Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set

Autores
Rogberg Muñoz, Andrés; Texeira, L. Viana; Villegas Castagnasso, Egle Etel; Teixeira, C. Salviano; Peral García, Pilar; Oliveira, D. A. Andrade De; Giovambattista, Guillermo
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Buffaloes (Bubalus bubalis) are widely distributed and were introduced to Brazil in 1895. Most of the molecular genetic characterization of buffaloes has been done with cross-specific (cattle) markers, but few of them include Brazilian populations. Nineteen commonly used cattle microsatellites were tested to develop a multiplexed set of microsatellites and characterize Brazilian buffalo. Three PCR mixes were finally developed with the 11 markers that succeed in amplify and were polymorphic (58%). The average number of alleles was 5.42, with an average observed and expected heterocigozity of 0.441 and 0.695, respectively. As it was expected, Brazilian buffalo variability was lower than the previously reported from the domestication centres (China and India), but higher than the seriously selected European populations. The exclusion power calculated for the eleven markers in Brazilian buffalo was 0.9999999996, this allows its use in DNA based traceability.
Instituto de Genética Veterinaria
Materia
Ciencias Veterinarias
Ciencias Agrarias
Buffalo
Microsatellite
Variability
Dna markers
Traceability
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/96644

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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite SetRogberg Muñoz, AndrésTexeira, L. VianaVillegas Castagnasso, Egle EtelTeixeira, C. SalvianoPeral García, PilarOliveira, D. A. Andrade DeGiovambattista, GuillermoCiencias VeterinariasCiencias AgrariasBuffaloMicrosatelliteVariabilityDna markersTraceabilityBuffaloes (Bubalus bubalis) are widely distributed and were introduced to Brazil in 1895. Most of the molecular genetic characterization of buffaloes has been done with cross-specific (cattle) markers, but few of them include Brazilian populations. Nineteen commonly used cattle microsatellites were tested to develop a multiplexed set of microsatellites and characterize Brazilian buffalo. Three PCR mixes were finally developed with the 11 markers that succeed in amplify and were polymorphic (58%). The average number of alleles was 5.42, with an average observed and expected heterocigozity of 0.441 and 0.695, respectively. As it was expected, Brazilian buffalo variability was lower than the previously reported from the domestication centres (China and India), but higher than the seriously selected European populations. The exclusion power calculated for the eleven markers in Brazilian buffalo was 0.9999999996, this allows its use in DNA based traceability.Instituto de Genética Veterinaria2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1008-1012http://sedici.unlp.edu.ar/handle/10915/96644enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/79628info:eu-repo/semantics/altIdentifier/url/http://www.davidpublisher.org/index.php/Home/Article/index?id=15917.htmlinfo:eu-repo/semantics/altIdentifier/issn/2161-6264info:eu-repo/semantics/altIdentifier/hdl/11336/79628info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:01:16Zoai:sedici.unlp.edu.ar:10915/96644Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:01:16.508SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
title Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
spellingShingle Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
Rogberg Muñoz, Andrés
Ciencias Veterinarias
Ciencias Agrarias
Buffalo
Microsatellite
Variability
Dna markers
Traceability
title_short Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
title_full Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
title_fullStr Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
title_full_unstemmed Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
title_sort Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
dc.creator.none.fl_str_mv Rogberg Muñoz, Andrés
Texeira, L. Viana
Villegas Castagnasso, Egle Etel
Teixeira, C. Salviano
Peral García, Pilar
Oliveira, D. A. Andrade De
Giovambattista, Guillermo
author Rogberg Muñoz, Andrés
author_facet Rogberg Muñoz, Andrés
Texeira, L. Viana
Villegas Castagnasso, Egle Etel
Teixeira, C. Salviano
Peral García, Pilar
Oliveira, D. A. Andrade De
Giovambattista, Guillermo
author_role author
author2 Texeira, L. Viana
Villegas Castagnasso, Egle Etel
Teixeira, C. Salviano
Peral García, Pilar
Oliveira, D. A. Andrade De
Giovambattista, Guillermo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Ciencias Agrarias
Buffalo
Microsatellite
Variability
Dna markers
Traceability
topic Ciencias Veterinarias
Ciencias Agrarias
Buffalo
Microsatellite
Variability
Dna markers
Traceability
dc.description.none.fl_txt_mv Buffaloes (Bubalus bubalis) are widely distributed and were introduced to Brazil in 1895. Most of the molecular genetic characterization of buffaloes has been done with cross-specific (cattle) markers, but few of them include Brazilian populations. Nineteen commonly used cattle microsatellites were tested to develop a multiplexed set of microsatellites and characterize Brazilian buffalo. Three PCR mixes were finally developed with the 11 markers that succeed in amplify and were polymorphic (58%). The average number of alleles was 5.42, with an average observed and expected heterocigozity of 0.441 and 0.695, respectively. As it was expected, Brazilian buffalo variability was lower than the previously reported from the domestication centres (China and India), but higher than the seriously selected European populations. The exclusion power calculated for the eleven markers in Brazilian buffalo was 0.9999999996, this allows its use in DNA based traceability.
Instituto de Genética Veterinaria
description Buffaloes (Bubalus bubalis) are widely distributed and were introduced to Brazil in 1895. Most of the molecular genetic characterization of buffaloes has been done with cross-specific (cattle) markers, but few of them include Brazilian populations. Nineteen commonly used cattle microsatellites were tested to develop a multiplexed set of microsatellites and characterize Brazilian buffalo. Three PCR mixes were finally developed with the 11 markers that succeed in amplify and were polymorphic (58%). The average number of alleles was 5.42, with an average observed and expected heterocigozity of 0.441 and 0.695, respectively. As it was expected, Brazilian buffalo variability was lower than the previously reported from the domestication centres (China and India), but higher than the seriously selected European populations. The exclusion power calculated for the eleven markers in Brazilian buffalo was 0.9999999996, this allows its use in DNA based traceability.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/96644
url http://sedici.unlp.edu.ar/handle/10915/96644
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/79628
info:eu-repo/semantics/altIdentifier/url/http://www.davidpublisher.org/index.php/Home/Article/index?id=15917.html
info:eu-repo/semantics/altIdentifier/issn/2161-6264
info:eu-repo/semantics/altIdentifier/hdl/11336/79628
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
dc.format.none.fl_str_mv application/pdf
1008-1012
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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