Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
- Autores
- Rudzinski, Marcelo; Pardini, Lais Luján; Bernstein, Mariana; Moré, Gastón Andrés; Khoury, Marina; Duarte, Silvana Carolina; Argüelles, Carina Francisca; Reina, Silvia; Oubiña, Jose Raul
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL.
Facultad de Ciencias Veterinarias - Materia
-
Biología
Ciencias Médicas
Ocular toxoplasmosis
Cytokine release - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/145951
Ver los metadatos del registro completo
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Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosisRudzinski, MarceloPardini, Lais LujánBernstein, MarianaMoré, Gastón AndrésKhoury, MarinaDuarte, Silvana CarolinaArgüelles, Carina FranciscaReina, SilviaOubiña, Jose RaulBiologíaCiencias MédicasOcular toxoplasmosisCytokine releasePeripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL.Facultad de Ciencias Veterinarias2020-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf2239-2243http://sedici.unlp.edu.ar/handle/10915/145951enginfo:eu-repo/semantics/altIdentifier/issn/1476-1645info:eu-repo/semantics/altIdentifier/issn/0002-9637info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.20-0124info:eu-repo/semantics/altIdentifier/pmid/32901605info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:04:28Zoai:sedici.unlp.edu.ar:10915/145951Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:04:28.375SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis |
title |
Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis |
spellingShingle |
Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis Rudzinski, Marcelo Biología Ciencias Médicas Ocular toxoplasmosis Cytokine release |
title_short |
Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis |
title_full |
Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis |
title_fullStr |
Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis |
title_full_unstemmed |
Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis |
title_sort |
Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis |
dc.creator.none.fl_str_mv |
Rudzinski, Marcelo Pardini, Lais Luján Bernstein, Mariana Moré, Gastón Andrés Khoury, Marina Duarte, Silvana Carolina Argüelles, Carina Francisca Reina, Silvia Oubiña, Jose Raul |
author |
Rudzinski, Marcelo |
author_facet |
Rudzinski, Marcelo Pardini, Lais Luján Bernstein, Mariana Moré, Gastón Andrés Khoury, Marina Duarte, Silvana Carolina Argüelles, Carina Francisca Reina, Silvia Oubiña, Jose Raul |
author_role |
author |
author2 |
Pardini, Lais Luján Bernstein, Mariana Moré, Gastón Andrés Khoury, Marina Duarte, Silvana Carolina Argüelles, Carina Francisca Reina, Silvia Oubiña, Jose Raul |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Biología Ciencias Médicas Ocular toxoplasmosis Cytokine release |
topic |
Biología Ciencias Médicas Ocular toxoplasmosis Cytokine release |
dc.description.none.fl_txt_mv |
Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL. Facultad de Ciencias Veterinarias |
description |
Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/145951 |
url |
http://sedici.unlp.edu.ar/handle/10915/145951 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/1476-1645 info:eu-repo/semantics/altIdentifier/issn/0002-9637 info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.20-0124 info:eu-repo/semantics/altIdentifier/pmid/32901605 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf 2239-2243 |
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