Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars
- Autores
- Brelles Mariño, Graciela; Boiardi, José Luis
- Año de publicación
- 1992
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Lectins from two Phaseolus vulgaris L. cultivars were isolated and purified by salt fractionation, affinity chromatography and gel permeation chromatography. The cultivars used were: ‘alubia’, with a low-nodulating ability, and Bat 76 with a good symbiotic aptitude. Differences in properties of the two lectins were noted: ‘alubia’ lectin gave only one peak with haemagglutinating activity following gel permeation chromatography while Bat 76 yielded two active peaks, although both lectins had several bands of about 30 kDa following gel electrophoresis, Bat 76 lecting had three bands of about 50 kDa which were not present in ‘alubia’ and red kidney bean lectins. Peptide-mapping, by limited proteolysis and two dimensional gel electrophoresis, also showed differences between the lectins which are therefore judged to be different.
Centro de Investigación y Desarrollo en Fermentaciones Industriales - Materia
-
Biología
Electrophoretic pattern
Haemagglutinating activity
Lectins
Phaseolus vulgaris - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/143261
Ver los metadatos del registro completo
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Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivarsBrelles Mariño, GracielaBoiardi, José LuisBiologíaElectrophoretic patternHaemagglutinating activityLectinsPhaseolus vulgarisLectins from two Phaseolus vulgaris L. cultivars were isolated and purified by salt fractionation, affinity chromatography and gel permeation chromatography. The cultivars used were: ‘alubia’, with a low-nodulating ability, and Bat 76 with a good symbiotic aptitude. Differences in properties of the two lectins were noted: ‘alubia’ lectin gave only one peak with haemagglutinating activity following gel permeation chromatography while Bat 76 yielded two active peaks, although both lectins had several bands of about 30 kDa following gel electrophoresis, Bat 76 lecting had three bands of about 50 kDa which were not present in ‘alubia’ and red kidney bean lectins. Peptide-mapping, by limited proteolysis and two dimensional gel electrophoresis, also showed differences between the lectins which are therefore judged to be different.Centro de Investigación y Desarrollo en Fermentaciones Industriales1992info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf573-578http://sedici.unlp.edu.ar/handle/10915/143261enginfo:eu-repo/semantics/altIdentifier/issn/0959-3993info:eu-repo/semantics/altIdentifier/issn/1573-0972info:eu-repo/semantics/altIdentifier/doi/10.1007/bf01238791info:eu-repo/semantics/altIdentifier/pmid/24425602info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:04:18Zoai:sedici.unlp.edu.ar:10915/143261Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:04:18.485SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars |
| title |
Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars |
| spellingShingle |
Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars Brelles Mariño, Graciela Biología Electrophoretic pattern Haemagglutinating activity Lectins Phaseolus vulgaris |
| title_short |
Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars |
| title_full |
Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars |
| title_fullStr |
Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars |
| title_full_unstemmed |
Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars |
| title_sort |
Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars |
| dc.creator.none.fl_str_mv |
Brelles Mariño, Graciela Boiardi, José Luis |
| author |
Brelles Mariño, Graciela |
| author_facet |
Brelles Mariño, Graciela Boiardi, José Luis |
| author_role |
author |
| author2 |
Boiardi, José Luis |
| author2_role |
author |
| dc.subject.none.fl_str_mv |
Biología Electrophoretic pattern Haemagglutinating activity Lectins Phaseolus vulgaris |
| topic |
Biología Electrophoretic pattern Haemagglutinating activity Lectins Phaseolus vulgaris |
| dc.description.none.fl_txt_mv |
Lectins from two Phaseolus vulgaris L. cultivars were isolated and purified by salt fractionation, affinity chromatography and gel permeation chromatography. The cultivars used were: ‘alubia’, with a low-nodulating ability, and Bat 76 with a good symbiotic aptitude. Differences in properties of the two lectins were noted: ‘alubia’ lectin gave only one peak with haemagglutinating activity following gel permeation chromatography while Bat 76 yielded two active peaks, although both lectins had several bands of about 30 kDa following gel electrophoresis, Bat 76 lecting had three bands of about 50 kDa which were not present in ‘alubia’ and red kidney bean lectins. Peptide-mapping, by limited proteolysis and two dimensional gel electrophoresis, also showed differences between the lectins which are therefore judged to be different. Centro de Investigación y Desarrollo en Fermentaciones Industriales |
| description |
Lectins from two Phaseolus vulgaris L. cultivars were isolated and purified by salt fractionation, affinity chromatography and gel permeation chromatography. The cultivars used were: ‘alubia’, with a low-nodulating ability, and Bat 76 with a good symbiotic aptitude. Differences in properties of the two lectins were noted: ‘alubia’ lectin gave only one peak with haemagglutinating activity following gel permeation chromatography while Bat 76 yielded two active peaks, although both lectins had several bands of about 30 kDa following gel electrophoresis, Bat 76 lecting had three bands of about 50 kDa which were not present in ‘alubia’ and red kidney bean lectins. Peptide-mapping, by limited proteolysis and two dimensional gel electrophoresis, also showed differences between the lectins which are therefore judged to be different. |
| publishDate |
1992 |
| dc.date.none.fl_str_mv |
1992 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/143261 |
| url |
http://sedici.unlp.edu.ar/handle/10915/143261 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/issn/0959-3993 info:eu-repo/semantics/altIdentifier/issn/1573-0972 info:eu-repo/semantics/altIdentifier/doi/10.1007/bf01238791 info:eu-repo/semantics/altIdentifier/pmid/24425602 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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application/pdf 573-578 |
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