Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography

Autores
Suktham, Thirada; Jones, Andrew; Acquaviva, Agustín; Dennis, Gary R.; Shalliker, R. Andrew; Soliven, Arianne
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study is based upon a recently established method for quantification of the antioxidant capacity of natural samples via a HPLC separation and a hyphenated selective detection (post-column derivatization with cupric reducing antioxidant capacity reagent) technique. This protocol demonstrated the main improvements to transform the quantitative protocol into a high-speed qualitative automated assay to screen samples for their potential total antioxidant capacity, typically performed via manual mixing of the sample and derivatisation and measured on a 96 well plate reader/bench top UV–Vis spectrophotometer. This approach with automated mixing is a more informative alternative for total antioxidant capacity as the antioxidant peaks are profiled for each sample within four minutes. This antioxidant profile may be used for routine analysis of raw materials and/or a guide for targeted approaches for structure elucidation for laboratories interested in early drug discovery, natural product research and the search of alternative antioxidant additives in consumer goods/therapeutics. This technique could also be used to monitor the stability, alteration or adulteration of manufactured goods containing antioxidants.
Fil: Suktham, Thirada. University of Western Sydney; Australia
Fil: Jones, Andrew. University of Western Sydney; Australia
Fil: Acquaviva, Agustín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Grupo Cromatografía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Dennis, Gary R.. University of Western Sydney; Australia
Fil: Shalliker, R. Andrew. University of Western Sydney; Australia
Fil: Soliven, Arianne. University of Western Sydney; Australia. Universidad de la República; Uruguay
Materia
CUPRIC REDUCING ANTIOXIDANT CAPACITY
POST COLUMN DERIVATISATION
REACTION FLOW CHROMATOGRAPHY
SELECTIVE DETECTION
TOTAL ANTIOXIDANT CAPACITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/130950
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatographySuktham, ThiradaJones, AndrewAcquaviva, AgustínDennis, Gary R.Shalliker, R. AndrewSoliven, ArianneCUPRIC REDUCING ANTIOXIDANT CAPACITYPOST COLUMN DERIVATISATIONREACTION FLOW CHROMATOGRAPHYSELECTIVE DETECTIONTOTAL ANTIOXIDANT CAPACITYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This study is based upon a recently established method for quantification of the antioxidant capacity of natural samples via a HPLC separation and a hyphenated selective detection (post-column derivatization with cupric reducing antioxidant capacity reagent) technique. This protocol demonstrated the main improvements to transform the quantitative protocol into a high-speed qualitative automated assay to screen samples for their potential total antioxidant capacity, typically performed via manual mixing of the sample and derivatisation and measured on a 96 well plate reader/bench top UV–Vis spectrophotometer. This approach with automated mixing is a more informative alternative for total antioxidant capacity as the antioxidant peaks are profiled for each sample within four minutes. This antioxidant profile may be used for routine analysis of raw materials and/or a guide for targeted approaches for structure elucidation for laboratories interested in early drug discovery, natural product research and the search of alternative antioxidant additives in consumer goods/therapeutics. This technique could also be used to monitor the stability, alteration or adulteration of manufactured goods containing antioxidants.Fil: Suktham, Thirada. University of Western Sydney; AustraliaFil: Jones, Andrew. University of Western Sydney; AustraliaFil: Acquaviva, Agustín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Grupo Cromatografía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Dennis, Gary R.. University of Western Sydney; AustraliaFil: Shalliker, R. Andrew. University of Western Sydney; AustraliaFil: Soliven, Arianne. University of Western Sydney; Australia. Universidad de la República; UruguayElsevier Science2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/130950Suktham, Thirada; Jones, Andrew; Acquaviva, Agustín; Dennis, Gary R.; Shalliker, R. Andrew; et al.; Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography; Elsevier Science; Microchemical Journal; 152; 1-2020; 1-220026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2019.104348info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0026265X19320302info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:20:56Zoai:ri.conicet.gov.ar:11336/130950instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:20:56.659CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
title Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
spellingShingle Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
Suktham, Thirada
CUPRIC REDUCING ANTIOXIDANT CAPACITY
POST COLUMN DERIVATISATION
REACTION FLOW CHROMATOGRAPHY
SELECTIVE DETECTION
TOTAL ANTIOXIDANT CAPACITY
title_short Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
title_full Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
title_fullStr Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
title_full_unstemmed Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
title_sort Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
dc.creator.none.fl_str_mv Suktham, Thirada
Jones, Andrew
Acquaviva, Agustín
Dennis, Gary R.
Shalliker, R. Andrew
Soliven, Arianne
author Suktham, Thirada
author_facet Suktham, Thirada
Jones, Andrew
Acquaviva, Agustín
Dennis, Gary R.
Shalliker, R. Andrew
Soliven, Arianne
author_role author
author2 Jones, Andrew
Acquaviva, Agustín
Dennis, Gary R.
Shalliker, R. Andrew
Soliven, Arianne
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CUPRIC REDUCING ANTIOXIDANT CAPACITY
POST COLUMN DERIVATISATION
REACTION FLOW CHROMATOGRAPHY
SELECTIVE DETECTION
TOTAL ANTIOXIDANT CAPACITY
topic CUPRIC REDUCING ANTIOXIDANT CAPACITY
POST COLUMN DERIVATISATION
REACTION FLOW CHROMATOGRAPHY
SELECTIVE DETECTION
TOTAL ANTIOXIDANT CAPACITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study is based upon a recently established method for quantification of the antioxidant capacity of natural samples via a HPLC separation and a hyphenated selective detection (post-column derivatization with cupric reducing antioxidant capacity reagent) technique. This protocol demonstrated the main improvements to transform the quantitative protocol into a high-speed qualitative automated assay to screen samples for their potential total antioxidant capacity, typically performed via manual mixing of the sample and derivatisation and measured on a 96 well plate reader/bench top UV–Vis spectrophotometer. This approach with automated mixing is a more informative alternative for total antioxidant capacity as the antioxidant peaks are profiled for each sample within four minutes. This antioxidant profile may be used for routine analysis of raw materials and/or a guide for targeted approaches for structure elucidation for laboratories interested in early drug discovery, natural product research and the search of alternative antioxidant additives in consumer goods/therapeutics. This technique could also be used to monitor the stability, alteration or adulteration of manufactured goods containing antioxidants.
Fil: Suktham, Thirada. University of Western Sydney; Australia
Fil: Jones, Andrew. University of Western Sydney; Australia
Fil: Acquaviva, Agustín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Grupo Cromatografía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Dennis, Gary R.. University of Western Sydney; Australia
Fil: Shalliker, R. Andrew. University of Western Sydney; Australia
Fil: Soliven, Arianne. University of Western Sydney; Australia. Universidad de la República; Uruguay
description This study is based upon a recently established method for quantification of the antioxidant capacity of natural samples via a HPLC separation and a hyphenated selective detection (post-column derivatization with cupric reducing antioxidant capacity reagent) technique. This protocol demonstrated the main improvements to transform the quantitative protocol into a high-speed qualitative automated assay to screen samples for their potential total antioxidant capacity, typically performed via manual mixing of the sample and derivatisation and measured on a 96 well plate reader/bench top UV–Vis spectrophotometer. This approach with automated mixing is a more informative alternative for total antioxidant capacity as the antioxidant peaks are profiled for each sample within four minutes. This antioxidant profile may be used for routine analysis of raw materials and/or a guide for targeted approaches for structure elucidation for laboratories interested in early drug discovery, natural product research and the search of alternative antioxidant additives in consumer goods/therapeutics. This technique could also be used to monitor the stability, alteration or adulteration of manufactured goods containing antioxidants.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/130950
Suktham, Thirada; Jones, Andrew; Acquaviva, Agustín; Dennis, Gary R.; Shalliker, R. Andrew; et al.; Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography; Elsevier Science; Microchemical Journal; 152; 1-2020; 1-22
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/130950
identifier_str_mv Suktham, Thirada; Jones, Andrew; Acquaviva, Agustín; Dennis, Gary R.; Shalliker, R. Andrew; et al.; Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography; Elsevier Science; Microchemical Journal; 152; 1-2020; 1-22
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2019.104348
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0026265X19320302
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782661337546752
score 12.982451

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