Extraction of protein concentrate from red bean (Phaseolus vulgaris L.): Antioxidant activity and inhibition of lipid peroxidation

Autores
Piñuel, Maria Lucrecia; Vilcacundo, Edgar; Boeri, Patricia; Barrio, Daniel Alejandro; Morales, Dayana; Pinto, Adelita; Moran, Roberto; Samaniego, Ivan; Carrillo, Wilman
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Red Bean Protein Concentrate (RBPC) and their hydrolysates were used to evaluate the antioxidant capacity. The RBPC protein content was in the range of 57.38%-72.68% of the total sample content. RBPC protein profile showed a range of 15-100 kDa. Phaseolin protein was identified with bands of 45 and 50 kDa. Phaseolin protein was found in all the RBPC samples at the different pHs assayed. In the gastric digestion phase, bands from 60 to 100 kDa were totally hydrolyzed with pepsin. Phaseolin protein (45 and 50 kDa) presented resistance to gastric hydrolysis. All the RBPCs and gastrointestinal digest presented antioxidant activity using ferric-reducing antioxidant power (FRAP), 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), oxygen radical absorbance capacity (ORAC), and thiobarbituric acid reactive substances using the in vitro and in vivo methods. RBPC at pH 7.0 presented a value of 95.80 μmoL TE/g of RBPC (FRAP); 257.12 μmoL TE/g of RBPC (ABTS), and 1960 μmoL TE/g of RBPC (ORAC). Duodenal digest of RBPC presented high antioxidant activity with 225.77 μmoL TE/g of digest (FRAP); 345.21 μmoL TE/g of digest (ABTS); and 3256 μmoL TE/g of digest (ORAC). Gastric and duodenal digest of RBPC were used to inhibit lipid peroxidation using the in vitro method presenting a value of 87.95% and 93.0%, respectively. When the in vivo method in zebrafish larvae was used, values were 79.03% and 86.76%, respectively. RBPCs showed no reactive oxygen species (ROS) inhibition. However, RBPCs with gastric and gastrointestinal digests, presented ROS inhibition, 75.30% for gastric digests and 66.40% for gastrointestinal digests.
Fil: Piñuel, Maria Lucrecia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Negro; Argentina
Fil: Vilcacundo, Edgar. Universidad Estatal de Bolivar; Ecuador
Fil: Boeri, Patricia. Universidad Nacional de Río Negro; Argentina
Fil: Barrio, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Negro; Argentina
Fil: Morales, Dayana. Universidad Técnica de Ambato; Ecuador
Fil: Pinto, Adelita. Universidad Técnica de Babahoyo; Ecuador
Fil: Moran, Roberto. Universidad Nacional de Río Negro; Argentina
Fil: Samaniego, Ivan. Instituto Nacional de Investigaciones Agropecuarias; Ecuador
Fil: Carrillo, Wilman. Universidad Técnica de Babahoyo; Ecuador
Materia
ANTIOXIDANT ACTIVITY
COMMON BEAN
PHASEOLUS VULGARIS
PROTEIN CONCENTRATE
ZEBRAFISH LARVAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/175069
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/175069
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Extraction of protein concentrate from red bean (Phaseolus vulgaris L.): Antioxidant activity and inhibition of lipid peroxidationPiñuel, Maria LucreciaVilcacundo, EdgarBoeri, PatriciaBarrio, Daniel AlejandroMorales, DayanaPinto, AdelitaMoran, RobertoSamaniego, IvanCarrillo, WilmanANTIOXIDANT ACTIVITYCOMMON BEANPHASEOLUS VULGARISPROTEIN CONCENTRATEZEBRAFISH LARVAEhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Red Bean Protein Concentrate (RBPC) and their hydrolysates were used to evaluate the antioxidant capacity. The RBPC protein content was in the range of 57.38%-72.68% of the total sample content. RBPC protein profile showed a range of 15-100 kDa. Phaseolin protein was identified with bands of 45 and 50 kDa. Phaseolin protein was found in all the RBPC samples at the different pHs assayed. In the gastric digestion phase, bands from 60 to 100 kDa were totally hydrolyzed with pepsin. Phaseolin protein (45 and 50 kDa) presented resistance to gastric hydrolysis. All the RBPCs and gastrointestinal digest presented antioxidant activity using ferric-reducing antioxidant power (FRAP), 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), oxygen radical absorbance capacity (ORAC), and thiobarbituric acid reactive substances using the in vitro and in vivo methods. RBPC at pH 7.0 presented a value of 95.80 μmoL TE/g of RBPC (FRAP); 257.12 μmoL TE/g of RBPC (ABTS), and 1960 μmoL TE/g of RBPC (ORAC). Duodenal digest of RBPC presented high antioxidant activity with 225.77 μmoL TE/g of digest (FRAP); 345.21 μmoL TE/g of digest (ABTS); and 3256 μmoL TE/g of digest (ORAC). Gastric and duodenal digest of RBPC were used to inhibit lipid peroxidation using the in vitro method presenting a value of 87.95% and 93.0%, respectively. When the in vivo method in zebrafish larvae was used, values were 79.03% and 86.76%, respectively. RBPCs showed no reactive oxygen species (ROS) inhibition. However, RBPCs with gastric and gastrointestinal digests, presented ROS inhibition, 75.30% for gastric digests and 66.40% for gastrointestinal digests.Fil: Piñuel, Maria Lucrecia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Negro; ArgentinaFil: Vilcacundo, Edgar. Universidad Estatal de Bolivar; EcuadorFil: Boeri, Patricia. Universidad Nacional de Río Negro; ArgentinaFil: Barrio, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Negro; ArgentinaFil: Morales, Dayana. Universidad Técnica de Ambato; EcuadorFil: Pinto, Adelita. Universidad Técnica de Babahoyo; EcuadorFil: Moran, Roberto. Universidad Nacional de Río Negro; ArgentinaFil: Samaniego, Ivan. Instituto Nacional de Investigaciones Agropecuarias; EcuadorFil: Carrillo, Wilman. Universidad Técnica de Babahoyo; EcuadorOpen Science Publishers LLP Inc.2019-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/175069Piñuel, Maria Lucrecia; Vilcacundo, Edgar; Boeri, Patricia; Barrio, Daniel Alejandro; Morales, Dayana; et al.; Extraction of protein concentrate from red bean (Phaseolus vulgaris L.): Antioxidant activity and inhibition of lipid peroxidation; Open Science Publishers LLP Inc.; Journal of Applied Pharmaceutical Science; 9; 9; 9-2019; 45-582231-3354CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://japsonline.com/abstract.php?article_id=2978&sts=2info:eu-repo/semantics/altIdentifier/doi/10.7324/JAPS.2019.90804info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:27Zoai:ri.conicet.gov.ar:11336/175069instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:27.975CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Extraction of protein concentrate from red bean (Phaseolus vulgaris L.): Antioxidant activity and inhibition of lipid peroxidation
title Extraction of protein concentrate from red bean (Phaseolus vulgaris L.): Antioxidant activity and inhibition of lipid peroxidation
spellingShingle Extraction of protein concentrate from red bean (Phaseolus vulgaris L.): Antioxidant activity and inhibition of lipid peroxidation
Piñuel, Maria Lucrecia
ANTIOXIDANT ACTIVITY
COMMON BEAN
PHASEOLUS VULGARIS
PROTEIN CONCENTRATE
ZEBRAFISH LARVAE
title_short Extraction of protein concentrate from red bean (Phaseolus vulgaris L.): Antioxidant activity and inhibition of lipid peroxidation
title_full Extraction of protein concentrate from red bean (Phaseolus vulgaris L.): Antioxidant activity and inhibition of lipid peroxidation
title_fullStr Extraction of protein concentrate from red bean (Phaseolus vulgaris L.): Antioxidant activity and inhibition of lipid peroxidation
title_full_unstemmed Extraction of protein concentrate from red bean (Phaseolus vulgaris L.): Antioxidant activity and inhibition of lipid peroxidation
title_sort Extraction of protein concentrate from red bean (Phaseolus vulgaris L.): Antioxidant activity and inhibition of lipid peroxidation
dc.creator.none.fl_str_mv Piñuel, Maria Lucrecia
Vilcacundo, Edgar
Boeri, Patricia
Barrio, Daniel Alejandro
Morales, Dayana
Pinto, Adelita
Moran, Roberto
Samaniego, Ivan
Carrillo, Wilman
author Piñuel, Maria Lucrecia
author_facet Piñuel, Maria Lucrecia
Vilcacundo, Edgar
Boeri, Patricia
Barrio, Daniel Alejandro
Morales, Dayana
Pinto, Adelita
Moran, Roberto
Samaniego, Ivan
Carrillo, Wilman
author_role author
author2 Vilcacundo, Edgar
Boeri, Patricia
Barrio, Daniel Alejandro
Morales, Dayana
Pinto, Adelita
Moran, Roberto
Samaniego, Ivan
Carrillo, Wilman
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIOXIDANT ACTIVITY
COMMON BEAN
PHASEOLUS VULGARIS
PROTEIN CONCENTRATE
ZEBRAFISH LARVAE
topic ANTIOXIDANT ACTIVITY
COMMON BEAN
PHASEOLUS VULGARIS
PROTEIN CONCENTRATE
ZEBRAFISH LARVAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Red Bean Protein Concentrate (RBPC) and their hydrolysates were used to evaluate the antioxidant capacity. The RBPC protein content was in the range of 57.38%-72.68% of the total sample content. RBPC protein profile showed a range of 15-100 kDa. Phaseolin protein was identified with bands of 45 and 50 kDa. Phaseolin protein was found in all the RBPC samples at the different pHs assayed. In the gastric digestion phase, bands from 60 to 100 kDa were totally hydrolyzed with pepsin. Phaseolin protein (45 and 50 kDa) presented resistance to gastric hydrolysis. All the RBPCs and gastrointestinal digest presented antioxidant activity using ferric-reducing antioxidant power (FRAP), 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), oxygen radical absorbance capacity (ORAC), and thiobarbituric acid reactive substances using the in vitro and in vivo methods. RBPC at pH 7.0 presented a value of 95.80 μmoL TE/g of RBPC (FRAP); 257.12 μmoL TE/g of RBPC (ABTS), and 1960 μmoL TE/g of RBPC (ORAC). Duodenal digest of RBPC presented high antioxidant activity with 225.77 μmoL TE/g of digest (FRAP); 345.21 μmoL TE/g of digest (ABTS); and 3256 μmoL TE/g of digest (ORAC). Gastric and duodenal digest of RBPC were used to inhibit lipid peroxidation using the in vitro method presenting a value of 87.95% and 93.0%, respectively. When the in vivo method in zebrafish larvae was used, values were 79.03% and 86.76%, respectively. RBPCs showed no reactive oxygen species (ROS) inhibition. However, RBPCs with gastric and gastrointestinal digests, presented ROS inhibition, 75.30% for gastric digests and 66.40% for gastrointestinal digests.
Fil: Piñuel, Maria Lucrecia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Negro; Argentina
Fil: Vilcacundo, Edgar. Universidad Estatal de Bolivar; Ecuador
Fil: Boeri, Patricia. Universidad Nacional de Río Negro; Argentina
Fil: Barrio, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Negro; Argentina
Fil: Morales, Dayana. Universidad Técnica de Ambato; Ecuador
Fil: Pinto, Adelita. Universidad Técnica de Babahoyo; Ecuador
Fil: Moran, Roberto. Universidad Nacional de Río Negro; Argentina
Fil: Samaniego, Ivan. Instituto Nacional de Investigaciones Agropecuarias; Ecuador
Fil: Carrillo, Wilman. Universidad Técnica de Babahoyo; Ecuador
description Red Bean Protein Concentrate (RBPC) and their hydrolysates were used to evaluate the antioxidant capacity. The RBPC protein content was in the range of 57.38%-72.68% of the total sample content. RBPC protein profile showed a range of 15-100 kDa. Phaseolin protein was identified with bands of 45 and 50 kDa. Phaseolin protein was found in all the RBPC samples at the different pHs assayed. In the gastric digestion phase, bands from 60 to 100 kDa were totally hydrolyzed with pepsin. Phaseolin protein (45 and 50 kDa) presented resistance to gastric hydrolysis. All the RBPCs and gastrointestinal digest presented antioxidant activity using ferric-reducing antioxidant power (FRAP), 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), oxygen radical absorbance capacity (ORAC), and thiobarbituric acid reactive substances using the in vitro and in vivo methods. RBPC at pH 7.0 presented a value of 95.80 μmoL TE/g of RBPC (FRAP); 257.12 μmoL TE/g of RBPC (ABTS), and 1960 μmoL TE/g of RBPC (ORAC). Duodenal digest of RBPC presented high antioxidant activity with 225.77 μmoL TE/g of digest (FRAP); 345.21 μmoL TE/g of digest (ABTS); and 3256 μmoL TE/g of digest (ORAC). Gastric and duodenal digest of RBPC were used to inhibit lipid peroxidation using the in vitro method presenting a value of 87.95% and 93.0%, respectively. When the in vivo method in zebrafish larvae was used, values were 79.03% and 86.76%, respectively. RBPCs showed no reactive oxygen species (ROS) inhibition. However, RBPCs with gastric and gastrointestinal digests, presented ROS inhibition, 75.30% for gastric digests and 66.40% for gastrointestinal digests.
publishDate 2019
dc.date.none.fl_str_mv 2019-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/175069
Piñuel, Maria Lucrecia; Vilcacundo, Edgar; Boeri, Patricia; Barrio, Daniel Alejandro; Morales, Dayana; et al.; Extraction of protein concentrate from red bean (Phaseolus vulgaris L.): Antioxidant activity and inhibition of lipid peroxidation; Open Science Publishers LLP Inc.; Journal of Applied Pharmaceutical Science; 9; 9; 9-2019; 45-58
2231-3354
CONICET Digital
CONICET
url http://hdl.handle.net/11336/175069
identifier_str_mv Piñuel, Maria Lucrecia; Vilcacundo, Edgar; Boeri, Patricia; Barrio, Daniel Alejandro; Morales, Dayana; et al.; Extraction of protein concentrate from red bean (Phaseolus vulgaris L.): Antioxidant activity and inhibition of lipid peroxidation; Open Science Publishers LLP Inc.; Journal of Applied Pharmaceutical Science; 9; 9; 9-2019; 45-58
2231-3354
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://japsonline.com/abstract.php?article_id=2978&sts=2
info:eu-repo/semantics/altIdentifier/doi/10.7324/JAPS.2019.90804
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Open Science Publishers LLP Inc.
publisher.none.fl_str_mv Open Science Publishers LLP Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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