Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?
- Autores
- Garolera, Betsabé; Pajot, Hipolito Fernando; Fernandez, Pablo Marcelo
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Wickerhamomyces anomalus is a yeast with significant biotechnological potential, utilized in the production of fermented beverages and ethanol, as well as in the biocontrol of postharvest diseases, bioremediation of metals and organic contaminants, and surfactant production, among other applications. Its utility arises from its capacity to thrive on various carbon sources, tolerate a wide range of pH values, and survive temperatures ranging from 20 to 40°C. Nevertheless, documented cases of fungal infections in immunocompromised patients, including infants and adults, suggest its potential as an opportunistic pathogen. In our laboratory, we isolated a strain of Wickerhamomyces anomalus with potential for hexavalent chromium bioremediation. The aim of this study was to in silico assess the presence of virulence factor genes in the Wickerhamomyces anomalus M10 strain, which could confer opportunistic pathogenicity in humans. Using PHI-base, we identified a total of 65 virulence factors, categorized based on their phenotypic impact in mutations. Loss-of-pathogenicity factors (13) are primarily associated with genes involved in hyphae or pseudohyphae formation, such as Cas5, CDC42, and VMA4, whereas virulence reduction factors (37) are linked to genes related to biofilm formation, like BMH1 and RPS41. Furthermore, we identified genes that may enable the yeast to evade host defenses, such as an alpha-mannosyltransferase (MNN10), all belonging to proteins involved in signaling pathways, transport, and transcription regulation. In summary, based on this initial bioinformatic analysis of the Wickerhamomyces anomalus genome, we suggest that the yeast possesses genes that could facilitate its invasion and dissemination within the host, along with the ability to evade host defenses, potentially conferring opportunistic pathogenicity. It is crucial to conduct in vivo assays to validate these findings, given the potential risk to human health when used in biotechnological processes.
Fil: Garolera, Betsabé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData
Rosario
Argentina
Argentine Association of Bioinformatics and Computational Biology - Materia
-
Bioinformatic
Wickerhamomyces anomalus
Opportunistic Pathogen
Heavy metals - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/237238
Ver los metadatos del registro completo
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Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?Garolera, BetsabéPajot, Hipolito FernandoFernandez, Pablo MarceloBioinformaticWickerhamomyces anomalusOpportunistic PathogenHeavy metalshttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Wickerhamomyces anomalus is a yeast with significant biotechnological potential, utilized in the production of fermented beverages and ethanol, as well as in the biocontrol of postharvest diseases, bioremediation of metals and organic contaminants, and surfactant production, among other applications. Its utility arises from its capacity to thrive on various carbon sources, tolerate a wide range of pH values, and survive temperatures ranging from 20 to 40°C. Nevertheless, documented cases of fungal infections in immunocompromised patients, including infants and adults, suggest its potential as an opportunistic pathogen. In our laboratory, we isolated a strain of Wickerhamomyces anomalus with potential for hexavalent chromium bioremediation. The aim of this study was to in silico assess the presence of virulence factor genes in the Wickerhamomyces anomalus M10 strain, which could confer opportunistic pathogenicity in humans. Using PHI-base, we identified a total of 65 virulence factors, categorized based on their phenotypic impact in mutations. Loss-of-pathogenicity factors (13) are primarily associated with genes involved in hyphae or pseudohyphae formation, such as Cas5, CDC42, and VMA4, whereas virulence reduction factors (37) are linked to genes related to biofilm formation, like BMH1 and RPS41. Furthermore, we identified genes that may enable the yeast to evade host defenses, such as an alpha-mannosyltransferase (MNN10), all belonging to proteins involved in signaling pathways, transport, and transcription regulation. In summary, based on this initial bioinformatic analysis of the Wickerhamomyces anomalus genome, we suggest that the yeast possesses genes that could facilitate its invasion and dissemination within the host, along with the ability to evade host defenses, potentially conferring opportunistic pathogenicity. It is crucial to conduct in vivo assays to validate these findings, given the potential risk to human health when used in biotechnological processes.Fil: Garolera, Betsabé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; ArgentinaXIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioDataRosarioArgentinaArgentine Association of Bioinformatics and Computational BiologyArgentine Association of Bioinformatics and Computational Biology2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/237238Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?; XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData; Rosario; Argentina; 2023; 78-78CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://2023.a2b2c.org.ar/BookOfAbstracts2023.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:03Zoai:ri.conicet.gov.ar:11336/237238instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:04.006CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen? |
title |
Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen? |
spellingShingle |
Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen? Garolera, Betsabé Bioinformatic Wickerhamomyces anomalus Opportunistic Pathogen Heavy metals |
title_short |
Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen? |
title_full |
Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen? |
title_fullStr |
Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen? |
title_full_unstemmed |
Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen? |
title_sort |
Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen? |
dc.creator.none.fl_str_mv |
Garolera, Betsabé Pajot, Hipolito Fernando Fernandez, Pablo Marcelo |
author |
Garolera, Betsabé |
author_facet |
Garolera, Betsabé Pajot, Hipolito Fernando Fernandez, Pablo Marcelo |
author_role |
author |
author2 |
Pajot, Hipolito Fernando Fernandez, Pablo Marcelo |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Bioinformatic Wickerhamomyces anomalus Opportunistic Pathogen Heavy metals |
topic |
Bioinformatic Wickerhamomyces anomalus Opportunistic Pathogen Heavy metals |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.8 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Wickerhamomyces anomalus is a yeast with significant biotechnological potential, utilized in the production of fermented beverages and ethanol, as well as in the biocontrol of postharvest diseases, bioremediation of metals and organic contaminants, and surfactant production, among other applications. Its utility arises from its capacity to thrive on various carbon sources, tolerate a wide range of pH values, and survive temperatures ranging from 20 to 40°C. Nevertheless, documented cases of fungal infections in immunocompromised patients, including infants and adults, suggest its potential as an opportunistic pathogen. In our laboratory, we isolated a strain of Wickerhamomyces anomalus with potential for hexavalent chromium bioremediation. The aim of this study was to in silico assess the presence of virulence factor genes in the Wickerhamomyces anomalus M10 strain, which could confer opportunistic pathogenicity in humans. Using PHI-base, we identified a total of 65 virulence factors, categorized based on their phenotypic impact in mutations. Loss-of-pathogenicity factors (13) are primarily associated with genes involved in hyphae or pseudohyphae formation, such as Cas5, CDC42, and VMA4, whereas virulence reduction factors (37) are linked to genes related to biofilm formation, like BMH1 and RPS41. Furthermore, we identified genes that may enable the yeast to evade host defenses, such as an alpha-mannosyltransferase (MNN10), all belonging to proteins involved in signaling pathways, transport, and transcription regulation. In summary, based on this initial bioinformatic analysis of the Wickerhamomyces anomalus genome, we suggest that the yeast possesses genes that could facilitate its invasion and dissemination within the host, along with the ability to evade host defenses, potentially conferring opportunistic pathogenicity. It is crucial to conduct in vivo assays to validate these findings, given the potential risk to human health when used in biotechnological processes. Fil: Garolera, Betsabé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData Rosario Argentina Argentine Association of Bioinformatics and Computational Biology |
description |
Wickerhamomyces anomalus is a yeast with significant biotechnological potential, utilized in the production of fermented beverages and ethanol, as well as in the biocontrol of postharvest diseases, bioremediation of metals and organic contaminants, and surfactant production, among other applications. Its utility arises from its capacity to thrive on various carbon sources, tolerate a wide range of pH values, and survive temperatures ranging from 20 to 40°C. Nevertheless, documented cases of fungal infections in immunocompromised patients, including infants and adults, suggest its potential as an opportunistic pathogen. In our laboratory, we isolated a strain of Wickerhamomyces anomalus with potential for hexavalent chromium bioremediation. The aim of this study was to in silico assess the presence of virulence factor genes in the Wickerhamomyces anomalus M10 strain, which could confer opportunistic pathogenicity in humans. Using PHI-base, we identified a total of 65 virulence factors, categorized based on their phenotypic impact in mutations. Loss-of-pathogenicity factors (13) are primarily associated with genes involved in hyphae or pseudohyphae formation, such as Cas5, CDC42, and VMA4, whereas virulence reduction factors (37) are linked to genes related to biofilm formation, like BMH1 and RPS41. Furthermore, we identified genes that may enable the yeast to evade host defenses, such as an alpha-mannosyltransferase (MNN10), all belonging to proteins involved in signaling pathways, transport, and transcription regulation. In summary, based on this initial bioinformatic analysis of the Wickerhamomyces anomalus genome, we suggest that the yeast possesses genes that could facilitate its invasion and dissemination within the host, along with the ability to evade host defenses, potentially conferring opportunistic pathogenicity. It is crucial to conduct in vivo assays to validate these findings, given the potential risk to human health when used in biotechnological processes. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/237238 Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?; XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData; Rosario; Argentina; 2023; 78-78 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/237238 |
identifier_str_mv |
Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?; XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData; Rosario; Argentina; 2023; 78-78 CONICET Digital CONICET |
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eng |
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eng |
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info:eu-repo/semantics/altIdentifier/url/http://2023.a2b2c.org.ar/BookOfAbstracts2023.pdf |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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Internacional |
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Argentine Association of Bioinformatics and Computational Biology |
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Argentine Association of Bioinformatics and Computational Biology |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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