The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol

Autores
Assad, Sabrina; Fragomeno, Melisa; Rumbo, Martín; Minnaard, Jessica; Pérez, Pablo Fernando
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bifidobacteria are health promoting microorganisms that can interact with drugs in the intestinal tract thus modifying the activation of immune cells and host homeostasis. To study the effect of acenocoumarol on the interaction between bifidobacteria and THP-1 cells or intestinal epithelial cells. Bifidobacterium bifidum CIDCA 5310 and B. adolescentis CIDCA 5317 were grown in MRS broth in anaerobic conditions. The experiments were done in the presence of acenocoumarol. FITC-labeled bacteria were incubated with THP1 cells differentiated with PMA (THP1-PMA), and phagocytosis and intracellular localization of bacteria were assessed by flow cytometry and confocal laser microscopy, respectively. Also, expression of macrophage surface molecules in THP1 and THP1-PMA cells was assessed by flow cytometry. By using the Caco-2 ccl20:luc reporter system innate immune response was analysed by determining luciferase expression induced by flagellin. Acenocoumarol decreased phagocytosis of bifidobacteria but intracellular routing was not modified. Also, anticoagulant reduced the expression of HLA-DR and CD64 induced by bifidobacteria in both THP1-PMA and THP1 cells. Instead, the decrease in the expression of CD206 and CD23 was dependent on differentiation status of THP1 cells. Acenocoumarol increased CCL20 expression and it was partially abrogated by strain CIDCA 5310. Presence of acenocoumarol modifies the interaction between bifidobacteria and eukaryotic cells thus suggesting that the expected modulation of immune response by probiotic foods can be different in persons under anticoagulant treatment.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Instituto de Estudios Inmunológicos y Fisiopatológicos
Materia
Química
Bifidobacterium
Acenocoumarol
THP-1 cells
Caco-2 ccl20:luc reporter system
Immune response
Anticoagulant
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/166688
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/166688
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarolAssad, SabrinaFragomeno, MelisaRumbo, MartínMinnaard, JessicaPérez, Pablo FernandoQuímicaBifidobacteriumAcenocoumarolTHP-1 cellsCaco-2 ccl20:luc reporter systemImmune responseAnticoagulantBifidobacteria are health promoting microorganisms that can interact with drugs in the intestinal tract thus modifying the activation of immune cells and host homeostasis. To study the effect of acenocoumarol on the interaction between bifidobacteria and THP-1 cells or intestinal epithelial cells. Bifidobacterium bifidum CIDCA 5310 and B. adolescentis CIDCA 5317 were grown in MRS broth in anaerobic conditions. The experiments were done in the presence of acenocoumarol. FITC-labeled bacteria were incubated with THP1 cells differentiated with PMA (THP1-PMA), and phagocytosis and intracellular localization of bacteria were assessed by flow cytometry and confocal laser microscopy, respectively. Also, expression of macrophage surface molecules in THP1 and THP1-PMA cells was assessed by flow cytometry. By using the Caco-2 ccl20:luc reporter system innate immune response was analysed by determining luciferase expression induced by flagellin. Acenocoumarol decreased phagocytosis of bifidobacteria but intracellular routing was not modified. Also, anticoagulant reduced the expression of HLA-DR and CD64 induced by bifidobacteria in both THP1-PMA and THP1 cells. Instead, the decrease in the expression of CD206 and CD23 was dependent on differentiation status of THP1 cells. Acenocoumarol increased CCL20 expression and it was partially abrogated by strain CIDCA 5310. Presence of acenocoumarol modifies the interaction between bifidobacteria and eukaryotic cells thus suggesting that the expected modulation of immune response by probiotic foods can be different in persons under anticoagulant treatment.Centro de Investigación y Desarrollo en Criotecnología de AlimentosInstituto de Estudios Inmunológicos y Fisiopatológicos2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/166688enginfo:eu-repo/semantics/altIdentifier/issn/2212-4292info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fbio.2023.102473info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-17T10:27:02Zoai:sedici.unlp.edu.ar:10915/166688Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-17 10:27:02.635SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
title The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
spellingShingle The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
Assad, Sabrina
Química
Bifidobacterium
Acenocoumarol
THP-1 cells
Caco-2 ccl20:luc reporter system
Immune response
Anticoagulant
title_short The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
title_full The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
title_fullStr The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
title_full_unstemmed The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
title_sort The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
dc.creator.none.fl_str_mv Assad, Sabrina
Fragomeno, Melisa
Rumbo, Martín
Minnaard, Jessica
Pérez, Pablo Fernando
author Assad, Sabrina
author_facet Assad, Sabrina
Fragomeno, Melisa
Rumbo, Martín
Minnaard, Jessica
Pérez, Pablo Fernando
author_role author
author2 Fragomeno, Melisa
Rumbo, Martín
Minnaard, Jessica
Pérez, Pablo Fernando
author2_role author
author
author
author
dc.subject.none.fl_str_mv Química
Bifidobacterium
Acenocoumarol
THP-1 cells
Caco-2 ccl20:luc reporter system
Immune response
Anticoagulant
topic Química
Bifidobacterium
Acenocoumarol
THP-1 cells
Caco-2 ccl20:luc reporter system
Immune response
Anticoagulant
dc.description.none.fl_txt_mv Bifidobacteria are health promoting microorganisms that can interact with drugs in the intestinal tract thus modifying the activation of immune cells and host homeostasis. To study the effect of acenocoumarol on the interaction between bifidobacteria and THP-1 cells or intestinal epithelial cells. Bifidobacterium bifidum CIDCA 5310 and B. adolescentis CIDCA 5317 were grown in MRS broth in anaerobic conditions. The experiments were done in the presence of acenocoumarol. FITC-labeled bacteria were incubated with THP1 cells differentiated with PMA (THP1-PMA), and phagocytosis and intracellular localization of bacteria were assessed by flow cytometry and confocal laser microscopy, respectively. Also, expression of macrophage surface molecules in THP1 and THP1-PMA cells was assessed by flow cytometry. By using the Caco-2 ccl20:luc reporter system innate immune response was analysed by determining luciferase expression induced by flagellin. Acenocoumarol decreased phagocytosis of bifidobacteria but intracellular routing was not modified. Also, anticoagulant reduced the expression of HLA-DR and CD64 induced by bifidobacteria in both THP1-PMA and THP1 cells. Instead, the decrease in the expression of CD206 and CD23 was dependent on differentiation status of THP1 cells. Acenocoumarol increased CCL20 expression and it was partially abrogated by strain CIDCA 5310. Presence of acenocoumarol modifies the interaction between bifidobacteria and eukaryotic cells thus suggesting that the expected modulation of immune response by probiotic foods can be different in persons under anticoagulant treatment.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Instituto de Estudios Inmunológicos y Fisiopatológicos
description Bifidobacteria are health promoting microorganisms that can interact with drugs in the intestinal tract thus modifying the activation of immune cells and host homeostasis. To study the effect of acenocoumarol on the interaction between bifidobacteria and THP-1 cells or intestinal epithelial cells. Bifidobacterium bifidum CIDCA 5310 and B. adolescentis CIDCA 5317 were grown in MRS broth in anaerobic conditions. The experiments were done in the presence of acenocoumarol. FITC-labeled bacteria were incubated with THP1 cells differentiated with PMA (THP1-PMA), and phagocytosis and intracellular localization of bacteria were assessed by flow cytometry and confocal laser microscopy, respectively. Also, expression of macrophage surface molecules in THP1 and THP1-PMA cells was assessed by flow cytometry. By using the Caco-2 ccl20:luc reporter system innate immune response was analysed by determining luciferase expression induced by flagellin. Acenocoumarol decreased phagocytosis of bifidobacteria but intracellular routing was not modified. Also, anticoagulant reduced the expression of HLA-DR and CD64 induced by bifidobacteria in both THP1-PMA and THP1 cells. Instead, the decrease in the expression of CD206 and CD23 was dependent on differentiation status of THP1 cells. Acenocoumarol increased CCL20 expression and it was partially abrogated by strain CIDCA 5310. Presence of acenocoumarol modifies the interaction between bifidobacteria and eukaryotic cells thus suggesting that the expected modulation of immune response by probiotic foods can be different in persons under anticoagulant treatment.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/166688
url http://sedici.unlp.edu.ar/handle/10915/166688
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2212-4292
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fbio.2023.102473
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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