Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
- Autores
- Suktham, Thirada; Jones, Andrew; Acquaviva, Agustín; Dennis, Gary R.; Shalliker, R. Andrew; Soliven, Arianne
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This study is based upon a recently established method for quantification of the antioxidant capacity of natural samples via a HPLC separation and a hyphenated selective detection (post-column derivatization with cupric reducing antioxidant capacity reagent) technique. This protocol demonstrated the main improvements to transform the quantitative protocol into a high-speed qualitative automated assay to screen samples for their potential total antioxidant capacity, typically performed via manual mixing of the sample and derivatisation and measured on a 96 well plate reader/bench top UV–Vis spectrophotometer. This approach with automated mixing is a more informative alternative for total antioxidant capacity as the antioxidant peaks are profiled for each sample within four minutes. This antioxidant profile may be used for routine analysis of raw materials and/or a guide for targeted approaches for structure elucidation for laboratories interested in early drug discovery, natural product research and the search of alternative antioxidant additives in consumer goods/therapeutics. This technique could also be used to monitor the stability, alteration or adulteration of manufactured goods containing antioxidants.
Fil: Suktham, Thirada. University of Western Sydney; Australia
Fil: Jones, Andrew. University of Western Sydney; Australia
Fil: Acquaviva, Agustín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Grupo Cromatografía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Dennis, Gary R.. University of Western Sydney; Australia
Fil: Shalliker, R. Andrew. University of Western Sydney; Australia
Fil: Soliven, Arianne. University of Western Sydney; Australia. Universidad de la República; Uruguay - Materia
-
CUPRIC REDUCING ANTIOXIDANT CAPACITY
POST COLUMN DERIVATISATION
REACTION FLOW CHROMATOGRAPHY
SELECTIVE DETECTION
TOTAL ANTIOXIDANT CAPACITY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/130950
Ver los metadatos del registro completo
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Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatographySuktham, ThiradaJones, AndrewAcquaviva, AgustínDennis, Gary R.Shalliker, R. AndrewSoliven, ArianneCUPRIC REDUCING ANTIOXIDANT CAPACITYPOST COLUMN DERIVATISATIONREACTION FLOW CHROMATOGRAPHYSELECTIVE DETECTIONTOTAL ANTIOXIDANT CAPACITYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This study is based upon a recently established method for quantification of the antioxidant capacity of natural samples via a HPLC separation and a hyphenated selective detection (post-column derivatization with cupric reducing antioxidant capacity reagent) technique. This protocol demonstrated the main improvements to transform the quantitative protocol into a high-speed qualitative automated assay to screen samples for their potential total antioxidant capacity, typically performed via manual mixing of the sample and derivatisation and measured on a 96 well plate reader/bench top UV–Vis spectrophotometer. This approach with automated mixing is a more informative alternative for total antioxidant capacity as the antioxidant peaks are profiled for each sample within four minutes. This antioxidant profile may be used for routine analysis of raw materials and/or a guide for targeted approaches for structure elucidation for laboratories interested in early drug discovery, natural product research and the search of alternative antioxidant additives in consumer goods/therapeutics. This technique could also be used to monitor the stability, alteration or adulteration of manufactured goods containing antioxidants.Fil: Suktham, Thirada. University of Western Sydney; AustraliaFil: Jones, Andrew. University of Western Sydney; AustraliaFil: Acquaviva, Agustín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Grupo Cromatografía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Dennis, Gary R.. University of Western Sydney; AustraliaFil: Shalliker, R. Andrew. University of Western Sydney; AustraliaFil: Soliven, Arianne. University of Western Sydney; Australia. Universidad de la República; UruguayElsevier Science2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/130950Suktham, Thirada; Jones, Andrew; Acquaviva, Agustín; Dennis, Gary R.; Shalliker, R. Andrew; et al.; Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography; Elsevier Science; Microchemical Journal; 152; 1-2020; 1-220026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2019.104348info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0026265X19320302info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:29Zoai:ri.conicet.gov.ar:11336/130950instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:30.174CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography |
title |
Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography |
spellingShingle |
Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography Suktham, Thirada CUPRIC REDUCING ANTIOXIDANT CAPACITY POST COLUMN DERIVATISATION REACTION FLOW CHROMATOGRAPHY SELECTIVE DETECTION TOTAL ANTIOXIDANT CAPACITY |
title_short |
Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography |
title_full |
Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography |
title_fullStr |
Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography |
title_full_unstemmed |
Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography |
title_sort |
Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography |
dc.creator.none.fl_str_mv |
Suktham, Thirada Jones, Andrew Acquaviva, Agustín Dennis, Gary R. Shalliker, R. Andrew Soliven, Arianne |
author |
Suktham, Thirada |
author_facet |
Suktham, Thirada Jones, Andrew Acquaviva, Agustín Dennis, Gary R. Shalliker, R. Andrew Soliven, Arianne |
author_role |
author |
author2 |
Jones, Andrew Acquaviva, Agustín Dennis, Gary R. Shalliker, R. Andrew Soliven, Arianne |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
CUPRIC REDUCING ANTIOXIDANT CAPACITY POST COLUMN DERIVATISATION REACTION FLOW CHROMATOGRAPHY SELECTIVE DETECTION TOTAL ANTIOXIDANT CAPACITY |
topic |
CUPRIC REDUCING ANTIOXIDANT CAPACITY POST COLUMN DERIVATISATION REACTION FLOW CHROMATOGRAPHY SELECTIVE DETECTION TOTAL ANTIOXIDANT CAPACITY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
This study is based upon a recently established method for quantification of the antioxidant capacity of natural samples via a HPLC separation and a hyphenated selective detection (post-column derivatization with cupric reducing antioxidant capacity reagent) technique. This protocol demonstrated the main improvements to transform the quantitative protocol into a high-speed qualitative automated assay to screen samples for their potential total antioxidant capacity, typically performed via manual mixing of the sample and derivatisation and measured on a 96 well plate reader/bench top UV–Vis spectrophotometer. This approach with automated mixing is a more informative alternative for total antioxidant capacity as the antioxidant peaks are profiled for each sample within four minutes. This antioxidant profile may be used for routine analysis of raw materials and/or a guide for targeted approaches for structure elucidation for laboratories interested in early drug discovery, natural product research and the search of alternative antioxidant additives in consumer goods/therapeutics. This technique could also be used to monitor the stability, alteration or adulteration of manufactured goods containing antioxidants. Fil: Suktham, Thirada. University of Western Sydney; Australia Fil: Jones, Andrew. University of Western Sydney; Australia Fil: Acquaviva, Agustín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Grupo Cromatografía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Dennis, Gary R.. University of Western Sydney; Australia Fil: Shalliker, R. Andrew. University of Western Sydney; Australia Fil: Soliven, Arianne. University of Western Sydney; Australia. Universidad de la República; Uruguay |
description |
This study is based upon a recently established method for quantification of the antioxidant capacity of natural samples via a HPLC separation and a hyphenated selective detection (post-column derivatization with cupric reducing antioxidant capacity reagent) technique. This protocol demonstrated the main improvements to transform the quantitative protocol into a high-speed qualitative automated assay to screen samples for their potential total antioxidant capacity, typically performed via manual mixing of the sample and derivatisation and measured on a 96 well plate reader/bench top UV–Vis spectrophotometer. This approach with automated mixing is a more informative alternative for total antioxidant capacity as the antioxidant peaks are profiled for each sample within four minutes. This antioxidant profile may be used for routine analysis of raw materials and/or a guide for targeted approaches for structure elucidation for laboratories interested in early drug discovery, natural product research and the search of alternative antioxidant additives in consumer goods/therapeutics. This technique could also be used to monitor the stability, alteration or adulteration of manufactured goods containing antioxidants. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/130950 Suktham, Thirada; Jones, Andrew; Acquaviva, Agustín; Dennis, Gary R.; Shalliker, R. Andrew; et al.; Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography; Elsevier Science; Microchemical Journal; 152; 1-2020; 1-22 0026-265X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/130950 |
identifier_str_mv |
Suktham, Thirada; Jones, Andrew; Acquaviva, Agustín; Dennis, Gary R.; Shalliker, R. Andrew; et al.; Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography; Elsevier Science; Microchemical Journal; 152; 1-2020; 1-22 0026-265X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2019.104348 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0026265X19320302 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614519054139392 |
score |
13.070432 |