Apoptosis and cell proliferation in porcine placental vascularization

Autores
Sanchis, Eva G.; Cristofolini, Andrea L.; Fiorimanti, Mariana R.; Barbeito, Claudio Gustavo; Merkis, Cecilia I.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The placenta is a highly vascularized organ, indispensable tothe transfer of nutrients to the growing fetuses. During gestation, there exists an expansion of the placental vascular network through active angiogenesis. The aim of this research was to study cell proliferation and apoptosis through high resolution light microscopy (HRLM) and transmission electron microscopy (TEM) ultrastructure, immunohistochemistry for Ki67and caspase-3, determination of placental vascular area,and TUNEL assay. Crossbred sows placental tissues from approximately 30 ± 2(n =5), 40 ± 2(n =5), 60 ± 2 (n =5), 80 ± 2(n =5), 90 ± 2(n =5) and 114 ± 2(n =5) days of gestation were used. The evaluation of cell proliferation showed the highest%Ki67 values on days 30 ± 2 and 80 ± 2 of pregnancy. Caspase-3 expressed the highest value on day 30 ± 2, while the highest apoptotic indexes were found on days30 ± 2 and 90 ± 2. The placental vascular area was higher on day 80 ± 2 of pregnancy. According to our results, an active vascular cell remodeling by a caspase-3 dependent apoptosis seems to be present in early pregnancy. The increase in the vascular area on day 80 ± 2 would be the result of the intense vascular cell proliferation detected with Ki67. Further studies are needed to understand the complex processes of angiogenesis, cell proliferation and apoptosis that interact in the placenta during porcine gestation.
Facultad de Ciencias Veterinarias
Materia
Veterinaria
Apoptosis
pigs
Placenta
ultrastructure
vascularization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/105785

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network_name_str SEDICI (UNLP)
spelling Apoptosis and cell proliferation in porcine placental vascularizationSanchis, Eva G.Cristofolini, Andrea L.Fiorimanti, Mariana R.Barbeito, Claudio GustavoMerkis, Cecilia I.VeterinariaApoptosispigsPlacentaultrastructurevascularizationThe placenta is a highly vascularized organ, indispensable tothe transfer of nutrients to the growing fetuses. During gestation, there exists an expansion of the placental vascular network through active angiogenesis. The aim of this research was to study cell proliferation and apoptosis through high resolution light microscopy (HRLM) and transmission electron microscopy (TEM) ultrastructure, immunohistochemistry for Ki67and caspase-3, determination of placental vascular area,and TUNEL assay. Crossbred sows placental tissues from approximately 30 ± 2(n =5), 40 ± 2(n =5), 60 ± 2 (n =5), 80 ± 2(n =5), 90 ± 2(n =5) and 114 ± 2(n =5) days of gestation were used. The evaluation of cell proliferation showed the highest%Ki67 values on days 30 ± 2 and 80 ± 2 of pregnancy. Caspase-3 expressed the highest value on day 30 ± 2, while the highest apoptotic indexes were found on days30 ± 2 and 90 ± 2. The placental vascular area was higher on day 80 ± 2 of pregnancy. According to our results, an active vascular cell remodeling by a caspase-3 dependent apoptosis seems to be present in early pregnancy. The increase in the vascular area on day 80 ± 2 would be the result of the intense vascular cell proliferation detected with Ki67. Further studies are needed to understand the complex processes of angiogenesis, cell proliferation and apoptosis that interact in the placenta during porcine gestation.Facultad de Ciencias Veterinarias2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf20-28http://sedici.unlp.edu.ar/handle/10915/105785enginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0378432017300945info:eu-repo/semantics/altIdentifier/issn/0378-4320info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2017.06.009info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:15:32Zoai:sedici.unlp.edu.ar:10915/105785Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:15:33.201SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Apoptosis and cell proliferation in porcine placental vascularization
title Apoptosis and cell proliferation in porcine placental vascularization
spellingShingle Apoptosis and cell proliferation in porcine placental vascularization
Sanchis, Eva G.
Veterinaria
Apoptosis
pigs
Placenta
ultrastructure
vascularization
title_short Apoptosis and cell proliferation in porcine placental vascularization
title_full Apoptosis and cell proliferation in porcine placental vascularization
title_fullStr Apoptosis and cell proliferation in porcine placental vascularization
title_full_unstemmed Apoptosis and cell proliferation in porcine placental vascularization
title_sort Apoptosis and cell proliferation in porcine placental vascularization
dc.creator.none.fl_str_mv Sanchis, Eva G.
Cristofolini, Andrea L.
Fiorimanti, Mariana R.
Barbeito, Claudio Gustavo
Merkis, Cecilia I.
author Sanchis, Eva G.
author_facet Sanchis, Eva G.
Cristofolini, Andrea L.
Fiorimanti, Mariana R.
Barbeito, Claudio Gustavo
Merkis, Cecilia I.
author_role author
author2 Cristofolini, Andrea L.
Fiorimanti, Mariana R.
Barbeito, Claudio Gustavo
Merkis, Cecilia I.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Veterinaria
Apoptosis
pigs
Placenta
ultrastructure
vascularization
topic Veterinaria
Apoptosis
pigs
Placenta
ultrastructure
vascularization
dc.description.none.fl_txt_mv The placenta is a highly vascularized organ, indispensable tothe transfer of nutrients to the growing fetuses. During gestation, there exists an expansion of the placental vascular network through active angiogenesis. The aim of this research was to study cell proliferation and apoptosis through high resolution light microscopy (HRLM) and transmission electron microscopy (TEM) ultrastructure, immunohistochemistry for Ki67and caspase-3, determination of placental vascular area,and TUNEL assay. Crossbred sows placental tissues from approximately 30 ± 2(n =5), 40 ± 2(n =5), 60 ± 2 (n =5), 80 ± 2(n =5), 90 ± 2(n =5) and 114 ± 2(n =5) days of gestation were used. The evaluation of cell proliferation showed the highest%Ki67 values on days 30 ± 2 and 80 ± 2 of pregnancy. Caspase-3 expressed the highest value on day 30 ± 2, while the highest apoptotic indexes were found on days30 ± 2 and 90 ± 2. The placental vascular area was higher on day 80 ± 2 of pregnancy. According to our results, an active vascular cell remodeling by a caspase-3 dependent apoptosis seems to be present in early pregnancy. The increase in the vascular area on day 80 ± 2 would be the result of the intense vascular cell proliferation detected with Ki67. Further studies are needed to understand the complex processes of angiogenesis, cell proliferation and apoptosis that interact in the placenta during porcine gestation.
Facultad de Ciencias Veterinarias
description The placenta is a highly vascularized organ, indispensable tothe transfer of nutrients to the growing fetuses. During gestation, there exists an expansion of the placental vascular network through active angiogenesis. The aim of this research was to study cell proliferation and apoptosis through high resolution light microscopy (HRLM) and transmission electron microscopy (TEM) ultrastructure, immunohistochemistry for Ki67and caspase-3, determination of placental vascular area,and TUNEL assay. Crossbred sows placental tissues from approximately 30 ± 2(n =5), 40 ± 2(n =5), 60 ± 2 (n =5), 80 ± 2(n =5), 90 ± 2(n =5) and 114 ± 2(n =5) days of gestation were used. The evaluation of cell proliferation showed the highest%Ki67 values on days 30 ± 2 and 80 ± 2 of pregnancy. Caspase-3 expressed the highest value on day 30 ± 2, while the highest apoptotic indexes were found on days30 ± 2 and 90 ± 2. The placental vascular area was higher on day 80 ± 2 of pregnancy. According to our results, an active vascular cell remodeling by a caspase-3 dependent apoptosis seems to be present in early pregnancy. The increase in the vascular area on day 80 ± 2 would be the result of the intense vascular cell proliferation detected with Ki67. Further studies are needed to understand the complex processes of angiogenesis, cell proliferation and apoptosis that interact in the placenta during porcine gestation.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/105785
url http://sedici.unlp.edu.ar/handle/10915/105785
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0378432017300945
info:eu-repo/semantics/altIdentifier/issn/0378-4320
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2017.06.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
20-28
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
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reponame_str SEDICI (UNLP)
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institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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