Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response

Autores
Crispo, Martina; Van Maele, Laurye; Tabareau, Julien; Cayet, Delphine; Errea, Agustina Juliana; Ferreira, Ana María; Rumbo, Martin; Sirard, Jean Claude
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The chemokine CCL20, the unique ligand of CCR6 functions as an attractant of immune cells. Expression of CCL20 is induced by Toll-like Receptor (TLR) signaling or proinflammatory cytokine stimulation. However CCL20 is also constitutively produced at specific epithelial sites of mucosa. This expression profile is achieved by transcriptional regulation. In the present work we characterized regulatory features of mouse Ccl20 gene. Transcriptional fusions between the mouse Ccl20 promoter and the firefly luciferase (luc) encoding gene were constructed and assessed in in vitro and in vivo assays. We found that liver CCL20 expression and luciferase activity were upregulated by systemic administration of the TLR5 agonist flagellin. Using shRNA and dominant negative form specific for mouse TLR5, we showed that this expression was controlled by TLR5. To address in situ the regulation of gene activity, a transgenic mouse line harboring a functional Ccl20-luc fusion was generated. The luciferase expression was highly concordant with Ccl20 expression in different tissues. Our data indicate that the transgenic mouse model can be used to monitor activation of innate response in vivo.
Fil: Crispo, Martina. Unidad de Animales Transgénicos y de Experimentación – Institut Pasteur de Montevideo; Uruguay
Fil: Van Maele, Laurye. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; Francia
Fil: Tabareau, Julien. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; Francia
Fil: Cayet, Delphine. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; Francia
Fil: Errea, Agustina Juliana. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Laboratorio de Invest.del Sistema Inmune; Argentina
Fil: Ferreira, Ana María. Cátedra de Inmunologíaa. Facultad de Ciencias/Facultad de Química. Universidad de la República; Uruguay
Fil: Rumbo, Martin. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Laboratorio de Invest.del Sistema Inmune; Argentina
Fil: Sirard, Jean Claude. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; Francia
Materia
CCL20
luciferase reporter
transgenic mice
liver
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19660
Acceder
id CONICETDig_0be961e352250cd49846bd1d8244d222
oai_identifier_str oai:ri.conicet.gov.ar:11336/19660
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory responseCrispo, MartinaVan Maele, LauryeTabareau, JulienCayet, DelphineErrea, Agustina JulianaFerreira, Ana MaríaRumbo, MartinSirard, Jean ClaudeCCL20luciferase reportertransgenic miceliverhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The chemokine CCL20, the unique ligand of CCR6 functions as an attractant of immune cells. Expression of CCL20 is induced by Toll-like Receptor (TLR) signaling or proinflammatory cytokine stimulation. However CCL20 is also constitutively produced at specific epithelial sites of mucosa. This expression profile is achieved by transcriptional regulation. In the present work we characterized regulatory features of mouse Ccl20 gene. Transcriptional fusions between the mouse Ccl20 promoter and the firefly luciferase (luc) encoding gene were constructed and assessed in in vitro and in vivo assays. We found that liver CCL20 expression and luciferase activity were upregulated by systemic administration of the TLR5 agonist flagellin. Using shRNA and dominant negative form specific for mouse TLR5, we showed that this expression was controlled by TLR5. To address in situ the regulation of gene activity, a transgenic mouse line harboring a functional Ccl20-luc fusion was generated. The luciferase expression was highly concordant with Ccl20 expression in different tissues. Our data indicate that the transgenic mouse model can be used to monitor activation of innate response in vivo.Fil: Crispo, Martina. Unidad de Animales Transgénicos y de Experimentación – Institut Pasteur de Montevideo; UruguayFil: Van Maele, Laurye. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; FranciaFil: Tabareau, Julien. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; FranciaFil: Cayet, Delphine. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; FranciaFil: Errea, Agustina Juliana. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Laboratorio de Invest.del Sistema Inmune; ArgentinaFil: Ferreira, Ana María. Cátedra de Inmunologíaa. Facultad de Ciencias/Facultad de Química. Universidad de la República; UruguayFil: Rumbo, Martin. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Laboratorio de Invest.del Sistema Inmune; ArgentinaFil: Sirard, Jean Claude. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; FranciaPublic Library Science2013-11-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19660Crispo, Martina; Van Maele, Laurye; Tabareau, Julien; Cayet, Delphine; Errea, Agustina Juliana; et al.; Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response; Public Library Science; Plos One; 8; 11; 12-11-2013; 13-161932-6203enginfo:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0078447info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0078447info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:29Zoai:ri.conicet.gov.ar:11336/19660instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:29.628CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response
title Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response
spellingShingle Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response
Crispo, Martina
CCL20
luciferase reporter
transgenic mice
liver
title_short Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response
title_full Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response
title_fullStr Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response
title_full_unstemmed Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response
title_sort Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response
dc.creator.none.fl_str_mv Crispo, Martina
Van Maele, Laurye
Tabareau, Julien
Cayet, Delphine
Errea, Agustina Juliana
Ferreira, Ana María
Rumbo, Martin
Sirard, Jean Claude
author Crispo, Martina
author_facet Crispo, Martina
Van Maele, Laurye
Tabareau, Julien
Cayet, Delphine
Errea, Agustina Juliana
Ferreira, Ana María
Rumbo, Martin
Sirard, Jean Claude
author_role author
author2 Van Maele, Laurye
Tabareau, Julien
Cayet, Delphine
Errea, Agustina Juliana
Ferreira, Ana María
Rumbo, Martin
Sirard, Jean Claude
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CCL20
luciferase reporter
transgenic mice
liver
topic CCL20
luciferase reporter
transgenic mice
liver
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The chemokine CCL20, the unique ligand of CCR6 functions as an attractant of immune cells. Expression of CCL20 is induced by Toll-like Receptor (TLR) signaling or proinflammatory cytokine stimulation. However CCL20 is also constitutively produced at specific epithelial sites of mucosa. This expression profile is achieved by transcriptional regulation. In the present work we characterized regulatory features of mouse Ccl20 gene. Transcriptional fusions between the mouse Ccl20 promoter and the firefly luciferase (luc) encoding gene were constructed and assessed in in vitro and in vivo assays. We found that liver CCL20 expression and luciferase activity were upregulated by systemic administration of the TLR5 agonist flagellin. Using shRNA and dominant negative form specific for mouse TLR5, we showed that this expression was controlled by TLR5. To address in situ the regulation of gene activity, a transgenic mouse line harboring a functional Ccl20-luc fusion was generated. The luciferase expression was highly concordant with Ccl20 expression in different tissues. Our data indicate that the transgenic mouse model can be used to monitor activation of innate response in vivo.
Fil: Crispo, Martina. Unidad de Animales Transgénicos y de Experimentación – Institut Pasteur de Montevideo; Uruguay
Fil: Van Maele, Laurye. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; Francia
Fil: Tabareau, Julien. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; Francia
Fil: Cayet, Delphine. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; Francia
Fil: Errea, Agustina Juliana. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Laboratorio de Invest.del Sistema Inmune; Argentina
Fil: Ferreira, Ana María. Cátedra de Inmunologíaa. Facultad de Ciencias/Facultad de Química. Universidad de la República; Uruguay
Fil: Rumbo, Martin. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Laboratorio de Invest.del Sistema Inmune; Argentina
Fil: Sirard, Jean Claude. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; Francia
description The chemokine CCL20, the unique ligand of CCR6 functions as an attractant of immune cells. Expression of CCL20 is induced by Toll-like Receptor (TLR) signaling or proinflammatory cytokine stimulation. However CCL20 is also constitutively produced at specific epithelial sites of mucosa. This expression profile is achieved by transcriptional regulation. In the present work we characterized regulatory features of mouse Ccl20 gene. Transcriptional fusions between the mouse Ccl20 promoter and the firefly luciferase (luc) encoding gene were constructed and assessed in in vitro and in vivo assays. We found that liver CCL20 expression and luciferase activity were upregulated by systemic administration of the TLR5 agonist flagellin. Using shRNA and dominant negative form specific for mouse TLR5, we showed that this expression was controlled by TLR5. To address in situ the regulation of gene activity, a transgenic mouse line harboring a functional Ccl20-luc fusion was generated. The luciferase expression was highly concordant with Ccl20 expression in different tissues. Our data indicate that the transgenic mouse model can be used to monitor activation of innate response in vivo.
publishDate 2013
dc.date.none.fl_str_mv 2013-11-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19660
Crispo, Martina; Van Maele, Laurye; Tabareau, Julien; Cayet, Delphine; Errea, Agustina Juliana; et al.; Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response; Public Library Science; Plos One; 8; 11; 12-11-2013; 13-16
1932-6203
url http://hdl.handle.net/11336/19660
identifier_str_mv Crispo, Martina; Van Maele, Laurye; Tabareau, Julien; Cayet, Delphine; Errea, Agustina Juliana; et al.; Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response; Public Library Science; Plos One; 8; 11; 12-11-2013; 13-16
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0078447
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0078447
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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