Production of Pectinolytic Enzymes by the Yeast <i>Wickerhanomyces anomalus</i> Isolated from Citrus Fruits Peels

Autores
Martos, María Alicia; Zubreski, Emilce; Garro, Oscar Alfredo; Hours, Roque Alberto
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Wickerhamomyces anomalus is pectinolytic yeast isolated from citrus fruits peels in the province of Misiones, Argentine. In the present work, enzymes produced by this yeast strain were characterized, and polygalacturonase physicochemical properties were determined in order to evaluate the application of the supernatant in the maceration of potato tissues. W. anomalus was able to produce PG in liquid medium containing glucose and citrus pectin, whose mode of action was mainly of endo type. The supernatant did not exhibit esterase or lyase activity. No others enzymes, capable of hydrolyzing cell wall polymers, such as cellulases and xylanases, were detected. PG showed maximal activity at pH 4.5 and at temperature range between 40°C and 50°C. It was stable in the pH range from 3.0 to 6.0 and up to 50°C at optimum pH. The enzymatic extract macerated potato tissues efficiently. Volume of single cells increased with the agitation speed. The results observed make the enzymatic extract produced by W. anomalus appropriate for future application in food industry, mainly for the production of fruit nectars or mashed of vegetables such as potato or cassava, of regional interest in the province of Misiones, Argentine.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
Wickerhamomyces anomalus
Pectinolytic enzymes
Fermentación
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/3.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/104647

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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Production of Pectinolytic Enzymes by the Yeast <i>Wickerhanomyces anomalus</i> Isolated from Citrus Fruits PeelsMartos, María AliciaZubreski, EmilceGarro, Oscar AlfredoHours, Roque AlbertoCiencias ExactasWickerhamomyces anomalusPectinolytic enzymesFermentación<i>Wickerhamomyces anomalus</i> is pectinolytic yeast isolated from citrus fruits peels in the province of Misiones, Argentine. In the present work, enzymes produced by this yeast strain were characterized, and polygalacturonase physicochemical properties were determined in order to evaluate the application of the supernatant in the maceration of potato tissues. <i>W. anomalus</i> was able to produce PG in liquid medium containing glucose and citrus pectin, whose mode of action was mainly of endo type. The supernatant did not exhibit esterase or lyase activity. No others enzymes, capable of hydrolyzing cell wall polymers, such as cellulases and xylanases, were detected. PG showed maximal activity at pH 4.5 and at temperature range between 40°C and 50°C. It was stable in the pH range from 3.0 to 6.0 and up to 50°C at optimum pH. The enzymatic extract macerated potato tissues efficiently. Volume of single cells increased with the agitation speed. The results observed make the enzymatic extract produced by <i>W. anomalus</i> appropriate for future application in food industry, mainly for the production of fruit nectars or mashed of vegetables such as potato or cassava, of regional interest in the province of Misiones, Argentine.Centro de Investigación y Desarrollo en Fermentaciones Industriales2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/104647enginfo:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/4311info:eu-repo/semantics/altIdentifier/issn/2090-3138info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/435154info:eu-repo/semantics/altIdentifier/hdl/11336/4311info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/Creative Commons Attribution 3.0 Unported (CC BY 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:54:55Zoai:sedici.unlp.edu.ar:10915/104647Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:54:56.202SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Production of Pectinolytic Enzymes by the Yeast <i>Wickerhanomyces anomalus</i> Isolated from Citrus Fruits Peels
title Production of Pectinolytic Enzymes by the Yeast <i>Wickerhanomyces anomalus</i> Isolated from Citrus Fruits Peels
spellingShingle Production of Pectinolytic Enzymes by the Yeast <i>Wickerhanomyces anomalus</i> Isolated from Citrus Fruits Peels
Martos, María Alicia
Ciencias Exactas
Wickerhamomyces anomalus
Pectinolytic enzymes
Fermentación
title_short Production of Pectinolytic Enzymes by the Yeast <i>Wickerhanomyces anomalus</i> Isolated from Citrus Fruits Peels
title_full Production of Pectinolytic Enzymes by the Yeast <i>Wickerhanomyces anomalus</i> Isolated from Citrus Fruits Peels
title_fullStr Production of Pectinolytic Enzymes by the Yeast <i>Wickerhanomyces anomalus</i> Isolated from Citrus Fruits Peels
title_full_unstemmed Production of Pectinolytic Enzymes by the Yeast <i>Wickerhanomyces anomalus</i> Isolated from Citrus Fruits Peels
title_sort Production of Pectinolytic Enzymes by the Yeast <i>Wickerhanomyces anomalus</i> Isolated from Citrus Fruits Peels
dc.creator.none.fl_str_mv Martos, María Alicia
Zubreski, Emilce
Garro, Oscar Alfredo
Hours, Roque Alberto
author Martos, María Alicia
author_facet Martos, María Alicia
Zubreski, Emilce
Garro, Oscar Alfredo
Hours, Roque Alberto
author_role author
author2 Zubreski, Emilce
Garro, Oscar Alfredo
Hours, Roque Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Wickerhamomyces anomalus
Pectinolytic enzymes
Fermentación
topic Ciencias Exactas
Wickerhamomyces anomalus
Pectinolytic enzymes
Fermentación
dc.description.none.fl_txt_mv <i>Wickerhamomyces anomalus</i> is pectinolytic yeast isolated from citrus fruits peels in the province of Misiones, Argentine. In the present work, enzymes produced by this yeast strain were characterized, and polygalacturonase physicochemical properties were determined in order to evaluate the application of the supernatant in the maceration of potato tissues. <i>W. anomalus</i> was able to produce PG in liquid medium containing glucose and citrus pectin, whose mode of action was mainly of endo type. The supernatant did not exhibit esterase or lyase activity. No others enzymes, capable of hydrolyzing cell wall polymers, such as cellulases and xylanases, were detected. PG showed maximal activity at pH 4.5 and at temperature range between 40°C and 50°C. It was stable in the pH range from 3.0 to 6.0 and up to 50°C at optimum pH. The enzymatic extract macerated potato tissues efficiently. Volume of single cells increased with the agitation speed. The results observed make the enzymatic extract produced by <i>W. anomalus</i> appropriate for future application in food industry, mainly for the production of fruit nectars or mashed of vegetables such as potato or cassava, of regional interest in the province of Misiones, Argentine.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description <i>Wickerhamomyces anomalus</i> is pectinolytic yeast isolated from citrus fruits peels in the province of Misiones, Argentine. In the present work, enzymes produced by this yeast strain were characterized, and polygalacturonase physicochemical properties were determined in order to evaluate the application of the supernatant in the maceration of potato tissues. <i>W. anomalus</i> was able to produce PG in liquid medium containing glucose and citrus pectin, whose mode of action was mainly of endo type. The supernatant did not exhibit esterase or lyase activity. No others enzymes, capable of hydrolyzing cell wall polymers, such as cellulases and xylanases, were detected. PG showed maximal activity at pH 4.5 and at temperature range between 40°C and 50°C. It was stable in the pH range from 3.0 to 6.0 and up to 50°C at optimum pH. The enzymatic extract macerated potato tissues efficiently. Volume of single cells increased with the agitation speed. The results observed make the enzymatic extract produced by <i>W. anomalus</i> appropriate for future application in food industry, mainly for the production of fruit nectars or mashed of vegetables such as potato or cassava, of regional interest in the province of Misiones, Argentine.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/104647
url http://sedici.unlp.edu.ar/handle/10915/104647
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/4311
info:eu-repo/semantics/altIdentifier/issn/2090-3138
info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/435154
info:eu-repo/semantics/altIdentifier/hdl/11336/4311
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/
Creative Commons Attribution 3.0 Unported (CC BY 3.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/
Creative Commons Attribution 3.0 Unported (CC BY 3.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
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instname_str Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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