence of L-NAME (a nitric oxide synthase inhibitor). Infarct size (IS) and post-ischemic myocardial function (PMF) were measured. Lipid peroxidation, reduced glutathione (GSH) and the expression of phosphorylated forms of eNOS, Akt, GSK-3β and PKCε were assessed. The response of isolated mitochondria to Ca2+ (MR) was also determined. CFE but not CNFE decreased IS, increased PMF and the expression of P-eNOS, P-Akt, P-GSK-3β and P-PKCε, partially preserved GSH and improved MR. These effects were lost in presence of L-NAME. These data demonstrate that the acute treatment with CFE protects the heart against ischemia-reperfusion damage through NOS-dependent mechanisms.
Fil: Fantinelli, Juliana Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Cuéllar Álvarez, Liceth N.. Universidad de la Amazonia; Colombia
Fil: González Arbeláez, Luisa Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Ciocci Pardo, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Galeano García, Paula L.. Universidad de la Amazonia; Colombia
Fil: Schinella, Guillermo Raúl. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Mosca, Susana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
description Our aim was to examine the effects of aqueous extracts of fermented (CFE) and non-fermented (CNFE) ¨copoazú¨ (fruit of Theobroma grandiflorum) seeds against ischemia-reperfusion injury. Isolated rat hearts were submitted to 30 min of global ischemia (GI) and 60 min of reperfusion (R). Other hearts received CFE or CNFE in absence or in presence of L-NAME (a nitric oxide synthase inhibitor). Infarct size (IS) and post-ischemic myocardial function (PMF) were measured. Lipid peroxidation, reduced glutathione (GSH) and the expression of phosphorylated forms of eNOS, Akt, GSK-3β and PKCε were assessed. The response of isolated mitochondria to Ca2+ (MR) was also determined. CFE but not CNFE decreased IS, increased PMF and the expression of P-eNOS, P-Akt, P-GSK-3β and P-PKCε, partially preserved GSH and improved MR. These effects were lost in presence of L-NAME. These data demonstrate that the acute treatment with CFE protects the heart against ischemia-reperfusion damage through NOS-dependent mechanisms.
publishDate 2017
dc.date.none.fl_str_mv 2017-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54386
Fantinelli, Juliana Catalina; Cuéllar Álvarez, Liceth N.; González Arbeláez, Luisa Fernanda; Ciocci Pardo, Alejandro; Galeano García, Paula L.; et al.; Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation; Elsevier Ltd; Journal of Functional Foods; 34; 7-2017; 470-477
1756-4646
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54386
identifier_str_mv Fantinelli, Juliana Catalina; Cuéllar Álvarez, Liceth N.; González Arbeláez, Luisa Fernanda; Ciocci Pardo, Alejandro; Galeano García, Paula L.; et al.; Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation; Elsevier Ltd; Journal of Functional Foods; 34; 7-2017; 470-477
1756-4646
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jff.2017.05.010
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1756464617302694
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd
publisher.none.fl_str_mv Elsevier Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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