Apoptosis and cell proliferation in porcine placental vascularization
- Autores
- Sanchis, Eva G.; Cristofolini, Andrea L.; Fiorimanti, Mariana R.; Barbeito, Claudio Gustavo; Merkis, Cecilia I.
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The placenta is a highly vascularized organ, indispensable tothe transfer of nutrients to the growing fetuses. During gestation, there exists an expansion of the placental vascular network through active angiogenesis. The aim of this research was to study cell proliferation and apoptosis through high resolution light microscopy (HRLM) and transmission electron microscopy (TEM) ultrastructure, immunohistochemistry for Ki67and caspase-3, determination of placental vascular area,and TUNEL assay. Crossbred sows placental tissues from approximately 30 ± 2(n =5), 40 ± 2(n =5), 60 ± 2 (n =5), 80 ± 2(n =5), 90 ± 2(n =5) and 114 ± 2(n =5) days of gestation were used. The evaluation of cell proliferation showed the highest%Ki67 values on days 30 ± 2 and 80 ± 2 of pregnancy. Caspase-3 expressed the highest value on day 30 ± 2, while the highest apoptotic indexes were found on days30 ± 2 and 90 ± 2. The placental vascular area was higher on day 80 ± 2 of pregnancy. According to our results, an active vascular cell remodeling by a caspase-3 dependent apoptosis seems to be present in early pregnancy. The increase in the vascular area on day 80 ± 2 would be the result of the intense vascular cell proliferation detected with Ki67. Further studies are needed to understand the complex processes of angiogenesis, cell proliferation and apoptosis that interact in the placenta during porcine gestation.
Facultad de Ciencias Veterinarias - Materia
-
Veterinaria
Apoptosis
pigs
Placenta
ultrastructure
vascularization - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/105785
Ver los metadatos del registro completo
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Apoptosis and cell proliferation in porcine placental vascularizationSanchis, Eva G.Cristofolini, Andrea L.Fiorimanti, Mariana R.Barbeito, Claudio GustavoMerkis, Cecilia I.VeterinariaApoptosispigsPlacentaultrastructurevascularizationThe placenta is a highly vascularized organ, indispensable tothe transfer of nutrients to the growing fetuses. During gestation, there exists an expansion of the placental vascular network through active angiogenesis. The aim of this research was to study cell proliferation and apoptosis through high resolution light microscopy (HRLM) and transmission electron microscopy (TEM) ultrastructure, immunohistochemistry for Ki67and caspase-3, determination of placental vascular area,and TUNEL assay. Crossbred sows placental tissues from approximately 30 ± 2(n =5), 40 ± 2(n =5), 60 ± 2 (n =5), 80 ± 2(n =5), 90 ± 2(n =5) and 114 ± 2(n =5) days of gestation were used. The evaluation of cell proliferation showed the highest%Ki67 values on days 30 ± 2 and 80 ± 2 of pregnancy. Caspase-3 expressed the highest value on day 30 ± 2, while the highest apoptotic indexes were found on days30 ± 2 and 90 ± 2. The placental vascular area was higher on day 80 ± 2 of pregnancy. According to our results, an active vascular cell remodeling by a caspase-3 dependent apoptosis seems to be present in early pregnancy. The increase in the vascular area on day 80 ± 2 would be the result of the intense vascular cell proliferation detected with Ki67. Further studies are needed to understand the complex processes of angiogenesis, cell proliferation and apoptosis that interact in the placenta during porcine gestation.Facultad de Ciencias Veterinarias2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf20-28http://sedici.unlp.edu.ar/handle/10915/105785enginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0378432017300945info:eu-repo/semantics/altIdentifier/issn/0378-4320info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2017.06.009info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:15:32Zoai:sedici.unlp.edu.ar:10915/105785Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:15:33.201SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Apoptosis and cell proliferation in porcine placental vascularization |
title |
Apoptosis and cell proliferation in porcine placental vascularization |
spellingShingle |
Apoptosis and cell proliferation in porcine placental vascularization Sanchis, Eva G. Veterinaria Apoptosis pigs Placenta ultrastructure vascularization |
title_short |
Apoptosis and cell proliferation in porcine placental vascularization |
title_full |
Apoptosis and cell proliferation in porcine placental vascularization |
title_fullStr |
Apoptosis and cell proliferation in porcine placental vascularization |
title_full_unstemmed |
Apoptosis and cell proliferation in porcine placental vascularization |
title_sort |
Apoptosis and cell proliferation in porcine placental vascularization |
dc.creator.none.fl_str_mv |
Sanchis, Eva G. Cristofolini, Andrea L. Fiorimanti, Mariana R. Barbeito, Claudio Gustavo Merkis, Cecilia I. |
author |
Sanchis, Eva G. |
author_facet |
Sanchis, Eva G. Cristofolini, Andrea L. Fiorimanti, Mariana R. Barbeito, Claudio Gustavo Merkis, Cecilia I. |
author_role |
author |
author2 |
Cristofolini, Andrea L. Fiorimanti, Mariana R. Barbeito, Claudio Gustavo Merkis, Cecilia I. |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Veterinaria Apoptosis pigs Placenta ultrastructure vascularization |
topic |
Veterinaria Apoptosis pigs Placenta ultrastructure vascularization |
dc.description.none.fl_txt_mv |
The placenta is a highly vascularized organ, indispensable tothe transfer of nutrients to the growing fetuses. During gestation, there exists an expansion of the placental vascular network through active angiogenesis. The aim of this research was to study cell proliferation and apoptosis through high resolution light microscopy (HRLM) and transmission electron microscopy (TEM) ultrastructure, immunohistochemistry for Ki67and caspase-3, determination of placental vascular area,and TUNEL assay. Crossbred sows placental tissues from approximately 30 ± 2(n =5), 40 ± 2(n =5), 60 ± 2 (n =5), 80 ± 2(n =5), 90 ± 2(n =5) and 114 ± 2(n =5) days of gestation were used. The evaluation of cell proliferation showed the highest%Ki67 values on days 30 ± 2 and 80 ± 2 of pregnancy. Caspase-3 expressed the highest value on day 30 ± 2, while the highest apoptotic indexes were found on days30 ± 2 and 90 ± 2. The placental vascular area was higher on day 80 ± 2 of pregnancy. According to our results, an active vascular cell remodeling by a caspase-3 dependent apoptosis seems to be present in early pregnancy. The increase in the vascular area on day 80 ± 2 would be the result of the intense vascular cell proliferation detected with Ki67. Further studies are needed to understand the complex processes of angiogenesis, cell proliferation and apoptosis that interact in the placenta during porcine gestation. Facultad de Ciencias Veterinarias |
description |
The placenta is a highly vascularized organ, indispensable tothe transfer of nutrients to the growing fetuses. During gestation, there exists an expansion of the placental vascular network through active angiogenesis. The aim of this research was to study cell proliferation and apoptosis through high resolution light microscopy (HRLM) and transmission electron microscopy (TEM) ultrastructure, immunohistochemistry for Ki67and caspase-3, determination of placental vascular area,and TUNEL assay. Crossbred sows placental tissues from approximately 30 ± 2(n =5), 40 ± 2(n =5), 60 ± 2 (n =5), 80 ± 2(n =5), 90 ± 2(n =5) and 114 ± 2(n =5) days of gestation were used. The evaluation of cell proliferation showed the highest%Ki67 values on days 30 ± 2 and 80 ± 2 of pregnancy. Caspase-3 expressed the highest value on day 30 ± 2, while the highest apoptotic indexes were found on days30 ± 2 and 90 ± 2. The placental vascular area was higher on day 80 ± 2 of pregnancy. According to our results, an active vascular cell remodeling by a caspase-3 dependent apoptosis seems to be present in early pregnancy. The increase in the vascular area on day 80 ± 2 would be the result of the intense vascular cell proliferation detected with Ki67. Further studies are needed to understand the complex processes of angiogenesis, cell proliferation and apoptosis that interact in the placenta during porcine gestation. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/105785 |
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http://sedici.unlp.edu.ar/handle/10915/105785 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
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