Exploration of heavy metal resistance in the yeast Wickerhamomyces anomalus: Implications for bioremediation

Autores
Garolera, Betsabé; Pajot, Hipolito Fernando; Fernandez, Pablo Marcelo
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The inadequate disposal of wastewater containing toxic heavy metals and industrial contaminants has become a critical issue today, posing serious risks to the health of humans, animals, and the environment. Numerous studies have investigated how heavy metals are absorbed, accumulated, and transformed using microorganisms. The yeast strain Wickerhamomyces anomalus M10 has demonstrated notable resistance to several heavy metals, showing promising potential for bioremediation. Therefore, through computational methods (In Silico studies) to investigate metal resistance mechanisms, relevant genes related to this resistance were identified. Specialized databases like BacMet and InterPro were used, compiling genes from bacteria and fungi that enhance survival under environmental stress. These databases categorize resistance genes based on their function and induced phenotypes. Using BacMet, we identified 59 genes, including 11 ABC-type transporters, 10 related to resistance and the efflux of multiple drugs, as well as proteins for copper (5), nickel (10), arsenic (2), mercury (5), and silver (1). These proteins perform functions in binding, reduction, and transport, along with superoxide dismutase proteins, DNA regulation, and repair genes. Additionally, through InterPro, we found 394 genes, including 257 transporters, 25 copper-related proteins, and 23 zinc-related proteins. Fifteen multidrug resistance proteins and other DNA regulation and repair genes (10) were detected. In summary, our bioinformatics analysis of Wickerhamomyces anomalus highlights its potential for bioremediation in metal-contaminated environments. It excels particularly in mitigating nickel and arsenic pollution due to resistance-related genes. However, comprehensive in vivo studies are required to confirm its survival and transformation capabilities against these metals. These findings underline the significant bioremediation potential of yeast strains. The study not only reveals genes that expand the scope of microbial bioremediation but also advances the concept of the substantial role of yeast strains in mitigating environmental pollution challenges.
Fil: Garolera, Betsabé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData
Rosario
Argentina
Argentine Association of Bioinformatics and Computational Biology
Materia
Bioinformatic
Wickerhamomyces anomalus
Heavy metals
Bioremediation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237241
Acceder
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spelling Exploration of heavy metal resistance in the yeast Wickerhamomyces anomalus: Implications for bioremediationGarolera, BetsabéPajot, Hipolito FernandoFernandez, Pablo MarceloBioinformaticWickerhamomyces anomalusHeavy metalsBioremediationhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The inadequate disposal of wastewater containing toxic heavy metals and industrial contaminants has become a critical issue today, posing serious risks to the health of humans, animals, and the environment. Numerous studies have investigated how heavy metals are absorbed, accumulated, and transformed using microorganisms. The yeast strain Wickerhamomyces anomalus M10 has demonstrated notable resistance to several heavy metals, showing promising potential for bioremediation. Therefore, through computational methods (In Silico studies) to investigate metal resistance mechanisms, relevant genes related to this resistance were identified. Specialized databases like BacMet and InterPro were used, compiling genes from bacteria and fungi that enhance survival under environmental stress. These databases categorize resistance genes based on their function and induced phenotypes. Using BacMet, we identified 59 genes, including 11 ABC-type transporters, 10 related to resistance and the efflux of multiple drugs, as well as proteins for copper (5), nickel (10), arsenic (2), mercury (5), and silver (1). These proteins perform functions in binding, reduction, and transport, along with superoxide dismutase proteins, DNA regulation, and repair genes. Additionally, through InterPro, we found 394 genes, including 257 transporters, 25 copper-related proteins, and 23 zinc-related proteins. Fifteen multidrug resistance proteins and other DNA regulation and repair genes (10) were detected. In summary, our bioinformatics analysis of Wickerhamomyces anomalus highlights its potential for bioremediation in metal-contaminated environments. It excels particularly in mitigating nickel and arsenic pollution due to resistance-related genes. However, comprehensive in vivo studies are required to confirm its survival and transformation capabilities against these metals. These findings underline the significant bioremediation potential of yeast strains. The study not only reveals genes that expand the scope of microbial bioremediation but also advances the concept of the substantial role of yeast strains in mitigating environmental pollution challenges.Fil: Garolera, Betsabé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; ArgentinaXIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioDataRosarioArgentinaArgentine Association of Bioinformatics and Computational BiologyArgentine Association of Bioinformatics and Computational Biology2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/237241Exploration of heavy metal resistance in the yeast Wickerhamomyces anomalus: Implications for bioremediation; XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData; Rosario; Argentina; 2023; 67-67978-987-48989-7-5CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://2023.a2b2c.org.ar/BookOfAbstracts2023.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:47Zoai:ri.conicet.gov.ar:11336/237241instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:47.743CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Exploration of heavy metal resistance in the yeast Wickerhamomyces anomalus: Implications for bioremediation
title Exploration of heavy metal resistance in the yeast Wickerhamomyces anomalus: Implications for bioremediation
spellingShingle Exploration of heavy metal resistance in the yeast Wickerhamomyces anomalus: Implications for bioremediation
Garolera, Betsabé
Bioinformatic
Wickerhamomyces anomalus
Heavy metals
Bioremediation
title_short Exploration of heavy metal resistance in the yeast Wickerhamomyces anomalus: Implications for bioremediation
title_full Exploration of heavy metal resistance in the yeast Wickerhamomyces anomalus: Implications for bioremediation
title_fullStr Exploration of heavy metal resistance in the yeast Wickerhamomyces anomalus: Implications for bioremediation
title_full_unstemmed Exploration of heavy metal resistance in the yeast Wickerhamomyces anomalus: Implications for bioremediation
title_sort Exploration of heavy metal resistance in the yeast Wickerhamomyces anomalus: Implications for bioremediation
dc.creator.none.fl_str_mv Garolera, Betsabé
Pajot, Hipolito Fernando
Fernandez, Pablo Marcelo
author Garolera, Betsabé
author_facet Garolera, Betsabé
Pajot, Hipolito Fernando
Fernandez, Pablo Marcelo
author_role author
author2 Pajot, Hipolito Fernando
Fernandez, Pablo Marcelo
author2_role author
author
dc.subject.none.fl_str_mv Bioinformatic
Wickerhamomyces anomalus
Heavy metals
Bioremediation
topic Bioinformatic
Wickerhamomyces anomalus
Heavy metals
Bioremediation
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The inadequate disposal of wastewater containing toxic heavy metals and industrial contaminants has become a critical issue today, posing serious risks to the health of humans, animals, and the environment. Numerous studies have investigated how heavy metals are absorbed, accumulated, and transformed using microorganisms. The yeast strain Wickerhamomyces anomalus M10 has demonstrated notable resistance to several heavy metals, showing promising potential for bioremediation. Therefore, through computational methods (In Silico studies) to investigate metal resistance mechanisms, relevant genes related to this resistance were identified. Specialized databases like BacMet and InterPro were used, compiling genes from bacteria and fungi that enhance survival under environmental stress. These databases categorize resistance genes based on their function and induced phenotypes. Using BacMet, we identified 59 genes, including 11 ABC-type transporters, 10 related to resistance and the efflux of multiple drugs, as well as proteins for copper (5), nickel (10), arsenic (2), mercury (5), and silver (1). These proteins perform functions in binding, reduction, and transport, along with superoxide dismutase proteins, DNA regulation, and repair genes. Additionally, through InterPro, we found 394 genes, including 257 transporters, 25 copper-related proteins, and 23 zinc-related proteins. Fifteen multidrug resistance proteins and other DNA regulation and repair genes (10) were detected. In summary, our bioinformatics analysis of Wickerhamomyces anomalus highlights its potential for bioremediation in metal-contaminated environments. It excels particularly in mitigating nickel and arsenic pollution due to resistance-related genes. However, comprehensive in vivo studies are required to confirm its survival and transformation capabilities against these metals. These findings underline the significant bioremediation potential of yeast strains. The study not only reveals genes that expand the scope of microbial bioremediation but also advances the concept of the substantial role of yeast strains in mitigating environmental pollution challenges.
Fil: Garolera, Betsabé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData
Rosario
Argentina
Argentine Association of Bioinformatics and Computational Biology
description The inadequate disposal of wastewater containing toxic heavy metals and industrial contaminants has become a critical issue today, posing serious risks to the health of humans, animals, and the environment. Numerous studies have investigated how heavy metals are absorbed, accumulated, and transformed using microorganisms. The yeast strain Wickerhamomyces anomalus M10 has demonstrated notable resistance to several heavy metals, showing promising potential for bioremediation. Therefore, through computational methods (In Silico studies) to investigate metal resistance mechanisms, relevant genes related to this resistance were identified. Specialized databases like BacMet and InterPro were used, compiling genes from bacteria and fungi that enhance survival under environmental stress. These databases categorize resistance genes based on their function and induced phenotypes. Using BacMet, we identified 59 genes, including 11 ABC-type transporters, 10 related to resistance and the efflux of multiple drugs, as well as proteins for copper (5), nickel (10), arsenic (2), mercury (5), and silver (1). These proteins perform functions in binding, reduction, and transport, along with superoxide dismutase proteins, DNA regulation, and repair genes. Additionally, through InterPro, we found 394 genes, including 257 transporters, 25 copper-related proteins, and 23 zinc-related proteins. Fifteen multidrug resistance proteins and other DNA regulation and repair genes (10) were detected. In summary, our bioinformatics analysis of Wickerhamomyces anomalus highlights its potential for bioremediation in metal-contaminated environments. It excels particularly in mitigating nickel and arsenic pollution due to resistance-related genes. However, comprehensive in vivo studies are required to confirm its survival and transformation capabilities against these metals. These findings underline the significant bioremediation potential of yeast strains. The study not only reveals genes that expand the scope of microbial bioremediation but also advances the concept of the substantial role of yeast strains in mitigating environmental pollution challenges.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237241
Exploration of heavy metal resistance in the yeast Wickerhamomyces anomalus: Implications for bioremediation; XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData; Rosario; Argentina; 2023; 67-67
978-987-48989-7-5
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237241
identifier_str_mv Exploration of heavy metal resistance in the yeast Wickerhamomyces anomalus: Implications for bioremediation; XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData; Rosario; Argentina; 2023; 67-67
978-987-48989-7-5
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://2023.a2b2c.org.ar/BookOfAbstracts2023.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Argentine Association of Bioinformatics and Computational Biology
publisher.none.fl_str_mv Argentine Association of Bioinformatics and Computational Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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