Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?

Autores
Garolera, Betsabé; Pajot, Hipolito Fernando; Fernandez, Pablo Marcelo
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Wickerhamomyces anomalus is a yeast with significant biotechnological potential, utilized in the production of fermented beverages and ethanol, as well as in the biocontrol of postharvest diseases, bioremediation of metals and organic contaminants, and surfactant production, among other applications. Its utility arises from its capacity to thrive on various carbon sources, tolerate a wide range of pH values, and survive temperatures ranging from 20 to 40°C. Nevertheless, documented cases of fungal infections in immunocompromised patients, including infants and adults, suggest its potential as an opportunistic pathogen. In our laboratory, we isolated a strain of Wickerhamomyces anomalus with potential for hexavalent chromium bioremediation. The aim of this study was to in silico assess the presence of virulence factor genes in the Wickerhamomyces anomalus M10 strain, which could confer opportunistic pathogenicity in humans. Using PHI-base, we identified a total of 65 virulence factors, categorized based on their phenotypic impact in mutations. Loss-of-pathogenicity factors (13) are primarily associated with genes involved in hyphae or pseudohyphae formation, such as Cas5, CDC42, and VMA4, whereas virulence reduction factors (37) are linked to genes related to biofilm formation, like BMH1 and RPS41. Furthermore, we identified genes that may enable the yeast to evade host defenses, such as an alpha-mannosyltransferase (MNN10), all belonging to proteins involved in signaling pathways, transport, and transcription regulation. In summary, based on this initial bioinformatic analysis of the Wickerhamomyces anomalus genome, we suggest that the yeast possesses genes that could facilitate its invasion and dissemination within the host, along with the ability to evade host defenses, potentially conferring opportunistic pathogenicity. It is crucial to conduct in vivo assays to validate these findings, given the potential risk to human health when used in biotechnological processes.
Fil: Garolera, Betsabé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData
Rosario
Argentina
Argentine Association of Bioinformatics and Computational Biology
Materia
Bioinformatic
Wickerhamomyces anomalus
Opportunistic Pathogen
Heavy metals
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237238
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?Garolera, BetsabéPajot, Hipolito FernandoFernandez, Pablo MarceloBioinformaticWickerhamomyces anomalusOpportunistic PathogenHeavy metalshttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Wickerhamomyces anomalus is a yeast with significant biotechnological potential, utilized in the production of fermented beverages and ethanol, as well as in the biocontrol of postharvest diseases, bioremediation of metals and organic contaminants, and surfactant production, among other applications. Its utility arises from its capacity to thrive on various carbon sources, tolerate a wide range of pH values, and survive temperatures ranging from 20 to 40°C. Nevertheless, documented cases of fungal infections in immunocompromised patients, including infants and adults, suggest its potential as an opportunistic pathogen. In our laboratory, we isolated a strain of Wickerhamomyces anomalus with potential for hexavalent chromium bioremediation. The aim of this study was to in silico assess the presence of virulence factor genes in the Wickerhamomyces anomalus M10 strain, which could confer opportunistic pathogenicity in humans. Using PHI-base, we identified a total of 65 virulence factors, categorized based on their phenotypic impact in mutations. Loss-of-pathogenicity factors (13) are primarily associated with genes involved in hyphae or pseudohyphae formation, such as Cas5, CDC42, and VMA4, whereas virulence reduction factors (37) are linked to genes related to biofilm formation, like BMH1 and RPS41. Furthermore, we identified genes that may enable the yeast to evade host defenses, such as an alpha-mannosyltransferase (MNN10), all belonging to proteins involved in signaling pathways, transport, and transcription regulation. In summary, based on this initial bioinformatic analysis of the Wickerhamomyces anomalus genome, we suggest that the yeast possesses genes that could facilitate its invasion and dissemination within the host, along with the ability to evade host defenses, potentially conferring opportunistic pathogenicity. It is crucial to conduct in vivo assays to validate these findings, given the potential risk to human health when used in biotechnological processes.Fil: Garolera, Betsabé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; ArgentinaXIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioDataRosarioArgentinaArgentine Association of Bioinformatics and Computational BiologyArgentine Association of Bioinformatics and Computational Biology2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/237238Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?; XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData; Rosario; Argentina; 2023; 78-78CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://2023.a2b2c.org.ar/BookOfAbstracts2023.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:57Zoai:ri.conicet.gov.ar:11336/237238instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:58.12CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?
title Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?
spellingShingle Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?
Garolera, Betsabé
Bioinformatic
Wickerhamomyces anomalus
Opportunistic Pathogen
Heavy metals
title_short Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?
title_full Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?
title_fullStr Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?
title_full_unstemmed Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?
title_sort Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?
dc.creator.none.fl_str_mv Garolera, Betsabé
Pajot, Hipolito Fernando
Fernandez, Pablo Marcelo
author Garolera, Betsabé
author_facet Garolera, Betsabé
Pajot, Hipolito Fernando
Fernandez, Pablo Marcelo
author_role author
author2 Pajot, Hipolito Fernando
Fernandez, Pablo Marcelo
author2_role author
author
dc.subject.none.fl_str_mv Bioinformatic
Wickerhamomyces anomalus
Opportunistic Pathogen
Heavy metals
topic Bioinformatic
Wickerhamomyces anomalus
Opportunistic Pathogen
Heavy metals
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Wickerhamomyces anomalus is a yeast with significant biotechnological potential, utilized in the production of fermented beverages and ethanol, as well as in the biocontrol of postharvest diseases, bioremediation of metals and organic contaminants, and surfactant production, among other applications. Its utility arises from its capacity to thrive on various carbon sources, tolerate a wide range of pH values, and survive temperatures ranging from 20 to 40°C. Nevertheless, documented cases of fungal infections in immunocompromised patients, including infants and adults, suggest its potential as an opportunistic pathogen. In our laboratory, we isolated a strain of Wickerhamomyces anomalus with potential for hexavalent chromium bioremediation. The aim of this study was to in silico assess the presence of virulence factor genes in the Wickerhamomyces anomalus M10 strain, which could confer opportunistic pathogenicity in humans. Using PHI-base, we identified a total of 65 virulence factors, categorized based on their phenotypic impact in mutations. Loss-of-pathogenicity factors (13) are primarily associated with genes involved in hyphae or pseudohyphae formation, such as Cas5, CDC42, and VMA4, whereas virulence reduction factors (37) are linked to genes related to biofilm formation, like BMH1 and RPS41. Furthermore, we identified genes that may enable the yeast to evade host defenses, such as an alpha-mannosyltransferase (MNN10), all belonging to proteins involved in signaling pathways, transport, and transcription regulation. In summary, based on this initial bioinformatic analysis of the Wickerhamomyces anomalus genome, we suggest that the yeast possesses genes that could facilitate its invasion and dissemination within the host, along with the ability to evade host defenses, potentially conferring opportunistic pathogenicity. It is crucial to conduct in vivo assays to validate these findings, given the potential risk to human health when used in biotechnological processes.
Fil: Garolera, Betsabé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData
Rosario
Argentina
Argentine Association of Bioinformatics and Computational Biology
description Wickerhamomyces anomalus is a yeast with significant biotechnological potential, utilized in the production of fermented beverages and ethanol, as well as in the biocontrol of postharvest diseases, bioremediation of metals and organic contaminants, and surfactant production, among other applications. Its utility arises from its capacity to thrive on various carbon sources, tolerate a wide range of pH values, and survive temperatures ranging from 20 to 40°C. Nevertheless, documented cases of fungal infections in immunocompromised patients, including infants and adults, suggest its potential as an opportunistic pathogen. In our laboratory, we isolated a strain of Wickerhamomyces anomalus with potential for hexavalent chromium bioremediation. The aim of this study was to in silico assess the presence of virulence factor genes in the Wickerhamomyces anomalus M10 strain, which could confer opportunistic pathogenicity in humans. Using PHI-base, we identified a total of 65 virulence factors, categorized based on their phenotypic impact in mutations. Loss-of-pathogenicity factors (13) are primarily associated with genes involved in hyphae or pseudohyphae formation, such as Cas5, CDC42, and VMA4, whereas virulence reduction factors (37) are linked to genes related to biofilm formation, like BMH1 and RPS41. Furthermore, we identified genes that may enable the yeast to evade host defenses, such as an alpha-mannosyltransferase (MNN10), all belonging to proteins involved in signaling pathways, transport, and transcription regulation. In summary, based on this initial bioinformatic analysis of the Wickerhamomyces anomalus genome, we suggest that the yeast possesses genes that could facilitate its invasion and dissemination within the host, along with the ability to evade host defenses, potentially conferring opportunistic pathogenicity. It is crucial to conduct in vivo assays to validate these findings, given the potential risk to human health when used in biotechnological processes.
publishDate 2023
dc.date.none.fl_str_mv 2023
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info:eu-repo/semantics/conferenceObject
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Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237238
Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?; XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData; Rosario; Argentina; 2023; 78-78
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237238
identifier_str_mv Wickerhamomyces anomalus, a Biotechnological Yeast... an Opportunistic Pathogen?; XIII Argentine Congress of Bioinformatics and Computational Biology; XIII International Conference of the Iberoamerican Society of Bioinformatics; III Annual Meeting of the Ibero-American Artificial Intelligence Network for Big BioData; Rosario; Argentina; 2023; 78-78
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://2023.a2b2c.org.ar/BookOfAbstracts2023.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Argentine Association of Bioinformatics and Computational Biology
publisher.none.fl_str_mv Argentine Association of Bioinformatics and Computational Biology
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