Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis

Autores
Rudzinski, Marcelo; Pardini, Lais Luján; Bernstein, Mariana; Moré, Gastón Andrés; Khoury, Marina; Duarte, Silvana Carolina; Argüelles, Carina Francisca; Reina, Silvia; Oubiña, Jose Raul
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL.
Facultad de Ciencias Veterinarias
Materia
Biología
Ciencias Médicas
Ocular toxoplasmosis
Cytokine release
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/145951
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/145951
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosisRudzinski, MarceloPardini, Lais LujánBernstein, MarianaMoré, Gastón AndrésKhoury, MarinaDuarte, Silvana CarolinaArgüelles, Carina FranciscaReina, SilviaOubiña, Jose RaulBiologíaCiencias MédicasOcular toxoplasmosisCytokine releasePeripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL.Facultad de Ciencias Veterinarias2020-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf2239-2243http://sedici.unlp.edu.ar/handle/10915/145951enginfo:eu-repo/semantics/altIdentifier/issn/1476-1645info:eu-repo/semantics/altIdentifier/issn/0002-9637info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.20-0124info:eu-repo/semantics/altIdentifier/pmid/32901605info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:21Zoai:sedici.unlp.edu.ar:10915/145951Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:22.223SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
title Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
spellingShingle Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
Rudzinski, Marcelo
Biología
Ciencias Médicas
Ocular toxoplasmosis
Cytokine release
title_short Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
title_full Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
title_fullStr Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
title_full_unstemmed Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
title_sort Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
dc.creator.none.fl_str_mv Rudzinski, Marcelo
Pardini, Lais Luján
Bernstein, Mariana
Moré, Gastón Andrés
Khoury, Marina
Duarte, Silvana Carolina
Argüelles, Carina Francisca
Reina, Silvia
Oubiña, Jose Raul
author Rudzinski, Marcelo
author_facet Rudzinski, Marcelo
Pardini, Lais Luján
Bernstein, Mariana
Moré, Gastón Andrés
Khoury, Marina
Duarte, Silvana Carolina
Argüelles, Carina Francisca
Reina, Silvia
Oubiña, Jose Raul
author_role author
author2 Pardini, Lais Luján
Bernstein, Mariana
Moré, Gastón Andrés
Khoury, Marina
Duarte, Silvana Carolina
Argüelles, Carina Francisca
Reina, Silvia
Oubiña, Jose Raul
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Ciencias Médicas
Ocular toxoplasmosis
Cytokine release
topic Biología
Ciencias Médicas
Ocular toxoplasmosis
Cytokine release
dc.description.none.fl_txt_mv Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL.
Facultad de Ciencias Veterinarias
description Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL.
publishDate 2020
dc.date.none.fl_str_mv 2020-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/145951
url http://sedici.unlp.edu.ar/handle/10915/145951
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1476-1645
info:eu-repo/semantics/altIdentifier/issn/0002-9637
info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.20-0124
info:eu-repo/semantics/altIdentifier/pmid/32901605
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
2239-2243
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.069144

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