Corticostriatal cocaine-seeking ensembles are defined by differing gene expression from sucrose-seeking ensembles using a within-subject dual self-administration and seeking mouse...

Autores
Litif, Carl G.; Flom, Levi T.; Sandum, Kathryn L.; Hodgins, Skylar L.; Vaccaro, Lucio; Stitzel, Jerry A.; Ungerleider, Nathan; Mannino, María Constanza; Gigley, Jason P.; Schoborg, Todd A.; Bobadilla, Ana Clara
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Recurrent cocaine seeking is a hallmark of cocaine use disorder. To develop therapeutic targets, it is critical to understand the neurobiological changes specific to cocaine-seeking in context with the seeking of non-drug rewards, e.g., sucrose. The nucleus accumbens (NAc) and medial prefrontal cortex (mPFC) are known regions associated with cocaine- and sucrose-seeking ensembles, i.e., a sparse population of co-activated neurons linked with behavior. Within ensembles, transcriptomic alterations in the NAc and mPFC underlie the learning and recall of cocaine- and sucrose-seeking behavior. However, the transcriptomics exclusively driving cocaine seeking independent from sucrose seeking have not yet been defined using a within-subject approach. Using Ai14:cFos-TRAP2 transgenic mice in a dual cocaine and sucrose self-administration model, we fluorescently sorted and characterized the transcriptomes defining cocaine-seeking in reference to the sucrose-seeking ensemble, overlapping ensemble in between cocaine and sucrose-seeking, and the non-ensemble population. Our data suggests there are robust transcriptomic changes linked with cocaine-seeking that differ from sucroseseeking ensembles and the non-ensemble population which could guide future studies aimed to detangle cocaineseeking behavior without altering non-drug reward seeking.
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Ciencias Médicas
Farmacia
Cocaine
Sucrose
Neuronal ensembles
RNAsequencing
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/189513
Acceder
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Corticostriatal cocaine-seeking ensembles are defined by differing gene expression from sucrose-seeking ensembles using a within-subject dual self-administration and seeking mouse modelLitif, Carl G.Flom, Levi T.Sandum, Kathryn L.Hodgins, Skylar L.Vaccaro, LucioStitzel, Jerry A.Ungerleider, NathanMannino, María ConstanzaGigley, Jason P.Schoborg, Todd A.Bobadilla, Ana ClaraCiencias MédicasFarmaciaCocaineSucroseNeuronal ensemblesRNAsequencingRecurrent cocaine seeking is a hallmark of cocaine use disorder. To develop therapeutic targets, it is critical to understand the neurobiological changes specific to cocaine-seeking in context with the seeking of non-drug rewards, e.g., sucrose. The nucleus accumbens (NAc) and medial prefrontal cortex (mPFC) are known regions associated with cocaine- and sucrose-seeking ensembles, i.e., a sparse population of co-activated neurons linked with behavior. Within ensembles, transcriptomic alterations in the NAc and mPFC underlie the learning and recall of cocaine- and sucrose-seeking behavior. However, the transcriptomics exclusively driving cocaine seeking independent from sucrose seeking have not yet been defined using a within-subject approach. Using Ai14:cFos-TRAP2 transgenic mice in a dual cocaine and sucrose self-administration model, we fluorescently sorted and characterized the transcriptomes defining cocaine-seeking in reference to the sucrose-seeking ensemble, overlapping ensemble in between cocaine and sucrose-seeking, and the non-ensemble population. Our data suggests there are robust transcriptomic changes linked with cocaine-seeking that differ from sucroseseeking ensembles and the non-ensemble population which could guide future studies aimed to detangle cocaineseeking behavior without altering non-drug reward seeking.Instituto de Investigaciones Bioquímicas de La Plata2025-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/189513enginfo:eu-repo/semantics/altIdentifier/issn/2772-3925info:eu-repo/semantics/altIdentifier/doi/10.1016/j.addicn.2025.100242info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2026-03-26T09:20:51Zoai:sedici.unlp.edu.ar:10915/189513Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292026-03-26 09:20:51.918SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Corticostriatal cocaine-seeking ensembles are defined by differing gene expression from sucrose-seeking ensembles using a within-subject dual self-administration and seeking mouse model
title Corticostriatal cocaine-seeking ensembles are defined by differing gene expression from sucrose-seeking ensembles using a within-subject dual self-administration and seeking mouse model
spellingShingle Corticostriatal cocaine-seeking ensembles are defined by differing gene expression from sucrose-seeking ensembles using a within-subject dual self-administration and seeking mouse model
Litif, Carl G.
Ciencias Médicas
Farmacia
Cocaine
Sucrose
Neuronal ensembles
RNAsequencing
title_short Corticostriatal cocaine-seeking ensembles are defined by differing gene expression from sucrose-seeking ensembles using a within-subject dual self-administration and seeking mouse model
title_full Corticostriatal cocaine-seeking ensembles are defined by differing gene expression from sucrose-seeking ensembles using a within-subject dual self-administration and seeking mouse model
title_fullStr Corticostriatal cocaine-seeking ensembles are defined by differing gene expression from sucrose-seeking ensembles using a within-subject dual self-administration and seeking mouse model
title_full_unstemmed Corticostriatal cocaine-seeking ensembles are defined by differing gene expression from sucrose-seeking ensembles using a within-subject dual self-administration and seeking mouse model
title_sort Corticostriatal cocaine-seeking ensembles are defined by differing gene expression from sucrose-seeking ensembles using a within-subject dual self-administration and seeking mouse model
dc.creator.none.fl_str_mv Litif, Carl G.
Flom, Levi T.
Sandum, Kathryn L.
Hodgins, Skylar L.
Vaccaro, Lucio
Stitzel, Jerry A.
Ungerleider, Nathan
Mannino, María Constanza
Gigley, Jason P.
Schoborg, Todd A.
Bobadilla, Ana Clara
author Litif, Carl G.
author_facet Litif, Carl G.
Flom, Levi T.
Sandum, Kathryn L.
Hodgins, Skylar L.
Vaccaro, Lucio
Stitzel, Jerry A.
Ungerleider, Nathan
Mannino, María Constanza
Gigley, Jason P.
Schoborg, Todd A.
Bobadilla, Ana Clara
author_role author
author2 Flom, Levi T.
Sandum, Kathryn L.
Hodgins, Skylar L.
Vaccaro, Lucio
Stitzel, Jerry A.
Ungerleider, Nathan
Mannino, María Constanza
Gigley, Jason P.
Schoborg, Todd A.
Bobadilla, Ana Clara
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
Farmacia
Cocaine
Sucrose
Neuronal ensembles
RNAsequencing
topic Ciencias Médicas
Farmacia
Cocaine
Sucrose
Neuronal ensembles
RNAsequencing
dc.description.none.fl_txt_mv Recurrent cocaine seeking is a hallmark of cocaine use disorder. To develop therapeutic targets, it is critical to understand the neurobiological changes specific to cocaine-seeking in context with the seeking of non-drug rewards, e.g., sucrose. The nucleus accumbens (NAc) and medial prefrontal cortex (mPFC) are known regions associated with cocaine- and sucrose-seeking ensembles, i.e., a sparse population of co-activated neurons linked with behavior. Within ensembles, transcriptomic alterations in the NAc and mPFC underlie the learning and recall of cocaine- and sucrose-seeking behavior. However, the transcriptomics exclusively driving cocaine seeking independent from sucrose seeking have not yet been defined using a within-subject approach. Using Ai14:cFos-TRAP2 transgenic mice in a dual cocaine and sucrose self-administration model, we fluorescently sorted and characterized the transcriptomes defining cocaine-seeking in reference to the sucrose-seeking ensemble, overlapping ensemble in between cocaine and sucrose-seeking, and the non-ensemble population. Our data suggests there are robust transcriptomic changes linked with cocaine-seeking that differ from sucroseseeking ensembles and the non-ensemble population which could guide future studies aimed to detangle cocaineseeking behavior without altering non-drug reward seeking.
Instituto de Investigaciones Bioquímicas de La Plata
description Recurrent cocaine seeking is a hallmark of cocaine use disorder. To develop therapeutic targets, it is critical to understand the neurobiological changes specific to cocaine-seeking in context with the seeking of non-drug rewards, e.g., sucrose. The nucleus accumbens (NAc) and medial prefrontal cortex (mPFC) are known regions associated with cocaine- and sucrose-seeking ensembles, i.e., a sparse population of co-activated neurons linked with behavior. Within ensembles, transcriptomic alterations in the NAc and mPFC underlie the learning and recall of cocaine- and sucrose-seeking behavior. However, the transcriptomics exclusively driving cocaine seeking independent from sucrose seeking have not yet been defined using a within-subject approach. Using Ai14:cFos-TRAP2 transgenic mice in a dual cocaine and sucrose self-administration model, we fluorescently sorted and characterized the transcriptomes defining cocaine-seeking in reference to the sucrose-seeking ensemble, overlapping ensemble in between cocaine and sucrose-seeking, and the non-ensemble population. Our data suggests there are robust transcriptomic changes linked with cocaine-seeking that differ from sucroseseeking ensembles and the non-ensemble population which could guide future studies aimed to detangle cocaineseeking behavior without altering non-drug reward seeking.
publishDate 2025
dc.date.none.fl_str_mv 2025-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/189513
url http://sedici.unlp.edu.ar/handle/10915/189513
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2772-3925
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.addicn.2025.100242
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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